Mutagenetix > Incidental Mutation

Incidental Mutation 'R1934:Birc2'

215697

Institutional Source

Beutler Lab

Gene Symbol

Birc2

Ensembl Gene

ENSMUSG00000057367

Gene Name

baculoviral IAP repeat-containing 2

Synonyms

cIAP1, Api1, HIAP1, cIAP-1, MIAP1, mcIAP1, MIHB, IAP1

MMRRC Submission

039952-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.906) question?

Stock #

R1934 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

7818228-7837065 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 7854500 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 397 (T397A)

Ref Sequence

ENSEMBL: ENSMUSP00000013949 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000013949] [ENSMUST00000115672]

AlphaFold

Q62210

Predicted Effect

possibly damaging
Transcript: ENSMUST00000013949
AA Change: T397A

PolyPhen 2 Score 0.890 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000013949
Gene: ENSMUSG00000032000
AA Change: T397A

Domain	Start	End	E-Value	Type
BIR	27	98	1.92e-34	SMART
low complexity region	132	145	N/A	INTRINSIC
BIR	167	237	2.12e-36	SMART
BIR	253	324	1.5e-36	SMART
CARD	438	525	7.29e-4	SMART
RING	555	589	1.43e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115672

SMART Domains

Protein: ENSMUSP00000111336
Gene: ENSMUSG00000032000

Domain	Start	End	E-Value	Type
BIR	27	98	1.92e-34	SMART
low complexity region	132	145	N/A	INTRINSIC
BIR	167	237	2.12e-36	SMART
BIR	253	324	1.5e-36	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000161642
AA Change: T82A

SMART Domains

Protein: ENSMUSP00000125512
Gene: ENSMUSG00000032000
AA Change: T82A

Domain	Start	End	E-Value	Type
SCOP:d1g73d_	4	35	1e-6	SMART
PDB:3T6P\|A	4	106	2e-39	PDB
low complexity region	107	125	N/A	INTRINSIC

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.3%
20x: 92.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
PHENOTYPE: Homozygous null mice showed a modest reduction in the number of lymphocytes. Mice homozygous for a knock-in allele exhibit increased T cell poliferation and IFNG secretion in response to anti-CD3 stimulation. [provided by MGI curators]

Allele List at MGI

All alleles(4) : Targeted, knock-out(2) Gene trapped(2)

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	T	C	6: 121,626,792 (GRCm39)	L548P	probably damaging	Het
Abca6	A	G	11: 110,100,909 (GRCm39)		probably null	Het
Abcb5	A	G	12: 118,871,235 (GRCm39)		probably null	Het
Acacb	G	A	5: 114,336,343 (GRCm39)	A686T	probably benign	Het
Acot6	T	C	12: 84,153,367 (GRCm39)	V203A	probably benign	Het
Adam25	A	T	8: 41,207,922 (GRCm39)	Y396F	probably benign	Het
Adamts9	A	G	6: 92,920,102 (GRCm39)	L12P	possibly damaging	Het
Adamtsl2	A	T	2: 26,979,605 (GRCm39)	D258V	probably damaging	Het
Aox4	G	A	1: 58,285,095 (GRCm39)	V616I	probably benign	Het
Ap1m1	A	G	8: 73,009,637 (GRCm39)	I382V	probably damaging	Het
Arhgef2	G	A	3: 88,537,098 (GRCm39)	R8Q	probably damaging	Het
Arhgef37	T	G	18: 61,657,014 (GRCm39)	E17A	probably benign	Het
Arhgef7	C	A	8: 11,858,713 (GRCm39)		probably null	Het
Arih1	T	C	9: 59,302,215 (GRCm39)	D431G	probably damaging	Het
Astn2	C	T	4: 65,353,426 (GRCm39)	V1115M	probably damaging	Het
Atg7	T	A	6: 114,678,196 (GRCm39)	M280K	probably damaging	Het
Ccdc110	A	G	8: 46,396,287 (GRCm39)	N726S	probably damaging	Het
Cdadc1	A	T	14: 59,827,309 (GRCm39)	S121T	possibly damaging	Het
Cdh16	A	G	8: 105,344,595 (GRCm39)	V7A	possibly damaging	Het
Cenpb	A	G	2: 131,021,184 (GRCm39)	S205P	probably benign	Het
Chsy1	T	C	7: 65,821,991 (GRCm39)	V742A	probably damaging	Het
Col12a1	G	A	9: 79,511,804 (GRCm39)	R133*	probably null	Het
Col18a1	A	C	10: 76,948,578 (GRCm39)	S311R	possibly damaging	Het
Col9a3	G	A	2: 180,248,927 (GRCm39)	V260M	probably damaging	Het
Coq2	T	A	5: 100,809,731 (GRCm39)	R17S	probably damaging	Het
Ctf1	A	G	7: 127,311,936 (GRCm39)	R4G	probably damaging	Het
Cwc27	T	C	13: 104,768,184 (GRCm39)	D437G	probably benign	Het
Cyp4f14	T	C	17: 33,125,289 (GRCm39)	N430D	probably damaging	Het
Dars2	A	G	1: 160,890,811 (GRCm39)		probably null	Het
Dgkz	A	T	2: 91,767,449 (GRCm39)	M848K	possibly damaging	Het
Dnhd1	T	G	7: 105,357,789 (GRCm39)	V3208G	probably benign	Het
Dsg1b	A	G	18: 20,528,963 (GRCm39)	Y233C	probably damaging	Het
Edem3	A	T	1: 151,680,034 (GRCm39)	D460V	probably damaging	Het
Ednra	A	G	8: 78,415,747 (GRCm39)	S167P	possibly damaging	Het
Eif3m	A	T	2: 104,831,624 (GRCm39)	V180D	probably damaging	Het
Fam217a	T	A	13: 35,094,864 (GRCm39)	R207S	probably damaging	Het
Fcgbp	G	A	7: 27,806,518 (GRCm39)	G2162D	probably damaging	Het
Fhod3	A	T	18: 25,223,335 (GRCm39)	I894F	probably benign	Het
Fpgs	A	T	2: 32,577,993 (GRCm39)	I143N	probably damaging	Het
Fpr-rs6	A	G	17: 20,403,152 (GRCm39)	S70P	probably benign	Het
Frs2	T	C	10: 116,914,806 (GRCm39)	M38V	probably damaging	Het
Fsip2	A	G	2: 82,810,902 (GRCm39)	N2407S	possibly damaging	Het
Gabrg2	T	A	11: 41,811,297 (GRCm39)	T283S	probably benign	Het
Gas2l1	T	A	11: 5,011,408 (GRCm39)	T474S	probably benign	Het
Gli1	A	T	10: 127,167,108 (GRCm39)	M715K	possibly damaging	Het
Glra3	G	T	8: 56,393,942 (GRCm39)	A18S	probably benign	Het
Gm5800	T	A	14: 51,949,396 (GRCm39)	N183I	possibly damaging	Het
Gm8674	T	A	13: 50,055,471 (GRCm39)		noncoding transcript	Het
Gnat3	A	T	5: 18,224,508 (GRCm39)	I303F	possibly damaging	Het
Grin2d	C	T	7: 45,506,251 (GRCm39)	V547M	probably damaging	Het
Grpr	C	A	X: 162,332,137 (GRCm39)	V53L	probably benign	Het
Heatr5b	T	C	17: 79,103,347 (GRCm39)	I1169V	possibly damaging	Het
Icam5	A	G	9: 20,946,082 (GRCm39)	T305A	probably benign	Het
Itga11	T	A	9: 62,651,796 (GRCm39)	N309K	probably damaging	Het
Itgb6	T	C	2: 60,499,493 (GRCm39)	D100G	probably benign	Het
Kdm3b	A	G	18: 34,946,597 (GRCm39)	K862R	probably benign	Het
Kif7	T	C	7: 79,361,286 (GRCm39)	D135G	probably benign	Het
Lrp4	A	G	2: 91,310,777 (GRCm39)	D606G	probably damaging	Het
Lrrtm1	A	T	6: 77,221,949 (GRCm39)		probably null	Het
Metap1d	A	T	2: 71,352,927 (GRCm39)	H252L	possibly damaging	Het
Mfhas1	A	G	8: 36,058,251 (GRCm39)	K909E	possibly damaging	Het
Mrgprh	C	T	17: 13,095,838 (GRCm39)	T26I	probably damaging	Het
Myo15b	T	C	11: 115,754,310 (GRCm39)	S937P	probably benign	Het
Neto2	A	T	8: 86,397,033 (GRCm39)	I73N	possibly damaging	Het
Nod2	T	C	8: 89,390,347 (GRCm39)	I196T	probably benign	Het
Nvl	T	G	1: 180,926,693 (GRCm39)	T788P	probably damaging	Het
Or13a25	C	A	7: 140,247,951 (GRCm39)	C243*	probably null	Het
Or2h2	A	T	17: 37,396,906 (GRCm39)	D50E	possibly damaging	Het
Pax3	G	A	1: 78,080,117 (GRCm39)	T423I	possibly damaging	Het
Pde4dip	A	G	3: 97,600,007 (GRCm39)	V2403A	possibly damaging	Het
Phip	C	T	9: 82,785,235 (GRCm39)	V827I	probably benign	Het
Plcb3	A	G	19: 6,941,977 (GRCm39)	F285L	probably damaging	Het
Pnp2	A	G	14: 51,193,675 (GRCm39)	I16V	probably benign	Het
Pola2	A	G	19: 6,003,769 (GRCm39)	L202P	probably damaging	Het
Ppfia1	A	T	7: 144,058,847 (GRCm39)	N651K	probably benign	Het
Prss53	T	C	7: 127,485,920 (GRCm39)		probably null	Het
Rasgrf1	G	T	9: 89,835,966 (GRCm39)	Q231H	probably damaging	Het
Rasgrf2	A	T	13: 92,131,825 (GRCm39)		probably null	Het
Rccd1	T	A	7: 79,970,272 (GRCm39)	N115I	possibly damaging	Het
Rpgrip1	A	T	14: 52,352,101 (GRCm39)	T26S	possibly damaging	Het
Rpn1	T	A	6: 88,070,841 (GRCm39)	V237E	probably damaging	Het
Sema4f	G	T	6: 82,907,908 (GRCm39)	P180Q	probably damaging	Het
Slc36a4	T	C	9: 15,632,085 (GRCm39)	V87A	probably damaging	Het
Slc5a4b	A	T	10: 75,917,307 (GRCm39)	V243E	possibly damaging	Het
Sos2	T	G	12: 69,695,315 (GRCm39)	I141L	probably damaging	Het
Srbd1	A	G	17: 86,410,321 (GRCm39)	V537A	probably damaging	Het
Sugp1	A	G	8: 70,509,225 (GRCm39)	E166G	possibly damaging	Het
Tktl2	G	A	8: 66,964,999 (GRCm39)	V186M	probably damaging	Het
Tshr	T	C	12: 91,503,955 (GRCm39)	S298P	probably damaging	Het
Tspan14	T	C	14: 40,656,209 (GRCm39)	Y6C	probably damaging	Het
Ttn	A	T	2: 76,577,384 (GRCm39)	V24503E	probably damaging	Het
Vcan	T	A	13: 89,851,045 (GRCm39)	N1305I	probably damaging	Het
Vmn2r81	A	G	10: 79,083,628 (GRCm39)	M1V	probably null	Het
Vps39	A	T	2: 120,148,558 (GRCm39)	V873E	probably damaging	Het
Vstm2a	A	T	11: 16,359,734 (GRCm39)	D270V	unknown	Het
Wdr24	A	T	17: 26,043,240 (GRCm39)	M21L	possibly damaging	Het
Wee1	A	G	7: 109,721,698 (GRCm39)	T48A	probably benign	Het
Zfp369	T	C	13: 65,444,965 (GRCm39)	C703R	probably damaging	Het
Zfp532	A	T	18: 65,818,682 (GRCm39)	D1114V	probably damaging	Het
Zfp758	A	G	17: 22,592,633 (GRCm39)	T46A	probably damaging	Het

Other mutations in Birc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00909:Birc2	APN	9	7,833,666 (GRCm39)	missense	probably damaging	1.00
IGL00972:Birc2	APN	9	7,833,716 (GRCm39)	missense	probably benign	0.36
IGL01329:Birc2	APN	9	7,860,733 (GRCm39)	missense	probably damaging	1.00
IGL02852:Birc2	APN	9	7,854,484 (GRCm39)	missense	probably damaging	0.99
IGL02888:Birc2	APN	9	7,819,559 (GRCm39)	missense	probably benign	0.22
IGL03135:Birc2	APN	9	7,849,722 (GRCm39)	splice site	probably benign
IGL03399:Birc2	APN	9	7,821,088 (GRCm39)	missense	probably damaging	1.00
1mM(1):Birc2	UTSW	9	7,819,409 (GRCm39)	missense	probably damaging	0.98
R0409:Birc2	UTSW	9	7,819,385 (GRCm39)	missense	possibly damaging	0.61
R0478:Birc2	UTSW	9	7,860,348 (GRCm39)	missense	probably damaging	1.00
R0905:Birc2	UTSW	9	7,851,052 (GRCm39)	makesense	probably null
R1617:Birc2	UTSW	9	7,826,952 (GRCm39)	missense	possibly damaging	0.66
R1864:Birc2	UTSW	9	7,819,518 (GRCm39)	missense	probably benign	0.06
R2005:Birc2	UTSW	9	7,860,342 (GRCm39)	missense	probably damaging	1.00
R2156:Birc2	UTSW	9	7,826,939 (GRCm39)	missense	probably damaging	1.00
R2519:Birc2	UTSW	9	7,821,180 (GRCm39)	missense	possibly damaging	0.46
R2867:Birc2	UTSW	9	7,834,478 (GRCm39)	start codon destroyed	probably null	0.08
R2867:Birc2	UTSW	9	7,834,478 (GRCm39)	start codon destroyed	probably null	0.08
R3019:Birc2	UTSW	9	7,857,390 (GRCm39)	critical splice donor site	probably null
R4028:Birc2	UTSW	9	7,819,352 (GRCm39)	missense	probably benign	0.01
R4169:Birc2	UTSW	9	7,849,684 (GRCm39)	missense	possibly damaging	0.78
R4243:Birc2	UTSW	9	7,834,386 (GRCm39)	missense	probably benign	0.00
R4250:Birc2	UTSW	9	7,818,936 (GRCm39)	missense	probably benign	0.00
R4584:Birc2	UTSW	9	7,833,675 (GRCm39)	missense	probably damaging	1.00
R4952:Birc2	UTSW	9	7,836,741 (GRCm39)	missense	probably damaging	0.99
R4964:Birc2	UTSW	9	7,860,553 (GRCm39)	missense	probably benign	0.01
R5017:Birc2	UTSW	9	7,818,886 (GRCm39)	nonsense	probably null
R5338:Birc2	UTSW	9	7,857,360 (GRCm39)	missense	probably benign	0.01
R5395:Birc2	UTSW	9	7,861,175 (GRCm39)	missense	probably damaging	1.00
R5877:Birc2	UTSW	9	7,849,347 (GRCm39)	missense	probably damaging	1.00
R5914:Birc2	UTSW	9	7,857,343 (GRCm39)	makesense	probably null
R6148:Birc2	UTSW	9	7,849,684 (GRCm39)	missense	possibly damaging	0.78
R6163:Birc2	UTSW	9	7,819,036 (GRCm39)	missense	probably benign	0.15
R6471:Birc2	UTSW	9	7,857,421 (GRCm39)	missense	probably benign	0.00
R6747:Birc2	UTSW	9	7,860,262 (GRCm39)	critical splice donor site	probably null
R6752:Birc2	UTSW	9	7,857,345 (GRCm39)	missense	probably benign	0.10
R6789:Birc2	UTSW	9	7,836,966 (GRCm39)	intron	probably benign
R6795:Birc2	UTSW	9	7,833,873 (GRCm39)	missense	possibly damaging	0.81
R6812:Birc2	UTSW	9	7,854,418 (GRCm39)	missense	probably damaging	0.99
R6941:Birc2	UTSW	9	7,819,469 (GRCm39)	missense	probably benign	0.00
R7105:Birc2	UTSW	9	7,819,442 (GRCm39)	missense	probably damaging	1.00
R7135:Birc2	UTSW	9	7,818,762 (GRCm39)	missense	probably damaging	1.00
R7233:Birc2	UTSW	9	7,827,009 (GRCm39)	missense	probably damaging	1.00
R7460:Birc2	UTSW	9	7,818,762 (GRCm39)	missense	probably damaging	1.00
R7517:Birc2	UTSW	9	7,819,424 (GRCm39)	missense	probably benign	0.00
R8078:Birc2	UTSW	9	7,858,742 (GRCm39)	missense	probably damaging	0.98
R8101:Birc2	UTSW	9	7,861,005 (GRCm39)	missense	probably benign	0.00
R8146:Birc2	UTSW	9	7,818,797 (GRCm39)	missense	probably damaging	1.00
R8314:Birc2	UTSW	9	7,872,942 (GRCm39)	intron	probably benign
R8371:Birc2	UTSW	9	7,849,427 (GRCm39)	missense	probably damaging	1.00
R8396:Birc2	UTSW	9	7,834,301 (GRCm39)	missense	probably benign	0.05
R9009:Birc2	UTSW	9	7,833,937 (GRCm39)	missense	probably benign
R9497:Birc2	UTSW	9	7,861,028 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGCACATCTCTCCAGGGAAAG -3'
(R):5'- ATTCTAGTGGAATGCCTGGGC -3'

Sequencing Primer
(F):5'- TCCAGGGAAAGGCCTTGTTACTC -3'
(R):5'- CTAGACTGGCCTAGAAGGATTCC -3'

Posted On

2014-07-14