Mutagenetix > Incidental Mutation

Incidental Mutation 'R1964:Dcp2'

217162

Institutional Source

Beutler Lab

Gene Symbol

Dcp2

Ensembl Gene

ENSMUSG00000024472

Gene Name

decapping mRNA 2

Synonyms

2410015D23Rik, 5730537H01Rik

MMRRC Submission

039977-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.957) question?

Stock #

R1964 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

44513569-44558036 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 44529038 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 51 (M51T)

Ref Sequence

ENSEMBL: ENSMUSP00000144010 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025350] [ENSMUST00000202306]

AlphaFold

Q9CYC6

Predicted Effect

probably benign
Transcript: ENSMUST00000025350
AA Change: M51T

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000025350
Gene: ENSMUSG00000024472
AA Change: M51T

Domain	Start	End	E-Value	Type
DCP2	10	94	4.23e-50	SMART
Pfam:NUDIX	97	219	6.5e-17	PFAM
low complexity region	240	258	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000202306
AA Change: M51T

PolyPhen 2 Score 0.495 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000144010
Gene: ENSMUSG00000024472
AA Change: M51T

Domain	Start	End	E-Value	Type
DCP2	10	82	5.7e-28	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]
PHENOTYPE: Mice homozygous for a gene trapped allele are viable and fertile. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 96 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	C	T	17: 9,211,324 (GRCm39)	H158Y	probably damaging	Het
1810009J06Rik	T	A	6: 40,945,141 (GRCm39)	C207S	probably damaging	Het
Aadac	T	A	3: 59,944,759 (GRCm39)		probably null	Het
Abca6	T	A	11: 110,075,502 (GRCm39)	I1330F	probably damaging	Het
Adar	A	T	3: 89,653,202 (GRCm39)	S263C	probably benign	Het
Adra2b	T	C	2: 127,205,734 (GRCm39)	Y84H	probably damaging	Het
Arnt2	A	T	7: 83,992,997 (GRCm39)	V181E	possibly damaging	Het
Atg7	T	C	6: 114,683,191 (GRCm39)	L418P	probably damaging	Het
Atp2c1	A	G	9: 105,323,322 (GRCm39)	L181P	probably damaging	Het
Awat2	G	A	X: 99,448,165 (GRCm39)	P148S	probably damaging	Het
Birc6	A	G	17: 74,941,880 (GRCm39)	M2737V	possibly damaging	Het
Ccdc81	A	G	7: 89,535,361 (GRCm39)	C292R	probably benign	Het
Cd14	T	A	18: 36,859,392 (GRCm39)	E21V	probably damaging	Het
Cdc14b	A	C	13: 64,363,351 (GRCm39)	C303W	probably damaging	Het
Cdh23	T	C	10: 60,221,001 (GRCm39)	I1248V	probably benign	Het
Ceacam1	T	C	7: 25,174,133 (GRCm39)	D174G	probably benign	Het
Cgas	T	G	9: 78,344,737 (GRCm39)	Y228S	probably damaging	Het
Chd7	A	G	4: 8,865,978 (GRCm39)	M717V	probably damaging	Het
Cib1	T	C	7: 79,882,120 (GRCm39)	T20A	possibly damaging	Het
Clec4d	A	G	6: 123,239,319 (GRCm39)	K9R	probably benign	Het
Cntnap1	C	A	11: 101,068,850 (GRCm39)	S131*	probably null	Het
Crybg1	T	C	10: 43,834,326 (GRCm39)	K1581R	probably damaging	Het
Csf2	T	G	11: 54,139,284 (GRCm39)	T100P	probably benign	Het
Csrp2	T	A	10: 110,767,894 (GRCm39)	D26E	probably benign	Het
Cul1	T	C	6: 47,479,505 (GRCm39)	V257A	probably damaging	Het
Cul4a	G	A	8: 13,186,406 (GRCm39)	M505I	possibly damaging	Het
Cul4a	T	C	8: 13,186,854 (GRCm39)	M530T	probably benign	Het
Ddx60	T	A	8: 62,401,903 (GRCm39)	C260S	probably benign	Het
Dennd2c	G	A	3: 103,073,807 (GRCm39)	R851H	probably damaging	Het
Dnah11	T	C	12: 118,106,027 (GRCm39)	E625G	possibly damaging	Het
Edem1	T	C	6: 108,821,908 (GRCm39)	W322R	probably benign	Het
Egflam	T	C	15: 7,276,586 (GRCm39)	T527A	probably damaging	Het
Ephb1	T	A	9: 101,848,322 (GRCm39)	M659L	possibly damaging	Het
Fbxl2	A	G	9: 113,818,237 (GRCm39)	I203T	probably benign	Het
Gabbr1	G	A	17: 37,359,351 (GRCm39)	G109R	probably damaging	Het
Gabra2	T	C	5: 71,171,793 (GRCm39)	I148V	possibly damaging	Het
Golga1	A	G	2: 38,937,099 (GRCm39)	V161A	probably benign	Het
Gps2	T	G	11: 69,807,246 (GRCm39)	S301A	probably benign	Het
H2-D1	T	C	17: 35,482,595 (GRCm39)	L105P	probably benign	Het
Igdcc4	T	C	9: 65,030,051 (GRCm39)	V367A	probably benign	Het
Igkv4-69	T	G	6: 69,260,782 (GRCm39)	Y115S	probably benign	Het
Itga5	A	G	15: 103,262,741 (GRCm39)	L309P	probably damaging	Het
Itih1	A	G	14: 30,651,580 (GRCm39)	Y871H	probably damaging	Het
Itpr2	T	A	6: 146,013,191 (GRCm39)	N2662I	probably damaging	Het
Kctd19	T	A	8: 106,115,102 (GRCm39)	E486D	probably damaging	Het
Kif5b	C	T	18: 6,209,059 (GRCm39)	R901Q	possibly damaging	Het
Kmt2a	G	A	9: 44,731,941 (GRCm39)	S2792F	probably benign	Het
Magi3	A	G	3: 103,927,718 (GRCm39)	V1023A	probably damaging	Het
Mapk9	C	A	11: 49,745,160 (GRCm39)	R25S	probably null	Het
Memo1	T	C	17: 74,552,003 (GRCm39)	T98A	possibly damaging	Het
Mill2	A	G	7: 18,590,529 (GRCm39)	K203R	probably damaging	Het
Mios	T	A	6: 8,215,798 (GRCm39)	H331Q	probably damaging	Het
Muc6	G	T	7: 141,226,329 (GRCm39)	S1566*	probably null	Het
Muc6	A	G	7: 141,226,330 (GRCm39)		probably benign	Het
Napsa	T	C	7: 44,231,109 (GRCm39)	F113L	probably benign	Het
Or5ac16	T	A	16: 59,022,271 (GRCm39)	I173F	possibly damaging	Het
Or9m1	A	G	2: 87,734,011 (GRCm39)	V3A	probably benign	Het
Oscp1	T	G	4: 125,977,415 (GRCm39)	V226G	possibly damaging	Het
Osgin2	G	T	4: 15,998,358 (GRCm39)	S421R	probably damaging	Het
Pclaf	A	G	9: 65,800,677 (GRCm39)	N50D	probably damaging	Het
Pdgfc	A	T	3: 81,082,292 (GRCm39)	I162F	probably benign	Het
Plag1	T	C	4: 3,903,956 (GRCm39)	T412A	probably benign	Het
Pogz	A	T	3: 94,785,504 (GRCm39)	T820S	probably benign	Het
Ptpn9	T	A	9: 56,967,196 (GRCm39)	V473D	probably damaging	Het
Qdpr	A	T	5: 45,596,660 (GRCm39)	M66K	possibly damaging	Het
Qrich1	A	G	9: 108,411,621 (GRCm39)	N382S	possibly damaging	Het
Rbbp8	T	C	18: 11,875,736 (GRCm39)	V883A	possibly damaging	Het
Rgl1	T	A	1: 152,424,855 (GRCm39)	I375F	probably damaging	Het
Rif1	C	T	2: 51,988,421 (GRCm39)	T720I	probably benign	Het
Rilp	A	T	11: 75,401,328 (GRCm39)	Q95L	probably benign	Het
Rnf169	T	A	7: 99,574,732 (GRCm39)	N621I	probably damaging	Het
Rps27a	T	C	11: 29,497,229 (GRCm39)	K27R	probably null	Het
Sar1a	T	C	10: 61,520,947 (GRCm39)	V54A	probably benign	Het
Sash1	T	A	10: 8,605,477 (GRCm39)	H971L	probably benign	Het
Sdk2	G	A	11: 113,671,843 (GRCm39)	Q2102*	probably null	Het
Serpina1e	T	A	12: 103,917,466 (GRCm39)	I68F	probably damaging	Het
Serpinb9e	A	C	13: 33,437,474 (GRCm39)	Q119P	probably benign	Het
Sh3tc2	A	T	18: 62,124,226 (GRCm39)	K965*	probably null	Het
Slc16a1	A	T	3: 104,556,782 (GRCm39)	S56C	probably damaging	Het
Slc26a11	T	A	11: 119,271,020 (GRCm39)	L563Q	possibly damaging	Het
Smarca2	G	T	19: 26,650,124 (GRCm39)	E24*	probably null	Het
Smgc	G	A	15: 91,744,468 (GRCm39)	G239D	probably damaging	Het
Sos2	A	T	12: 69,663,636 (GRCm39)	M616K	possibly damaging	Het
Tet1	T	A	10: 62,648,726 (GRCm39)	D1902V	possibly damaging	Het
Thap2	C	T	10: 115,220,152 (GRCm39)	C10Y	probably damaging	Het
Tln2	T	C	9: 67,249,417 (GRCm39)	D890G	probably benign	Het
Tor2a	G	A	2: 32,648,716 (GRCm39)	G62D	probably damaging	Het
Ubqln5	A	T	7: 103,778,095 (GRCm39)	V243E	possibly damaging	Het
Utrn	A	T	10: 12,560,181 (GRCm39)	D1369E	probably damaging	Het
Vcan	A	G	13: 89,840,861 (GRCm39)	V1561A	probably benign	Het
Vmn1r70	T	C	7: 10,367,737 (GRCm39)	F56S	possibly damaging	Het
Vmn2r25	A	G	6: 123,800,254 (GRCm39)	L696S	possibly damaging	Het
Washc2	A	G	6: 116,185,948 (GRCm39)	T53A	probably damaging	Het
Wnk1	T	A	6: 119,911,343 (GRCm39)	T2417S	possibly damaging	Het
Zbtb49	A	T	5: 38,361,105 (GRCm39)	C12*	probably null	Het
Zfp472	C	A	17: 33,196,848 (GRCm39)	P308T	possibly damaging	Het

Other mutations in Dcp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02801:Dcp2	APN	18	44,550,778 (GRCm39)	missense	probably damaging	1.00
belay	UTSW	18	44,529,019 (GRCm39)	missense	probably damaging	0.99
PIT4431001:Dcp2	UTSW	18	44,545,638 (GRCm39)	missense	probably benign	0.15
R0051:Dcp2	UTSW	18	44,538,441 (GRCm39)	splice site	probably benign
R0515:Dcp2	UTSW	18	44,532,798 (GRCm39)	missense	probably benign	0.41
R0761:Dcp2	UTSW	18	44,543,300 (GRCm39)	missense	probably benign	0.01
R1696:Dcp2	UTSW	18	44,533,391 (GRCm39)	missense	probably damaging	1.00
R1803:Dcp2	UTSW	18	44,528,984 (GRCm39)	missense	probably damaging	1.00
R1928:Dcp2	UTSW	18	44,538,638 (GRCm39)	critical splice donor site	probably null
R2014:Dcp2	UTSW	18	44,543,363 (GRCm39)	missense	probably benign	0.00
R2209:Dcp2	UTSW	18	44,538,581 (GRCm39)	nonsense	probably null
R4167:Dcp2	UTSW	18	44,529,034 (GRCm39)	missense	probably damaging	1.00
R4668:Dcp2	UTSW	18	44,548,429 (GRCm39)	splice site	probably null
R4877:Dcp2	UTSW	18	44,550,659 (GRCm39)	missense	probably benign	0.11
R5147:Dcp2	UTSW	18	44,550,662 (GRCm39)	nonsense	probably null
R5559:Dcp2	UTSW	18	44,538,554 (GRCm39)	missense	probably damaging	1.00
R6533:Dcp2	UTSW	18	44,532,731 (GRCm39)	missense	probably benign	0.25
R7406:Dcp2	UTSW	18	44,543,254 (GRCm39)	missense	probably benign	0.00
R7469:Dcp2	UTSW	18	44,529,019 (GRCm39)	missense	probably damaging	0.99
R7850:Dcp2	UTSW	18	44,533,415 (GRCm39)	nonsense	probably null
R8054:Dcp2	UTSW	18	44,538,774 (GRCm39)	missense	probably benign	0.02
R8315:Dcp2	UTSW	18	44,529,071 (GRCm39)	missense	probably benign	0.01
R9422:Dcp2	UTSW	18	44,538,361 (GRCm39)	missense	probably damaging	1.00
R9423:Dcp2	UTSW	18	44,538,361 (GRCm39)	missense	probably damaging	1.00
R9424:Dcp2	UTSW	18	44,538,361 (GRCm39)	missense	probably damaging	1.00
R9425:Dcp2	UTSW	18	44,538,361 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCGCCTTTCCTAGTTGGTGG -3'
(R):5'- CACTTCAGTGCCGCGAAAA -3'

Sequencing Primer
(F):5'- TGGGTGGGGTAAGGAAGTAAATATG -3'
(R):5'- AGCGAAACTGCCTCACGTG -3'

Posted On

2014-08-01