Mutagenetix > Incidental Mutation

Incidental Mutation 'R1824:Unc79'

206699

Institutional Source

Beutler Lab

Gene Symbol

Unc79

Ensembl Gene

ENSMUSG00000021198

Gene Name

unc-79 homolog

Synonyms

9030205A07Rik, Mlca3

MMRRC Submission

039852-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1824 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

102915118-103150324 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 103025579 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 322 (N322S)

Ref Sequence

ENSEMBL: ENSMUSP00000136332 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085079] [ENSMUST00000101099] [ENSMUST00000178001] [ENSMUST00000178076] [ENSMUST00000179002]

AlphaFold

Q0KK59

Predicted Effect

possibly damaging
Transcript: ENSMUST00000085079
AA Change: N198S

PolyPhen 2 Score 0.707 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000082156
Gene: ENSMUSG00000021198
AA Change: N198S

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	469	3.1e-223	PFAM
low complexity region	732	737	N/A	INTRINSIC
low complexity region	846	862	N/A	INTRINSIC
low complexity region	968	977	N/A	INTRINSIC
low complexity region	1114	1125	N/A	INTRINSIC
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1428	1440	N/A	INTRINSIC
low complexity region	1471	1476	N/A	INTRINSIC
low complexity region	1477	1489	N/A	INTRINSIC
low complexity region	1490	1504	N/A	INTRINSIC
low complexity region	1541	1556	N/A	INTRINSIC
low complexity region	1861	1870	N/A	INTRINSIC
low complexity region	2237	2246	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000101099
AA Change: N375S

PolyPhen 2 Score 0.955 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000098659
Gene: ENSMUSG00000021198
AA Change: N375S

Domain	Start	End	E-Value	Type
Pfam:UNC-79	113	646	1.2e-226	PFAM
low complexity region	909	914	N/A	INTRINSIC
low complexity region	1023	1039	N/A	INTRINSIC
low complexity region	1145	1154	N/A	INTRINSIC
low complexity region	1291	1302	N/A	INTRINSIC
low complexity region	1490	1502	N/A	INTRINSIC
low complexity region	1605	1617	N/A	INTRINSIC
low complexity region	1648	1653	N/A	INTRINSIC
low complexity region	1654	1666	N/A	INTRINSIC
low complexity region	1667	1681	N/A	INTRINSIC
low complexity region	1718	1733	N/A	INTRINSIC
low complexity region	1999	2008	N/A	INTRINSIC
low complexity region	2375	2384	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000178001
AA Change: N153S

PolyPhen 2 Score 0.063 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000137132
Gene: ENSMUSG00000021198
AA Change: N153S

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	80	1.2e-30	PFAM
Pfam:UNC-79	78	188	2.1e-53	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000178076
AA Change: N179S

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000136888
Gene: ENSMUSG00000021198
AA Change: N179S

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	450	4.2e-213	PFAM
low complexity region	713	718	N/A	INTRINSIC
low complexity region	827	843	N/A	INTRINSIC
low complexity region	949	958	N/A	INTRINSIC
low complexity region	1117	1128	N/A	INTRINSIC
low complexity region	1316	1328	N/A	INTRINSIC
low complexity region	1431	1443	N/A	INTRINSIC
low complexity region	1474	1479	N/A	INTRINSIC
low complexity region	1480	1492	N/A	INTRINSIC
low complexity region	1493	1507	N/A	INTRINSIC
low complexity region	1544	1559	N/A	INTRINSIC
low complexity region	1864	1873	N/A	INTRINSIC
low complexity region	2240	2249	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000178846

Predicted Effect

probably damaging
Transcript: ENSMUST00000179002
AA Change: N322S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000136332
Gene: ENSMUSG00000021198
AA Change: N322S

Domain	Start	End	E-Value	Type
Pfam:UNC-79	60	593	1.3e-226	PFAM
low complexity region	856	861	N/A	INTRINSIC
low complexity region	970	986	N/A	INTRINSIC
low complexity region	1092	1101	N/A	INTRINSIC
low complexity region	1260	1271	N/A	INTRINSIC
low complexity region	1509	1521	N/A	INTRINSIC
low complexity region	1624	1636	N/A	INTRINSIC
low complexity region	1667	1672	N/A	INTRINSIC
low complexity region	1673	1685	N/A	INTRINSIC
low complexity region	1686	1700	N/A	INTRINSIC
low complexity region	1737	1752	N/A	INTRINSIC
low complexity region	2057	2066	N/A	INTRINSIC
low complexity region	2433	2442	N/A	INTRINSIC

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.2%
20x: 92.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The NALCN channel is responsible for Na(+) leak currents. The protein encoded by this gene, along with UNC80, is an accessory subunit of the NALCN channel that contributes to the Ca(2+) sensitivity of the channel. [provided by RefSeq, Sep 2016]
PHENOTYPE: Homozygous mutation results in lethality within the first week after birth, mostly at P0 or P1. Pups fail to nurse and have no milk in stomachs resulting in weakness, inactivity and no weight gain. [provided by MGI curators]

Allele List at MGI

All alleles(2) : Targeted, knock-out(1) Chemically induced(1)

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930533K18Rik	T	C	10: 70,708,086 (GRCm39)		noncoding transcript	Het
Aadacl2fm1	C	A	3: 59,840,001 (GRCm39)	Y24*	probably null	Het
Abl1	T	A	2: 31,690,656 (GRCm39)	M706K	probably benign	Het
Abtb1	T	C	6: 88,813,536 (GRCm39)	T401A	probably benign	Het
Acd	A	G	8: 106,427,122 (GRCm39)	L96P	probably damaging	Het
Arsi	T	A	18: 61,045,369 (GRCm39)	W20R	probably damaging	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Asic3	C	T	5: 24,618,749 (GRCm39)	Q14*	probably null	Het
Asxl3	T	A	18: 22,655,125 (GRCm39)	I1045N	probably damaging	Het
Atr	T	C	9: 95,818,474 (GRCm39)	I2149T	probably damaging	Het
Begain	A	G	12: 108,999,025 (GRCm39)		probably null	Het
Brca2	C	T	5: 150,460,387 (GRCm39)	T554I	possibly damaging	Het
Brd10	T	C	19: 29,693,814 (GRCm39)	N1960S	probably damaging	Het
C1s2	C	T	6: 124,612,641 (GRCm39)	V11I	probably benign	Het
Cacna1i	T	C	15: 80,260,990 (GRCm39)	F1333L	possibly damaging	Het
Camsap2	T	C	1: 136,201,521 (GRCm39)	T662A	possibly damaging	Het
Cep164	C	T	9: 45,690,226 (GRCm39)	V1367M	probably damaging	Het
Cfap46	C	A	7: 139,219,518 (GRCm39)	A1316S	probably benign	Het
Cic	T	C	7: 24,987,691 (GRCm39)	S553P	probably damaging	Het
Cilk1	T	A	9: 78,065,144 (GRCm39)	D351E	probably benign	Het
Clcn2	C	T	16: 20,534,712 (GRCm39)	A12T	probably benign	Het
Clip2	A	G	5: 134,532,081 (GRCm39)	Y540H	probably benign	Het
Coil	A	G	11: 88,872,923 (GRCm39)	N428S	possibly damaging	Het
Cpxm1	A	C	2: 130,237,617 (GRCm39)	V196G	probably damaging	Het
Cr2	A	G	1: 194,839,624 (GRCm39)	V601A	probably damaging	Het
Cyp3a25	T	A	5: 145,921,763 (GRCm39)	K390N	probably damaging	Het
Dclre1a	T	C	19: 56,535,150 (GRCm39)		probably null	Het
Dennd4a	G	C	9: 64,766,640 (GRCm39)		probably null	Het
Dlg5	T	C	14: 24,199,512 (GRCm39)	H1464R	probably benign	Het
Dmac2	T	G	7: 25,324,217 (GRCm39)	M225R	probably damaging	Het
Dnah8	T	C	17: 30,950,154 (GRCm39)	V1991A	possibly damaging	Het
Dscam	A	C	16: 96,626,781 (GRCm39)	V376G	probably benign	Het
Dync1li1	A	G	9: 114,538,252 (GRCm39)	D203G	probably benign	Het
Eva1c	A	G	16: 90,663,331 (GRCm39)	T22A	probably benign	Het
Fam110b	A	G	4: 5,799,029 (GRCm39)	D149G	probably benign	Het
Fras1	A	T	5: 96,918,547 (GRCm39)	I3528F	probably damaging	Het
Fsip1	T	C	2: 118,063,389 (GRCm39)	D360G	probably damaging	Het
Galnt14	T	C	17: 74,016,934 (GRCm39)	T41A	probably benign	Het
Gdf3	T	A	6: 122,586,921 (GRCm39)	Q2L	probably benign	Het
Glrp1	A	G	1: 88,437,511 (GRCm39)		probably null	Het
Gm3336	C	G	8: 71,173,066 (GRCm39)		probably null	Het
Gm8674	C	T	13: 50,054,844 (GRCm39)		noncoding transcript	Het
Gnat1	A	G	9: 107,553,774 (GRCm39)	Y226H	probably damaging	Het
Grk5	T	C	19: 61,078,410 (GRCm39)	V489A	probably damaging	Het
H1f8	A	G	6: 115,925,719 (GRCm39)	Y1C	probably null	Het
Igfbpl1	C	A	4: 45,826,406 (GRCm39)	A130S	probably benign	Het
Impdh1	T	A	6: 29,205,087 (GRCm39)	D261V	probably benign	Het
Itgal	T	A	7: 126,913,232 (GRCm39)	S610T	probably damaging	Het
Jcad	A	G	18: 4,649,293 (GRCm39)	T55A	probably benign	Het
Jup	G	A	11: 100,264,963 (GRCm39)	R663*	probably null	Het
Kalrn	C	T	16: 34,114,585 (GRCm39)	G556D	probably damaging	Het
Krt81	T	C	15: 101,358,020 (GRCm39)	E411G	probably damaging	Het
Lcat	T	C	8: 106,666,520 (GRCm39)	E334G	probably damaging	Het
Lhcgr	T	C	17: 89,057,585 (GRCm39)	E302G	probably benign	Het
Magi1	A	G	6: 93,676,620 (GRCm39)	V913A	possibly damaging	Het
Mrpl19	A	T	6: 81,941,060 (GRCm39)		probably null	Het
Muc4	G	T	16: 32,576,307 (GRCm39)		probably benign	Het
Mycbp2	A	G	14: 103,489,945 (GRCm39)	V953A	possibly damaging	Het
Myo3a	G	A	2: 22,401,054 (GRCm39)	V600I	probably benign	Het
Ndufv3	G	A	17: 31,750,219 (GRCm39)	R467Q	probably damaging	Het
Ngef	A	G	1: 87,430,986 (GRCm39)		probably null	Het
Nisch	A	T	14: 30,898,389 (GRCm39)		probably benign	Het
Nlrp4c	G	A	7: 6,069,955 (GRCm39)		probably null	Het
Nup153	A	T	13: 46,867,223 (GRCm39)	S154T	probably damaging	Het
Obscn	A	G	11: 58,885,658 (GRCm39)		probably benign	Het
Or2t1	T	A	14: 14,328,774 (GRCm38)	L221Q	probably damaging	Het
Or4a74	G	T	2: 89,439,693 (GRCm39)	P251H	probably damaging	Het
Or6c219	T	C	10: 129,781,206 (GRCm39)	M242V	possibly damaging	Het
Otogl	T	C	10: 107,615,692 (GRCm39)	N1869S	probably benign	Het
Phf24	G	T	4: 42,934,661 (GRCm39)	C136F	probably damaging	Het
Phldb2	A	T	16: 45,646,374 (GRCm39)	V65E	probably benign	Het
Pkhd1	C	A	1: 20,417,681 (GRCm39)	G2490V	probably damaging	Het
Prl8a8	A	T	13: 27,692,433 (GRCm39)	M186K	probably damaging	Het
Qars1	T	C	9: 108,391,809 (GRCm39)	V70A	probably damaging	Het
Rac1	C	T	5: 143,502,980 (GRCm39)	V14I	probably benign	Het
Rapgef5	T	A	12: 117,652,419 (GRCm39)		probably null	Het
Slc16a12	G	A	19: 34,648,278 (GRCm39)	T405M	possibly damaging	Het
Slc17a6	A	G	7: 51,311,294 (GRCm39)	Y336C	probably damaging	Het
Slc30a9	T	C	5: 67,505,395 (GRCm39)	L441P	probably damaging	Het
Slc45a2	T	C	15: 11,022,172 (GRCm39)	S305P	probably damaging	Het
Sod3	G	T	5: 52,525,504 (GRCm39)	V68L	probably benign	Het
Sp1	T	G	15: 102,339,438 (GRCm39)	S773A	possibly damaging	Het
Spen	C	A	4: 141,200,096 (GRCm39)	G2821C	probably damaging	Het
Tagap1	A	G	17: 7,223,425 (GRCm39)	S424P	probably benign	Het
Tbc1d22a	T	C	15: 86,119,770 (GRCm39)	V22A	possibly damaging	Het
Tfec	C	T	6: 16,840,467 (GRCm39)		probably null	Het
Thsd7a	A	T	6: 12,409,041 (GRCm39)		probably null	Het
Tnfsf15	C	A	4: 63,651,588 (GRCm39)	G112V	probably benign	Het
Tnfsf9	A	G	17: 57,412,738 (GRCm39)	T103A	probably benign	Het
Tnxb	C	T	17: 34,911,307 (GRCm39)	R1537*	probably null	Het
Tpra1	A	G	6: 88,888,805 (GRCm39)	N329S	probably benign	Het
Ttc12	A	G	9: 49,368,184 (GRCm39)	F281S	probably damaging	Het
Unk	G	T	11: 115,921,268 (GRCm39)		probably benign	Het
Usp4	G	T	9: 108,225,207 (GRCm39)	G31W	probably damaging	Het
Vcan	G	T	13: 89,853,331 (GRCm39)	A543D	possibly damaging	Het
Vil1	A	C	1: 74,457,606 (GRCm39)	I80L	probably benign	Het
Vmn2r27	C	T	6: 124,208,593 (GRCm39)	G51S	probably benign	Het
Vpreb1a	A	G	16: 16,686,935 (GRCm39)		probably null	Het
Zbtb6	C	T	2: 37,319,829 (GRCm39)	C33Y	probably damaging	Het
Zfp330	A	T	8: 83,492,644 (GRCm39)	C189*	probably null	Het
Zfp942	T	A	17: 22,147,522 (GRCm39)	H369L	probably damaging	Het
Zfp943	T	A	17: 22,211,361 (GRCm39)	I149K	probably benign	Het
Zfyve19	G	A	2: 119,042,016 (GRCm39)	V162M	probably benign	Het

Other mutations in Unc79

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00718:Unc79	APN	12	103,135,906 (GRCm39)	missense	possibly damaging	0.68
IGL00835:Unc79	APN	12	103,108,149 (GRCm39)	splice site	probably benign
IGL00917:Unc79	APN	12	103,054,766 (GRCm39)	missense	possibly damaging	0.53
IGL01012:Unc79	APN	12	103,078,714 (GRCm39)	missense	probably damaging	1.00
IGL01121:Unc79	APN	12	103,131,890 (GRCm39)	missense	probably damaging	0.99
IGL01303:Unc79	APN	12	103,128,126 (GRCm39)	missense	possibly damaging	0.94
IGL01305:Unc79	APN	12	102,968,130 (GRCm39)	missense	probably damaging	0.99
IGL01315:Unc79	APN	12	103,054,780 (GRCm39)	missense	possibly damaging	0.66
IGL01388:Unc79	APN	12	103,136,018 (GRCm39)	splice site	probably benign
IGL01415:Unc79	APN	12	103,074,944 (GRCm39)	missense	probably damaging	1.00
IGL01447:Unc79	APN	12	103,045,177 (GRCm39)	missense	probably damaging	1.00
IGL01655:Unc79	APN	12	103,134,546 (GRCm39)	missense	probably benign	0.00
IGL01662:Unc79	APN	12	103,115,279 (GRCm39)	missense	possibly damaging	0.92
IGL01728:Unc79	APN	12	103,131,943 (GRCm39)	missense	probably damaging	0.98
IGL01767:Unc79	APN	12	103,108,256 (GRCm39)	missense	probably damaging	1.00
IGL02080:Unc79	APN	12	102,968,234 (GRCm39)	missense	probably damaging	1.00
IGL02115:Unc79	APN	12	102,964,933 (GRCm39)	missense	probably damaging	1.00
IGL02176:Unc79	APN	12	102,965,006 (GRCm39)	splice site	probably null
IGL02186:Unc79	APN	12	102,977,542 (GRCm39)	missense	probably benign	0.04
IGL02205:Unc79	APN	12	103,045,260 (GRCm39)	missense	probably damaging	1.00
IGL02337:Unc79	APN	12	103,122,705 (GRCm39)	splice site	probably benign
IGL02498:Unc79	APN	12	103,137,837 (GRCm39)	missense	probably damaging	0.99
IGL02508:Unc79	APN	12	103,078,535 (GRCm39)	missense	probably damaging	0.97
IGL02508:Unc79	APN	12	103,078,277 (GRCm39)	splice site	probably benign
IGL02557:Unc79	APN	12	103,148,418 (GRCm39)	splice site	probably benign
IGL02589:Unc79	APN	12	103,139,755 (GRCm39)	missense	probably damaging	1.00
IGL02611:Unc79	APN	12	103,131,967 (GRCm39)	missense	probably damaging	0.97
IGL02728:Unc79	APN	12	103,088,688 (GRCm39)	missense	possibly damaging	0.53
IGL02827:Unc79	APN	12	103,041,105 (GRCm39)	missense	possibly damaging	0.88
IGL03028:Unc79	APN	12	103,139,785 (GRCm39)	missense	possibly damaging	0.83
IGL03144:Unc79	APN	12	103,008,401 (GRCm39)	missense	probably damaging	1.00
IGL03229:Unc79	APN	12	103,100,798 (GRCm39)	missense	probably damaging	0.99
IGL03269:Unc79	APN	12	103,054,936 (GRCm39)	missense	probably damaging	1.00
IGL03325:Unc79	APN	12	103,135,869 (GRCm39)	missense	probably damaging	0.98
pencil-thin	UTSW	12	103,075,040 (GRCm39)	splice site	probably null
sweetpea	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
3-1:Unc79	UTSW	12	103,039,009 (GRCm39)	nonsense	probably null
ANU22:Unc79	UTSW	12	102,968,130 (GRCm39)	missense	probably damaging	0.99
R0046:Unc79	UTSW	12	103,091,940 (GRCm39)	missense	probably damaging	0.99
R0046:Unc79	UTSW	12	103,091,940 (GRCm39)	missense	probably damaging	0.99
R0067:Unc79	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
R0067:Unc79	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
R0107:Unc79	UTSW	12	103,100,784 (GRCm39)	missense	possibly damaging	0.70
R0110:Unc79	UTSW	12	103,045,329 (GRCm39)	critical splice donor site	probably null
R0128:Unc79	UTSW	12	103,054,693 (GRCm39)	splice site	probably benign
R0166:Unc79	UTSW	12	103,122,812 (GRCm39)	missense	probably damaging	1.00
R0208:Unc79	UTSW	12	103,058,286 (GRCm39)	missense	probably benign	0.00
R0211:Unc79	UTSW	12	103,039,051 (GRCm39)	missense	probably benign	0.01
R0211:Unc79	UTSW	12	103,039,051 (GRCm39)	missense	probably benign	0.01
R0218:Unc79	UTSW	12	103,075,040 (GRCm39)	splice site	probably null
R0244:Unc79	UTSW	12	103,079,150 (GRCm39)	missense	probably damaging	1.00
R0305:Unc79	UTSW	12	103,079,459 (GRCm39)	missense	probably benign	0.18
R0310:Unc79	UTSW	12	103,027,666 (GRCm39)	missense	probably damaging	1.00
R0325:Unc79	UTSW	12	103,137,903 (GRCm39)	missense	probably damaging	0.98
R0369:Unc79	UTSW	12	103,055,031 (GRCm39)	critical splice donor site	probably null
R0450:Unc79	UTSW	12	103,045,329 (GRCm39)	critical splice donor site	probably null
R0503:Unc79	UTSW	12	103,045,127 (GRCm39)	missense	probably benign	0.01
R0542:Unc79	UTSW	12	103,060,437 (GRCm39)	splice site	probably benign
R0845:Unc79	UTSW	12	103,139,703 (GRCm39)	splice site	probably benign
R0893:Unc79	UTSW	12	102,957,687 (GRCm39)	missense	probably damaging	1.00
R1078:Unc79	UTSW	12	103,041,112 (GRCm39)	missense	probably benign	0.03
R1148:Unc79	UTSW	12	103,078,926 (GRCm39)	missense	probably damaging	1.00
R1148:Unc79	UTSW	12	103,078,926 (GRCm39)	missense	probably damaging	1.00
R1159:Unc79	UTSW	12	103,013,311 (GRCm39)	splice site	probably benign
R1191:Unc79	UTSW	12	103,013,271 (GRCm39)	nonsense	probably null
R1307:Unc79	UTSW	12	103,036,335 (GRCm39)	missense	probably damaging	1.00
R1368:Unc79	UTSW	12	103,122,772 (GRCm39)	missense	probably damaging	1.00
R1476:Unc79	UTSW	12	103,149,784 (GRCm39)	missense	probably damaging	1.00
R1650:Unc79	UTSW	12	103,079,052 (GRCm39)	missense	possibly damaging	0.85
R1777:Unc79	UTSW	12	103,078,714 (GRCm39)	missense	probably damaging	1.00
R1796:Unc79	UTSW	12	103,109,005 (GRCm39)	missense	probably damaging	0.99
R1830:Unc79	UTSW	12	103,100,737 (GRCm39)	missense	probably damaging	1.00
R1927:Unc79	UTSW	12	103,135,951 (GRCm39)	missense	probably damaging	1.00
R1958:Unc79	UTSW	12	103,041,178 (GRCm39)	missense	probably benign	0.19
R1958:Unc79	UTSW	12	102,957,621 (GRCm39)	missense	probably damaging	1.00
R1980:Unc79	UTSW	12	102,977,538 (GRCm39)	nonsense	probably null
R2019:Unc79	UTSW	12	103,137,830 (GRCm39)	critical splice acceptor site	probably null
R2290:Unc79	UTSW	12	103,112,625 (GRCm39)	missense	probably damaging	1.00
R2939:Unc79	UTSW	12	102,957,684 (GRCm39)	missense	probably damaging	1.00
R2962:Unc79	UTSW	12	103,061,378 (GRCm39)	missense	possibly damaging	0.72
R3176:Unc79	UTSW	12	103,079,476 (GRCm39)	missense	probably damaging	1.00
R3276:Unc79	UTSW	12	103,079,476 (GRCm39)	missense	probably damaging	1.00
R3683:Unc79	UTSW	12	103,041,062 (GRCm39)	missense	probably benign	0.00
R3684:Unc79	UTSW	12	103,041,062 (GRCm39)	missense	probably benign	0.00
R3686:Unc79	UTSW	12	103,054,920 (GRCm39)	missense	probably damaging	1.00
R3760:Unc79	UTSW	12	103,058,964 (GRCm39)	missense	probably damaging	1.00
R4031:Unc79	UTSW	12	103,039,018 (GRCm39)	missense	possibly damaging	0.46
R4039:Unc79	UTSW	12	103,041,208 (GRCm39)	missense	possibly damaging	0.88
R4110:Unc79	UTSW	12	103,025,629 (GRCm39)	missense	probably damaging	1.00
R4113:Unc79	UTSW	12	103,025,629 (GRCm39)	missense	probably damaging	1.00
R4159:Unc79	UTSW	12	103,036,512 (GRCm39)	intron	probably benign
R4273:Unc79	UTSW	12	103,088,612 (GRCm39)	missense	probably damaging	0.99
R4292:Unc79	UTSW	12	103,149,703 (GRCm39)	missense	probably damaging	0.99
R4334:Unc79	UTSW	12	103,045,233 (GRCm39)	missense	probably benign
R4513:Unc79	UTSW	12	102,988,019 (GRCm39)	missense	probably damaging	1.00
R4562:Unc79	UTSW	12	102,957,720 (GRCm39)	missense	probably damaging	1.00
R4576:Unc79	UTSW	12	102,968,062 (GRCm39)	splice site	probably benign
R4645:Unc79	UTSW	12	103,079,081 (GRCm39)	missense	probably benign
R4758:Unc79	UTSW	12	103,128,080 (GRCm39)	nonsense	probably null
R4787:Unc79	UTSW	12	103,013,257 (GRCm39)	missense	probably damaging	1.00
R4852:Unc79	UTSW	12	103,139,725 (GRCm39)	missense	probably damaging	0.98
R4883:Unc79	UTSW	12	103,060,592 (GRCm39)	missense	probably damaging	0.99
R4898:Unc79	UTSW	12	103,128,079 (GRCm39)	missense	probably damaging	0.99
R4979:Unc79	UTSW	12	103,078,691 (GRCm39)	missense	probably benign
R5044:Unc79	UTSW	12	103,078,962 (GRCm39)	missense	probably benign	0.32
R5053:Unc79	UTSW	12	103,071,007 (GRCm39)	missense	probably damaging	1.00
R5061:Unc79	UTSW	12	103,134,700 (GRCm39)	missense	possibly damaging	0.94
R5075:Unc79	UTSW	12	103,041,213 (GRCm39)	missense	possibly damaging	0.63
R5101:Unc79	UTSW	12	103,078,769 (GRCm39)	missense	probably damaging	1.00
R5236:Unc79	UTSW	12	103,060,654 (GRCm39)	critical splice donor site	probably null
R5240:Unc79	UTSW	12	103,037,010 (GRCm39)	missense	probably damaging	0.99
R5383:Unc79	UTSW	12	103,070,886 (GRCm39)	missense	possibly damaging	0.53
R5461:Unc79	UTSW	12	103,078,397 (GRCm39)	missense	probably damaging	1.00
R5535:Unc79	UTSW	12	103,135,962 (GRCm39)	missense	possibly damaging	0.84
R5609:Unc79	UTSW	12	103,094,527 (GRCm39)	missense	probably benign
R5639:Unc79	UTSW	12	103,137,831 (GRCm39)	missense	probably damaging	1.00
R5704:Unc79	UTSW	12	102,968,202 (GRCm39)	missense	probably damaging	1.00
R5923:Unc79	UTSW	12	103,078,727 (GRCm39)	missense	probably damaging	1.00
R5925:Unc79	UTSW	12	103,091,989 (GRCm39)	splice site	probably null
R5975:Unc79	UTSW	12	103,091,885 (GRCm39)	missense	possibly damaging	0.53
R6047:Unc79	UTSW	12	103,027,717 (GRCm39)	missense	probably damaging	1.00
R6156:Unc79	UTSW	12	103,027,717 (GRCm39)	missense	probably damaging	1.00
R6175:Unc79	UTSW	12	103,149,708 (GRCm39)	missense	probably damaging	0.98
R6292:Unc79	UTSW	12	103,108,991 (GRCm39)	missense	possibly damaging	0.88
R6313:Unc79	UTSW	12	103,078,878 (GRCm39)	missense	probably damaging	1.00
R6391:Unc79	UTSW	12	102,987,269 (GRCm39)	missense	probably damaging	1.00
R6405:Unc79	UTSW	12	103,134,595 (GRCm39)	missense	probably damaging	0.97
R6416:Unc79	UTSW	12	103,097,905 (GRCm39)	missense	possibly damaging	0.86
R6467:Unc79	UTSW	12	103,139,771 (GRCm39)	missense	probably damaging	1.00
R6573:Unc79	UTSW	12	103,027,647 (GRCm39)	missense	probably damaging	1.00
R6614:Unc79	UTSW	12	102,957,689 (GRCm39)	missense	probably damaging	1.00
R6654:Unc79	UTSW	12	103,045,308 (GRCm39)	missense	probably damaging	1.00
R6654:Unc79	UTSW	12	103,045,307 (GRCm39)	missense	probably damaging	0.99
R6700:Unc79	UTSW	12	103,091,962 (GRCm39)	missense	possibly damaging	0.92
R6724:Unc79	UTSW	12	103,071,120 (GRCm39)	missense	probably damaging	1.00
R6819:Unc79	UTSW	12	103,108,267 (GRCm39)	missense	probably benign	0.12
R6869:Unc79	UTSW	12	103,079,331 (GRCm39)	missense	probably benign	0.33
R6879:Unc79	UTSW	12	103,115,046 (GRCm39)	splice site	probably null
R6942:Unc79	UTSW	12	103,088,704 (GRCm39)	critical splice donor site	probably null
R6961:Unc79	UTSW	12	103,079,174 (GRCm39)	missense	probably damaging	1.00
R6973:Unc79	UTSW	12	102,964,699 (GRCm39)	missense	possibly damaging	0.86
R6980:Unc79	UTSW	12	103,025,759 (GRCm39)	missense	probably damaging	1.00
R7124:Unc79	UTSW	12	103,027,652 (GRCm39)	missense	probably damaging	0.99
R7144:Unc79	UTSW	12	103,108,885 (GRCm39)	missense	probably benign	0.06
R7197:Unc79	UTSW	12	103,078,765 (GRCm39)	missense	probably benign
R7209:Unc79	UTSW	12	103,091,883 (GRCm39)	missense	probably benign
R7232:Unc79	UTSW	12	103,100,734 (GRCm39)	missense	possibly damaging	0.49
R7304:Unc79	UTSW	12	103,029,449 (GRCm39)	missense	probably damaging	1.00
R7354:Unc79	UTSW	12	103,108,961 (GRCm39)	missense	possibly damaging	0.79
R7384:Unc79	UTSW	12	103,137,837 (GRCm39)	missense	probably benign	0.11
R7400:Unc79	UTSW	12	103,070,889 (GRCm39)	missense	probably damaging	1.00
R7417:Unc79	UTSW	12	103,055,017 (GRCm39)	missense	possibly damaging	0.85
R7470:Unc79	UTSW	12	103,061,235 (GRCm39)	missense	probably damaging	1.00
R7842:Unc79	UTSW	12	103,058,313 (GRCm39)	missense	probably damaging	1.00
R8037:Unc79	UTSW	12	103,016,178 (GRCm39)	missense	probably damaging	1.00
R8041:Unc79	UTSW	12	103,054,726 (GRCm39)	missense	probably benign	0.06
R8146:Unc79	UTSW	12	103,036,416 (GRCm39)	missense	probably damaging	0.98
R8276:Unc79	UTSW	12	102,968,122 (GRCm39)	missense	possibly damaging	0.94
R8427:Unc79	UTSW	12	103,045,297 (GRCm39)	missense	probably benign	0.24
R8501:Unc79	UTSW	12	103,058,897 (GRCm39)	missense	probably damaging	1.00
R8510:Unc79	UTSW	12	103,070,898 (GRCm39)	missense	probably damaging	1.00
R8531:Unc79	UTSW	12	103,013,922 (GRCm39)	missense	probably damaging	1.00
R8531:Unc79	UTSW	12	103,049,855 (GRCm39)	missense	probably benign	0.13
R8795:Unc79	UTSW	12	103,074,513 (GRCm39)	missense	probably damaging	1.00
R9017:Unc79	UTSW	12	103,074,874 (GRCm39)	critical splice acceptor site	probably null
R9121:Unc79	UTSW	12	102,968,095 (GRCm39)	missense	probably damaging	1.00
R9196:Unc79	UTSW	12	103,078,613 (GRCm39)	missense	probably benign
R9443:Unc79	UTSW	12	103,037,035 (GRCm39)	missense	probably damaging	1.00
R9548:Unc79	UTSW	12	102,977,495 (GRCm39)	missense	probably damaging	1.00
R9600:Unc79	UTSW	12	103,135,972 (GRCm39)	missense	probably benign	0.07
R9767:Unc79	UTSW	12	103,079,234 (GRCm39)	missense	probably benign
R9787:Unc79	UTSW	12	103,112,620 (GRCm39)	missense	probably benign	0.00
RF010:Unc79	UTSW	12	103,079,046 (GRCm39)	missense	probably benign	0.17
X0017:Unc79	UTSW	12	103,074,520 (GRCm39)	missense	probably damaging	0.99
X0028:Unc79	UTSW	12	102,957,662 (GRCm39)	missense	probably damaging	1.00
Z1088:Unc79	UTSW	12	102,987,271 (GRCm39)	missense	probably damaging	1.00
Z1176:Unc79	UTSW	12	103,108,312 (GRCm39)	missense	probably benign	0.03
Z1176:Unc79	UTSW	12	103,054,937 (GRCm39)	missense	probably damaging	1.00
Z1177:Unc79	UTSW	12	103,131,948 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TTCTCAGCATCACGGTTCTATG -3'
(R):5'- ACACTCCCTGGTGTTGATGG -3'

Sequencing Primer
(F):5'- CCAGCCTCCTAATGCCG -3'
(R):5'- GGGGGTGGAGAGGTCTG -3'

Posted On

2014-06-23