Mutagenetix > Incidental Mutation

Incidental Mutation 'R1907:Pclo'

214446

Institutional Source

Beutler Lab

Gene Symbol

Pclo

Ensembl Gene

ENSMUSG00000061601

Gene Name

piccolo (presynaptic cytomatrix protein)

Synonyms

Acz, Pico

MMRRC Submission

039926-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1907 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

14564932-14913473 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to G at 14728525 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030691] [ENSMUST00000182407]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000030691
AA Change: D2461G

SMART Domains

Protein: ENSMUSP00000030691
Gene: ENSMUSG00000061601
AA Change: D2461G

Domain	Start	End	E-Value	Type
low complexity region	4	30	N/A	INTRINSIC
low complexity region	90	106	N/A	INTRINSIC
internal_repeat_2	192	304	1.23e-13	PROSPERO
internal_repeat_2	305	389	1.23e-13	PROSPERO
low complexity region	393	489	N/A	INTRINSIC
low complexity region	513	525	N/A	INTRINSIC
Pfam:zf-piccolo	530	589	5.2e-30	PFAM
low complexity region	636	647	N/A	INTRINSIC
low complexity region	657	686	N/A	INTRINSIC
low complexity region	709	720	N/A	INTRINSIC
low complexity region	722	738	N/A	INTRINSIC
low complexity region	798	824	N/A	INTRINSIC
low complexity region	825	839	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	961	978	N/A	INTRINSIC
Pfam:zf-piccolo	995	1053	6.7e-33	PFAM
low complexity region	1070	1090	N/A	INTRINSIC
low complexity region	1092	1107	N/A	INTRINSIC
low complexity region	1150	1179	N/A	INTRINSIC
internal_repeat_4	1196	1249	1.65e-5	PROSPERO
low complexity region	1275	1289	N/A	INTRINSIC
low complexity region	1320	1331	N/A	INTRINSIC
low complexity region	1332	1343	N/A	INTRINSIC
low complexity region	1397	1411	N/A	INTRINSIC
low complexity region	1444	1457	N/A	INTRINSIC
low complexity region	1497	1511	N/A	INTRINSIC
low complexity region	1668	1681	N/A	INTRINSIC
coiled coil region	1704	1748	N/A	INTRINSIC
low complexity region	1789	1800	N/A	INTRINSIC
low complexity region	2046	2061	N/A	INTRINSIC
low complexity region	2109	2137	N/A	INTRINSIC
low complexity region	2335	2366	N/A	INTRINSIC
low complexity region	2374	2389	N/A	INTRINSIC
low complexity region	2413	2423	N/A	INTRINSIC
low complexity region	2487	2498	N/A	INTRINSIC
low complexity region	2605	2633	N/A	INTRINSIC
low complexity region	2727	2738	N/A	INTRINSIC
low complexity region	2792	2808	N/A	INTRINSIC
low complexity region	3117	3128	N/A	INTRINSIC
coiled coil region	3136	3189	N/A	INTRINSIC
coiled coil region	3210	3243	N/A	INTRINSIC
internal_repeat_4	3473	3521	1.65e-5	PROSPERO
coiled coil region	3688	3731	N/A	INTRINSIC
low complexity region	3735	3750	N/A	INTRINSIC
low complexity region	3863	3883	N/A	INTRINSIC
low complexity region	3947	3961	N/A	INTRINSIC
low complexity region	4004	4015	N/A	INTRINSIC
low complexity region	4149	4160	N/A	INTRINSIC
low complexity region	4185	4196	N/A	INTRINSIC
low complexity region	4210	4225	N/A	INTRINSIC
low complexity region	4229	4245	N/A	INTRINSIC
PDZ	4439	4518	3.74e-14	SMART
low complexity region	4575	4602	N/A	INTRINSIC
C2	4636	4750	1.44e-21	SMART
low complexity region	4764	4778	N/A	INTRINSIC
low complexity region	4801	4817	N/A	INTRINSIC
low complexity region	4840	4850	N/A	INTRINSIC
low complexity region	4870	4892	N/A	INTRINSIC
C2	4951	5057	8.56e-15	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000182407
AA Change: D2461G

SMART Domains

Protein: ENSMUSP00000138419
Gene: ENSMUSG00000061601
AA Change: D2461G

Domain	Start	End	E-Value	Type
low complexity region	4	30	N/A	INTRINSIC
low complexity region	90	106	N/A	INTRINSIC
internal_repeat_2	192	304	1.06e-13	PROSPERO
internal_repeat_2	305	389	1.06e-13	PROSPERO
low complexity region	393	489	N/A	INTRINSIC
low complexity region	513	525	N/A	INTRINSIC
Pfam:zf-piccolo	528	589	3e-36	PFAM
low complexity region	636	647	N/A	INTRINSIC
low complexity region	657	686	N/A	INTRINSIC
low complexity region	709	720	N/A	INTRINSIC
low complexity region	722	738	N/A	INTRINSIC
low complexity region	798	824	N/A	INTRINSIC
low complexity region	825	839	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	961	978	N/A	INTRINSIC
Pfam:zf-piccolo	993	1053	4.3e-37	PFAM
low complexity region	1070	1090	N/A	INTRINSIC
low complexity region	1092	1107	N/A	INTRINSIC
low complexity region	1150	1179	N/A	INTRINSIC
internal_repeat_3	1196	1249	1.46e-5	PROSPERO
low complexity region	1275	1289	N/A	INTRINSIC
low complexity region	1320	1331	N/A	INTRINSIC
low complexity region	1332	1343	N/A	INTRINSIC
low complexity region	1397	1411	N/A	INTRINSIC
low complexity region	1444	1457	N/A	INTRINSIC
low complexity region	1497	1511	N/A	INTRINSIC
low complexity region	1668	1681	N/A	INTRINSIC
coiled coil region	1704	1748	N/A	INTRINSIC
low complexity region	1789	1800	N/A	INTRINSIC
low complexity region	2046	2061	N/A	INTRINSIC
low complexity region	2109	2137	N/A	INTRINSIC
low complexity region	2335	2366	N/A	INTRINSIC
low complexity region	2374	2389	N/A	INTRINSIC
low complexity region	2413	2423	N/A	INTRINSIC
low complexity region	2487	2498	N/A	INTRINSIC
low complexity region	2605	2633	N/A	INTRINSIC
low complexity region	2727	2738	N/A	INTRINSIC
low complexity region	2792	2808	N/A	INTRINSIC
low complexity region	3117	3128	N/A	INTRINSIC
coiled coil region	3136	3189	N/A	INTRINSIC
coiled coil region	3210	3243	N/A	INTRINSIC
internal_repeat_3	3473	3521	1.46e-5	PROSPERO
coiled coil region	3688	3731	N/A	INTRINSIC
low complexity region	3735	3750	N/A	INTRINSIC
low complexity region	3863	3883	N/A	INTRINSIC
low complexity region	3947	3961	N/A	INTRINSIC
low complexity region	4004	4015	N/A	INTRINSIC
low complexity region	4149	4160	N/A	INTRINSIC
low complexity region	4185	4196	N/A	INTRINSIC
low complexity region	4210	4225	N/A	INTRINSIC
low complexity region	4229	4245	N/A	INTRINSIC
PDZ	4439	4518	3.74e-14	SMART
low complexity region	4575	4602	N/A	INTRINSIC
C2	4636	4750	1.44e-21	SMART
low complexity region	4764	4778	N/A	INTRINSIC
low complexity region	4801	4817	N/A	INTRINSIC
low complexity region	4840	4850	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182426

Predicted Effect

probably benign
Transcript: ENSMUST00000182915

Meta Mutation Damage Score

0.0915

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.6%
20x: 93.4%

Validation Efficiency

98% (104/106)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of the presynaptic cytoskeletal matrix, which is involved in establishing active synaptic zones and in synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
PHENOTYPE: Mice homozygous for one deletion of Pclo are viable and fertile, and display no overt abnormal phenotype. Mice homozygous for another knock-out allele exhibit some premature lethality, decreased body size, and abnormal synaptic vesicle number. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted(5) Gene trapped(2)

Other mutations in this stock

Total: 104 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	C	A	3: 121,862,661 (GRCm39)	S206R	probably damaging	Het
Abcc6	G	T	7: 45,663,593 (GRCm39)	A357E	probably benign	Het
Abhd13	T	A	8: 10,038,170 (GRCm39)	C256S	probably benign	Het
Actrt3	A	G	3: 30,652,716 (GRCm39)	V126A	probably damaging	Het
Adgrv1	A	G	13: 81,740,670 (GRCm39)		probably benign	Het
Alyref2	A	G	1: 171,331,838 (GRCm39)	D205G	probably damaging	Het
Amigo3	G	A	9: 107,930,835 (GRCm39)	R86Q	probably benign	Het
Ankrd50	G	C	3: 38,508,201 (GRCm39)	P1389A	probably damaging	Het
Aoah	A	G	13: 21,094,264 (GRCm39)	D183G	probably damaging	Het
Arap3	A	T	18: 38,129,724 (GRCm39)	S146T	probably benign	Het
Atp2c2	T	C	8: 120,476,615 (GRCm39)		probably benign	Het
Atp8b2	C	T	3: 89,853,583 (GRCm39)	V682M	probably benign	Het
Cacna1i	T	C	15: 80,259,465 (GRCm39)	I1245T	probably damaging	Het
Card14	A	G	11: 119,222,085 (GRCm39)	N460S	probably benign	Het
Ceacam5	A	G	7: 17,486,309 (GRCm39)	D602G	possibly damaging	Het
Cecr2	T	A	6: 120,738,121 (GRCm39)	H921Q	probably benign	Het
Cyb5d1	T	C	11: 69,285,566 (GRCm39)	D115G	probably benign	Het
Dbp	C	T	7: 45,357,744 (GRCm39)	T67I	possibly damaging	Het
Dennd5b	C	T	6: 148,943,074 (GRCm39)	V601I	probably benign	Het
Dnah11	G	A	12: 118,091,291 (GRCm39)	A947V	possibly damaging	Het
Dpysl3	T	A	18: 43,571,193 (GRCm39)	D27V	probably damaging	Het
Drd1	A	T	13: 54,207,271 (GRCm39)	D307E	possibly damaging	Het
Dscaml1	G	A	9: 45,651,778 (GRCm39)	G277D	probably damaging	Het
Eif4g3	C	T	4: 137,885,726 (GRCm39)	R842W	probably damaging	Het
Enam	C	T	5: 88,652,481 (GRCm39)	T1330I	possibly damaging	Het
Fabp6	T	C	11: 43,486,994 (GRCm39)		probably null	Het
Fcsk	A	T	8: 111,620,010 (GRCm39)	L289*	probably null	Het
Fhip1a	T	C	3: 85,579,940 (GRCm39)	D755G	probably benign	Het
Fsip2	C	T	2: 82,813,772 (GRCm39)	P3364S	possibly damaging	Het
Gad1	C	T	2: 70,409,482 (GRCm39)	S191F	possibly damaging	Het
Gcm1	A	C	9: 77,972,055 (GRCm39)	N332T	probably benign	Het
Gin1	G	A	1: 97,703,172 (GRCm39)		probably benign	Het
Gpr25	T	C	1: 136,188,538 (GRCm39)	D25G	probably benign	Het
Gstm4	T	C	3: 107,948,593 (GRCm39)	T172A	probably benign	Het
Hnrnpll	A	G	17: 80,342,758 (GRCm39)		probably null	Het
Il12rb2	T	A	6: 67,272,270 (GRCm39)	K339*	probably null	Het
Inpp1	A	G	1: 52,828,829 (GRCm39)	*397Q	probably null	Het
Kcp	G	T	6: 29,497,834 (GRCm39)		probably benign	Het
Kdm1b	G	A	13: 47,217,596 (GRCm39)	V352I	probably benign	Het
Klhdc2	G	T	12: 69,343,734 (GRCm39)		probably benign	Het
Krt75	T	A	15: 101,481,801 (GRCm39)	T156S	possibly damaging	Het
Lag3	T	A	6: 124,886,450 (GRCm39)	I168F	possibly damaging	Het
Lama4	T	G	10: 38,948,754 (GRCm39)	V839G	probably benign	Het
Lmtk2	G	T	5: 144,111,928 (GRCm39)	V883L	probably benign	Het
Lrrc37a	C	A	11: 103,347,982 (GRCm39)	K2904N	unknown	Het
Macf1	A	T	4: 123,266,192 (GRCm39)	I4775N	probably damaging	Het
Map2k3	T	G	11: 60,823,055 (GRCm39)	S3A	possibly damaging	Het
Mast1	G	A	8: 85,642,895 (GRCm39)	R967C	probably damaging	Het
Mettl2	T	G	11: 105,017,666 (GRCm39)	S59A	probably benign	Het
Mtr	G	A	13: 12,240,418 (GRCm39)	T530I	probably damaging	Het
Myo7b	A	T	18: 32,110,052 (GRCm39)	C1137S	possibly damaging	Het
Nacad	T	A	11: 6,552,540 (GRCm39)	H217L	probably benign	Het
Ncstn	A	T	1: 171,899,710 (GRCm39)	V324E	probably damaging	Het
Ndufaf7	C	A	17: 79,249,546 (GRCm39)	H148N	possibly damaging	Het
Or10a49	T	A	7: 108,467,705 (GRCm39)	I219F	possibly damaging	Het
Or52a24	T	A	7: 103,381,190 (GRCm39)	I19N	probably damaging	Het
Or52n2b	G	A	7: 104,566,272 (GRCm39)	T77I	probably damaging	Het
Or7g12	T	C	9: 18,899,737 (GRCm39)	I151T	possibly damaging	Het
Or8b12	T	G	9: 37,657,729 (GRCm39)	L100V	probably benign	Het
Or8k17	T	C	2: 86,066,454 (GRCm39)	K242E	possibly damaging	Het
Pcdh20	T	C	14: 88,706,140 (GRCm39)	I387V	probably benign	Het
Pcp2	G	A	8: 3,674,904 (GRCm39)		probably benign	Het
Pde1a	T	A	2: 79,698,651 (GRCm39)	D393V	probably damaging	Het
Pias4	T	C	10: 80,990,197 (GRCm39)	D421G	possibly damaging	Het
Pign	T	C	1: 105,565,940 (GRCm39)	T362A	possibly damaging	Het
Pik3c2g	A	C	6: 139,789,768 (GRCm39)	K304Q	probably damaging	Het
Pkd2	T	A	5: 104,634,672 (GRCm39)	W568R	probably damaging	Het
Pld2	T	A	11: 70,435,010 (GRCm39)	I306N	probably damaging	Het
Plppr3	T	C	10: 79,709,903 (GRCm39)	K9R	probably damaging	Het
Pramel11	T	A	4: 143,622,061 (GRCm39)	R431S	possibly damaging	Het
Psme4	T	C	11: 30,760,922 (GRCm39)	V397A	probably damaging	Het
Ptbp2	A	T	3: 119,555,398 (GRCm39)	S23R	probably damaging	Het
Ptpn13	A	G	5: 103,728,575 (GRCm39)	D2074G	probably null	Het
Rab34	T	A	11: 78,082,081 (GRCm39)	L166H	probably damaging	Het
Rabgap1l	T	A	1: 160,472,880 (GRCm39)	R519S	probably benign	Het
Rasa1	A	T	13: 85,374,691 (GRCm39)	L218*	probably null	Het
Reln	A	G	5: 22,249,960 (GRCm39)		probably null	Het
Rer1	T	C	4: 155,162,956 (GRCm39)	D94G	possibly damaging	Het
Rhpn1	T	C	15: 75,583,673 (GRCm39)	V386A	probably benign	Het
Samd3	T	A	10: 26,147,754 (GRCm39)	C476*	probably null	Het
Sdk2	C	T	11: 113,729,472 (GRCm39)		silent	Het
Sipa1l3	A	G	7: 29,038,592 (GRCm39)	S352P	possibly damaging	Het
Slc27a2	A	G	2: 126,428,262 (GRCm39)	Y549C	probably benign	Het
Slc7a9	A	G	7: 35,149,279 (GRCm39)	T7A	probably benign	Het
Slco4c1	C	T	1: 96,770,224 (GRCm39)	G280D	probably damaging	Het
Snx19	T	A	9: 30,344,872 (GRCm39)	V657E	probably damaging	Het
Spata31d1c	A	G	13: 65,183,690 (GRCm39)	M411V	probably benign	Het
Spice1	T	A	16: 44,178,193 (GRCm39)	L72*	probably null	Het
Stard9	G	T	2: 120,544,293 (GRCm39)	V4471L	probably damaging	Het
Syt14	A	G	1: 192,584,143 (GRCm39)	L707P	probably damaging	Het
Tab1	T	C	15: 80,037,869 (GRCm39)	I234T	probably damaging	Het
Tas2r107	T	C	6: 131,636,951 (GRCm39)	M33V	probably benign	Het
Tbx4	T	A	11: 85,805,349 (GRCm39)	S479R	possibly damaging	Het
Tssk5	C	T	15: 76,257,093 (GRCm39)	R263Q	probably benign	Het
Ttn	T	C	2: 76,701,200 (GRCm39)		probably benign	Het
Ugt2b5	T	A	5: 87,287,489 (GRCm39)	Q226L	probably benign	Het
Ush2a	C	T	1: 188,447,261 (GRCm39)	S2483L	probably benign	Het
Wdr95	A	G	5: 149,475,891 (GRCm39)	Y63C	probably damaging	Het
Xirp2	A	G	2: 67,346,700 (GRCm39)	I2980M	probably damaging	Het
Yeats4	G	A	10: 117,051,636 (GRCm39)	T207I	probably benign	Het
Ythdc1	A	G	5: 86,978,489 (GRCm39)	R561G	probably damaging	Het
Zbtb14	G	A	17: 69,694,385 (GRCm39)	E28K	possibly damaging	Het
Zfp931	A	T	2: 177,711,684 (GRCm39)	L21Q	probably damaging	Het
Zyg11b	A	C	4: 108,112,423 (GRCm39)	M415R	probably damaging	Het

Other mutations in Pclo

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00333:Pclo	APN	5	14,571,691 (GRCm39)	nonsense	probably null
IGL00429:Pclo	APN	5	14,730,753 (GRCm39)	unclassified	probably benign
IGL00498:Pclo	APN	5	14,590,753 (GRCm39)	missense	unknown
IGL00528:Pclo	APN	5	14,726,448 (GRCm39)	unclassified	probably benign
IGL00743:Pclo	APN	5	14,728,035 (GRCm39)	unclassified	probably benign
IGL00809:Pclo	APN	5	14,725,811 (GRCm39)	missense	unknown
IGL00811:Pclo	APN	5	14,730,024 (GRCm39)	unclassified	probably benign
IGL00819:Pclo	APN	5	14,908,860 (GRCm39)	missense	unknown
IGL00870:Pclo	APN	5	14,589,997 (GRCm39)	missense	unknown
IGL00925:Pclo	APN	5	14,816,755 (GRCm39)	missense	unknown
IGL00960:Pclo	APN	5	14,725,234 (GRCm39)	missense	unknown
IGL01013:Pclo	APN	5	14,843,848 (GRCm39)	missense	unknown
IGL01112:Pclo	APN	5	14,731,083 (GRCm39)	missense	unknown
IGL01124:Pclo	APN	5	14,764,343 (GRCm39)	missense	unknown
IGL01150:Pclo	APN	5	14,726,926 (GRCm39)	unclassified	probably benign
IGL01302:Pclo	APN	5	14,726,013 (GRCm39)	unclassified	probably benign
IGL01389:Pclo	APN	5	14,764,535 (GRCm39)	missense	probably damaging	0.99
IGL01448:Pclo	APN	5	14,726,408 (GRCm39)	unclassified	probably benign
IGL01449:Pclo	APN	5	14,728,530 (GRCm39)	unclassified	probably benign
IGL01450:Pclo	APN	5	14,727,207 (GRCm39)	unclassified	probably benign
IGL01476:Pclo	APN	5	14,571,122 (GRCm39)	missense	probably damaging	1.00
IGL01537:Pclo	APN	5	14,589,647 (GRCm39)	missense	unknown
IGL01568:Pclo	APN	5	14,728,443 (GRCm39)	unclassified	probably benign
IGL01574:Pclo	APN	5	14,763,462 (GRCm39)	missense	unknown
IGL01637:Pclo	APN	5	14,590,048 (GRCm39)	missense	unknown
IGL01646:Pclo	APN	5	14,763,881 (GRCm39)	missense	unknown
IGL01672:Pclo	APN	5	14,728,549 (GRCm39)	unclassified	probably benign
IGL01705:Pclo	APN	5	14,727,879 (GRCm39)	unclassified	probably benign
IGL02127:Pclo	APN	5	14,815,159 (GRCm39)	splice site	probably benign
IGL02215:Pclo	APN	5	14,906,999 (GRCm39)	missense	unknown
IGL02271:Pclo	APN	5	14,729,508 (GRCm39)	unclassified	probably benign
IGL02300:Pclo	APN	5	14,763,755 (GRCm39)	missense	unknown
IGL02346:Pclo	APN	5	14,727,552 (GRCm39)	unclassified	probably benign
IGL02449:Pclo	APN	5	14,565,357 (GRCm39)	missense	probably damaging	1.00
IGL02452:Pclo	APN	5	14,726,980 (GRCm39)	unclassified	probably benign
IGL02455:Pclo	APN	5	14,590,189 (GRCm39)	missense	unknown
IGL02478:Pclo	APN	5	14,816,792 (GRCm39)	missense	unknown
IGL02677:Pclo	APN	5	14,726,943 (GRCm39)	unclassified	probably benign
IGL02711:Pclo	APN	5	14,572,322 (GRCm39)	missense	unknown
IGL02737:Pclo	APN	5	14,764,177 (GRCm39)	missense	unknown
IGL02943:Pclo	APN	5	14,719,235 (GRCm39)	missense	unknown
IGL03184:Pclo	APN	5	14,764,457 (GRCm39)	missense	probably damaging	1.00
IGL03246:Pclo	APN	5	14,727,631 (GRCm39)	unclassified	probably benign
IGL03263:Pclo	APN	5	14,731,824 (GRCm39)	missense	unknown
IGL03300:Pclo	APN	5	14,762,812 (GRCm39)	missense	unknown
IGL03399:Pclo	APN	5	14,816,745 (GRCm39)	missense	unknown
Gauche	UTSW	5	14,843,869 (GRCm39)	missense	unknown
P0018:Pclo	UTSW	5	14,727,735 (GRCm39)	unclassified	probably benign
PIT4472001:Pclo	UTSW	5	14,763,182 (GRCm39)	missense	possibly damaging	0.54
R0014:Pclo	UTSW	5	14,730,465 (GRCm39)	unclassified	probably benign
R0014:Pclo	UTSW	5	14,730,465 (GRCm39)	unclassified	probably benign
R0020:Pclo	UTSW	5	14,719,687 (GRCm39)	missense	unknown
R0045:Pclo	UTSW	5	14,589,485 (GRCm39)	missense	unknown
R0046:Pclo	UTSW	5	14,590,493 (GRCm39)	missense	unknown
R0046:Pclo	UTSW	5	14,590,493 (GRCm39)	missense	unknown
R0128:Pclo	UTSW	5	14,729,811 (GRCm39)	unclassified	probably benign
R0130:Pclo	UTSW	5	14,729,811 (GRCm39)	unclassified	probably benign
R0141:Pclo	UTSW	5	14,841,936 (GRCm39)	missense	unknown
R0226:Pclo	UTSW	5	14,815,237 (GRCm39)	missense	probably damaging	0.99
R0243:Pclo	UTSW	5	14,825,434 (GRCm39)	missense	unknown
R0267:Pclo	UTSW	5	14,731,194 (GRCm39)	missense	unknown
R0313:Pclo	UTSW	5	14,728,887 (GRCm39)	unclassified	probably benign
R0324:Pclo	UTSW	5	14,719,447 (GRCm39)	missense	unknown
R0331:Pclo	UTSW	5	14,730,390 (GRCm39)	unclassified	probably benign
R0370:Pclo	UTSW	5	14,571,104 (GRCm39)	missense	probably damaging	1.00
R0398:Pclo	UTSW	5	14,731,716 (GRCm39)	missense	unknown
R0401:Pclo	UTSW	5	14,731,748 (GRCm39)	missense	unknown
R0417:Pclo	UTSW	5	14,763,036 (GRCm39)	missense	unknown
R0468:Pclo	UTSW	5	14,727,302 (GRCm39)	unclassified	probably benign
R0472:Pclo	UTSW	5	14,731,608 (GRCm39)	missense	unknown
R0488:Pclo	UTSW	5	14,719,313 (GRCm39)	missense	unknown
R0511:Pclo	UTSW	5	14,729,412 (GRCm39)	unclassified	probably benign
R0511:Pclo	UTSW	5	14,728,299 (GRCm39)	unclassified	probably benign
R0520:Pclo	UTSW	5	14,763,844 (GRCm39)	nonsense	probably null
R0547:Pclo	UTSW	5	14,842,086 (GRCm39)	missense	unknown
R0611:Pclo	UTSW	5	14,762,828 (GRCm39)	missense	unknown
R0611:Pclo	UTSW	5	14,728,789 (GRCm39)	unclassified	probably benign
R0624:Pclo	UTSW	5	14,719,670 (GRCm39)	missense	unknown
R0628:Pclo	UTSW	5	14,719,552 (GRCm39)	missense	unknown
R0639:Pclo	UTSW	5	14,731,763 (GRCm39)	nonsense	probably null
R0653:Pclo	UTSW	5	14,732,269 (GRCm39)	intron	probably benign
R0681:Pclo	UTSW	5	14,725,332 (GRCm39)	missense	unknown
R0689:Pclo	UTSW	5	14,764,033 (GRCm39)	missense	unknown
R0698:Pclo	UTSW	5	14,762,530 (GRCm39)	missense	unknown
R0737:Pclo	UTSW	5	14,565,453 (GRCm39)	missense	probably damaging	1.00
R0883:Pclo	UTSW	5	14,727,873 (GRCm39)	nonsense	probably null
R0906:Pclo	UTSW	5	14,726,700 (GRCm39)	unclassified	probably benign
R1056:Pclo	UTSW	5	14,590,069 (GRCm39)	nonsense	probably null
R1107:Pclo	UTSW	5	14,727,883 (GRCm39)	unclassified	probably benign
R1174:Pclo	UTSW	5	14,727,660 (GRCm39)	unclassified	probably benign
R1184:Pclo	UTSW	5	14,572,276 (GRCm39)	missense	unknown
R1302:Pclo	UTSW	5	14,731,647 (GRCm39)	missense	unknown
R1318:Pclo	UTSW	5	14,729,328 (GRCm39)	unclassified	probably benign
R1342:Pclo	UTSW	5	14,732,191 (GRCm39)	intron	probably benign
R1378:Pclo	UTSW	5	14,732,327 (GRCm39)	missense	probably benign	0.23
R1418:Pclo	UTSW	5	14,728,144 (GRCm39)	unclassified	probably benign
R1471:Pclo	UTSW	5	14,730,441 (GRCm39)	unclassified	probably benign
R1485:Pclo	UTSW	5	14,763,793 (GRCm39)	missense	unknown
R1523:Pclo	UTSW	5	14,838,420 (GRCm39)	missense	unknown
R1527:Pclo	UTSW	5	14,729,662 (GRCm39)	unclassified	probably benign
R1531:Pclo	UTSW	5	14,571,917 (GRCm39)	missense	probably damaging	0.99
R1537:Pclo	UTSW	5	14,762,489 (GRCm39)	missense	unknown
R1574:Pclo	UTSW	5	14,729,845 (GRCm39)	unclassified	probably benign
R1574:Pclo	UTSW	5	14,729,845 (GRCm39)	unclassified	probably benign
R1581:Pclo	UTSW	5	14,571,296 (GRCm39)	missense	probably benign	0.28
R1613:Pclo	UTSW	5	14,729,146 (GRCm39)	unclassified	probably benign
R1632:Pclo	UTSW	5	14,730,017 (GRCm39)	unclassified	probably benign
R1688:Pclo	UTSW	5	14,838,507 (GRCm39)	critical splice donor site	probably null
R1694:Pclo	UTSW	5	14,570,977 (GRCm39)	missense	probably damaging	1.00
R1707:Pclo	UTSW	5	14,763,238 (GRCm39)	missense	unknown
R1727:Pclo	UTSW	5	14,727,001 (GRCm39)	unclassified	probably benign
R1741:Pclo	UTSW	5	14,726,524 (GRCm39)	unclassified	probably benign
R1853:Pclo	UTSW	5	14,726,698 (GRCm39)	unclassified	probably benign
R1856:Pclo	UTSW	5	14,828,566 (GRCm39)	missense	probably damaging	1.00
R1970:Pclo	UTSW	5	14,763,487 (GRCm39)	missense	unknown
R1971:Pclo	UTSW	5	14,763,487 (GRCm39)	missense	unknown
R1973:Pclo	UTSW	5	14,726,073 (GRCm39)	splice site	probably null
R1978:Pclo	UTSW	5	14,763,809 (GRCm39)	missense	unknown
R1999:Pclo	UTSW	5	14,727,094 (GRCm39)	unclassified	probably benign
R2015:Pclo	UTSW	5	14,571,515 (GRCm39)	missense	probably damaging	0.98
R2084:Pclo	UTSW	5	14,732,162 (GRCm39)	missense	probably benign	0.23
R2144:Pclo	UTSW	5	14,908,766 (GRCm39)	missense	unknown
R2155:Pclo	UTSW	5	14,764,309 (GRCm39)	missense	probably benign	0.02
R2191:Pclo	UTSW	5	14,763,862 (GRCm39)	missense	unknown
R2237:Pclo	UTSW	5	14,763,952 (GRCm39)	missense	unknown
R2276:Pclo	UTSW	5	14,764,287 (GRCm39)	missense	unknown
R2279:Pclo	UTSW	5	14,764,287 (GRCm39)	missense	unknown
R2281:Pclo	UTSW	5	14,590,346 (GRCm39)	missense	unknown
R2307:Pclo	UTSW	5	14,728,665 (GRCm39)	unclassified	probably benign
R2386:Pclo	UTSW	5	14,815,261 (GRCm39)	missense	unknown
R2407:Pclo	UTSW	5	14,728,946 (GRCm39)	unclassified	probably benign
R2512:Pclo	UTSW	5	14,762,612 (GRCm39)	missense	unknown
R2889:Pclo	UTSW	5	14,906,995 (GRCm39)	missense	unknown
R2966:Pclo	UTSW	5	14,731,164 (GRCm39)	missense	unknown
R3151:Pclo	UTSW	5	14,571,692 (GRCm39)	missense	probably damaging	0.99
R3424:Pclo	UTSW	5	14,730,432 (GRCm39)	unclassified	probably benign
R3687:Pclo	UTSW	5	14,719,009 (GRCm39)	missense	unknown
R3719:Pclo	UTSW	5	14,571,175 (GRCm39)	missense	probably benign	0.33
R3739:Pclo	UTSW	5	14,730,913 (GRCm39)	missense	unknown
R3745:Pclo	UTSW	5	14,728,435 (GRCm39)	unclassified	probably benign
R3771:Pclo	UTSW	5	14,589,422 (GRCm39)	critical splice acceptor site	probably null
R3789:Pclo	UTSW	5	14,730,464 (GRCm39)	unclassified	probably benign
R3803:Pclo	UTSW	5	14,565,416 (GRCm39)	nonsense	probably null
R3902:Pclo	UTSW	5	14,762,536 (GRCm39)	missense	probably benign	0.01
R3942:Pclo	UTSW	5	14,729,932 (GRCm39)	unclassified	probably benign
R4061:Pclo	UTSW	5	14,590,580 (GRCm39)	missense	unknown
R4094:Pclo	UTSW	5	14,905,659 (GRCm39)	missense	unknown
R4175:Pclo	UTSW	5	14,763,889 (GRCm39)	missense	probably damaging	0.99
R4195:Pclo	UTSW	5	14,727,577 (GRCm39)	unclassified	probably benign
R4398:Pclo	UTSW	5	14,825,380 (GRCm39)	missense	probably damaging	1.00
R4429:Pclo	UTSW	5	14,728,114 (GRCm39)	unclassified	probably benign
R4523:Pclo	UTSW	5	14,730,006 (GRCm39)	unclassified	probably benign
R4531:Pclo	UTSW	5	14,825,422 (GRCm39)	missense	unknown
R4552:Pclo	UTSW	5	14,719,285 (GRCm39)	missense	unknown
R4563:Pclo	UTSW	5	14,571,383 (GRCm39)	missense	probably damaging	1.00
R4581:Pclo	UTSW	5	14,725,519 (GRCm39)	missense	unknown
R4638:Pclo	UTSW	5	14,730,447 (GRCm39)	nonsense	probably null
R4655:Pclo	UTSW	5	14,732,397 (GRCm39)	intron	probably benign
R4703:Pclo	UTSW	5	14,726,494 (GRCm39)	unclassified	probably benign
R4704:Pclo	UTSW	5	14,726,494 (GRCm39)	unclassified	probably benign
R4705:Pclo	UTSW	5	14,726,494 (GRCm39)	unclassified	probably benign
R4706:Pclo	UTSW	5	14,764,221 (GRCm39)	missense	unknown
R4709:Pclo	UTSW	5	14,828,572 (GRCm39)	missense	unknown
R4755:Pclo	UTSW	5	14,764,362 (GRCm39)	missense	unknown
R4786:Pclo	UTSW	5	14,773,281 (GRCm39)	missense	unknown
R4801:Pclo	UTSW	5	14,725,829 (GRCm39)	missense	unknown
R4802:Pclo	UTSW	5	14,725,829 (GRCm39)	missense	unknown
R4812:Pclo	UTSW	5	14,590,039 (GRCm39)	missense	unknown
R4817:Pclo	UTSW	5	14,763,139 (GRCm39)	missense	unknown
R4817:Pclo	UTSW	5	14,725,045 (GRCm39)	missense	unknown
R4845:Pclo	UTSW	5	14,729,132 (GRCm39)	unclassified	probably benign
R4876:Pclo	UTSW	5	14,861,694 (GRCm39)	missense	unknown
R4907:Pclo	UTSW	5	14,730,065 (GRCm39)	unclassified	probably benign
R4943:Pclo	UTSW	5	14,762,651 (GRCm39)	missense	unknown
R4963:Pclo	UTSW	5	14,719,235 (GRCm39)	missense	unknown
R4970:Pclo	UTSW	5	14,727,896 (GRCm39)	unclassified	probably benign
R4978:Pclo	UTSW	5	14,764,492 (GRCm39)	missense	probably benign	0.23
R4982:Pclo	UTSW	5	14,729,308 (GRCm39)	unclassified	probably benign
R5019:Pclo	UTSW	5	14,764,381 (GRCm39)	missense	unknown
R5068:Pclo	UTSW	5	14,729,087 (GRCm39)	unclassified	probably benign
R5092:Pclo	UTSW	5	14,727,322 (GRCm39)	unclassified	probably benign
R5112:Pclo	UTSW	5	14,727,896 (GRCm39)	unclassified	probably benign
R5124:Pclo	UTSW	5	14,727,406 (GRCm39)	unclassified	probably benign
R5210:Pclo	UTSW	5	14,763,464 (GRCm39)	missense	probably damaging	0.99
R5227:Pclo	UTSW	5	14,763,574 (GRCm39)	missense	probably benign	0.23
R5264:Pclo	UTSW	5	14,726,937 (GRCm39)	unclassified	probably benign
R5280:Pclo	UTSW	5	14,590,731 (GRCm39)	missense	unknown
R5286:Pclo	UTSW	5	14,729,761 (GRCm39)	unclassified	probably benign
R5297:Pclo	UTSW	5	14,726,263 (GRCm39)	unclassified	probably benign
R5344:Pclo	UTSW	5	14,726,626 (GRCm39)	unclassified	probably benign
R5354:Pclo	UTSW	5	14,728,822 (GRCm39)	unclassified	probably benign
R5358:Pclo	UTSW	5	14,762,750 (GRCm39)	nonsense	probably null
R5363:Pclo	UTSW	5	14,719,424 (GRCm39)	missense	unknown
R5377:Pclo	UTSW	5	14,731,367 (GRCm39)	missense	unknown
R5457:Pclo	UTSW	5	14,726,157 (GRCm39)	unclassified	probably benign
R5468:Pclo	UTSW	5	14,730,966 (GRCm39)	missense	unknown
R5588:Pclo	UTSW	5	14,838,412 (GRCm39)	missense	unknown
R5836:Pclo	UTSW	5	14,728,549 (GRCm39)	unclassified	probably benign
R5865:Pclo	UTSW	5	14,764,492 (GRCm39)	missense	probably benign	0.23
R5875:Pclo	UTSW	5	14,730,614 (GRCm39)	unclassified	probably benign
R5892:Pclo	UTSW	5	14,571,185 (GRCm39)	missense	probably damaging	1.00
R5905:Pclo	UTSW	5	14,730,399 (GRCm39)	unclassified	probably benign
R5967:Pclo	UTSW	5	14,590,669 (GRCm39)	missense	unknown
R6046:Pclo	UTSW	5	14,763,302 (GRCm39)	missense	unknown
R6059:Pclo	UTSW	5	14,861,714 (GRCm39)	missense	unknown
R6092:Pclo	UTSW	5	14,727,937 (GRCm39)	unclassified	probably benign
R6119:Pclo	UTSW	5	14,727,033 (GRCm39)	unclassified	probably benign
R6221:Pclo	UTSW	5	14,725,327 (GRCm39)	missense	unknown
R6243:Pclo	UTSW	5	14,726,457 (GRCm39)	unclassified	probably benign
R6269:Pclo	UTSW	5	14,572,108 (GRCm39)	nonsense	probably null
R6303:Pclo	UTSW	5	14,727,907 (GRCm39)	unclassified	probably benign
R6304:Pclo	UTSW	5	14,727,907 (GRCm39)	unclassified	probably benign
R6453:Pclo	UTSW	5	14,726,803 (GRCm39)	unclassified	probably benign
R6460:Pclo	UTSW	5	14,729,146 (GRCm39)	unclassified	probably benign
R6497:Pclo	UTSW	5	14,843,869 (GRCm39)	missense	unknown
R6498:Pclo	UTSW	5	14,719,505 (GRCm39)	missense	unknown
R6524:Pclo	UTSW	5	14,768,883 (GRCm39)	missense	unknown
R6682:Pclo	UTSW	5	14,589,893 (GRCm39)	missense	unknown
R6823:Pclo	UTSW	5	14,727,921 (GRCm39)	unclassified	probably benign
R6830:Pclo	UTSW	5	14,731,113 (GRCm39)	missense	unknown
R6831:Pclo	UTSW	5	14,838,443 (GRCm39)	nonsense	probably null
R6965:Pclo	UTSW	5	14,731,976 (GRCm39)	intron	probably benign
R7012:Pclo	UTSW	5	14,800,493 (GRCm39)	missense	unknown
R7028:Pclo	UTSW	5	14,763,461 (GRCm39)	missense	unknown
R7030:Pclo	UTSW	5	14,726,421 (GRCm39)	missense	probably benign	0.23
R7130:Pclo	UTSW	5	14,729,356 (GRCm39)	missense	unknown
R7141:Pclo	UTSW	5	14,729,271 (GRCm39)	missense	unknown
R7143:Pclo	UTSW	5	14,908,836 (GRCm39)	missense	unknown
R7189:Pclo	UTSW	5	14,571,932 (GRCm39)	missense	possibly damaging	0.83
R7190:Pclo	UTSW	5	14,729,743 (GRCm39)	missense	unknown
R7273:Pclo	UTSW	5	14,731,608 (GRCm39)	missense	unknown
R7341:Pclo	UTSW	5	14,725,850 (GRCm39)	missense	unknown
R7361:Pclo	UTSW	5	14,843,882 (GRCm39)	missense	probably damaging	0.99
R7390:Pclo	UTSW	5	14,732,024 (GRCm39)	missense	unknown
R7396:Pclo	UTSW	5	14,589,902 (GRCm39)	missense	unknown
R7428:Pclo	UTSW	5	14,800,531 (GRCm39)	missense
R7448:Pclo	UTSW	5	14,719,631 (GRCm39)	missense	unknown
R7476:Pclo	UTSW	5	14,571,345 (GRCm39)	missense	probably damaging	1.00
R7483:Pclo	UTSW	5	14,762,606 (GRCm39)	missense
R7524:Pclo	UTSW	5	14,728,317 (GRCm39)	missense	unknown
R7526:Pclo	UTSW	5	14,571,076 (GRCm39)	missense	probably benign	0.11
R7537:Pclo	UTSW	5	14,732,118 (GRCm39)	missense	unknown
R7597:Pclo	UTSW	5	14,908,869 (GRCm39)	missense	unknown
R7597:Pclo	UTSW	5	14,727,601 (GRCm39)	missense	unknown
R7605:Pclo	UTSW	5	14,729,050 (GRCm39)	missense	unknown
R7646:Pclo	UTSW	5	14,570,909 (GRCm39)	missense	probably damaging	1.00
R7685:Pclo	UTSW	5	14,730,630 (GRCm39)	missense	unknown
R7879:Pclo	UTSW	5	14,727,228 (GRCm39)	missense	unknown
R7885:Pclo	UTSW	5	14,764,209 (GRCm39)	frame shift	probably null
R7885:Pclo	UTSW	5	14,764,205 (GRCm39)	frame shift	probably null
R7885:Pclo	UTSW	5	14,764,204 (GRCm39)	frame shift	probably null
R7887:Pclo	UTSW	5	14,764,204 (GRCm39)	frame shift	probably null
R7921:Pclo	UTSW	5	14,719,248 (GRCm39)	missense	unknown
R7948:Pclo	UTSW	5	14,815,180 (GRCm39)	nonsense	probably null
R7970:Pclo	UTSW	5	14,764,492 (GRCm39)	missense
R8021:Pclo	UTSW	5	14,589,798 (GRCm39)	missense	unknown
R8034:Pclo	UTSW	5	14,571,214 (GRCm39)	missense	probably damaging	0.99
R8079:Pclo	UTSW	5	14,590,472 (GRCm39)	missense	unknown
R8092:Pclo	UTSW	5	14,727,560 (GRCm39)	missense	unknown
R8096:Pclo	UTSW	5	14,762,975 (GRCm39)	missense
R8182:Pclo	UTSW	5	14,905,634 (GRCm39)	missense	unknown
R8188:Pclo	UTSW	5	14,725,193 (GRCm39)	missense	unknown
R8218:Pclo	UTSW	5	14,725,677 (GRCm39)	missense	unknown
R8288:Pclo	UTSW	5	14,762,885 (GRCm39)	missense
R8330:Pclo	UTSW	5	14,725,311 (GRCm39)	missense	unknown
R8382:Pclo	UTSW	5	14,727,080 (GRCm39)	missense	unknown
R8447:Pclo	UTSW	5	14,731,423 (GRCm39)	missense
R8491:Pclo	UTSW	5	14,565,244 (GRCm39)	missense	unknown
R8506:Pclo	UTSW	5	14,590,759 (GRCm39)	missense	unknown
R8524:Pclo	UTSW	5	14,729,521 (GRCm39)	unclassified	probably benign
R8548:Pclo	UTSW	5	14,732,268 (GRCm39)	splice site	probably null
R8670:Pclo	UTSW	5	14,732,061 (GRCm39)	missense	unknown
R8691:Pclo	UTSW	5	14,571,706 (GRCm39)	missense	unknown
R8719:Pclo	UTSW	5	14,763,778 (GRCm39)	nonsense	probably null
R8743:Pclo	UTSW	5	14,764,580 (GRCm39)	missense
R8756:Pclo	UTSW	5	14,764,398 (GRCm39)	missense
R8829:Pclo	UTSW	5	14,838,464 (GRCm39)	missense
R8832:Pclo	UTSW	5	14,838,464 (GRCm39)	missense
R8912:Pclo	UTSW	5	14,825,335 (GRCm39)	missense
R8930:Pclo	UTSW	5	14,762,989 (GRCm39)	missense	possibly damaging	0.95
R8932:Pclo	UTSW	5	14,762,989 (GRCm39)	missense	possibly damaging	0.95
R8958:Pclo	UTSW	5	14,763,056 (GRCm39)	missense	unknown
R8958:Pclo	UTSW	5	14,725,369 (GRCm39)	missense	unknown
R8969:Pclo	UTSW	5	14,572,208 (GRCm39)	missense	unknown
R8989:Pclo	UTSW	5	14,719,325 (GRCm39)	missense	unknown
R8991:Pclo	UTSW	5	14,719,325 (GRCm39)	missense	unknown
R8992:Pclo	UTSW	5	14,719,325 (GRCm39)	missense	unknown
R8998:Pclo	UTSW	5	14,727,510 (GRCm39)	missense	unknown
R9013:Pclo	UTSW	5	14,727,627 (GRCm39)	missense	unknown
R9035:Pclo	UTSW	5	14,763,192 (GRCm39)	missense
R9060:Pclo	UTSW	5	14,726,599 (GRCm39)	missense	unknown
R9122:Pclo	UTSW	5	14,729,998 (GRCm39)	missense	unknown
R9164:Pclo	UTSW	5	14,731,699 (GRCm39)	missense
R9170:Pclo	UTSW	5	14,731,068 (GRCm39)	missense
R9192:Pclo	UTSW	5	14,590,204 (GRCm39)	missense	unknown
R9203:Pclo	UTSW	5	14,728,528 (GRCm39)	missense	unknown
R9245:Pclo	UTSW	5	14,726,959 (GRCm39)	missense	unknown
R9260:Pclo	UTSW	5	14,764,287 (GRCm39)	missense	unknown
R9336:Pclo	UTSW	5	14,726,562 (GRCm39)	missense	unknown
R9378:Pclo	UTSW	5	14,815,877 (GRCm39)	missense
R9454:Pclo	UTSW	5	14,762,452 (GRCm39)	missense	probably benign	0.01
R9462:Pclo	UTSW	5	14,840,408 (GRCm39)	missense
R9464:Pclo	UTSW	5	14,816,830 (GRCm39)	missense
R9474:Pclo	UTSW	5	14,571,250 (GRCm39)	missense	possibly damaging	0.78
R9500:Pclo	UTSW	5	14,725,648 (GRCm39)	missense	unknown
R9528:Pclo	UTSW	5	14,727,691 (GRCm39)	missense	unknown
R9567:Pclo	UTSW	5	14,763,436 (GRCm39)	missense
R9569:Pclo	UTSW	5	14,907,065 (GRCm39)	critical splice donor site	probably null
R9578:Pclo	UTSW	5	14,816,753 (GRCm39)	missense
R9647:Pclo	UTSW	5	14,731,788 (GRCm39)	missense
R9673:Pclo	UTSW	5	14,908,758 (GRCm39)	critical splice acceptor site	probably null
R9691:Pclo	UTSW	5	14,727,558 (GRCm39)	missense	unknown
R9706:Pclo	UTSW	5	14,905,683 (GRCm39)	missense	unknown
R9803:Pclo	UTSW	5	14,762,629 (GRCm39)	missense
RF015:Pclo	UTSW	5	14,565,283 (GRCm39)	missense	unknown
X0058:Pclo	UTSW	5	14,732,145 (GRCm39)	missense	probably benign	0.23
Z1176:Pclo	UTSW	5	14,762,662 (GRCm39)	missense	probably damaging	1.00
Z1176:Pclo	UTSW	5	14,731,793 (GRCm39)	missense
Z1176:Pclo	UTSW	5	14,731,530 (GRCm39)	missense	possibly damaging	0.95
Z1177:Pclo	UTSW	5	14,572,027 (GRCm39)	missense	possibly damaging	0.68
Z1177:Pclo	UTSW	5	14,572,022 (GRCm39)	missense	probably damaging	0.99
Z1177:Pclo	UTSW	5	14,728,170 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TACTGTAGAGGCCACAGCTG -3'
(R):5'- TGTTGGAGTCCCAGGTTCAG -3'

Sequencing Primer
(F):5'- CGCAGGAGCAATGGGTTACC -3'
(R):5'- TCCCAGGTTCAGAGGGCAATG -3'

Posted On

2014-07-14