Mutagenetix > Incidental Mutation

Incidental Mutation 'R1924:Cacna1g'

213296

Institutional Source

Beutler Lab

Gene Symbol

Cacna1g

Ensembl Gene

ENSMUSG00000020866

Gene Name

calcium channel, voltage-dependent, T type, alpha 1G subunit

Synonyms

a1G, Cav3.1d

MMRRC Submission

039942-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.339) question?

Stock #

R1924 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

94299217-94365024 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 94334880 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 809 (I809T)

Ref Sequence

ENSEMBL: ENSMUSP00000103419 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021234] [ENSMUST00000100561] [ENSMUST00000103166] [ENSMUST00000107785] [ENSMUST00000107786] [ENSMUST00000107788] [ENSMUST00000107789] [ENSMUST00000107790] [ENSMUST00000107793] [ENSMUST00000107791] [ENSMUST00000107792]

AlphaFold

Q5SUF7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000021234
AA Change: I809T

PolyPhen 2 Score 0.931 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000021234
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	9.2e-66	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	7.9e-46	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	3e-55	PFAM
coiled coil region	1519	1559	N/A	INTRINSIC
low complexity region	1562	1573	N/A	INTRINSIC
Pfam:Ion_trans	1630	1835	3e-49	PFAM
Pfam:PKD_channel	1688	1842	7.8e-11	PFAM
low complexity region	2180	2211	N/A	INTRINSIC
low complexity region	2230	2246	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100561
AA Change: I809T

PolyPhen 2 Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000098127
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6.1e-55	PFAM
coiled coil region	1542	1582	N/A	INTRINSIC
low complexity region	1585	1596	N/A	INTRINSIC
Pfam:Ion_trans	1653	1858	6.2e-49	PFAM
Pfam:PKD_channel	1711	1865	1.4e-10	PFAM
low complexity region	2203	2234	N/A	INTRINSIC
low complexity region	2253	2269	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000103166
AA Change: I809T

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000099455
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6.1e-55	PFAM
SCOP:d1g2qa_	1545	1589	6e-3	SMART
Pfam:Ion_trans	1646	1851	6.2e-49	PFAM
Pfam:PKD_channel	1704	1858	1.4e-10	PFAM
low complexity region	2196	2227	N/A	INTRINSIC
low complexity region	2246	2262	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107785
AA Change: I809T

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000103414
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.5e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	5.7e-55	PFAM
coiled coil region	1519	1558	N/A	INTRINSIC
Pfam:Ion_trans	1612	1817	5.8e-49	PFAM
Pfam:PKD_channel	1670	1824	1.4e-10	PFAM
low complexity region	2027	2042	N/A	INTRINSIC
low complexity region	2084	2115	N/A	INTRINSIC
low complexity region	2134	2150	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107786
AA Change: I792T

PolyPhen 2 Score 0.527 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000103415
Gene: ENSMUSG00000020866
AA Change: I792T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	383	4e-60	PFAM
low complexity region	466	490	N/A	INTRINSIC
low complexity region	518	530	N/A	INTRINSIC
Pfam:Ion_trans	760	946	1.6e-45	PFAM
low complexity region	1023	1034	N/A	INTRINSIC
low complexity region	1046	1073	N/A	INTRINSIC
low complexity region	1094	1110	N/A	INTRINSIC
low complexity region	1176	1187	N/A	INTRINSIC
Pfam:Ion_trans	1273	1497	5.9e-55	PFAM
SCOP:d1g2qa_	1505	1549	6e-3	SMART
Pfam:Ion_trans	1606	1811	6e-49	PFAM
Pfam:PKD_channel	1664	1818	1.4e-10	PFAM
low complexity region	2156	2187	N/A	INTRINSIC
low complexity region	2206	2222	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107788
AA Change: I809T

PolyPhen 2 Score 0.287 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000103417
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6e-55	PFAM
coiled coil region	1542	1574	N/A	INTRINSIC
Pfam:Ion_trans	1628	1833	6.1e-49	PFAM
Pfam:PKD_channel	1686	1840	1.4e-10	PFAM
low complexity region	2178	2209	N/A	INTRINSIC
low complexity region	2228	2244	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107789
AA Change: I809T

PolyPhen 2 Score 0.287 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000103418
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	80	406	1.6e-76	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	742	972	3.4e-56	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1276	1549	1.5e-61	PFAM
low complexity region	1578	1589	N/A	INTRINSIC
Pfam:Ion_trans	1611	1863	2.1e-56	PFAM
Pfam:PKD_channel	1703	1858	3.4e-9	PFAM
low complexity region	2289	2320	N/A	INTRINSIC
low complexity region	2339	2355	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107790
AA Change: I809T

PolyPhen 2 Score 0.931 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000103419
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	6e-55	PFAM
SCOP:d1g2qa_	1522	1566	6e-3	SMART
Pfam:Ion_trans	1623	1828	6.1e-49	PFAM
Pfam:PKD_channel	1681	1835	1.4e-10	PFAM
low complexity region	2173	2204	N/A	INTRINSIC
low complexity region	2223	2239	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107793
AA Change: I809T

PolyPhen 2 Score 0.551 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000103422
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6e-55	PFAM
coiled coil region	1542	1581	N/A	INTRINSIC
Pfam:Ion_trans	1635	1840	6.1e-49	PFAM
Pfam:PKD_channel	1693	1847	1.4e-10	PFAM
low complexity region	2185	2216	N/A	INTRINSIC
low complexity region	2235	2251	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107791
AA Change: I809T

PolyPhen 2 Score 0.503 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000103420
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	6e-55	PFAM
coiled coil region	1519	1558	N/A	INTRINSIC
Pfam:Ion_trans	1612	1817	6e-49	PFAM
Pfam:PKD_channel	1670	1824	1.4e-10	PFAM
low complexity region	2162	2193	N/A	INTRINSIC
low complexity region	2212	2228	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107792
AA Change: I809T

PolyPhen 2 Score 0.866 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000103421
Gene: ENSMUSG00000020866
AA Change: I809T

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	5.9e-55	PFAM
coiled coil region	1519	1551	N/A	INTRINSIC
Pfam:Ion_trans	1605	1810	6e-49	PFAM
Pfam:PKD_channel	1663	1817	1.4e-10	PFAM
low complexity region	2155	2186	N/A	INTRINSIC
low complexity region	2205	2221	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152811

Meta Mutation Damage Score

0.9497

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.9%

Validation Efficiency

94% (63/67)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. This gene encodes a T-type, low-voltage activated calcium channel. The T-type channels generate currents that are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing. Many alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Homozygote null mice display disrupted sleeping patterns, altered amounts of activity, abnormal action potentials in the brain, prolonged electrical conductance in the heart, and resistance to diet-induced obesity. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	G	A	5: 114,368,781 (GRCm39)	M1666I	possibly damaging	Het
Adamts13	A	T	2: 26,874,153 (GRCm39)	Q434L	probably damaging	Het
Adgrg3	G	A	8: 95,762,562 (GRCm39)	R204H	probably benign	Het
Arhgef28	A	C	13: 98,073,324 (GRCm39)		probably benign	Het
AU041133	T	A	10: 81,987,101 (GRCm39)	C251*	probably null	Het
Axin2	T	A	11: 108,833,794 (GRCm39)	N580K	probably benign	Het
C4b	A	G	17: 34,948,631 (GRCm39)	C1560R	probably damaging	Het
Cacna1s	C	A	1: 136,016,755 (GRCm39)		probably null	Het
Cadps2	T	C	6: 23,688,857 (GRCm39)	S151G	probably damaging	Het
Capn1	T	C	19: 6,040,086 (GRCm39)		probably null	Het
Capn9	A	G	8: 125,302,965 (GRCm39)	S28G	probably benign	Het
Ccdc73	A	G	2: 104,822,637 (GRCm39)	D862G	probably damaging	Het
Cdk17	T	C	10: 93,061,979 (GRCm39)	L237P	probably damaging	Het
Cfap251	A	G	5: 123,440,802 (GRCm39)	I1120V	possibly damaging	Het
Chad	A	C	11: 94,456,384 (GRCm39)	N154T	possibly damaging	Het
Cog1	C	T	11: 113,547,038 (GRCm39)	T544I	probably benign	Het
Cracd	A	T	5: 77,006,470 (GRCm39)	T944S	unknown	Het
Ctnna2	T	C	6: 76,931,830 (GRCm39)	E590G	possibly damaging	Het
Dapk2	T	A	9: 66,072,642 (GRCm39)	M6K	probably benign	Het
Dchs1	T	C	7: 105,421,487 (GRCm39)	E311G	possibly damaging	Het
Ddr2	T	A	1: 169,809,641 (GRCm39)	T779S	probably benign	Het
Dhtkd1	A	G	2: 5,916,744 (GRCm39)	V644A	probably damaging	Het
Dock2	A	G	11: 34,414,934 (GRCm39)	V147A	possibly damaging	Het
Ear10	A	T	14: 44,160,357 (GRCm39)	*157K	probably null	Het
Gm10801	T	C	2: 98,494,197 (GRCm39)	I113T	probably damaging	Het
Gmps	T	G	3: 63,906,049 (GRCm39)	C449G	probably damaging	Het
Grin3a	A	G	4: 49,844,988 (GRCm39)	S32P	possibly damaging	Het
Igkv1-115	A	T	6: 68,138,592 (GRCm39)	D65V	probably damaging	Het
Klk1b4	A	G	7: 43,859,105 (GRCm39)	N41S	probably benign	Het
Krt26	CTAGTA	CTA	11: 99,224,352 (GRCm39)		probably benign	Het
Lrrc8a	T	A	2: 30,145,262 (GRCm39)	D25E	probably damaging	Het
Muc5b	A	G	7: 141,421,960 (GRCm39)	R4426G	possibly damaging	Het
Muc6	G	A	7: 141,218,313 (GRCm39)	S2120F	possibly damaging	Het
Myo5a	G	T	9: 75,023,489 (GRCm39)	D17Y	probably damaging	Het
Nat1	C	G	8: 67,944,076 (GRCm39)	L154V	probably benign	Het
Nek3	T	A	8: 22,647,047 (GRCm39)	T163S	probably damaging	Het
Or2w25	G	T	11: 59,503,949 (GRCm39)	R53L	possibly damaging	Het
Or5p80	A	G	7: 108,229,562 (GRCm39)	D121G	probably damaging	Het
Or6c204	A	G	10: 129,023,044 (GRCm39)	I82T	possibly damaging	Het
Or8d2b	T	A	9: 38,789,147 (GRCm39)	I225N	probably damaging	Het
Or8g32	A	T	9: 39,305,163 (GRCm39)	E22D	possibly damaging	Het
Or9s13	T	C	1: 92,548,525 (GRCm39)	L299P	probably damaging	Het
Osr1	T	G	12: 9,629,268 (GRCm39)	L47R	probably damaging	Het
Pdlim2	C	T	14: 70,402,228 (GRCm39)	R296H	probably damaging	Het
Pik3ap1	T	A	19: 41,291,053 (GRCm39)	N493I	possibly damaging	Het
Polr3e	T	A	7: 120,539,820 (GRCm39)	N522K	probably damaging	Het
Rabgap1	T	A	2: 37,385,771 (GRCm39)		probably null	Het
Rp1l1	C	A	14: 64,268,992 (GRCm39)	A1526E	probably benign	Het
Scn3a	T	A	2: 65,291,878 (GRCm39)	I1623F	probably damaging	Het
Serpinb9e	A	G	13: 33,437,428 (GRCm39)	T104A	probably benign	Het
Sh3rf3	T	C	10: 58,939,989 (GRCm39)		probably benign	Het
Slc30a3	A	G	5: 31,245,748 (GRCm39)	Y213H	probably damaging	Het
Sptbn4	C	T	7: 27,106,563 (GRCm39)	R955H	probably damaging	Het
Tfb1m	A	T	17: 3,569,946 (GRCm39)	Y307N	probably damaging	Het
Tnrc6b	G	T	15: 80,768,407 (GRCm39)		probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Ulk1	A	G	5: 110,938,936 (GRCm39)	Y501H	probably damaging	Het
Zbtb17	A	G	4: 141,191,914 (GRCm39)	H315R	probably damaging	Het
Zeb2	A	G	2: 44,892,624 (GRCm39)	Y142H	probably damaging	Het
Zfr	T	C	15: 12,160,715 (GRCm39)	S763P	possibly damaging	Het

Other mutations in Cacna1g

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01300:Cacna1g	APN	11	94,324,738 (GRCm39)	missense	probably benign	0.16
IGL01382:Cacna1g	APN	11	94,356,684 (GRCm39)	missense	probably damaging	1.00
IGL01694:Cacna1g	APN	11	94,319,938 (GRCm39)	missense	probably damaging	1.00
IGL01866:Cacna1g	APN	11	94,347,937 (GRCm39)	missense	probably damaging	0.99
IGL02341:Cacna1g	APN	11	94,352,978 (GRCm39)	missense	probably damaging	1.00
IGL02506:Cacna1g	APN	11	94,319,955 (GRCm39)	missense	probably damaging	0.99
IGL02706:Cacna1g	APN	11	94,347,818 (GRCm39)	missense	probably damaging	1.00
IGL02879:Cacna1g	APN	11	94,300,431 (GRCm39)	missense	probably benign	0.12
IGL03366:Cacna1g	APN	11	94,347,977 (GRCm39)	missense	probably damaging	1.00
ANU23:Cacna1g	UTSW	11	94,357,054 (GRCm39)	critical splice acceptor site	probably benign
IGL03134:Cacna1g	UTSW	11	94,350,651 (GRCm39)	missense	probably damaging	1.00
R0092:Cacna1g	UTSW	11	94,348,090 (GRCm39)	missense	probably damaging	1.00
R0123:Cacna1g	UTSW	11	94,300,302 (GRCm39)	missense	probably damaging	1.00
R0178:Cacna1g	UTSW	11	94,354,309 (GRCm39)	missense	probably damaging	1.00
R0375:Cacna1g	UTSW	11	94,301,880 (GRCm39)	missense	possibly damaging	0.79
R0389:Cacna1g	UTSW	11	94,350,523 (GRCm39)	missense	probably damaging	1.00
R0433:Cacna1g	UTSW	11	94,350,033 (GRCm39)	missense	probably benign	0.16
R0458:Cacna1g	UTSW	11	94,300,266 (GRCm39)	missense	probably damaging	0.96
R0498:Cacna1g	UTSW	11	94,350,685 (GRCm39)	missense	probably damaging	1.00
R0629:Cacna1g	UTSW	11	94,300,369 (GRCm39)	missense	possibly damaging	0.93
R0800:Cacna1g	UTSW	11	94,317,265 (GRCm39)	missense	probably damaging	1.00
R1341:Cacna1g	UTSW	11	94,324,582 (GRCm39)	missense	probably damaging	0.99
R1457:Cacna1g	UTSW	11	94,350,381 (GRCm39)	missense	possibly damaging	0.76
R1523:Cacna1g	UTSW	11	94,333,555 (GRCm39)	critical splice donor site	probably null
R1532:Cacna1g	UTSW	11	94,334,157 (GRCm39)	missense	probably damaging	1.00
R1540:Cacna1g	UTSW	11	94,347,865 (GRCm39)	missense	probably damaging	1.00
R1652:Cacna1g	UTSW	11	94,318,230 (GRCm39)	missense	probably damaging	1.00
R1688:Cacna1g	UTSW	11	94,316,779 (GRCm39)	missense	possibly damaging	0.70
R1750:Cacna1g	UTSW	11	94,334,118 (GRCm39)	missense	probably damaging	1.00
R1751:Cacna1g	UTSW	11	94,350,628 (GRCm39)	missense	probably benign	0.35
R1767:Cacna1g	UTSW	11	94,350,628 (GRCm39)	missense	probably benign	0.35
R1847:Cacna1g	UTSW	11	94,357,007 (GRCm39)	missense	probably damaging	1.00
R1973:Cacna1g	UTSW	11	94,350,603 (GRCm39)	missense	possibly damaging	0.86
R2050:Cacna1g	UTSW	11	94,300,300 (GRCm39)	missense	probably damaging	1.00
R2261:Cacna1g	UTSW	11	94,347,961 (GRCm39)	missense	probably benign	0.42
R2273:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2274:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2275:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2376:Cacna1g	UTSW	11	94,356,734 (GRCm39)	missense	probably damaging	0.99
R3686:Cacna1g	UTSW	11	94,349,916 (GRCm39)	splice site	probably null
R3809:Cacna1g	UTSW	11	94,306,922 (GRCm39)	missense	probably damaging	1.00
R3875:Cacna1g	UTSW	11	94,328,749 (GRCm39)	missense	probably damaging	0.98
R4133:Cacna1g	UTSW	11	94,323,370 (GRCm39)	missense	probably damaging	1.00
R4642:Cacna1g	UTSW	11	94,308,920 (GRCm39)	missense	probably damaging	1.00
R4732:Cacna1g	UTSW	11	94,334,041 (GRCm39)	missense	probably damaging	1.00
R4733:Cacna1g	UTSW	11	94,334,041 (GRCm39)	missense	probably damaging	1.00
R4773:Cacna1g	UTSW	11	94,302,298 (GRCm39)	missense	possibly damaging	0.78
R4798:Cacna1g	UTSW	11	94,324,673 (GRCm39)	missense	probably damaging	1.00
R4839:Cacna1g	UTSW	11	94,350,433 (GRCm39)	missense	probably benign	0.16
R4900:Cacna1g	UTSW	11	94,350,177 (GRCm39)	missense	possibly damaging	0.78
R4927:Cacna1g	UTSW	11	94,319,973 (GRCm39)	missense	probably damaging	1.00
R4930:Cacna1g	UTSW	11	94,334,899 (GRCm39)	missense	probably damaging	1.00
R5050:Cacna1g	UTSW	11	94,350,541 (GRCm39)	missense	probably damaging	1.00
R5117:Cacna1g	UTSW	11	94,323,329 (GRCm39)	missense	probably damaging	1.00
R5186:Cacna1g	UTSW	11	94,333,674 (GRCm39)	missense	probably damaging	1.00
R5364:Cacna1g	UTSW	11	94,307,684 (GRCm39)	missense	probably benign	0.29
R5512:Cacna1g	UTSW	11	94,334,968 (GRCm39)	missense	probably damaging	1.00
R5564:Cacna1g	UTSW	11	94,321,312 (GRCm39)	missense	probably damaging	1.00
R5603:Cacna1g	UTSW	11	94,330,578 (GRCm39)	missense	possibly damaging	0.76
R5682:Cacna1g	UTSW	11	94,349,940 (GRCm39)	missense	probably damaging	1.00
R5818:Cacna1g	UTSW	11	94,308,946 (GRCm39)	missense	probably damaging	1.00
R5828:Cacna1g	UTSW	11	94,347,980 (GRCm39)	missense	probably damaging	1.00
R5882:Cacna1g	UTSW	11	94,350,645 (GRCm39)	missense	probably damaging	1.00
R5884:Cacna1g	UTSW	11	94,328,693 (GRCm39)	missense	probably damaging	1.00
R6075:Cacna1g	UTSW	11	94,307,491 (GRCm39)	missense	probably damaging	1.00
R6112:Cacna1g	UTSW	11	94,300,072 (GRCm39)	missense	probably damaging	0.98
R6122:Cacna1g	UTSW	11	94,320,997 (GRCm39)	missense	probably benign	0.11
R6145:Cacna1g	UTSW	11	94,353,087 (GRCm39)	missense	probably damaging	1.00
R6362:Cacna1g	UTSW	11	94,330,533 (GRCm39)	critical splice donor site	probably null
R6415:Cacna1g	UTSW	11	94,354,243 (GRCm39)	missense	probably damaging	1.00
R6468:Cacna1g	UTSW	11	94,330,548 (GRCm39)	missense	probably damaging	1.00
R6648:Cacna1g	UTSW	11	94,323,395 (GRCm39)	missense	probably damaging	1.00
R6746:Cacna1g	UTSW	11	94,300,253 (GRCm39)	nonsense	probably null
R6764:Cacna1g	UTSW	11	94,304,014 (GRCm39)	missense	possibly damaging	0.95
R6782:Cacna1g	UTSW	11	94,350,376 (GRCm39)	missense	probably damaging	1.00
R6888:Cacna1g	UTSW	11	94,350,033 (GRCm39)	missense	probably benign	0.16
R7148:Cacna1g	UTSW	11	94,356,756 (GRCm39)	missense	probably benign	0.32
R7181:Cacna1g	UTSW	11	94,306,691 (GRCm39)	missense	probably benign	0.21
R7183:Cacna1g	UTSW	11	94,330,563 (GRCm39)	missense	probably benign	0.04
R7193:Cacna1g	UTSW	11	94,300,057 (GRCm39)	missense	possibly damaging	0.60
R7237:Cacna1g	UTSW	11	94,328,705 (GRCm39)	missense	probably benign	0.21
R7254:Cacna1g	UTSW	11	94,323,393 (GRCm39)	nonsense	probably null
R7312:Cacna1g	UTSW	11	94,323,383 (GRCm39)	missense	probably damaging	1.00
R7451:Cacna1g	UTSW	11	94,319,901 (GRCm39)	missense	probably damaging	1.00
R7470:Cacna1g	UTSW	11	94,352,765 (GRCm39)	missense	possibly damaging	0.76
R7534:Cacna1g	UTSW	11	94,301,904 (GRCm39)	missense	probably benign	0.00
R7585:Cacna1g	UTSW	11	94,364,368 (GRCm39)	missense	probably benign	0.39
R7706:Cacna1g	UTSW	11	94,305,867 (GRCm39)	missense	probably benign	0.06
R7812:Cacna1g	UTSW	11	94,334,880 (GRCm39)	missense	probably benign	0.03
R7918:Cacna1g	UTSW	11	94,334,856 (GRCm39)	missense	probably benign	0.03
R7947:Cacna1g	UTSW	11	94,348,001 (GRCm39)	missense	probably benign	0.01
R8013:Cacna1g	UTSW	11	94,347,796 (GRCm39)	missense	probably damaging	0.99
R8016:Cacna1g	UTSW	11	94,334,007 (GRCm39)	missense	probably benign	0.36
R8029:Cacna1g	UTSW	11	94,300,564 (GRCm39)	missense	probably benign	0.01
R8098:Cacna1g	UTSW	11	94,307,338 (GRCm39)	missense	probably benign	0.20
R8264:Cacna1g	UTSW	11	94,364,392 (GRCm39)	missense	probably benign	0.21
R8478:Cacna1g	UTSW	11	94,317,278 (GRCm39)	missense	probably damaging	1.00
R8679:Cacna1g	UTSW	11	94,319,962 (GRCm39)	missense	probably damaging	1.00
R8697:Cacna1g	UTSW	11	94,307,524 (GRCm39)	missense	probably benign	0.09
R8772:Cacna1g	UTSW	11	94,356,713 (GRCm39)	missense	probably benign	0.03
R9011:Cacna1g	UTSW	11	94,306,663 (GRCm39)	missense	probably benign	0.21
R9085:Cacna1g	UTSW	11	94,334,046 (GRCm39)	missense	probably benign	0.01
R9155:Cacna1g	UTSW	11	94,350,423 (GRCm39)	missense
R9243:Cacna1g	UTSW	11	94,347,893 (GRCm39)	missense	possibly damaging	0.84
R9288:Cacna1g	UTSW	11	94,308,897 (GRCm39)	nonsense	probably null
R9408:Cacna1g	UTSW	11	94,321,050 (GRCm39)	missense	probably damaging	1.00
R9496:Cacna1g	UTSW	11	94,356,711 (GRCm39)	missense	probably benign	0.03
R9607:Cacna1g	UTSW	11	94,356,714 (GRCm39)	missense	probably benign	0.03
R9720:Cacna1g	UTSW	11	94,302,297 (GRCm39)	missense	probably benign	0.01
X0001:Cacna1g	UTSW	11	94,300,471 (GRCm39)	missense	possibly damaging	0.90
X0019:Cacna1g	UTSW	11	94,350,079 (GRCm39)	missense	probably damaging	0.97
X0065:Cacna1g	UTSW	11	94,353,251 (GRCm39)	missense	probably damaging	1.00
Z1176:Cacna1g	UTSW	11	94,328,937 (GRCm39)	missense	probably benign	0.31
Z1177:Cacna1g	UTSW	11	94,364,416 (GRCm39)	missense	probably benign	0.21
Z1177:Cacna1g	UTSW	11	94,350,422 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TTGAGAGCCAGCTTTCCAAC -3'
(R):5'- CCCATGTTCAGGATGTCTGG -3'

Sequencing Primer
(F):5'- TCATCCACCCCAGGCCTG -3'
(R):5'- CATGTTCAGGATGTCTGGGATGTG -3'

Posted On

2014-07-14