Mutagenetix > Incidental Mutation

Incidental Mutation 'R1850:Or8k35'

207980

Institutional Source

Beutler Lab

Gene Symbol

Or8k35

Ensembl Gene

ENSMUSG00000111689

Gene Name

olfactory receptor family 8 subfamily K member 35

Synonyms

MOR192-4_p, GA_x6K02T2Q125-48079993-48079157, Olfr1082

MMRRC Submission

039874-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.398) question?

Stock #

R1850 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

86424229-86429153 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 86424448 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 241 (C241*)

Ref Sequence

ENSEMBL: ENSMUSP00000150706 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000215600]

AlphaFold

A0A1L1SUC9

Predicted Effect

probably null
Transcript: ENSMUST00000111578
AA Change: C241*

SMART Domains

Protein: ENSMUSP00000107204
Gene: ENSMUSG00000079239
AA Change: C241*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	41	290	5.6e-31	PFAM
Pfam:7tm_4	139	283	4.5e-45	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000111579
AA Change: C241*

SMART Domains

Protein: ENSMUSP00000107205
Gene: ENSMUSG00000079239
AA Change: C241*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	8.2e-48	PFAM
Pfam:7tm_1	41	290	1.3e-19	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215600
AA Change: C241*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.8%

Validation Efficiency

99% (67/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam28	T	A	14: 68,876,644 (GRCm39)	Q202L	probably benign	Het
Adgb	C	A	10: 10,318,246 (GRCm39)	V199F	probably damaging	Het
Aff1	G	T	5: 103,981,773 (GRCm39)	R645S	probably damaging	Het
Aoc1	C	T	6: 48,882,202 (GRCm39)	S48F	probably benign	Het
Atf6	A	T	1: 170,646,855 (GRCm39)	N339K	probably damaging	Het
Bpifb3	C	G	2: 153,771,264 (GRCm39)	S392C	possibly damaging	Het
Camk1d	T	C	2: 5,366,826 (GRCm39)	M130V	probably benign	Het
Ces1a	T	A	8: 93,753,954 (GRCm39)	N350Y	probably damaging	Het
Cfap57	G	A	4: 118,457,091 (GRCm39)	R453C	probably damaging	Het
Chd4	T	A	6: 125,098,619 (GRCm39)	N1532K	probably damaging	Het
Chd5	T	C	4: 152,454,990 (GRCm39)	L824P	probably damaging	Het
Ckap5	G	A	2: 91,426,058 (GRCm39)	R1306H	probably damaging	Het
Crybg1	A	G	10: 43,873,670 (GRCm39)	F1146S	probably damaging	Het
Dusp12	G	A	1: 170,708,198 (GRCm39)	T173M	probably benign	Het
Dync2h1	T	C	9: 7,001,448 (GRCm39)	T3854A	probably benign	Het
Echdc1	A	T	10: 29,220,599 (GRCm39)	I252F	probably damaging	Het
Emc1	A	T	4: 139,086,684 (GRCm39)		probably benign	Het
Fbn2	C	A	18: 58,172,377 (GRCm39)		probably benign	Het
Fsip2	T	A	2: 82,814,933 (GRCm39)	N3555K	possibly damaging	Het
Gabra1	T	A	11: 42,070,403 (GRCm39)	T20S	probably benign	Het
Igf1	A	C	10: 87,697,236 (GRCm39)	T2P	possibly damaging	Het
Insyn1	A	T	9: 58,406,392 (GRCm39)	M101L	probably benign	Het
Jcad	C	A	18: 4,675,730 (GRCm39)	T1164N	possibly damaging	Het
Kalrn	A	T	16: 33,796,293 (GRCm39)	S2830T	probably damaging	Het
Lepr	C	A	4: 101,590,620 (GRCm39)	A66E	possibly damaging	Het
Lmntd1	T	A	6: 145,359,206 (GRCm39)	M315L	probably benign	Het
Lrch3	A	G	16: 32,807,163 (GRCm39)	T479A	probably benign	Het
Macroh2a1	T	C	13: 56,244,052 (GRCm39)		probably benign	Het
Matr3	A	G	18: 35,715,110 (GRCm39)	N237D	probably damaging	Het
Mslnl	G	A	17: 25,961,908 (GRCm39)	V128M	probably damaging	Het
Mtif2	A	G	11: 29,490,683 (GRCm39)	I462M	probably benign	Het
Nrip1	T	C	16: 76,090,232 (GRCm39)	I442V	probably damaging	Het
Or11g25	G	A	14: 50,723,055 (GRCm39)	A47T	probably benign	Het
Or6b2b	T	A	1: 92,419,124 (GRCm39)	M118L	possibly damaging	Het
Otogl	T	A	10: 107,713,925 (GRCm39)	Y498F	probably damaging	Het
Pbx3	A	T	2: 34,066,832 (GRCm39)	F351I	probably benign	Het
Pcdh18	T	C	3: 49,710,854 (GRCm39)	T154A	probably benign	Het
Pex5l	A	T	3: 33,005,025 (GRCm39)		probably null	Het
Plec	T	C	15: 76,072,432 (GRCm39)	I718V	probably benign	Het
Pparg	T	A	6: 115,427,941 (GRCm39)	Y143N	probably damaging	Het
Prl2c5	A	G	13: 13,360,377 (GRCm39)	I12V	probably benign	Het
Rcor3	T	A	1: 191,804,411 (GRCm39)	Q246L	probably benign	Het
Rnf214	A	T	9: 45,780,746 (GRCm39)		probably benign	Het
S1pr5	A	T	9: 21,155,425 (GRCm39)	S334T	probably benign	Het
Scg3	C	T	9: 75,589,449 (GRCm39)	S35N	possibly damaging	Het
Septin5	A	T	16: 18,443,960 (GRCm39)	L19Q	probably damaging	Het
Serpinb7	T	C	1: 107,356,025 (GRCm39)	F16S	probably damaging	Het
Sipa1l3	A	T	7: 29,038,551 (GRCm39)	S365R	probably damaging	Het
Slc27a1	T	C	8: 72,033,347 (GRCm39)		probably null	Het
Slc2a10	C	A	2: 165,357,133 (GRCm39)	H264Q	probably benign	Het
Slc9a3	A	G	13: 74,309,889 (GRCm39)	I526V	probably benign	Het
Smchd1	T	C	17: 71,696,766 (GRCm39)	D1203G	probably damaging	Het
Spata6l	T	C	19: 28,916,571 (GRCm39)		probably null	Het
Sult1b1	A	G	5: 87,668,700 (GRCm39)	W181R	probably damaging	Het
Supt6	A	G	11: 78,110,703 (GRCm39)		probably benign	Het
Tcf12	C	A	9: 71,775,497 (GRCm39)	A418S	probably damaging	Het
Tesk1	C	T	4: 43,443,576 (GRCm39)	R48C	probably damaging	Het
Tiam2	A	G	17: 3,487,510 (GRCm39)	Q677R	probably damaging	Het
Tspan8	C	T	10: 115,669,130 (GRCm39)	A55V	probably damaging	Het
Txndc11	T	C	16: 10,906,268 (GRCm39)	N421D	probably damaging	Het
Vmn1r201	A	G	13: 22,658,801 (GRCm39)	N5S	probably benign	Het
Vmn2r120	T	A	17: 57,832,826 (GRCm39)	I118L	probably benign	Het
Vps13b	A	T	15: 35,675,105 (GRCm39)		probably benign	Het
Wdfy3	A	G	5: 102,042,865 (GRCm39)	V1962A	probably damaging	Het
Zswim8	C	T	14: 20,760,815 (GRCm39)	R107*	probably null	Het

Other mutations in Or8k35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0483:Or8k35	UTSW	2	86,424,752 (GRCm39)	missense	probably benign	0.07
R0675:Or8k35	UTSW	2	86,424,423 (GRCm39)	missense	probably benign	0.00
R0718:Or8k35	UTSW	2	86,424,425 (GRCm39)	missense	probably benign	0.01
R0727:Or8k35	UTSW	2	86,424,724 (GRCm39)	nonsense	probably null
R1517:Or8k35	UTSW	2	86,424,948 (GRCm39)	missense	probably damaging	0.99
R2430:Or8k35	UTSW	2	86,425,052 (GRCm39)	missense	probably benign	0.00
R2474:Or8k35	UTSW	2	86,424,957 (GRCm39)	missense	probably benign	0.00
R3009:Or8k35	UTSW	2	86,424,714 (GRCm39)	missense	probably benign	0.07
R3122:Or8k35	UTSW	2	86,424,954 (GRCm39)	missense	possibly damaging	0.91
R4006:Or8k35	UTSW	2	86,424,908 (GRCm39)	missense	probably benign	0.14
R4007:Or8k35	UTSW	2	86,424,908 (GRCm39)	missense	probably benign	0.14
R4581:Or8k35	UTSW	2	86,424,572 (GRCm39)	missense	probably benign	0.08
R4762:Or8k35	UTSW	2	86,424,381 (GRCm39)	missense	possibly damaging	0.60
R5617:Or8k35	UTSW	2	86,424,345 (GRCm39)	missense	probably benign	0.07
R6118:Or8k35	UTSW	2	86,424,758 (GRCm39)	missense	probably benign
R6140:Or8k35	UTSW	2	86,424,448 (GRCm39)	nonsense	probably null
R6313:Or8k35	UTSW	2	86,424,411 (GRCm39)	missense	possibly damaging	0.76
R6528:Or8k35	UTSW	2	86,424,809 (GRCm39)	missense	probably damaging	1.00
R6785:Or8k35	UTSW	2	86,424,765 (GRCm39)	missense	probably damaging	0.98
R6792:Or8k35	UTSW	2	86,424,283 (GRCm39)	missense	probably benign	0.09
R6857:Or8k35	UTSW	2	86,424,608 (GRCm39)	missense	probably damaging	1.00
R6998:Or8k35	UTSW	2	86,424,488 (GRCm39)	missense	probably damaging	1.00
R7241:Or8k35	UTSW	2	86,424,498 (GRCm39)	missense	possibly damaging	0.89
R8790:Or8k35	UTSW	2	86,424,278 (GRCm39)	missense	possibly damaging	0.76
R8865:Or8k35	UTSW	2	86,424,744 (GRCm39)	missense	possibly damaging	0.89
R9521:Or8k35	UTSW	2	86,424,771 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- GCCATGTTCTTTGTAGAGCATC -3'
(R):5'- TTCTGTGGCTACAATGTCATCAACC -3'

Sequencing Primer
(F):5'- CATGTTCTTTGTAGAGCATCTTTTAC -3'
(R):5'- GTGGCTACAATGTCATCAACCATTTC -3'

Posted On

2014-06-23