Mutagenetix > Incidental Mutation

Incidental Mutation 'R1911:St6gal1'

210310

Institutional Source

Beutler Lab

Gene Symbol

St6gal1

Ensembl Gene

ENSMUSG00000022885

Gene Name

beta galactoside alpha 2,6 sialyltransferase 1

Synonyms

Siat1, ST6Gal I, St6Gal-I

MMRRC Submission

039929-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.094) question?

Stock #

R1911 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23043490-23179100 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 23140383 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Alanine at position 185 (S185A)

Ref Sequence

ENSEMBL: ENSMUSP00000136206 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023601] [ENSMUST00000115335] [ENSMUST00000128050] [ENSMUST00000178797]

AlphaFold

Q64685

Predicted Effect

probably damaging
Transcript: ENSMUST00000023601
AA Change: S185A

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000023601
Gene: ENSMUSG00000022885
AA Change: S185A

Domain	Start	End	E-Value	Type
transmembrane domain	9	26	N/A	INTRINSIC
Pfam:Glyco_transf_29	127	389	2.3e-63	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000115335
AA Change: S185A

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000110992
Gene: ENSMUSG00000022885
AA Change: S185A

Domain	Start	End	E-Value	Type
transmembrane domain	9	26	N/A	INTRINSIC
Pfam:Glyco_transf_29	140	383	8.3e-36	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000128050

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152449

Predicted Effect

probably damaging
Transcript: ENSMUST00000178797
AA Change: S185A

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000136206
Gene: ENSMUSG00000022885
AA Change: S185A

Domain	Start	End	E-Value	Type
transmembrane domain	9	26	N/A	INTRINSIC
Pfam:Glyco_transf_29	127	389	2.3e-63	PFAM

Meta Mutation Damage Score

0.3432

Coding Region Coverage

1x: 97.3%
3x: 96.8%
10x: 95.4%
20x: 93.2%

Validation Efficiency

99% (99/100)

MGI Phenotype

FUNCTION: This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on chromosome 15. [provided by RefSeq, Nov 2011]
PHENOTYPE: Homozygous mutation of this gene results in altered terminal glycosylation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 96 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930590J08Rik	T	A	6: 91,927,050 (GRCm39)		probably benign	Het
Abca7	T	C	10: 79,842,468 (GRCm39)	V1134A	probably benign	Het
Acaa2	A	G	18: 74,925,483 (GRCm39)	E82G	probably benign	Het
Acap1	T	C	11: 69,772,548 (GRCm39)	D521G	probably damaging	Het
Adam19	T	C	11: 46,012,281 (GRCm39)	V259A	probably damaging	Het
Adss1	T	C	12: 112,599,443 (GRCm39)	V140A	probably benign	Het
Aif1	G	A	17: 35,391,127 (GRCm39)	P44L	probably benign	Het
Aldh3b1	T	G	19: 3,971,187 (GRCm39)	D159A	probably damaging	Het
Ank1	T	C	8: 23,589,666 (GRCm39)	V589A	probably damaging	Het
Ano2	G	A	6: 125,990,654 (GRCm39)	D803N	probably benign	Het
Arid1b	A	G	17: 5,393,241 (GRCm39)	E2257G	probably damaging	Het
Asb17	T	C	3: 153,550,138 (GRCm39)	Y57H	probably benign	Het
Asph	T	C	4: 9,453,335 (GRCm39)	E646G	probably damaging	Het
Aspm	T	A	1: 139,405,832 (GRCm39)	I1573K	probably benign	Het
Bcas1	C	A	2: 170,229,863 (GRCm39)	D236Y	probably damaging	Het
Bcas2	G	T	3: 103,079,113 (GRCm39)	G9*	probably null	Het
Btaf1	A	G	19: 36,964,030 (GRCm39)	Q867R	probably benign	Het
Calhm3	C	T	19: 47,143,908 (GRCm39)	V132I	possibly damaging	Het
Ccer1	A	T	10: 97,530,539 (GRCm39)	I401F	possibly damaging	Het
Cdcp3	T	C	7: 130,839,818 (GRCm39)	V580A	probably damaging	Het
Cecr2	T	A	6: 120,739,526 (GRCm39)		probably benign	Het
Cep104	G	A	4: 154,091,255 (GRCm39)	R925Q	possibly damaging	Het
Cep164	T	A	9: 45,682,104 (GRCm39)	M1900L	probably benign	Het
Crybg1	A	G	10: 43,873,673 (GRCm39)	V1145A	possibly damaging	Het
Cyp2a4	T	A	7: 26,008,399 (GRCm39)	N180K	possibly damaging	Het
Dennd4a	T	C	9: 64,796,368 (GRCm39)	L798P	probably damaging	Het
Dmxl1	T	C	18: 50,011,230 (GRCm39)	I1129T	probably benign	Het
Dnah2	T	C	11: 69,406,578 (GRCm39)	N555D	possibly damaging	Het
Dock1	T	A	7: 134,601,029 (GRCm39)	M988K	probably damaging	Het
Elp4	T	A	2: 105,533,088 (GRCm39)	H419L	probably damaging	Het
Endov	T	C	11: 119,393,177 (GRCm39)	V109A	possibly damaging	Het
Epha8	T	C	4: 136,663,625 (GRCm39)	Y477C	probably damaging	Het
Erlin1	A	G	19: 44,037,561 (GRCm39)	M188T	probably damaging	Het
Fhip1a	T	A	3: 85,568,525 (GRCm39)	D998V	probably benign	Het
Fhod3	A	T	18: 25,245,643 (GRCm39)	D1231V	possibly damaging	Het
Gimap3	T	A	6: 48,742,646 (GRCm39)	I95F	possibly damaging	Het
Gm10717	T	G	9: 3,026,317 (GRCm39)	F205C	probably damaging	Het
Grk1	C	A	8: 13,457,923 (GRCm39)	D274E	probably damaging	Het
Gsdmc2	G	A	15: 63,699,621 (GRCm39)	A269V	probably benign	Het
Krt33a	T	A	11: 99,903,175 (GRCm39)	Q289L	probably benign	Het
Krt76	T	A	15: 101,796,600 (GRCm39)	K403*	probably null	Het
Lcn4	T	C	2: 26,560,607 (GRCm39)		probably benign	Het
Mab21l1	T	C	3: 55,691,048 (GRCm39)	S212P	possibly damaging	Het
Mapk8ip3	A	C	17: 25,123,025 (GRCm39)	D610E	probably benign	Het
Mastl	G	T	2: 23,022,692 (GRCm39)	S677*	probably null	Het
Mfap3	T	C	11: 57,420,562 (GRCm39)	F181S	probably damaging	Het
Mlkl	T	C	8: 112,038,732 (GRCm39)		probably benign	Het
Mov10	G	A	3: 104,708,876 (GRCm39)		probably benign	Het
Muc5ac	T	C	7: 141,350,041 (GRCm39)	F595L	probably benign	Het
Nbas	T	A	12: 13,616,145 (GRCm39)	C2228S	probably benign	Het
Nit2	G	A	16: 56,982,046 (GRCm39)		probably benign	Het
Nod1	C	T	6: 54,921,425 (GRCm39)	V298M	probably damaging	Het
Or3a4	C	A	11: 73,945,210 (GRCm39)	R125L	probably damaging	Het
Or4c111	A	G	2: 88,843,565 (GRCm39)	L281P	probably damaging	Het
Or52b1	T	A	7: 104,978,590 (GRCm39)	I270F	probably benign	Het
Or6c210	A	T	10: 129,495,981 (GRCm39)	D102V	probably benign	Het
Or7g12	T	C	9: 18,900,196 (GRCm39)	L304P	probably damaging	Het
Osbpl5	C	A	7: 143,243,662 (GRCm39)	R864L	probably benign	Het
Pcnt	G	A	10: 76,204,650 (GRCm39)	T2585M	possibly damaging	Het
Pepd	C	T	7: 34,634,174 (GRCm39)		probably benign	Het
Pou6f2	T	C	13: 18,326,548 (GRCm39)	I341V	probably damaging	Het
Pramel32	T	C	4: 88,548,309 (GRCm39)	Q32R	possibly damaging	Het
Prune2	T	A	19: 17,091,038 (GRCm39)	F281I	probably benign	Het
Psg19	T	G	7: 18,528,193 (GRCm39)	Q183H	probably damaging	Het
Psme4	T	G	11: 30,765,658 (GRCm39)	S587A	probably benign	Het
Ptpro	T	C	6: 137,377,617 (GRCm39)		probably benign	Het
Pttg1ip2	C	A	5: 5,502,019 (GRCm39)	W144C	probably benign	Het
Rasgrp4	T	C	7: 28,838,302 (GRCm39)	V92A	probably damaging	Het
Rem1	G	A	2: 152,476,455 (GRCm39)	V238M	probably damaging	Het
Rexo5	C	T	7: 119,398,867 (GRCm39)	A68V	probably damaging	Het
Robo2	A	T	16: 73,755,213 (GRCm39)	N769K	probably damaging	Het
Sfrp5	C	T	19: 42,187,237 (GRCm39)	V278I	probably benign	Het
Sidt1	A	G	16: 44,102,234 (GRCm39)	S309P	possibly damaging	Het
Slc22a6	A	C	19: 8,599,246 (GRCm39)	Q292H	probably benign	Het
Slc4a3	G	A	1: 75,530,367 (GRCm39)	R690H	probably damaging	Het
Snx7	A	T	3: 117,623,317 (GRCm39)		probably null	Het
Spag6	T	A	2: 18,720,616 (GRCm39)	Y129*	probably null	Het
Srcap	T	C	7: 127,133,994 (GRCm39)	I905T	probably damaging	Het
Sult6b1	G	A	17: 79,196,393 (GRCm39)	H250Y	possibly damaging	Het
Tdrd6	T	G	17: 43,937,979 (GRCm39)	N1023T	probably benign	Het
Tecta	C	T	9: 42,249,232 (GRCm39)	E1877K	probably damaging	Het
Tex10	C	T	4: 48,456,800 (GRCm39)	R637Q	probably benign	Het
Tex14	T	A	11: 87,385,861 (GRCm39)	D240E	probably damaging	Het
Tex47	T	C	5: 7,355,022 (GRCm39)	Y68H	probably damaging	Het
Thbs2	A	G	17: 14,910,104 (GRCm39)	V165A	probably benign	Het
Tmem126b	T	A	7: 90,118,367 (GRCm39)	Y171F	possibly damaging	Het
Tpsg1	G	T	17: 25,592,374 (GRCm39)	M46I	probably benign	Het
Trmt2a	A	G	16: 18,069,070 (GRCm39)	K304R	probably benign	Het
Ttc28	G	T	5: 111,428,616 (GRCm39)	R1845L	possibly damaging	Het
Umodl1	A	T	17: 31,211,128 (GRCm39)	T884S	possibly damaging	Het
Vmn2r77	A	G	7: 86,461,001 (GRCm39)	K776E	probably damaging	Het
Vmn2r88	T	C	14: 51,655,671 (GRCm39)	S627P	probably damaging	Het
Vrk1	T	C	12: 106,024,236 (GRCm39)		probably null	Het
Zfp644	A	G	5: 106,783,137 (GRCm39)	M1079T	possibly damaging	Het
Znrf1	T	C	8: 112,348,233 (GRCm39)	*41Q	probably null	Het
Znrf1	T	C	8: 112,348,244 (GRCm39)	F183L	possibly damaging	Het

Other mutations in St6gal1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00426:St6gal1	APN	16	23,175,142 (GRCm39)	splice site	probably benign
IGL01667:St6gal1	APN	16	23,140,174 (GRCm39)	missense	probably benign	0.00
IGL01783:St6gal1	APN	16	23,140,305 (GRCm39)	missense	probably benign	0.29
IGL02996:St6gal1	APN	16	23,139,904 (GRCm39)	missense	probably damaging	0.98
R0049:St6gal1	UTSW	16	23,139,891 (GRCm39)	missense	probably damaging	1.00
R0049:St6gal1	UTSW	16	23,139,891 (GRCm39)	missense	probably damaging	1.00
R0295:St6gal1	UTSW	16	23,174,953 (GRCm39)	splice site	probably benign
R1290:St6gal1	UTSW	16	23,140,411 (GRCm39)	missense	probably benign	0.03
R1352:St6gal1	UTSW	16	23,140,401 (GRCm39)	missense	probably damaging	1.00
R1817:St6gal1	UTSW	16	23,140,083 (GRCm39)	nonsense	probably null
R2113:St6gal1	UTSW	16	23,147,167 (GRCm39)	missense	probably damaging	0.98
R4591:St6gal1	UTSW	16	23,140,044 (GRCm39)	missense	probably benign	0.00
R5761:St6gal1	UTSW	16	23,139,805 (GRCm39)	utr 5 prime	probably benign
R6554:St6gal1	UTSW	16	23,140,405 (GRCm39)	missense	probably benign	0.00
R6925:St6gal1	UTSW	16	23,174,963 (GRCm39)	missense	probably damaging	1.00
R7658:St6gal1	UTSW	16	23,174,978 (GRCm39)	missense	probably damaging	1.00
R7740:St6gal1	UTSW	16	23,139,785 (GRCm39)	splice site	probably benign
R7967:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R7970:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R7973:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R8017:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R8017:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R8018:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R8019:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R8044:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R8122:St6gal1	UTSW	16	23,173,644 (GRCm39)	missense	probably benign	0.00
R8123:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R8124:St6gal1	UTSW	16	23,176,585 (GRCm39)	missense	probably benign	0.01
R9265:St6gal1	UTSW	16	23,140,168 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCAGGAGTCAAGTTCAGCGTAG -3'
(R):5'- ATTCCCCAGTCAATCCTCGGAC -3'

Sequencing Primer
(F):5'- TCAAGTTCAGCGTAGAGGCG -3'
(R):5'- GAAATGTCTCCATGAGATCCAGCTG -3'

Posted On

2014-06-30