Incidental Mutation 'R1908:Or13a27'
ID 210059
Institutional Source Beutler Lab
Gene Symbol Or13a27
Ensembl Gene ENSMUSG00000060112
Gene Name olfactory receptor family 13 subfamily A member 27
Synonyms MOR253-6, Olfr60, GA_x6K02T2PBJ9-42496183-42495251, IH6
MMRRC Submission 039927-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.061) question?
Stock # R1908 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 139924965-139925900 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 139925378 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Valine at position 175 (I175V)
Ref Sequence ENSEMBL: ENSMUSP00000150685 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000075470] [ENSMUST00000210932] [ENSMUST00000211031] [ENSMUST00000215023] [ENSMUST00000216027]
AlphaFold Q8VGL4
Predicted Effect probably benign
Transcript: ENSMUST00000075470
AA Change: I175V

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000074916
Gene: ENSMUSG00000060112
AA Change: I175V

DomainStartEndE-ValueType
Pfam:7tm_4 32 308 1.2e-50 PFAM
Pfam:7TM_GPCR_Srsx 36 161 1.8e-8 PFAM
Pfam:7tm_1 42 291 2e-21 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000209726
Predicted Effect probably benign
Transcript: ENSMUST00000210932
AA Change: I175V

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)
Predicted Effect probably benign
Transcript: ENSMUST00000211031
AA Change: I175V

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000211655
Predicted Effect probably benign
Transcript: ENSMUST00000215023
AA Change: I175V

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)
Predicted Effect probably benign
Transcript: ENSMUST00000216027
AA Change: I175V

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)
Coding Region Coverage
  • 1x: 97.3%
  • 3x: 96.8%
  • 10x: 95.4%
  • 20x: 93.0%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 83 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca8b C A 11: 109,847,924 (GRCm39) L852F possibly damaging Het
Abcc6 T C 7: 45,669,558 (GRCm39) probably null Het
Alg11 T G 8: 22,555,584 (GRCm39) C240G probably damaging Het
Aox1 A G 1: 58,141,783 (GRCm39) I1190V probably damaging Het
Apc2 T G 10: 80,150,678 (GRCm39) S1911A probably benign Het
Arfgef3 T C 10: 18,528,511 (GRCm39) D292G possibly damaging Het
Arhgef4 C A 1: 34,763,340 (GRCm39) S865R probably benign Het
Ass1 T A 2: 31,383,160 (GRCm39) Y190* probably null Het
B4galnt3 A T 6: 120,187,051 (GRCm39) probably null Het
Btnl10 C A 11: 58,811,367 (GRCm39) P230Q possibly damaging Het
C3 A G 17: 57,516,489 (GRCm39) Y1348H probably damaging Het
Ccdc198 G T 14: 49,464,032 (GRCm39) D292E probably damaging Het
Cebpz A T 17: 79,242,336 (GRCm39) Y439* probably null Het
Cic C A 7: 24,986,265 (GRCm39) T1229K probably damaging Het
Clip4 T C 17: 72,144,744 (GRCm39) S524P probably damaging Het
Col6a3 A C 1: 90,739,421 (GRCm39) I269R probably damaging Het
Dbh C T 2: 27,071,506 (GRCm39) T533I possibly damaging Het
Dcaf17 A T 2: 70,890,713 (GRCm39) R83* probably null Het
Dennd2b G A 7: 109,124,533 (GRCm39) Q686* probably null Het
Dnah1 A G 14: 30,984,515 (GRCm39) L3923P probably damaging Het
Dnah7a A T 1: 53,670,721 (GRCm39) D510E probably benign Het
Dock6 A G 9: 21,752,925 (GRCm39) F296S probably damaging Het
Eif4enif1 T C 11: 3,177,455 (GRCm39) S341P probably damaging Het
Epb41l1 G T 2: 156,352,737 (GRCm39) G461V possibly damaging Het
Epg5 T A 18: 78,002,247 (GRCm39) D555E probably benign Het
Fes T C 7: 80,036,609 (GRCm39) R113G probably damaging Het
Garre1 T A 7: 33,957,461 (GRCm39) I59L probably benign Het
Gm10684 A G 9: 45,021,511 (GRCm39) probably benign Het
Gm12185 T C 11: 48,806,231 (GRCm39) E320G probably benign Het
Gm14412 G T 2: 177,007,269 (GRCm39) H209N probably damaging Het
Gm14412 A C 2: 177,007,630 (GRCm39) S88R probably benign Het
Grhl1 T A 12: 24,658,555 (GRCm39) L400Q probably damaging Het
Havcr1 T A 11: 46,664,511 (GRCm39) Y216* probably null Het
Hephl1 A C 9: 14,985,420 (GRCm39) Y745* probably null Het
Hmcn2 T G 2: 31,301,922 (GRCm39) probably null Het
Hs3st6 A G 17: 24,977,110 (GRCm39) K197E possibly damaging Het
Ifi214 C T 1: 173,357,077 (GRCm39) V9I probably benign Het
Jakmip2 T C 18: 43,700,209 (GRCm39) T450A probably benign Het
Lrp4 T A 2: 91,328,753 (GRCm39) V1551D possibly damaging Het
Macf1 A T 4: 123,351,634 (GRCm39) H1634Q possibly damaging Het
Mcpt8 T A 14: 56,321,291 (GRCm39) I58F probably benign Het
Mga T A 2: 119,757,075 (GRCm39) H1018Q possibly damaging Het
Myo10 T A 15: 25,801,308 (GRCm39) V1499E probably damaging Het
Myom2 G T 8: 15,131,023 (GRCm39) D320Y probably damaging Het
Or11g7 T A 14: 50,691,295 (GRCm39) M262K probably damaging Het
Or2r11 C T 6: 42,437,360 (GRCm39) V198M probably benign Het
Or2t26 A C 11: 49,039,101 (GRCm39) N6H possibly damaging Het
Or4c115 G A 2: 88,927,888 (GRCm39) P128S probably damaging Het
Or5d47 A T 2: 87,804,403 (GRCm39) V202D possibly damaging Het
Pabpc4 G T 4: 123,182,861 (GRCm39) R166L possibly damaging Het
Pcdhb8 A G 18: 37,489,015 (GRCm39) E231G possibly damaging Het
Pomgnt2 A T 9: 121,811,257 (GRCm39) I508N possibly damaging Het
Ppp2r5e C G 12: 75,516,341 (GRCm39) A239P probably damaging Het
Prdm15 G T 16: 97,638,885 (GRCm39) D58E probably benign Het
Rgl2 A G 17: 34,151,122 (GRCm39) T117A probably benign Het
Rp1 A G 1: 4,418,943 (GRCm39) I723T probably damaging Het
Serpina3g A G 12: 104,207,536 (GRCm39) E233G probably damaging Het
Skint6 T A 4: 112,749,187 (GRCm39) S798C probably benign Het
Slc35f1 T C 10: 52,898,000 (GRCm39) L137P possibly damaging Het
Slc6a20a T C 9: 123,485,373 (GRCm39) N246S probably damaging Het
Slc7a2 T C 8: 41,369,534 (GRCm39) L663S probably benign Het
Slco1a4 A G 6: 141,761,173 (GRCm39) probably null Het
Slit2 A G 5: 48,439,330 (GRCm39) T41A probably damaging Het
Smc2 T C 4: 52,450,863 (GRCm39) I227T probably damaging Het
Smg1 A G 7: 117,753,422 (GRCm39) probably benign Het
Ssu2 A T 6: 112,361,388 (GRCm39) L23M probably benign Het
Syne2 A G 12: 76,141,053 (GRCm39) probably null Het
Tekt1 T C 11: 72,242,761 (GRCm39) T249A probably benign Het
Tfr2 T C 5: 137,569,954 (GRCm39) V120A probably benign Het
Thsd7b C T 1: 129,605,846 (GRCm39) P529L probably damaging Het
Tll1 C T 8: 64,478,141 (GRCm39) D871N probably damaging Het
Tlr11 A C 14: 50,598,664 (GRCm39) I217L probably benign Het
Tmem87b T A 2: 128,673,479 (GRCm39) V241D probably damaging Het
Tshz2 T A 2: 169,727,465 (GRCm39) I218N possibly damaging Het
Vmn1r18 A T 6: 57,367,026 (GRCm39) I176N possibly damaging Het
Vmn2r68 T A 7: 84,883,260 (GRCm39) H164L probably benign Het
Vmn2r74 T C 7: 85,601,650 (GRCm39) T663A probably benign Het
Vmn2r8 T C 5: 108,945,436 (GRCm39) T724A probably benign Het
Wdr11 T C 7: 129,206,954 (GRCm39) V289A possibly damaging Het
Zfp62 T A 11: 49,107,047 (GRCm39) D379E probably damaging Het
Zfp78 C T 7: 6,381,897 (GRCm39) P316S probably damaging Het
Zfyve27 T G 19: 42,159,987 (GRCm39) M1R probably null Het
Zkscan8 G A 13: 21,709,325 (GRCm39) P191L probably damaging Het
Other mutations in Or13a27
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00225:Or13a27 APN 7 139,925,123 (GRCm39) missense probably damaging 1.00
IGL03493:Or13a27 APN 7 139,925,066 (GRCm39) missense probably damaging 1.00
R0413:Or13a27 UTSW 7 139,925,108 (GRCm39) missense possibly damaging 0.94
R0652:Or13a27 UTSW 7 139,925,545 (GRCm39) missense probably damaging 1.00
R1848:Or13a27 UTSW 7 139,925,900 (GRCm39) start codon destroyed probably benign
R1909:Or13a27 UTSW 7 139,925,378 (GRCm39) missense probably benign 0.05
R5133:Or13a27 UTSW 7 139,925,236 (GRCm39) missense probably damaging 1.00
R5320:Or13a27 UTSW 7 139,925,548 (GRCm39) missense probably benign 0.00
R5429:Or13a27 UTSW 7 139,925,186 (GRCm39) missense possibly damaging 0.67
R6595:Or13a27 UTSW 7 139,925,560 (GRCm39) missense probably damaging 1.00
R6621:Or13a27 UTSW 7 139,925,368 (GRCm39) missense probably damaging 1.00
R7300:Or13a27 UTSW 7 139,925,268 (GRCm39) missense probably damaging 1.00
R7409:Or13a27 UTSW 7 139,925,318 (GRCm39) missense probably benign 0.00
R7562:Or13a27 UTSW 7 139,925,143 (GRCm39) missense probably damaging 1.00
R7646:Or13a27 UTSW 7 139,925,864 (GRCm39) missense probably damaging 1.00
R8158:Or13a27 UTSW 7 139,925,162 (GRCm39) missense probably benign 0.03
R8233:Or13a27 UTSW 7 139,925,411 (GRCm39) missense probably benign 0.01
R8238:Or13a27 UTSW 7 139,925,803 (GRCm39) missense probably damaging 1.00
R8338:Or13a27 UTSW 7 139,925,306 (GRCm39) missense probably benign 0.00
R9794:Or13a27 UTSW 7 139,925,483 (GRCm39) nonsense probably null
Z1088:Or13a27 UTSW 7 139,925,717 (GRCm39) missense probably benign 0.42
Predicted Primers PCR Primer
(F):5'- GAAAAGGCCTTCCTCTTGCC -3'
(R):5'- GGTCCTTGTCTTCAGAGCTG -3'

Sequencing Primer
(F):5'- GCCCTCAGCAGAACGCATG -3'
(R):5'- TGCTGCTCACAGTCATGG -3'
Posted On 2014-06-30