Mutagenetix > Incidental Mutation

Incidental Mutation 'R1875:Fry'

211134

Institutional Source

Beutler Lab

Gene Symbol

Fry

Ensembl Gene

ENSMUSG00000056602

Gene Name

FRY microtubule binding protein

Synonyms

cg003, 9330186A19Rik

MMRRC Submission

039897-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.469) question?

Stock #

R1875 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

150042110-150421218 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 150249597 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 136 (E136G)

Ref Sequence

ENSEMBL: ENSMUSP00000144317 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087204] [ENSMUST00000200960] [ENSMUST00000202530] [ENSMUST00000202600]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000087204
AA Change: E91G

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000084454
Gene: ENSMUSG00000056602
AA Change: E91G

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	165	697	5.3e-170	PFAM
low complexity region	1014	1040	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1188	1380	1.2e-15	PFAM
Pfam:MOR2-PAG1_mid	1398	1534	1.5e-5	PFAM
Pfam:MOR2-PAG1_mid	1632	1704	1.8e-7	PFAM
Pfam:MOR2-PAG1_mid	1772	1906	4.9e-10	PFAM
low complexity region	1936	1956	N/A	INTRINSIC
low complexity region	1962	1980	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2050	2303	1.9e-74	PFAM
low complexity region	2369	2385	N/A	INTRINSIC
low complexity region	2463	2482	N/A	INTRINSIC
low complexity region	2525	2534	N/A	INTRINSIC
low complexity region	2836	2852	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000200960
AA Change: E41G

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Predicted Effect

probably damaging
Transcript: ENSMUST00000202530
AA Change: E41G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000144277
Gene: ENSMUSG00000056602
AA Change: E41G

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	115	159	3.3e-7	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000202600
AA Change: E136G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000144317
Gene: ENSMUSG00000056602
AA Change: E136G

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000202841

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203000

Meta Mutation Damage Score

0.2561

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 92.1%

Validation Efficiency

98% (85/87)

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	A	T	7: 119,847,190 (GRCm39)	M685L	possibly damaging	Het
Abi3bp	A	G	16: 56,394,862 (GRCm39)	Y190C	probably damaging	Het
Adam26a	C	A	8: 44,022,888 (GRCm39)	V201L	probably benign	Het
Adamts20	G	T	15: 94,229,277 (GRCm39)	D947E	probably benign	Het
Ankrd26	A	G	6: 118,517,410 (GRCm39)		probably null	Het
Apol11a	C	A	15: 77,397,766 (GRCm39)	T39N	possibly damaging	Het
Arhgef38	T	A	3: 132,839,501 (GRCm39)		probably null	Het
Armh4	T	C	14: 49,919,815 (GRCm39)	D772G	probably damaging	Het
Atp11b	T	C	3: 35,893,296 (GRCm39)	L883P	probably damaging	Het
Btnl10	T	A	11: 58,814,586 (GRCm39)	I422N	probably damaging	Het
C2cd3	A	G	7: 100,056,232 (GRCm39)	K547E	possibly damaging	Het
Cdh2	T	A	18: 16,757,934 (GRCm39)	L549F	probably benign	Het
Celsr3	T	C	9: 108,713,037 (GRCm39)	V1825A	probably benign	Het
Cfap221	T	C	1: 119,881,389 (GRCm39)	I358V	probably benign	Het
Cimap2	G	A	4: 106,470,453 (GRCm39)		probably benign	Het
Csmd1	T	C	8: 15,979,101 (GRCm39)	K2828E	probably damaging	Het
Ddah2	T	C	17: 35,279,821 (GRCm39)	F137S	probably damaging	Het
Ddx21	A	G	10: 62,429,847 (GRCm39)	I299T	probably damaging	Het
Dnah7a	A	T	1: 53,495,691 (GRCm39)		probably benign	Het
Elmod1	A	G	9: 53,843,151 (GRCm39)	I9T	probably benign	Het
Epha2	A	G	4: 141,036,290 (GRCm39)	E242G	probably benign	Het
Erbb3	T	C	10: 128,410,335 (GRCm39)	H641R	possibly damaging	Het
Fbxl18	G	A	5: 142,871,978 (GRCm39)	A419V	probably damaging	Het
Fli1	T	C	9: 32,335,209 (GRCm39)	M408V	probably benign	Het
Fmo4	T	C	1: 162,631,187 (GRCm39)	N260S	possibly damaging	Het
Gm10477	T	A	X: 55,570,127 (GRCm39)	F9Y	probably damaging	Het
Gm8258	A	G	5: 104,924,320 (GRCm39)		noncoding transcript	Het
Gpr68	T	A	12: 100,845,049 (GRCm39)	D165V	probably damaging	Het
Htt	T	A	5: 34,951,456 (GRCm39)	M139K	probably benign	Het
Jup	A	G	11: 100,263,120 (GRCm39)		probably null	Het
Kifc5b	G	A	17: 27,136,264 (GRCm39)		probably null	Het
Krba1	T	A	6: 48,390,983 (GRCm39)		probably null	Het
Lamp1	G	A	8: 13,217,257 (GRCm39)	G89R	probably damaging	Het
Lrrc17	C	T	5: 21,765,650 (GRCm39)	S44F	possibly damaging	Het
Mdga1	T	C	17: 30,071,581 (GRCm39)	T347A	probably damaging	Het
Mical3	A	T	6: 121,019,025 (GRCm39)	W66R	probably damaging	Het
Mpl	A	G	4: 118,314,026 (GRCm39)	Y73H	probably benign	Het
Mterf1b	T	A	5: 4,247,364 (GRCm39)	I335N	possibly damaging	Het
Mylk3	A	G	8: 86,079,494 (GRCm39)	I388T	probably damaging	Het
Myo15a	C	T	11: 60,398,354 (GRCm39)	R2775W	probably damaging	Het
Myoz2	A	T	3: 122,819,765 (GRCm39)	S65T	probably damaging	Het
Ndrg1	T	C	15: 66,802,940 (GRCm39)	T137A	possibly damaging	Het
Neil3	G	T	8: 54,052,454 (GRCm39)	N381K	probably damaging	Het
Obsl1	T	C	1: 75,474,877 (GRCm39)	Y841C	probably damaging	Het
Or2b4	A	G	17: 38,115,996 (GRCm39)		probably benign	Het
Or6b6	G	A	7: 106,571,389 (GRCm39)	S54F	possibly damaging	Het
Otogl	T	C	10: 107,735,451 (GRCm39)	D111G	probably damaging	Het
Parp10	T	C	15: 76,127,051 (GRCm39)	E103G	probably damaging	Het
Pars2	T	C	4: 106,510,913 (GRCm39)	F232L	possibly damaging	Het
Pcdh18	A	T	3: 49,709,154 (GRCm39)	F720L	probably damaging	Het
Phf3	T	C	1: 30,869,704 (GRCm39)	E448G	possibly damaging	Het
Pigc	A	G	1: 161,798,516 (GRCm39)	Y166C	probably damaging	Het
Pik3c2a	A	T	7: 116,017,206 (GRCm39)	S184T	probably benign	Het
Pkd1l1	A	G	11: 8,794,670 (GRCm39)		probably benign	Het
Plch2	G	A	4: 155,082,965 (GRCm39)	S485F	probably damaging	Het
Plxnd1	G	T	6: 115,955,045 (GRCm39)		probably null	Het
Pnliprp2	G	T	19: 58,751,821 (GRCm39)	V189L	probably benign	Het
Prl8a2	T	C	13: 27,535,037 (GRCm39)	V103A	probably benign	Het
Psg23	G	A	7: 18,344,375 (GRCm39)	T360I	probably benign	Het
Rad51c	A	T	11: 87,279,469 (GRCm39)	I323N	probably damaging	Het
Rsbn1l	C	T	5: 21,156,696 (GRCm39)	E30K	probably benign	Het
Serpina3c	C	A	12: 104,118,145 (GRCm39)	L64F	probably damaging	Het
Shroom1	A	G	11: 53,356,502 (GRCm39)	D392G	probably damaging	Het
Slc26a5	T	A	5: 22,020,725 (GRCm39)	I540L	probably benign	Het
Slc37a4	A	G	9: 44,312,808 (GRCm39)	T321A	probably damaging	Het
Slc41a2	A	G	10: 83,091,949 (GRCm39)	L438S	probably damaging	Het
Spef2	C	T	15: 9,584,194 (GRCm39)	E1624K	probably damaging	Het
Spef2	C	T	15: 9,597,487 (GRCm39)	G1390R	possibly damaging	Het
Spmap2l	A	T	5: 77,202,431 (GRCm39)	K284M	probably benign	Het
Synj2	G	T	17: 6,078,825 (GRCm39)	A740S	possibly damaging	Het
Tigd4	A	C	3: 84,502,394 (GRCm39)	D437A	probably benign	Het
Timm21	G	C	18: 84,967,387 (GRCm39)	L130V	probably damaging	Het
Tmem106a	CAGCTCAACACGACGGTA	CAGCTCAACACGACGGTAAGCTCAACACGACGGTA	11: 101,477,204 (GRCm39)		probably benign	Het
Tmem131l	A	T	3: 83,812,383 (GRCm39)	C1313*	probably null	Het
Tmem86b	A	T	7: 4,632,698 (GRCm39)	I47N	possibly damaging	Het
Tspan13	T	C	12: 36,070,550 (GRCm39)		probably null	Het
Vps54	A	G	11: 21,250,251 (GRCm39)	T396A	probably benign	Het
Zfp106	A	T	2: 120,344,096 (GRCm39)		probably null	Het
Zfp668	T	C	7: 127,465,654 (GRCm39)		probably null	Het
Zfp809	A	T	9: 22,150,027 (GRCm39)	R175*	probably null	Het

Other mutations in Fry

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00327:Fry	APN	5	150,263,869 (GRCm39)	nonsense	probably null
IGL00328:Fry	APN	5	150,263,869 (GRCm39)	nonsense	probably null
IGL00841:Fry	APN	5	150,346,189 (GRCm39)	missense	probably benign
IGL00938:Fry	APN	5	150,293,645 (GRCm39)	missense	probably damaging	1.00
IGL01015:Fry	APN	5	150,346,252 (GRCm39)	missense	probably benign	0.18
IGL01401:Fry	APN	5	150,362,253 (GRCm39)	missense	probably benign
IGL01616:Fry	APN	5	150,323,064 (GRCm39)	missense	probably damaging	1.00
IGL01616:Fry	APN	5	150,362,276 (GRCm39)	splice site	probably null
IGL01748:Fry	APN	5	150,269,116 (GRCm39)	splice site	probably benign
IGL01965:Fry	APN	5	150,305,086 (GRCm39)	missense	probably damaging	1.00
IGL02030:Fry	APN	5	150,395,083 (GRCm39)	splice site	probably benign
IGL02079:Fry	APN	5	150,323,089 (GRCm39)	missense	probably damaging	0.97
IGL02087:Fry	APN	5	150,327,059 (GRCm39)	missense	probably benign	0.23
IGL02113:Fry	APN	5	150,323,070 (GRCm39)	missense	probably benign
IGL02209:Fry	APN	5	150,360,491 (GRCm39)	missense	probably benign	0.00
IGL02250:Fry	APN	5	150,326,899 (GRCm39)	splice site	probably benign
IGL02265:Fry	APN	5	150,360,618 (GRCm39)	missense	probably damaging	1.00
IGL02486:Fry	APN	5	150,414,642 (GRCm39)	missense	probably damaging	0.99
IGL02552:Fry	APN	5	150,304,375 (GRCm39)	missense	probably damaging	1.00
IGL02881:Fry	APN	5	150,282,516 (GRCm39)	missense	probably damaging	0.99
IGL03008:Fry	APN	5	150,269,021 (GRCm39)	missense	possibly damaging	0.82
IGL03140:Fry	APN	5	150,419,166 (GRCm39)	missense	probably damaging	0.98
IGL03171:Fry	APN	5	150,304,274 (GRCm39)	missense	probably damaging	1.00
IGL03389:Fry	APN	5	150,317,696 (GRCm39)	missense	probably damaging	1.00
IGL03404:Fry	APN	5	150,249,633 (GRCm39)	missense	probably damaging	1.00
Brook	UTSW	5	150,249,597 (GRCm39)	missense	probably damaging	1.00
haydn	UTSW	5	150,341,929 (GRCm39)	missense	possibly damaging	0.94
miracle	UTSW	5	150,360,624 (GRCm39)	missense	probably damaging	0.99
quickening	UTSW	5	150,358,241 (GRCm39)	missense	probably damaging	1.00
seasons	UTSW	5	150,389,902 (GRCm39)	missense	probably benign	0.06
Vivaldi	UTSW	5	150,317,603 (GRCm39)	missense	possibly damaging	0.80
R0023:Fry	UTSW	5	150,374,563 (GRCm39)	missense	possibly damaging	0.78
R0024:Fry	UTSW	5	150,304,268 (GRCm39)	missense	probably benign	0.03
R0030:Fry	UTSW	5	150,296,034 (GRCm39)	nonsense	probably null
R0053:Fry	UTSW	5	150,384,842 (GRCm39)	splice site	probably benign
R0089:Fry	UTSW	5	150,263,892 (GRCm39)	missense	possibly damaging	0.91
R0212:Fry	UTSW	5	150,419,862 (GRCm39)	missense	probably damaging	0.99
R0241:Fry	UTSW	5	150,183,811 (GRCm39)	intron	probably benign
R0265:Fry	UTSW	5	150,358,241 (GRCm39)	missense	probably damaging	1.00
R0317:Fry	UTSW	5	150,394,933 (GRCm39)	missense	probably damaging	1.00
R0532:Fry	UTSW	5	150,402,226 (GRCm39)	splice site	probably benign
R0532:Fry	UTSW	5	150,357,172 (GRCm39)	unclassified	probably benign
R0599:Fry	UTSW	5	150,360,624 (GRCm39)	missense	probably damaging	0.99
R0631:Fry	UTSW	5	150,419,817 (GRCm39)	missense	possibly damaging	0.82
R0723:Fry	UTSW	5	150,419,825 (GRCm39)	missense	probably damaging	1.00
R0766:Fry	UTSW	5	150,326,897 (GRCm39)	splice site	probably benign
R0790:Fry	UTSW	5	150,389,902 (GRCm39)	missense	probably benign	0.06
R0928:Fry	UTSW	5	150,360,549 (GRCm39)	missense	probably damaging	1.00
R1104:Fry	UTSW	5	150,419,754 (GRCm39)	missense	probably damaging	1.00
R1144:Fry	UTSW	5	150,341,929 (GRCm39)	missense	possibly damaging	0.94
R1172:Fry	UTSW	5	150,404,959 (GRCm39)	nonsense	probably null
R1312:Fry	UTSW	5	150,326,897 (GRCm39)	splice site	probably benign
R1347:Fry	UTSW	5	150,419,283 (GRCm39)	missense	probably damaging	1.00
R1347:Fry	UTSW	5	150,419,283 (GRCm39)	missense	probably damaging	1.00
R1437:Fry	UTSW	5	150,233,890 (GRCm39)	missense	possibly damaging	0.92
R1458:Fry	UTSW	5	150,304,324 (GRCm39)	missense	probably damaging	1.00
R1542:Fry	UTSW	5	150,328,431 (GRCm39)	missense	probably benign	0.13
R1692:Fry	UTSW	5	150,293,692 (GRCm39)	missense	probably damaging	1.00
R1826:Fry	UTSW	5	150,360,174 (GRCm39)	missense	possibly damaging	0.82
R1874:Fry	UTSW	5	150,269,386 (GRCm39)	missense	probably damaging	1.00
R1881:Fry	UTSW	5	150,401,511 (GRCm39)	missense	probably damaging	0.97
R1884:Fry	UTSW	5	150,326,985 (GRCm39)	missense	probably benign	0.00
R1929:Fry	UTSW	5	150,324,389 (GRCm39)	missense	probably null	0.02
R2066:Fry	UTSW	5	150,293,584 (GRCm39)	splice site	probably benign
R2270:Fry	UTSW	5	150,324,389 (GRCm39)	missense	probably null	0.02
R2356:Fry	UTSW	5	150,394,897 (GRCm39)	missense	probably benign
R3720:Fry	UTSW	5	150,378,037 (GRCm39)	missense	probably damaging	1.00
R3773:Fry	UTSW	5	150,321,663 (GRCm39)	missense	probably damaging	0.96
R3824:Fry	UTSW	5	150,419,884 (GRCm39)	missense	possibly damaging	0.94
R3902:Fry	UTSW	5	150,269,392 (GRCm39)	missense	probably damaging	1.00
R3923:Fry	UTSW	5	150,336,814 (GRCm39)	missense	probably benign
R4250:Fry	UTSW	5	150,233,825 (GRCm39)	missense	probably damaging	0.99
R4332:Fry	UTSW	5	150,305,128 (GRCm39)	missense	probably damaging	1.00
R4495:Fry	UTSW	5	150,233,928 (GRCm39)	missense	probably damaging	1.00
R4610:Fry	UTSW	5	150,309,569 (GRCm39)	missense	probably damaging	1.00
R4682:Fry	UTSW	5	150,346,219 (GRCm39)	missense	probably damaging	1.00
R4732:Fry	UTSW	5	150,309,472 (GRCm39)	missense
R4733:Fry	UTSW	5	150,309,472 (GRCm39)	missense
R4755:Fry	UTSW	5	150,321,719 (GRCm39)	missense	probably damaging	0.99
R4788:Fry	UTSW	5	150,323,101 (GRCm39)	missense	probably benign	0.00
R4803:Fry	UTSW	5	150,322,998 (GRCm39)	missense	probably benign	0.31
R4858:Fry	UTSW	5	150,325,108 (GRCm39)	missense	possibly damaging	0.78
R4872:Fry	UTSW	5	150,317,704 (GRCm39)	critical splice donor site	probably null
R4902:Fry	UTSW	5	150,419,168 (GRCm39)	missense	probably benign	0.43
R4915:Fry	UTSW	5	150,402,328 (GRCm39)	missense	probably benign	0.30
R4938:Fry	UTSW	5	150,401,454 (GRCm39)	missense	probably damaging	1.00
R4983:Fry	UTSW	5	150,321,719 (GRCm39)	missense	probably damaging	1.00
R5004:Fry	UTSW	5	150,357,069 (GRCm39)	missense	probably benign	0.16
R5040:Fry	UTSW	5	150,312,319 (GRCm39)	missense	probably damaging	0.99
R5145:Fry	UTSW	5	150,293,689 (GRCm39)	missense	probably damaging	0.98
R5170:Fry	UTSW	5	150,353,319 (GRCm39)	missense	probably benign	0.03
R5233:Fry	UTSW	5	150,393,185 (GRCm39)	missense	possibly damaging	0.71
R5428:Fry	UTSW	5	150,328,824 (GRCm39)	missense	possibly damaging	0.89
R5468:Fry	UTSW	5	150,323,053 (GRCm39)	missense	probably benign	0.44
R5481:Fry	UTSW	5	150,183,784 (GRCm39)	missense	probably benign	0.01
R5494:Fry	UTSW	5	150,314,132 (GRCm39)	missense	probably damaging	1.00
R5538:Fry	UTSW	5	150,419,313 (GRCm39)	missense	probably damaging	1.00
R5638:Fry	UTSW	5	150,282,546 (GRCm39)	missense	possibly damaging	0.46
R5645:Fry	UTSW	5	150,304,332 (GRCm39)	missense	probably damaging	1.00
R5716:Fry	UTSW	5	150,293,686 (GRCm39)	nonsense	probably null
R5812:Fry	UTSW	5	150,323,136 (GRCm39)	missense	probably damaging	0.99
R5813:Fry	UTSW	5	150,323,136 (GRCm39)	missense	probably damaging	0.99
R5873:Fry	UTSW	5	150,302,350 (GRCm39)	missense	probably damaging	1.00
R5933:Fry	UTSW	5	150,314,265 (GRCm39)	intron	probably benign
R6037:Fry	UTSW	5	150,351,644 (GRCm39)	missense	probably benign	0.03
R6037:Fry	UTSW	5	150,351,644 (GRCm39)	missense	probably benign	0.03
R6158:Fry	UTSW	5	150,378,037 (GRCm39)	missense	probably damaging	1.00
R6178:Fry	UTSW	5	150,377,987 (GRCm39)	missense	probably damaging	1.00
R6481:Fry	UTSW	5	150,309,479 (GRCm39)	missense	probably damaging	1.00
R6562:Fry	UTSW	5	150,249,614 (GRCm39)	missense	probably damaging	1.00
R6676:Fry	UTSW	5	150,304,387 (GRCm39)	missense	probably benign	0.22
R6717:Fry	UTSW	5	150,419,777 (GRCm39)	missense	probably benign	0.00
R6828:Fry	UTSW	5	150,389,911 (GRCm39)	splice site	probably null
R6874:Fry	UTSW	5	150,360,768 (GRCm39)	missense	probably benign	0.00
R6930:Fry	UTSW	5	150,351,695 (GRCm39)	missense	probably benign	0.00
R6963:Fry	UTSW	5	150,381,309 (GRCm39)	missense	probably benign	0.17
R6965:Fry	UTSW	5	150,339,685 (GRCm39)	missense	possibly damaging	0.79
R7051:Fry	UTSW	5	150,318,634 (GRCm39)	missense	possibly damaging	0.93
R7085:Fry	UTSW	5	150,362,214 (GRCm39)	missense	probably benign	0.02
R7108:Fry	UTSW	5	150,414,555 (GRCm39)	missense
R7108:Fry	UTSW	5	150,319,251 (GRCm39)	missense	probably damaging	1.00
R7115:Fry	UTSW	5	150,309,532 (GRCm39)	missense	probably damaging	1.00
R7116:Fry	UTSW	5	150,319,334 (GRCm39)	critical splice donor site	probably null
R7197:Fry	UTSW	5	150,393,232 (GRCm39)	missense
R7256:Fry	UTSW	5	150,390,251 (GRCm39)	missense
R7318:Fry	UTSW	5	150,360,458 (GRCm39)	missense	probably damaging	0.98
R7323:Fry	UTSW	5	150,419,814 (GRCm39)	missense
R7358:Fry	UTSW	5	150,339,788 (GRCm39)	missense	probably benign
R7361:Fry	UTSW	5	150,360,312 (GRCm39)	missense	possibly damaging	0.92
R7395:Fry	UTSW	5	150,304,348 (GRCm39)	missense	possibly damaging	0.82
R7487:Fry	UTSW	5	150,338,039 (GRCm39)	missense	possibly damaging	0.79
R7491:Fry	UTSW	5	150,389,791 (GRCm39)	missense
R7574:Fry	UTSW	5	150,304,359 (GRCm39)	missense	probably benign	0.00
R7582:Fry	UTSW	5	150,419,847 (GRCm39)	missense
R7586:Fry	UTSW	5	150,349,683 (GRCm39)	missense	probably damaging	1.00
R7650:Fry	UTSW	5	150,336,883 (GRCm39)	missense	probably damaging	1.00
R7699:Fry	UTSW	5	150,328,792 (GRCm39)	missense	probably damaging	0.98
R7700:Fry	UTSW	5	150,328,792 (GRCm39)	missense	probably damaging	0.98
R7972:Fry	UTSW	5	150,233,861 (GRCm39)	missense	probably benign	0.05
R8058:Fry	UTSW	5	150,419,232 (GRCm39)	missense
R8070:Fry	UTSW	5	150,401,472 (GRCm39)	missense
R8159:Fry	UTSW	5	150,322,998 (GRCm39)	missense	probably benign	0.31
R8202:Fry	UTSW	5	150,355,202 (GRCm39)	missense	probably damaging	1.00
R8261:Fry	UTSW	5	150,369,372 (GRCm39)	missense	probably damaging	1.00
R8279:Fry	UTSW	5	150,419,726 (GRCm39)	missense
R8338:Fry	UTSW	5	150,282,516 (GRCm39)	missense	probably damaging	0.99
R8370:Fry	UTSW	5	150,319,284 (GRCm39)	missense	probably damaging	1.00
R8673:Fry	UTSW	5	150,318,576 (GRCm39)	missense	possibly damaging	0.91
R8786:Fry	UTSW	5	150,317,501 (GRCm39)	missense	probably benign	0.00
R8815:Fry	UTSW	5	150,317,603 (GRCm39)	missense	possibly damaging	0.80
R8847:Fry	UTSW	5	150,309,472 (GRCm39)	missense
R9023:Fry	UTSW	5	150,360,768 (GRCm39)	missense	probably benign	0.00
R9025:Fry	UTSW	5	150,219,273 (GRCm39)	intron	probably benign
R9125:Fry	UTSW	5	150,269,525 (GRCm39)	missense	probably damaging	0.97
R9172:Fry	UTSW	5	150,336,793 (GRCm39)	missense	probably benign
R9262:Fry	UTSW	5	150,305,109 (GRCm39)	missense	probably damaging	1.00
R9263:Fry	UTSW	5	150,322,728 (GRCm39)	missense	probably damaging	1.00
R9293:Fry	UTSW	5	150,419,297 (GRCm39)	missense
R9368:Fry	UTSW	5	150,401,403 (GRCm39)	missense
R9401:Fry	UTSW	5	150,302,403 (GRCm39)	missense	probably damaging	1.00
R9402:Fry	UTSW	5	150,360,318 (GRCm39)	missense	probably damaging	1.00
R9402:Fry	UTSW	5	150,357,161 (GRCm39)	missense	possibly damaging	0.91
R9420:Fry	UTSW	5	150,356,994 (GRCm39)	missense	possibly damaging	0.72
R9557:Fry	UTSW	5	150,389,781 (GRCm39)	missense
R9647:Fry	UTSW	5	150,292,984 (GRCm39)	missense	probably damaging	1.00
R9650:Fry	UTSW	5	150,369,375 (GRCm39)	missense	probably damaging	1.00
R9655:Fry	UTSW	5	150,362,251 (GRCm39)	missense	possibly damaging	0.90
R9664:Fry	UTSW	5	150,282,488 (GRCm39)	missense	probably damaging	0.98
R9668:Fry	UTSW	5	150,282,318 (GRCm39)	missense	probably damaging	1.00
R9732:Fry	UTSW	5	150,328,758 (GRCm39)	missense	probably benign	0.00
R9773:Fry	UTSW	5	150,322,728 (GRCm39)	missense	probably damaging	1.00
Z1177:Fry	UTSW	5	150,233,902 (GRCm39)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- AATTCCTAAGTCTCCCAGGGG -3'
(R):5'- TCTGACGTTCCATGTAGCTC -3'

Sequencing Primer
(F):5'- CCCCTCGTTGTATGTAGG -3'
(R):5'- CATGTCAAACACCAGGGCCTTG -3'

Posted On

2014-06-30