Incidental Mutation 'R1871:Wif1'
ID210758
Institutional Source Beutler Lab
Gene Symbol Wif1
Ensembl Gene ENSMUSG00000020218
Gene NameWnt inhibitory factor 1
SynonymsWIF-1
MMRRC Submission 039893-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R1871 (G1)
Quality Score225
Status Validated
Chromosome10
Chromosomal Location121033960-121100650 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 121084919 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Methionine at position 215 (I215M)
Ref Sequence ENSEMBL: ENSMUSP00000135486 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020439] [ENSMUST00000175867]
Predicted Effect probably benign
Transcript: ENSMUST00000020439
AA Change: I215M

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000020439
Gene: ENSMUSG00000020218
AA Change: I215M

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WIF 35 179 8.47e-90 SMART
EGF 181 210 3.88e-3 SMART
EGF 213 242 4.46e-3 SMART
EGF 245 274 4.7e-2 SMART
EGF 277 306 1.69e-3 SMART
EGF 309 338 7.95e0 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000145691
Predicted Effect probably benign
Transcript: ENSMUST00000175867
AA Change: I215M

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000135486
Gene: ENSMUSG00000020218
AA Change: I215M

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WIF 35 179 8.47e-90 SMART
EGF 181 210 3.88e-3 SMART
EGF 213 242 4.46e-3 SMART
EGF 245 274 4.7e-2 SMART
EGF 295 324 7.95e0 SMART
Meta Mutation Damage Score 0.1204 question?
Coding Region Coverage
  • 1x: 97.5%
  • 3x: 97.0%
  • 10x: 95.6%
  • 20x: 93.2%
Validation Efficiency 97% (87/90)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
PHENOTYPE: Homozygous null mice are viable and fertile but display increased susceptibility to spontaneous and induced osteosarcomas. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 80 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9330182L06Rik A G 5: 9,418,007 E225G probably damaging Het
A930009A15Rik A G 10: 115,579,794 probably null Het
Abca13 A T 11: 9,292,134 E1332D probably benign Het
Abce1 G A 8: 79,685,268 Q588* probably null Het
Acads A T 5: 115,117,642 C45S probably damaging Het
Adamts12 T C 15: 11,311,154 S1166P probably benign Het
Ano4 T A 10: 88,993,027 I517F probably damaging Het
Arpc1a T A 5: 145,107,091 C344S possibly damaging Het
Blk A T 14: 63,375,915 S381R possibly damaging Het
Capn15 C A 17: 25,964,229 W426L probably damaging Het
Chd6 T C 2: 160,990,256 T999A probably damaging Het
Clca3a2 A G 3: 144,797,637 Y851H probably benign Het
Crp T A 1: 172,698,605 W86R possibly damaging Het
Ctnnal1 G A 4: 56,812,534 L705F probably benign Het
Cyp2c66 A G 19: 39,163,414 D191G possibly damaging Het
Cyp4f37 A G 17: 32,634,665 D441G probably damaging Het
D130040H23Rik T A 8: 69,302,702 I253N probably benign Het
Dnah5 T A 15: 28,331,713 Y2148* probably null Het
Eef1g A T 19: 8,977,966 D393V possibly damaging Het
Ephx2 A T 14: 66,084,734 I538N probably damaging Het
Fat4 T A 3: 38,981,072 S2958T possibly damaging Het
Foxi1 A T 11: 34,207,937 N29K possibly damaging Het
Gm10287 G T 3: 149,224,737 noncoding transcript Het
Gm10801 G T 2: 98,663,840 S109I probably benign Het
Gm5346 A T 8: 43,625,095 Y697* probably null Het
Gmcl1 T C 6: 86,697,516 D460G probably benign Het
Gpr158 A G 2: 21,815,615 D641G probably damaging Het
Heph C A X: 96,499,084 S561Y probably benign Het
Hgfac A G 5: 35,042,913 *90W probably null Het
Hp1bp3 C G 4: 138,222,186 P65R probably damaging Het
Ighv6-4 T A 12: 114,406,601 Y58F probably benign Het
Jrk T C 15: 74,706,563 D291G possibly damaging Het
Kdm4d A G 9: 14,464,383 Y60H probably damaging Het
Khdrbs3 T C 15: 69,049,442 Y203H probably damaging Het
Klhl6 A T 16: 19,957,043 V255D possibly damaging Het
Krt9 C T 11: 100,190,788 R305H probably damaging Het
Lama2 T G 10: 26,984,494 N2999T probably damaging Het
Letm2 A G 8: 25,596,444 probably benign Het
Lipa A T 19: 34,510,928 L106Q probably damaging Het
Llph A T 10: 120,231,236 N86I probably damaging Het
Lyst T A 13: 13,651,712 N1601K probably benign Het
Mc1r T A 8: 123,407,536 S9R probably benign Het
Mfsd14a A G 3: 116,641,320 I249T probably benign Het
Mtfp1 G A 11: 4,094,012 R73C probably benign Het
Myo1a A G 10: 127,719,671 Q877R probably benign Het
Nalcn A C 14: 123,594,553 V103G possibly damaging Het
Ndst3 A T 3: 123,562,024 F119I probably damaging Het
Olfr1008 A G 2: 85,690,311 N294S probably damaging Het
Patl1 T C 19: 11,925,232 probably benign Het
Pcdhb2 A T 18: 37,297,355 probably null Het
Pcdhb22 A C 18: 37,519,147 T223P probably damaging Het
Pcdhga1 T A 18: 37,840,090 N907K probably damaging Het
Pfas T C 11: 68,991,969 D782G probably damaging Het
Plpp6 T C 19: 28,964,284 F95S probably damaging Het
Polr2b T A 5: 77,326,527 probably benign Het
Qars T G 9: 108,514,116 probably null Het
Ranbp2 A G 10: 58,492,561 I2800V probably damaging Het
Rnf213 T C 11: 119,450,129 V3532A probably benign Het
Rubcnl T C 14: 75,042,409 S411P possibly damaging Het
Scn1a C A 2: 66,318,025 G1059W probably damaging Het
Sgms1 C T 19: 32,159,882 V95I probably benign Het
Siglecf A G 7: 43,355,543 N399S probably benign Het
Slc29a4 T C 5: 142,721,488 *529R probably null Het
Slfn9 G T 11: 82,981,576 T778N probably benign Het
Sorl1 G A 9: 41,969,725 Q2167* probably null Het
Stkld1 A G 2: 26,937,973 probably benign Het
Taar8b T C 10: 24,092,002 Y98C probably damaging Het
Tecta G A 9: 42,337,176 L1977F probably damaging Het
Tecta G C 9: 42,337,340 T1917R probably damaging Het
Topaz1 T A 9: 122,799,479 S1544T probably benign Het
Tpx2 T C 2: 152,893,603 I717T probably damaging Het
Tyk2 A T 9: 21,121,441 V342E probably damaging Het
Ube4a A T 9: 44,944,937 probably null Het
Unc80 A T 1: 66,510,717 R711S possibly damaging Het
Ush2a C A 1: 188,826,468 D3631E probably benign Het
Usp34 A G 11: 23,364,479 H807R probably benign Het
Vps13a T C 19: 16,664,664 T2200A probably benign Het
Wnk1 T C 6: 119,951,089 T1134A probably damaging Het
Xxylt1 A G 16: 30,957,417 V367A probably damaging Het
Zfp735 A T 11: 73,710,586 K119* probably null Het
Other mutations in Wif1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01384:Wif1 APN 10 121084950 missense possibly damaging 0.55
IGL01707:Wif1 APN 10 121083985 critical splice donor site probably null
IGL01932:Wif1 APN 10 121096035 missense probably damaging 1.00
IGL02183:Wif1 APN 10 121075276 missense probably damaging 1.00
R0653:Wif1 UTSW 10 121099799 missense probably benign 0.01
R1858:Wif1 UTSW 10 121083883 splice site probably null
R1869:Wif1 UTSW 10 121084919 missense probably benign 0.00
R4056:Wif1 UTSW 10 121082194 missense probably benign 0.04
R4057:Wif1 UTSW 10 121082194 missense probably benign 0.04
R5056:Wif1 UTSW 10 121099779 missense probably benign 0.00
R6242:Wif1 UTSW 10 121034461 missense possibly damaging 0.82
R6504:Wif1 UTSW 10 121035091 missense probably damaging 0.96
R7220:Wif1 UTSW 10 121090114 missense possibly damaging 0.76
Predicted Primers PCR Primer
(F):5'- GCCTGAGAAATAACCGGGTTAATG -3'
(R):5'- CTTCACCTCACAGGATACACTG -3'

Sequencing Primer
(F):5'- TAACCGGGTTAATGTCACAAAAC -3'
(R):5'- ACCATTTGTAAATTCAGGTCCTGGG -3'
Posted On2014-06-30