Mutagenetix > Incidental Mutation

Incidental Mutation 'R1928:Dctn1'

215042

Institutional Source

Beutler Lab

Gene Symbol

Dctn1

Ensembl Gene

ENSMUSG00000031865

Gene Name

dynactin 1

Synonyms

p150, Glued, p150

MMRRC Submission

039946-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1928 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

83142902-83177099 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

C to A at 83176166 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000109552 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077407] [ENSMUST00000113907] [ENSMUST00000113913] [ENSMUST00000113918] [ENSMUST00000113919]

AlphaFold

O08788

Predicted Effect

probably benign
Transcript: ENSMUST00000077407

SMART Domains

Protein: ENSMUSP00000076623
Gene: ENSMUSG00000031865

Domain	Start	End	E-Value	Type
CAP_GLY	12	78	5.52e-31	SMART
low complexity region	124	147	N/A	INTRINSIC
low complexity region	148	177	N/A	INTRINSIC
SCOP:d1fxkc_	185	337	3e-3	SMART
low complexity region	363	379	N/A	INTRINSIC
Pfam:Dynactin	489	768	8.2e-91	PFAM
low complexity region	800	820	N/A	INTRINSIC
coiled coil region	914	1009	N/A	INTRINSIC
low complexity region	1025	1043	N/A	INTRINSIC
coiled coil region	1143	1172	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113907

SMART Domains

Protein: ENSMUSP00000109540
Gene: ENSMUSG00000031865

Domain	Start	End	E-Value	Type
low complexity region	5	22	N/A	INTRINSIC
low complexity region	27	50	N/A	INTRINSIC
low complexity region	51	80	N/A	INTRINSIC
low complexity region	93	106	N/A	INTRINSIC
low complexity region	140	158	N/A	INTRINSIC
SCOP:d1lxa__	271	345	8e-3	SMART
Pfam:Dynactin	392	671	7.1e-91	PFAM
low complexity region	703	723	N/A	INTRINSIC
coiled coil region	817	912	N/A	INTRINSIC
low complexity region	928	946	N/A	INTRINSIC
coiled coil region	1046	1075	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113913

SMART Domains

Protein: ENSMUSP00000109546
Gene: ENSMUSG00000031865

Domain	Start	End	E-Value	Type
CAP_GLY	12	78	5.52e-31	SMART
low complexity region	118	139	N/A	INTRINSIC
low complexity region	144	167	N/A	INTRINSIC
low complexity region	168	197	N/A	INTRINSIC
SCOP:d1fxkc_	205	357	3e-3	SMART
low complexity region	383	399	N/A	INTRINSIC
Pfam:Dynactin	509	788	2.5e-90	PFAM
low complexity region	820	840	N/A	INTRINSIC
coiled coil region	934	1029	N/A	INTRINSIC
low complexity region	1051	1069	N/A	INTRINSIC
coiled coil region	1168	1197	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113918

SMART Domains

Protein: ENSMUSP00000109551
Gene: ENSMUSG00000031865

Domain	Start	End	E-Value	Type
CAP_GLY	29	95	5.52e-31	SMART
low complexity region	135	156	N/A	INTRINSIC
low complexity region	161	184	N/A	INTRINSIC
low complexity region	185	214	N/A	INTRINSIC
low complexity region	227	240	N/A	INTRINSIC
low complexity region	274	292	N/A	INTRINSIC
low complexity region	400	416	N/A	INTRINSIC
Pfam:Dynactin	526	805	3.3e-90	PFAM
low complexity region	837	857	N/A	INTRINSIC
coiled coil region	951	1046	N/A	INTRINSIC
coiled coil region	1147	1176	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113919

SMART Domains

Protein: ENSMUSP00000109552
Gene: ENSMUSG00000031865

Domain	Start	End	E-Value	Type
CAP_GLY	29	95	5.52e-31	SMART
low complexity region	135	156	N/A	INTRINSIC
low complexity region	161	184	N/A	INTRINSIC
low complexity region	185	214	N/A	INTRINSIC
SCOP:d1fxkc_	222	374	3e-3	SMART
low complexity region	400	416	N/A	INTRINSIC
Pfam:Dynactin	522	805	1.4e-103	PFAM
low complexity region	837	857	N/A	INTRINSIC
coiled coil region	951	1046	N/A	INTRINSIC
low complexity region	1068	1086	N/A	INTRINSIC
coiled coil region	1185	1214	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127824

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130917

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154420

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153793

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.6%

Validation Efficiency

98% (120/123)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality and developmental arrest at E7.5 associated with increased apoptosis. [provided by MGI curators]

Allele List at MGI

All alleles(20) : Targeted(4) Gene trapped(16)

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630073D07Rik	T	G	6: 132,603,564 (GRCm39)	Q64P	unknown	Het
Adgrv1	A	T	13: 81,668,905 (GRCm39)	F1830L	probably benign	Het
Anks3	C	T	16: 4,763,918 (GRCm39)		probably null	Het
Aqp12	G	A	1: 92,934,332 (GRCm39)	D70N	probably damaging	Het
B4galnt4	C	T	7: 140,648,061 (GRCm39)	R526*	probably null	Het
Best2	A	G	8: 85,737,882 (GRCm39)	F171S	probably benign	Het
C2cd5	A	T	6: 142,958,956 (GRCm39)	V965D	probably damaging	Het
Cd33	T	C	7: 43,179,303 (GRCm39)	E282G	probably benign	Het
Cep95	C	T	11: 106,681,554 (GRCm39)		probably benign	Het
Chd6	A	T	2: 160,809,920 (GRCm39)		probably benign	Het
Clec4d	A	T	6: 123,244,120 (GRCm39)		probably null	Het
Cntd1	C	T	11: 101,174,678 (GRCm39)	S128L	probably damaging	Het
Col19a1	T	C	1: 24,490,835 (GRCm39)		probably benign	Het
Ctrb1	C	A	8: 112,415,324 (GRCm39)	V117L	probably benign	Het
Dclk1	T	C	3: 55,154,942 (GRCm39)	V124A	possibly damaging	Het
Dcp2	T	C	18: 44,538,638 (GRCm39)		probably null	Het
Dennd11	A	G	6: 40,388,648 (GRCm39)	F238L	probably benign	Het
Ephb3	T	A	16: 21,041,045 (GRCm39)	M701K	possibly damaging	Het
Ero1b	A	G	13: 12,616,648 (GRCm39)	E359G	probably damaging	Het
Exosc8	G	T	3: 54,636,266 (GRCm39)	A255E	probably damaging	Het
Fhip1a	G	A	3: 85,595,838 (GRCm39)	P349L	probably damaging	Het
Fndc3c1	C	T	X: 105,477,128 (GRCm39)	A824T	probably benign	Het
Gja10	G	A	4: 32,601,812 (GRCm39)	Q191*	probably null	Het
Gm1587	C	T	14: 78,036,288 (GRCm39)	R6Q	unknown	Het
Gm3409	A	G	5: 146,476,418 (GRCm39)	I190V	probably benign	Het
Gm4952	G	T	19: 12,600,973 (GRCm39)	M64I	probably damaging	Het
Gramd1b	C	T	9: 40,217,765 (GRCm39)	M595I	possibly damaging	Het
Grik1	C	T	16: 87,848,241 (GRCm39)	V176M	probably damaging	Het
Grin2b	A	G	6: 136,021,044 (GRCm39)	C86R	probably damaging	Het
Gtse1	C	T	15: 85,746,264 (GRCm39)		probably benign	Het
Hectd2	T	C	19: 36,589,719 (GRCm39)	Y615H	probably damaging	Het
Hydin	T	C	8: 111,229,579 (GRCm39)	F1552S	possibly damaging	Het
Ifi207	A	G	1: 173,557,211 (GRCm39)	V516A	possibly damaging	Het
Igfbp5	G	T	1: 72,913,184 (GRCm39)	P39T	probably damaging	Het
Il23r	T	A	6: 67,400,719 (GRCm39)	D537V	possibly damaging	Het
Invs	A	G	4: 48,390,095 (GRCm39)	Y251C	probably damaging	Het
Isl1	G	T	13: 116,444,953 (GRCm39)	H25Q	probably damaging	Het
Kif13a	A	G	13: 46,966,221 (GRCm39)	L399P	probably damaging	Het
Klhl1	A	G	14: 96,584,225 (GRCm39)	V335A	probably benign	Het
Klhl14	C	A	18: 21,784,843 (GRCm39)	A195S	probably damaging	Het
Lepr	A	G	4: 101,639,927 (GRCm39)		probably benign	Het
Map1b	A	C	13: 99,567,454 (GRCm39)	S1756A	unknown	Het
Med23	C	T	10: 24,785,710 (GRCm39)	A877V	probably benign	Het
Mfsd4b2	T	C	10: 39,797,458 (GRCm39)	Y299C	probably damaging	Het
Mipol1	T	A	12: 57,379,205 (GRCm39)	M221K	probably damaging	Het
Muc4	A	T	16: 32,569,350 (GRCm39)	S137C	probably damaging	Het
Mutyh	T	C	4: 116,673,855 (GRCm39)	Y189H	probably damaging	Het
Nabp2	T	C	10: 128,245,182 (GRCm39)	T21A	possibly damaging	Het
Nalf1	T	C	8: 9,820,217 (GRCm39)	T268A	probably benign	Het
Nell1	T	A	7: 50,350,943 (GRCm39)	V530E	possibly damaging	Het
Npc1	G	C	18: 12,346,435 (GRCm39)	P254A	possibly damaging	Het
Nup153	A	G	13: 46,854,502 (GRCm39)	V530A	probably damaging	Het
Nxpe2	T	C	9: 48,237,914 (GRCm39)	T114A	probably damaging	Het
Or10j5	T	C	1: 172,784,881 (GRCm39)	V173A	probably damaging	Het
Or11h4b	A	G	14: 50,918,872 (GRCm39)	V73A	probably benign	Het
Or7e177	T	C	9: 20,212,354 (GRCm39)	V287A	probably benign	Het
Pabpc1l	T	A	2: 163,874,174 (GRCm39)	I193N	possibly damaging	Het
Pabpc4l	A	G	3: 46,401,066 (GRCm39)	Y193H	probably damaging	Het
Pank1	T	C	19: 34,856,281 (GRCm39)	S66G	probably benign	Het
Pcdhb1	A	T	18: 37,399,233 (GRCm39)	K395*	probably null	Het
Pcna	A	G	2: 132,093,817 (GRCm39)		probably benign	Het
Pgr	T	A	9: 8,903,630 (GRCm39)	Y550*	probably null	Het
Phf11a	A	G	14: 59,519,316 (GRCm39)		probably benign	Het
Pkhd1	A	T	1: 20,151,524 (GRCm39)		probably benign	Het
Pofut2	T	A	10: 77,096,642 (GRCm39)	Y122*	probably null	Het
Pole	A	G	5: 110,475,644 (GRCm39)	M1818V	probably benign	Het
Rapgef3	A	G	15: 97,647,914 (GRCm39)	L696P	probably damaging	Het
Rasal3	T	C	17: 32,616,327 (GRCm39)	D310G	probably damaging	Het
Rhoq	A	G	17: 87,302,486 (GRCm39)	K141E	probably benign	Het
Rilpl1	A	G	5: 124,652,813 (GRCm39)		probably benign	Het
Rnaseh1	T	C	12: 28,703,088 (GRCm39)	S91P	probably benign	Het
Rsf1	G	GACGGCGGCT	7: 97,229,116 (GRCm39)		probably benign	Het
Saa1	C	A	7: 46,391,856 (GRCm39)	G31W	probably null	Het
Saxo5	T	A	8: 3,536,947 (GRCm39)	I431K	possibly damaging	Het
Scaf8	A	G	17: 3,218,352 (GRCm39)	T241A	unknown	Het
Scarb2	C	T	5: 92,592,125 (GRCm39)	A473T	possibly damaging	Het
Scnn1b	T	C	7: 121,509,670 (GRCm39)	F273S	probably damaging	Het
Sdccag8	T	C	1: 176,656,536 (GRCm39)	V136A	probably damaging	Het
Sdr16c6	A	T	4: 4,069,926 (GRCm39)	V138E	probably damaging	Het
Sf3a3	G	A	4: 124,615,886 (GRCm39)	A180T	possibly damaging	Het
Slc16a5	T	A	11: 115,360,842 (GRCm39)	S342T	probably damaging	Het
Slc18a1	A	G	8: 69,526,464 (GRCm39)	S75P	probably benign	Het
Slc7a6	T	A	8: 106,920,120 (GRCm39)		probably benign	Het
Smco3	G	A	6: 136,808,845 (GRCm39)	Q10*	probably null	Het
Spem2	T	C	11: 69,708,290 (GRCm39)	Q225R	probably benign	Het
Ssb	G	T	2: 69,697,901 (GRCm39)		probably null	Het
Stx8	T	C	11: 68,000,106 (GRCm39)	I182T	probably damaging	Het
Synj2	T	A	17: 6,040,542 (GRCm39)	I121N	probably damaging	Het
Tbc1d1	T	C	5: 64,502,643 (GRCm39)	Y1055H	probably damaging	Het
Thbs3	G	T	3: 89,125,067 (GRCm39)	R52L	probably damaging	Het
Tmem101	C	T	11: 102,044,222 (GRCm39)	V222I	probably benign	Het
Tnks2	C	T	19: 36,823,068 (GRCm39)	Q112*	probably null	Het
Tnrc6b	T	A	15: 80,764,924 (GRCm39)	W809R	probably damaging	Het
Trib2	T	A	12: 15,865,454 (GRCm39)	R16S	probably damaging	Het
Trim12a	T	C	7: 103,956,331 (GRCm39)	N70D	probably damaging	Het
Trim55	T	A	3: 19,716,046 (GRCm39)		probably null	Het
Tspyl5	T	A	15: 33,687,153 (GRCm39)	D264V	probably damaging	Het
Ttn	A	G	2: 76,555,388 (GRCm39)	V30539A	probably damaging	Het
Tubgcp3	A	G	8: 12,713,988 (GRCm39)	F43S	possibly damaging	Het
Tut7	A	G	13: 59,964,548 (GRCm39)	L209P	probably damaging	Het
Ube3d	A	G	9: 86,305,056 (GRCm39)	L262P	probably damaging	Het
Ugt1a7c	T	C	1: 88,023,651 (GRCm39)	V270A	probably benign	Het
Vav3	T	A	3: 109,413,738 (GRCm39)	F225L	possibly damaging	Het
Vmn1r21	G	T	6: 57,821,077 (GRCm39)	Y122*	probably null	Het
Vmn1r225	C	A	17: 20,723,071 (GRCm39)	Q171K	probably benign	Het
Vmn2r103	T	C	17: 20,032,029 (GRCm39)	V601A	possibly damaging	Het
Vmn2r19	T	A	6: 123,308,589 (GRCm39)	N555K	probably damaging	Het
Vps33a	G	A	5: 123,696,684 (GRCm39)	A323V	probably benign	Het
Wdr77	C	T	3: 105,874,618 (GRCm39)	P337S	probably benign	Het
Wnk1	A	T	6: 119,929,884 (GRCm39)	I93N	probably damaging	Het
Zbtb9	T	A	17: 27,193,612 (GRCm39)	I339N	probably damaging	Het
Zfp202	T	A	9: 40,121,083 (GRCm39)	D241E	probably damaging	Het
Zfp235	T	A	7: 23,840,563 (GRCm39)	Y327*	probably null	Het
Zfp820	A	C	17: 22,038,316 (GRCm39)	D337E	probably benign	Het
Zfp944	T	C	17: 22,560,065 (GRCm39)	T14A	probably damaging	Het
Zfy1	A	G	Y: 729,733 (GRCm39)	V303A	unknown	Het
Zfyve26	C	T	12: 79,286,744 (GRCm39)	W2281*	probably null	Het

Other mutations in Dctn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01113:Dctn1	APN	6	83,156,879 (GRCm39)	missense	probably benign	0.00
IGL01450:Dctn1	APN	6	83,171,092 (GRCm39)	unclassified	probably benign
IGL01876:Dctn1	APN	6	83,174,903 (GRCm39)	missense	probably damaging	1.00
IGL01958:Dctn1	APN	6	83,168,326 (GRCm39)	missense	possibly damaging	0.95
IGL02554:Dctn1	APN	6	83,159,704 (GRCm39)	missense	probably damaging	1.00
IGL02668:Dctn1	APN	6	83,168,030 (GRCm39)	missense	possibly damaging	0.89
IGL02814:Dctn1	APN	6	83,166,896 (GRCm39)	missense	probably damaging	1.00
IGL02818:Dctn1	APN	6	83,169,496 (GRCm39)	missense	possibly damaging	0.86
IGL03007:Dctn1	APN	6	83,159,690 (GRCm39)	missense	probably damaging	1.00
IGL03065:Dctn1	APN	6	83,169,475 (GRCm39)	missense	probably damaging	0.99
IGL03083:Dctn1	APN	6	83,174,466 (GRCm39)	splice site	probably benign
IGL03394:Dctn1	APN	6	83,168,266 (GRCm39)	missense	possibly damaging	0.61
E0374:Dctn1	UTSW	6	83,171,156 (GRCm39)	missense	possibly damaging	0.93
IGL03014:Dctn1	UTSW	6	83,174,351 (GRCm39)	intron	probably benign
PIT4812001:Dctn1	UTSW	6	83,176,744 (GRCm39)	missense	possibly damaging	0.86
R0044:Dctn1	UTSW	6	83,168,116 (GRCm39)	missense	probably damaging	1.00
R0047:Dctn1	UTSW	6	83,159,614 (GRCm39)	nonsense	probably null
R0047:Dctn1	UTSW	6	83,159,614 (GRCm39)	nonsense	probably null
R0057:Dctn1	UTSW	6	83,156,874 (GRCm39)	missense	probably benign	0.14
R0731:Dctn1	UTSW	6	83,160,071 (GRCm39)	missense	probably damaging	0.98
R0738:Dctn1	UTSW	6	83,167,089 (GRCm39)	critical splice donor site	probably null
R0755:Dctn1	UTSW	6	83,166,059 (GRCm39)	missense	probably damaging	0.96
R0839:Dctn1	UTSW	6	83,167,459 (GRCm39)	missense	possibly damaging	0.53
R1035:Dctn1	UTSW	6	83,167,202 (GRCm39)	missense	probably damaging	1.00
R1454:Dctn1	UTSW	6	83,174,490 (GRCm39)	missense	possibly damaging	0.93
R1469:Dctn1	UTSW	6	83,169,871 (GRCm39)	missense	probably damaging	1.00
R1469:Dctn1	UTSW	6	83,169,871 (GRCm39)	missense	probably damaging	1.00
R1627:Dctn1	UTSW	6	83,172,064 (GRCm39)	missense	probably damaging	0.99
R1631:Dctn1	UTSW	6	83,174,578 (GRCm39)	missense	possibly damaging	0.56
R1812:Dctn1	UTSW	6	83,169,500 (GRCm39)	missense	possibly damaging	0.85
R2008:Dctn1	UTSW	6	83,166,938 (GRCm39)	missense	probably damaging	0.99
R2242:Dctn1	UTSW	6	83,176,687 (GRCm39)	missense	probably damaging	0.99
R2259:Dctn1	UTSW	6	83,174,568 (GRCm39)	missense	possibly damaging	0.46
R2422:Dctn1	UTSW	6	83,176,782 (GRCm39)	missense	possibly damaging	0.92
R2483:Dctn1	UTSW	6	83,171,169 (GRCm39)	missense	probably damaging	1.00
R4455:Dctn1	UTSW	6	83,172,031 (GRCm39)	missense	probably damaging	1.00
R4724:Dctn1	UTSW	6	83,166,920 (GRCm39)	missense	possibly damaging	0.53
R4812:Dctn1	UTSW	6	83,166,919 (GRCm39)	missense	probably benign	0.24
R4819:Dctn1	UTSW	6	83,167,501 (GRCm39)	missense	probably damaging	0.97
R4831:Dctn1	UTSW	6	83,176,753 (GRCm39)	missense	possibly damaging	0.46
R4928:Dctn1	UTSW	6	83,166,189 (GRCm39)	missense	possibly damaging	0.73
R5087:Dctn1	UTSW	6	83,168,621 (GRCm39)	missense	probably damaging	1.00
R5354:Dctn1	UTSW	6	83,160,108 (GRCm39)	missense	possibly damaging	0.93
R5372:Dctn1	UTSW	6	83,167,192 (GRCm39)	missense	probably damaging	0.96
R5493:Dctn1	UTSW	6	83,159,546 (GRCm39)	missense	possibly damaging	0.89
R5494:Dctn1	UTSW	6	83,159,546 (GRCm39)	missense	possibly damaging	0.89
R5732:Dctn1	UTSW	6	83,174,931 (GRCm39)	critical splice donor site	probably null
R5856:Dctn1	UTSW	6	83,174,847 (GRCm39)	missense	probably damaging	1.00
R6025:Dctn1	UTSW	6	83,170,673 (GRCm39)	splice site	probably null
R6999:Dctn1	UTSW	6	83,168,263 (GRCm39)	missense	possibly damaging	0.89
R7052:Dctn1	UTSW	6	83,172,262 (GRCm39)	splice site	probably null
R7133:Dctn1	UTSW	6	83,157,026 (GRCm39)	splice site	probably null
R7485:Dctn1	UTSW	6	83,166,887 (GRCm39)	missense	possibly damaging	0.85
R7607:Dctn1	UTSW	6	83,172,051 (GRCm39)	nonsense	probably null
R7729:Dctn1	UTSW	6	83,160,042 (GRCm39)	missense	probably damaging	1.00
R7749:Dctn1	UTSW	6	83,163,123 (GRCm39)	intron	probably benign
R8282:Dctn1	UTSW	6	83,176,738 (GRCm39)	missense	possibly damaging	0.91
R8750:Dctn1	UTSW	6	83,160,108 (GRCm39)	missense	possibly damaging	0.93
R9126:Dctn1	UTSW	6	83,169,835 (GRCm39)	missense	probably damaging	0.99
R9208:Dctn1	UTSW	6	83,176,684 (GRCm39)	missense	probably benign	0.33
R9422:Dctn1	UTSW	6	83,170,691 (GRCm39)	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- GCTGCAGTGTCTGTCAAGTG -3'
(R):5'- TCTTCAACCCCAAGAGGAAGG -3'

Sequencing Primer
(F):5'- GCCAGTGGCTTCCTAGAAG -3'
(R):5'- GGGAGAGGCCACAGGAGC -3'

Posted On

2014-07-14