Incidental Mutation 'R1886:Lag3'
ID 209532
Institutional Source Beutler Lab
Gene Symbol Lag3
Ensembl Gene ENSMUSG00000030124
Gene Name lymphocyte-activation gene 3
Synonyms LAG-3, CD223, Ly66
MMRRC Submission 039907-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.091) question?
Stock # R1886 (G1)
Quality Score 225
Status Not validated
Chromosome 6
Chromosomal Location 124881324-124888668 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 124886402 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Aspartic acid at position 184 (N184D)
Ref Sequence ENSEMBL: ENSMUSP00000032217 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000032216] [ENSMUST00000032217]
AlphaFold Q61790
Predicted Effect probably benign
Transcript: ENSMUST00000032216
SMART Domains Protein: ENSMUSP00000032216
Gene: ENSMUSG00000030122

DomainStartEndE-ValueType
Pfam:Prothymosin 2 98 1e-17 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000032217
AA Change: N184D

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000032217
Gene: ENSMUSG00000030124
AA Change: N184D

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
IG 29 163 1.3e-2 SMART
IG 170 254 6.51e-3 SMART
IG 261 345 4.96e-8 SMART
Blast:IG_like 348 421 2e-28 BLAST
transmembrane domain 443 465 N/A INTRINSIC
low complexity region 492 521 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000139571
Predicted Effect noncoding transcript
Transcript: ENSMUST00000181150
Predicted Effect noncoding transcript
Transcript: ENSMUST00000203422
Predicted Effect noncoding transcript
Transcript: ENSMUST00000204671
Coding Region Coverage
  • 1x: 97.2%
  • 3x: 96.2%
  • 10x: 93.1%
  • 20x: 85.9%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene have a generally normal phenotype but do display reduced natural killer cell activity and increased T cell response to infection. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 87 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700102P08Rik T A 9: 108,270,809 (GRCm39) D124E possibly damaging Het
Aarsd1 T C 11: 101,302,227 (GRCm39) T278A probably benign Het
Acacb T A 5: 114,357,020 (GRCm39) L1317Q probably damaging Het
Acsf3 T C 8: 123,510,741 (GRCm39) V293A probably damaging Het
Adora3 A C 3: 105,812,152 (GRCm39) N13H possibly damaging Het
Aen A T 7: 78,557,073 (GRCm39) D307V probably damaging Het
Ahnak G A 19: 8,993,343 (GRCm39) D4876N probably damaging Het
Ap2b1 T A 11: 83,281,561 (GRCm39) N822K probably damaging Het
Arhgdia T C 11: 120,470,244 (GRCm39) D143G probably benign Het
Bcl9 G T 3: 97,122,713 (GRCm39) R29S probably benign Het
Bhmt2 T A 13: 93,798,998 (GRCm39) K274N probably benign Het
Brip1 C A 11: 86,029,641 (GRCm39) G631V probably damaging Het
Cacna1i A G 15: 80,243,145 (GRCm39) E434G probably damaging Het
Ccdc81 T C 7: 89,515,819 (GRCm39) E620G possibly damaging Het
Cdhr18 A C 14: 13,828,607 (GRCm38) Y718D probably damaging Het
Cfap299 A G 5: 98,949,690 (GRCm39) N208S probably benign Het
Cit A G 5: 116,071,545 (GRCm39) Y584C probably damaging Het
Crebl2 T C 6: 134,828,059 (GRCm39) M77T probably benign Het
Dach2 G A X: 112,208,305 (GRCm39) G61D probably benign Het
Ddx4 A T 13: 112,759,199 (GRCm39) D237E probably damaging Het
Dgcr8 A G 16: 18,096,218 (GRCm39) I490T possibly damaging Het
Dmxl1 G A 18: 49,992,202 (GRCm39) R316H probably benign Het
Dnah17 T A 11: 117,998,987 (GRCm39) I929F possibly damaging Het
Eif3f A G 7: 108,539,958 (GRCm39) T289A probably benign Het
Ercc5 A T 1: 44,215,136 (GRCm39) K890* probably null Het
Esrp2 A T 8: 106,860,489 (GRCm39) V247E probably damaging Het
Evpl C T 11: 116,118,402 (GRCm39) G735E probably damaging Het
Ezhip GTCATCATCATCATC GTCATCATCATCATCATC X: 5,994,645 (GRCm39) probably benign Het
Fbxl21 A G 13: 56,674,906 (GRCm39) I60V probably benign Het
Frem3 A G 8: 81,340,514 (GRCm39) I936V probably benign Het
Fsd1l T C 4: 53,696,984 (GRCm39) probably null Het
Gad1-ps T C 10: 99,281,444 (GRCm39) noncoding transcript Het
Gbp2b A G 3: 142,314,063 (GRCm39) T448A probably benign Het
Gfus G T 15: 75,798,838 (GRCm39) T123N possibly damaging Het
Gmppa T C 1: 75,419,152 (GRCm39) V353A probably damaging Het
Hmcn1 T C 1: 150,453,046 (GRCm39) E5423G probably benign Het
Hmgxb3 A G 18: 61,270,473 (GRCm39) probably null Het
Krt12 T C 11: 99,309,402 (GRCm39) D286G probably damaging Het
Krt75 A T 15: 101,479,532 (GRCm39) M266K probably damaging Het
Ksr1 C A 11: 78,911,204 (GRCm39) V11F probably null Het
Lsm10 C G 4: 125,991,741 (GRCm39) D32E probably benign Het
Mettl5 A G 2: 69,711,149 (GRCm39) V123A probably damaging Het
Mfrp A G 9: 44,014,785 (GRCm39) D274G possibly damaging Het
Mgat3 A G 15: 80,095,820 (GRCm39) I216V probably benign Het
Mkrn3 G A 7: 62,068,486 (GRCm39) A435V probably benign Het
Mob3a G A 10: 80,527,068 (GRCm39) Q86* probably null Het
Mplkipl1 A G 19: 61,164,136 (GRCm39) F100L probably damaging Het
Mttp A T 3: 137,798,376 (GRCm39) V840D probably damaging Het
Nadk2 A C 15: 9,103,446 (GRCm39) N308H possibly damaging Het
Ncoa7 T C 10: 30,524,448 (GRCm39) N823S possibly damaging Het
Nlk T A 11: 78,477,754 (GRCm39) I330F probably damaging Het
Ofcc1 T C 13: 40,360,100 (GRCm39) S310G possibly damaging Het
Or5p57 T C 7: 107,665,985 (GRCm39) N7D probably benign Het
Or5p73 A T 7: 108,064,947 (GRCm39) M139L probably benign Het
Orc2 T C 1: 58,510,247 (GRCm39) probably null Het
Orc3 A G 4: 34,584,829 (GRCm39) Y459H probably damaging Het
Pah T A 10: 87,364,190 (GRCm39) N30K possibly damaging Het
Pcsk4 T C 10: 80,164,794 (GRCm39) K74E probably benign Het
Pdlim7 C T 13: 55,653,981 (GRCm39) G212D probably benign Het
Pfkm A G 15: 98,025,627 (GRCm39) N547S probably damaging Het
Por A G 5: 135,763,128 (GRCm39) E546G probably damaging Het
Ppp1r13l T C 7: 19,111,496 (GRCm39) S774P probably damaging Het
Prdm1 T A 10: 44,315,754 (GRCm39) D794V probably damaging Het
Prdx5 A G 19: 6,885,558 (GRCm39) I32T probably benign Het
Prlhr G T 19: 60,455,932 (GRCm39) C211* probably null Het
Ripk3 C T 14: 56,025,694 (GRCm39) probably null Het
Rmnd5b G A 11: 51,518,465 (GRCm39) A137V probably damaging Het
Rwdd2b A T 16: 87,234,013 (GRCm39) F72I probably benign Het
Scml4 A G 10: 42,788,223 (GRCm39) Y51C probably damaging Het
Septin1 A G 7: 126,813,937 (GRCm39) probably benign Het
Serpinb9h C T 13: 33,588,203 (GRCm39) R263C possibly damaging Het
Slc30a10 T A 1: 185,195,061 (GRCm39) I291N probably damaging Het
Slc7a9 T G 7: 35,152,827 (GRCm39) C20W possibly damaging Het
Slc7a9 G C 7: 35,152,828 (GRCm39) A21P probably damaging Het
Slco1b2 A T 6: 141,628,951 (GRCm39) Y551F probably damaging Het
Sra1 T C 18: 36,801,830 (GRCm39) M87V probably benign Het
Syvn1 T C 19: 6,099,257 (GRCm39) S169P possibly damaging Het
Tmc7 A T 7: 118,160,310 (GRCm39) F176I possibly damaging Het
Tmem62 A T 2: 120,817,151 (GRCm39) I236F probably damaging Het
Trip4 T A 9: 65,782,163 (GRCm39) I190F probably null Het
Trpa1 T C 1: 14,959,649 (GRCm39) D679G probably benign Het
Ttc22 T A 4: 106,494,063 (GRCm39) probably null Het
Ttn A T 2: 76,641,587 (GRCm39) L5176Q possibly damaging Het
Tyrp1 T A 4: 80,759,043 (GRCm39) probably null Het
Ubap2 C T 4: 41,199,872 (GRCm39) A752T probably benign Het
Usp36 A T 11: 118,163,784 (GRCm39) Y255N probably damaging Het
Zfp944 A T 17: 22,558,960 (GRCm39) Y96N probably benign Het
Other mutations in Lag3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01128:Lag3 APN 6 124,886,380 (GRCm39) missense probably damaging 1.00
IGL01867:Lag3 APN 6 124,887,869 (GRCm39) missense probably benign
IGL02880:Lag3 APN 6 124,882,434 (GRCm39) missense probably benign 0.01
R1502:Lag3 UTSW 6 124,886,206 (GRCm39) missense probably damaging 1.00
R1573:Lag3 UTSW 6 124,886,210 (GRCm39) missense possibly damaging 0.65
R1907:Lag3 UTSW 6 124,886,450 (GRCm39) missense possibly damaging 0.52
R2508:Lag3 UTSW 6 124,888,272 (GRCm39) missense possibly damaging 0.91
R3016:Lag3 UTSW 6 124,885,429 (GRCm39) missense probably damaging 1.00
R3732:Lag3 UTSW 6 124,887,103 (GRCm39) missense probably benign 0.05
R3732:Lag3 UTSW 6 124,887,103 (GRCm39) missense probably benign 0.05
R3733:Lag3 UTSW 6 124,887,103 (GRCm39) missense probably benign 0.05
R3734:Lag3 UTSW 6 124,887,103 (GRCm39) missense probably benign 0.05
R4679:Lag3 UTSW 6 124,881,508 (GRCm39) missense possibly damaging 0.92
R4994:Lag3 UTSW 6 124,881,416 (GRCm39) missense unknown
R5057:Lag3 UTSW 6 124,882,318 (GRCm39) missense possibly damaging 0.58
R5527:Lag3 UTSW 6 124,885,592 (GRCm39) missense probably damaging 0.99
R7227:Lag3 UTSW 6 124,885,457 (GRCm39) missense possibly damaging 0.79
R7255:Lag3 UTSW 6 124,887,198 (GRCm39) missense probably benign 0.04
R8081:Lag3 UTSW 6 124,882,410 (GRCm39) nonsense probably null
R8138:Lag3 UTSW 6 124,882,455 (GRCm39) missense probably damaging 0.99
R8710:Lag3 UTSW 6 124,885,408 (GRCm39) missense probably damaging 1.00
R9126:Lag3 UTSW 6 124,881,809 (GRCm39) missense probably damaging 0.99
X0009:Lag3 UTSW 6 124,882,315 (GRCm39) missense possibly damaging 0.62
Predicted Primers PCR Primer
(F):5'- GGAGACATTGAAGCCATCTCTG -3'
(R):5'- CCCCAGGTTTCAAGAACTGAC -3'

Sequencing Primer
(F):5'- CATCTCTGTAGGTGAGGACAC -3'
(R):5'- TTTCAAGAACTGACTTGGAATGAGCG -3'
Posted On 2014-06-30