Mutagenetix > Incidental Mutation

Incidental Mutation 'R1969:Fryl'

219428

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

9030227G01Rik, 2310004H21Rik, 2510002A14Rik

MMRRC Submission

039982-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.740) question?

Stock #

R1969 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

73177534-73414042 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 73255609 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 807 (S807R)

Ref Sequence

ENSEMBL: ENSMUSP00000098687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094700
AA Change: S807R

PolyPhen 2 Score 0.178 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: S807R

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000101127
AA Change: S807R

PolyPhen 2 Score 0.178 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: S807R

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153923

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000201405

SMART Domains

Protein: ENSMUSP00000144346
Gene: ENSMUSG00000070733

Domain	Start	End	E-Value	Type
low complexity region	103	114	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.6%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 105 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059E24Rik	T	C	19: 21,575,609 (GRCm39)		probably benign	Het
Apoh	G	A	11: 108,298,288 (GRCm39)	R196Q	probably benign	Het
Arnt	C	T	3: 95,355,704 (GRCm39)	S16L	possibly damaging	Het
B4galnt4	T	C	7: 140,644,761 (GRCm39)	F194L	probably benign	Het
BC034090	A	T	1: 155,100,972 (GRCm39)	L431M	possibly damaging	Het
Bdh2	G	T	3: 134,994,040 (GRCm39)	A31S	probably damaging	Het
Brd10	C	T	19: 29,694,075 (GRCm39)	S1873N	possibly damaging	Het
Cacna1s	T	C	1: 136,046,833 (GRCm39)	S1821P	probably benign	Het
Caskin1	A	T	17: 24,725,824 (GRCm39)	Q1370L	possibly damaging	Het
Ccdc83	T	A	7: 89,893,362 (GRCm39)	S132C	probably damaging	Het
Cdk15	A	T	1: 59,370,110 (GRCm39)	H384L	probably damaging	Het
Col24a1	T	C	3: 145,020,691 (GRCm39)	I354T	probably benign	Het
Coro7	T	C	16: 4,451,620 (GRCm39)	I451V	probably benign	Het
Ctnna2	T	C	6: 77,735,483 (GRCm39)	E65G	probably damaging	Het
Ctsll3	A	T	13: 60,948,162 (GRCm39)	W172R	probably benign	Het
D630045J12Rik	T	C	6: 38,145,078 (GRCm39)	D1316G	probably damaging	Het
Ddx19b	C	T	8: 111,734,890 (GRCm39)	A493T	probably benign	Het
Ddx4	C	T	13: 112,736,547 (GRCm39)	V608I	probably damaging	Het
Ddx4	T	A	13: 112,757,276 (GRCm39)	H348L	probably damaging	Het
Dnah17	G	C	11: 117,995,361 (GRCm39)	Q996E	probably benign	Het
Dnah9	G	C	11: 65,739,197 (GRCm39)	N4180K	probably damaging	Het
Dok7	A	G	5: 35,234,610 (GRCm39)		probably null	Het
Dpf3	T	A	12: 83,371,809 (GRCm39)		probably null	Het
Efcab3	A	G	11: 104,637,090 (GRCm39)	I988M	probably damaging	Het
Elfn1	G	C	5: 139,958,604 (GRCm39)	R536P	probably damaging	Het
Eml6	A	G	11: 29,783,075 (GRCm39)	V602A	probably benign	Het
Enpp2	T	C	15: 54,746,378 (GRCm39)	D296G	probably damaging	Het
Evi5	A	T	5: 107,896,230 (GRCm39)	F738I	probably benign	Het
Eya2	T	C	2: 165,558,039 (GRCm39)	S212P	probably benign	Het
Fam83a	T	C	15: 57,849,498 (GRCm39)	L14P	probably damaging	Het
Fanca	A	T	8: 124,014,803 (GRCm39)	M735K	probably benign	Het
Fancm	T	A	12: 65,148,466 (GRCm39)	S694T	probably benign	Het
Fibcd1	G	A	2: 31,706,673 (GRCm39)	T386I	probably damaging	Het
Foxd4	A	G	19: 24,877,178 (GRCm39)	S341P	probably benign	Het
Foxp4	C	T	17: 48,186,796 (GRCm39)	R378Q	unknown	Het
Fscb	T	C	12: 64,520,008 (GRCm39)	E486G	unknown	Het
Galnt17	A	G	5: 131,179,782 (GRCm39)	S122P	probably benign	Het
Ghitm	A	G	14: 36,853,586 (GRCm39)	F85L	probably benign	Het
Ghrh	C	A	2: 157,175,386 (GRCm39)	V32L	probably benign	Het
Gli2	C	T	1: 118,765,430 (GRCm39)	R907H	probably benign	Het
Gm28042	A	G	2: 119,872,096 (GRCm39)	*1015W	probably null	Het
Gpr179	T	C	11: 97,228,784 (GRCm39)	T1124A	probably benign	Het
Grid2	C	A	6: 63,885,902 (GRCm39)	C99*	probably null	Het
Grin1	A	T	2: 25,187,927 (GRCm39)	M523K	probably benign	Het
Gucy2g	T	C	19: 55,211,328 (GRCm39)	Y634C	possibly damaging	Het
Gucy2g	T	G	19: 55,221,485 (GRCm39)	T339P	probably benign	Het
Haus6	A	T	4: 86,522,483 (GRCm39)	L116H	probably damaging	Het
Hey1	T	C	3: 8,731,879 (GRCm39)	T18A	probably benign	Het
Hipk3	T	C	2: 104,264,186 (GRCm39)	N792D	probably damaging	Het
Il19	A	T	1: 130,866,893 (GRCm39)	L29Q	probably damaging	Het
Il21r	A	G	7: 125,228,144 (GRCm39)	Q205R	probably damaging	Het
Kcnip2	T	C	19: 45,782,122 (GRCm39)	D169G	probably null	Het
Kctd18	T	C	1: 58,006,779 (GRCm39)	I24V	probably benign	Het
Lcp1	T	C	14: 75,437,946 (GRCm39)	S119P	probably damaging	Het
Lig3	G	C	11: 82,686,544 (GRCm39)	D642H	probably benign	Het
Lrba	A	G	3: 86,515,696 (GRCm39)	K2166E	probably damaging	Het
Lrrn2	T	C	1: 132,866,972 (GRCm39)	V679A	probably benign	Het
Lyst	G	A	13: 13,904,929 (GRCm39)	R3202H	probably damaging	Het
Micall2	A	G	5: 139,721,885 (GRCm39)	C11R	probably damaging	Het
Morc2b	T	G	17: 33,356,065 (GRCm39)	Q569P	probably benign	Het
Mtrf1	A	G	14: 79,639,111 (GRCm39)	E81G	probably damaging	Het
Myh2	G	T	11: 67,080,004 (GRCm39)	S1099I	possibly damaging	Het
Nap1l1	T	A	10: 111,326,914 (GRCm39)	D158E	probably benign	Het
Nckap5l	T	A	15: 99,320,699 (GRCm39)	T1285S	probably damaging	Het
Nsrp1	A	T	11: 76,936,612 (GRCm39)	M528K	probably damaging	Het
Numa1	C	T	7: 101,658,529 (GRCm39)	A1605V	probably damaging	Het
Nutm2	T	C	13: 50,627,878 (GRCm39)	L453P	probably damaging	Het
Ofd1	T	C	X: 165,210,210 (GRCm39)	Y205C	probably benign	Het
Or2ag12	T	A	7: 106,276,877 (GRCm39)	N272I	probably damaging	Het
Or2n1c	A	G	17: 38,519,355 (GRCm39)	Y73C	probably damaging	Het
Or4c125	T	A	2: 89,170,640 (GRCm39)	N2I	probably damaging	Het
Or52k2	A	T	7: 102,253,777 (GRCm39)	D72V	probably damaging	Het
Or7a40	T	A	16: 16,491,447 (GRCm39)	M133L	probably benign	Het
Patl1	T	C	19: 11,898,782 (GRCm39)	L159P	probably benign	Het
Paxip1	G	A	5: 27,949,134 (GRCm39)	T1045I	probably damaging	Het
Phaf1	T	A	8: 105,973,132 (GRCm39)	D247E	possibly damaging	Het
Pik3ca	A	G	3: 32,505,903 (GRCm39)		probably null	Het
Pkhd1	T	A	1: 20,451,747 (GRCm39)	I2183F	probably damaging	Het
Plec	C	T	15: 76,073,372 (GRCm39)	R319H	probably damaging	Het
Pnkd	G	A	1: 74,391,008 (GRCm39)	G334D	probably damaging	Het
Prss50	A	G	9: 110,691,449 (GRCm39)	Y251C	probably damaging	Het
Rab11b	A	T	17: 33,979,209 (GRCm39)	Y10N	probably damaging	Het
Rfc1	G	A	5: 65,476,867 (GRCm39)	R4W	probably damaging	Het
Rgs19	A	T	2: 181,331,276 (GRCm39)	F119Y	probably damaging	Het
Safb	A	G	17: 56,912,821 (GRCm39)	H883R	probably benign	Het
Serpine1	G	A	5: 137,096,601 (GRCm39)	Q227*	probably null	Het
Slc25a13	A	G	6: 6,096,668 (GRCm39)		probably null	Het
Slc6a18	T	A	13: 73,812,308 (GRCm39)	T502S	possibly damaging	Het
Sox4	T	C	13: 29,136,631 (GRCm39)	D125G	probably damaging	Het
Spata31d1e	T	C	13: 59,890,599 (GRCm39)	D407G	probably damaging	Het
Stag3	C	T	5: 138,298,400 (GRCm39)	T731I	probably damaging	Het
Thada	G	T	17: 84,617,470 (GRCm39)	P1349T	probably damaging	Het
Tmem245	C	A	4: 56,937,964 (GRCm39)	V195F	probably benign	Het
Tnxb	A	G	17: 34,898,055 (GRCm39)	H901R	probably benign	Het
Trim39	A	T	17: 36,579,645 (GRCm39)	D103E	probably benign	Het
Ttn	A	T	2: 76,562,304 (GRCm39)	V28847E	probably damaging	Het
Tubb1	A	T	2: 174,297,484 (GRCm39)	D31V	possibly damaging	Het
Ush1g	A	G	11: 115,209,280 (GRCm39)	S305P	probably damaging	Het
Vmn1r191	A	T	13: 22,362,952 (GRCm39)	N267K	possibly damaging	Het
Vmn1r59	A	T	7: 5,457,038 (GRCm39)	Y241N	probably damaging	Het
Vmn2r49	T	C	7: 9,720,235 (GRCm39)	N419D	probably damaging	Het
Vps35	T	C	8: 86,005,623 (GRCm39)	D326G	possibly damaging	Het
Xdh	A	G	17: 74,199,746 (GRCm39)	S1187P	possibly damaging	Het
Xpc	A	C	6: 91,478,007 (GRCm39)		probably null	Het
Zfp273	T	C	13: 67,973,282 (GRCm39)	Y104H	probably damaging	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,305,451 (GRCm39)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,244,305 (GRCm39)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,211,940 (GRCm39)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,179,844 (GRCm39)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,279,707 (GRCm39)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,190,134 (GRCm39)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,282,112 (GRCm39)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,233,302 (GRCm39)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,267,519 (GRCm39)	splice site	probably benign
IGL02431:Fryl	APN	5	73,255,651 (GRCm39)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,222,636 (GRCm39)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,255,736 (GRCm39)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,227,220 (GRCm39)	intron	probably benign
IGL02642:Fryl	APN	5	73,252,809 (GRCm39)	missense	probably benign
IGL02657:Fryl	APN	5	73,212,203 (GRCm39)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,250,506 (GRCm39)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,216,726 (GRCm39)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,258,798 (GRCm39)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03183:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03275:Fryl	APN	5	73,305,376 (GRCm39)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,293,659 (GRCm39)	splice site	probably benign
IGL03341:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03343:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03350:Fryl	APN	5	73,290,649 (GRCm39)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,211,402 (GRCm39)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,267,624 (GRCm39)	splice site	probably benign
IGL03375:Fryl	APN	5	73,245,792 (GRCm39)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
Besotted	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,198,947 (GRCm39)	splice site	probably benign
R0312:Fryl	UTSW	5	73,230,231 (GRCm39)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,255,757 (GRCm39)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,244,315 (GRCm39)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,254,760 (GRCm39)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,221,840 (GRCm39)	splice site	probably benign
R0567:Fryl	UTSW	5	73,222,734 (GRCm39)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,282,077 (GRCm39)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,226,074 (GRCm39)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,222,702 (GRCm39)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,228,469 (GRCm39)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,246,539 (GRCm39)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,198,675 (GRCm39)	splice site	probably benign
R1076:Fryl	UTSW	5	73,282,016 (GRCm39)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,267,614 (GRCm39)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,222,268 (GRCm39)	splice site	probably benign
R1394:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,232,094 (GRCm39)	nonsense	probably null
R1664:Fryl	UTSW	5	73,216,778 (GRCm39)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,190,204 (GRCm39)	splice site	probably benign
R1937:Fryl	UTSW	5	73,290,710 (GRCm39)	missense	probably damaging	1.00
R1993:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,179,465 (GRCm39)	nonsense	probably null
R2036:Fryl	UTSW	5	73,265,305 (GRCm39)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,179,887 (GRCm39)	missense	probably benign
R2088:Fryl	UTSW	5	73,222,804 (GRCm39)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,279,642 (GRCm39)	missense	probably benign
R2106:Fryl	UTSW	5	73,255,674 (GRCm39)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,222,318 (GRCm39)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,265,890 (GRCm39)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,207,799 (GRCm39)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,240,193 (GRCm39)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,265,417 (GRCm39)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3787:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3837:Fryl	UTSW	5	73,228,608 (GRCm39)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,269,766 (GRCm39)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,243,903 (GRCm39)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,245,740 (GRCm39)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,238,396 (GRCm39)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,248,022 (GRCm39)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,257,636 (GRCm39)	missense	probably benign
R4700:Fryl	UTSW	5	73,222,881 (GRCm39)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,238,315 (GRCm39)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,198,705 (GRCm39)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,226,125 (GRCm39)	frame shift	probably null
R4948:Fryl	UTSW	5	73,246,473 (GRCm39)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,192,401 (GRCm39)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,233,236 (GRCm39)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,215,098 (GRCm39)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,248,061 (GRCm39)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,259,016 (GRCm39)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,243,943 (GRCm39)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,222,479 (GRCm39)	nonsense	probably null
R5275:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,262,117 (GRCm39)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,231,247 (GRCm39)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,257,808 (GRCm39)	missense	probably benign
R5778:Fryl	UTSW	5	73,230,121 (GRCm39)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,248,098 (GRCm39)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,248,060 (GRCm39)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,254,715 (GRCm39)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,240,638 (GRCm39)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,257,340 (GRCm39)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,275,894 (GRCm39)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,270,131 (GRCm39)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,222,824 (GRCm39)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,349,102 (GRCm39)	intron	probably benign
R6310:Fryl	UTSW	5	73,349,104 (GRCm39)	intron	probably benign
R6429:Fryl	UTSW	5	73,248,094 (GRCm39)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,216,859 (GRCm39)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,290,655 (GRCm39)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,289,824 (GRCm39)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,212,124 (GRCm39)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,179,575 (GRCm39)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,222,437 (GRCm39)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,222,560 (GRCm39)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,222,375 (GRCm39)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,226,146 (GRCm39)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,179,485 (GRCm39)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,179,554 (GRCm39)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,231,272 (GRCm39)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,207,773 (GRCm39)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,212,951 (GRCm39)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,248,099 (GRCm39)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,231,251 (GRCm39)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,279,653 (GRCm39)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,222,438 (GRCm39)	missense	probably benign
R7236:Fryl	UTSW	5	73,265,821 (GRCm39)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,204,839 (GRCm39)	splice site	probably null
R7425:Fryl	UTSW	5	73,262,091 (GRCm39)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,181,331 (GRCm39)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,254,904 (GRCm39)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,255,539 (GRCm39)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,180,019 (GRCm39)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,238,382 (GRCm39)	missense	probably benign
R7548:Fryl	UTSW	5	73,349,105 (GRCm39)	missense	unknown
R7565:Fryl	UTSW	5	73,191,063 (GRCm39)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,245,739 (GRCm39)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,179,843 (GRCm39)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,267,588 (GRCm39)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,240,727 (GRCm39)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,228,641 (GRCm39)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,211,875 (GRCm39)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,259,150 (GRCm39)	splice site	probably null
R8110:Fryl	UTSW	5	73,290,620 (GRCm39)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,228,527 (GRCm39)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,207,682 (GRCm39)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,257,843 (GRCm39)	splice site	probably null
R8263:Fryl	UTSW	5	73,238,348 (GRCm39)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8359:Fryl	UTSW	5	73,233,276 (GRCm39)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,293,663 (GRCm39)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,275,790 (GRCm39)	makesense	probably null
R8450:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8514:Fryl	UTSW	5	73,242,699 (GRCm39)	missense	probably benign
R8536:Fryl	UTSW	5	73,257,696 (GRCm39)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,247,997 (GRCm39)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,289,905 (GRCm39)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,226,185 (GRCm39)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,255,609 (GRCm39)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,182,118 (GRCm39)	missense
R9063:Fryl	UTSW	5	73,238,346 (GRCm39)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,265,920 (GRCm39)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,348,862 (GRCm39)	intron	probably benign
R9345:Fryl	UTSW	5	73,207,754 (GRCm39)	missense	probably benign
R9381:Fryl	UTSW	5	73,240,637 (GRCm39)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,349,152 (GRCm39)	missense	unknown
R9401:Fryl	UTSW	5	73,222,563 (GRCm39)	nonsense	probably null
R9497:Fryl	UTSW	5	73,215,134 (GRCm39)	missense
R9514:Fryl	UTSW	5	73,262,115 (GRCm39)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,179,498 (GRCm39)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,275,801 (GRCm39)	missense	probably benign
R9665:Fryl	UTSW	5	73,222,299 (GRCm39)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,216,879 (GRCm39)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,192,402 (GRCm39)	missense	probably benign
Z1088:Fryl	UTSW	5	73,248,081 (GRCm39)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,248,052 (GRCm39)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,230,180 (GRCm39)	missense	probably benign
Z1177:Fryl	UTSW	5	73,198,938 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- GTGCCACTTTTCAAAAGCACAC -3'
(R):5'- TTGAAAGTTACTCAGATGCGTG -3'

Sequencing Primer
(F):5'- CATCATGAAAGCTGACTACCCATTG -3'
(R):5'- CTCAGATGCGTGATAATCATTTGTGC -3'

Posted On

2014-08-25