Mutagenetix > Incidental Mutation

Incidental Mutation 'R1970:Col27a1'

219666

Institutional Source

Beutler Lab

Gene Symbol

Col27a1

Ensembl Gene

ENSMUSG00000045672

Gene Name

collagen, type XXVII, alpha 1

Synonyms

5730512J02Rik

MMRRC Submission

039983-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1970 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

63132246-63253228 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

C to T at 63191354 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000139173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036300] [ENSMUST00000184067]

AlphaFold

Q5QNQ9

Predicted Effect

probably benign
Transcript: ENSMUST00000036300

SMART Domains

Protein: ENSMUSP00000043816
Gene: ENSMUSG00000045672

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
TSPN	43	223	1.1e-5	SMART
low complexity region	325	343	N/A	INTRINSIC
low complexity region	356	372	N/A	INTRINSIC
low complexity region	428	443	N/A	INTRINSIC
low complexity region	455	467	N/A	INTRINSIC
low complexity region	584	597	N/A	INTRINSIC
Pfam:Collagen	609	670	2.1e-10	PFAM
Pfam:Collagen	666	731	3.7e-10	PFAM
low complexity region	790	808	N/A	INTRINSIC
low complexity region	817	838	N/A	INTRINSIC
low complexity region	858	880	N/A	INTRINSIC
low complexity region	886	910	N/A	INTRINSIC
low complexity region	912	946	N/A	INTRINSIC
Pfam:Collagen	1012	1080	2.8e-8	PFAM
Pfam:Collagen	1033	1103	3e-9	PFAM
Pfam:Collagen	1063	1130	3.4e-9	PFAM
low complexity region	1150	1168	N/A	INTRINSIC
Pfam:Collagen	1207	1281	5.5e-9	PFAM
Pfam:Collagen	1261	1324	8.4e-10	PFAM
Pfam:Collagen	1323	1384	3.8e-12	PFAM
low complexity region	1438	1466	N/A	INTRINSIC
internal_repeat_4	1467	1502	1.5e-7	PROSPERO
internal_repeat_2	1468	1529	1.96e-8	PROSPERO
Pfam:Collagen	1544	1606	2.4e-9	PFAM
COLFI	1644	1845	1.28e-40	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125504

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148751

Predicted Effect

probably benign
Transcript: ENSMUST00000184067

SMART Domains

Protein: ENSMUSP00000139173
Gene: ENSMUSG00000045672

Domain	Start	End	E-Value	Type
Pfam:Collagen	23	87	2.1e-8	PFAM
Pfam:Collagen	57	145	8.3e-8	PFAM
Pfam:Collagen	115	200	9.9e-8	PFAM
low complexity region	202	223	N/A	INTRINSIC
low complexity region	243	265	N/A	INTRINSIC
low complexity region	271	295	N/A	INTRINSIC
low complexity region	297	331	N/A	INTRINSIC
internal_repeat_1	337	442	5.17e-20	PROSPERO
Pfam:Collagen	448	515	1.5e-9	PFAM
Pfam:Collagen	478	543	2e-10	PFAM
Pfam:Collagen	502	566	2.5e-9	PFAM
Pfam:Collagen	532	617	4.4e-7	PFAM
Pfam:Collagen	594	660	8.2e-11	PFAM
Pfam:Collagen	649	709	1.4e-10	PFAM
Pfam:Collagen	708	769	2e-12	PFAM
Pfam:Collagen	752	829	5e-8	PFAM
Pfam:Collagen	878	939	2.2e-9	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 94.2%

Validation Efficiency

97% (121/125)

MGI Phenotype

FUNCTION: This gene encodes the alpha-1 subunit of type XXVII collagen, one of the low abundance fibril-forming collagens found in cartilage. The encoded protein forms a homotrimeric triple helical procollagen that undergoes proteolytic processing during fibril formation. Transgenic mice lacking a portion of the collagenous domain in the encoded protein exhibit skeletal abnormalities, chondrodysplasia and die at birth because of a lung defect. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for an in frame deletion display neonatal lethality, respiratory failure, and severe chondrodysplasia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 121 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	T	17: 24,526,549 (GRCm39)	H578Q	probably benign	Het
Abcc12	A	G	8: 87,253,910 (GRCm39)	I958T	probably benign	Het
Abcg5	AATCATTTG	AG	17: 84,981,030 (GRCm39)		probably null	Het
Acap2	A	G	16: 30,952,345 (GRCm39)		probably null	Het
Adgrb1	T	C	15: 74,411,726 (GRCm39)		probably benign	Het
Akap6	T	A	12: 52,985,258 (GRCm39)	V897E	probably damaging	Het
Als2	A	T	1: 59,254,328 (GRCm39)	L343Q	probably benign	Het
Arhgap5	A	G	12: 52,589,376 (GRCm39)	I1275M	probably damaging	Het
Arnt	C	T	3: 95,355,704 (GRCm39)	S16L	possibly damaging	Het
Bglap3	C	T	3: 88,284,300 (GRCm39)		probably benign	Het
Blnk	T	C	19: 40,928,609 (GRCm39)		probably benign	Het
C1qtnf4	T	C	2: 90,720,003 (GRCm39)	M92T	probably damaging	Het
Ccdc177	G	A	12: 80,805,486 (GRCm39)	R263C	unknown	Het
Ccdc83	T	A	7: 89,893,362 (GRCm39)	S132C	probably damaging	Het
Cdc7	A	G	5: 107,120,940 (GRCm39)		probably benign	Het
Cgnl1	T	A	9: 71,632,817 (GRCm39)	N178I	probably benign	Het
Col5a1	A	T	2: 27,876,766 (GRCm39)	M822L	unknown	Het
Coro7	T	C	16: 4,451,620 (GRCm39)	I451V	probably benign	Het
Csmd3	T	C	15: 48,536,927 (GRCm39)	T92A	probably damaging	Het
Ddc	A	G	11: 11,765,292 (GRCm39)	V460A	possibly damaging	Het
Ddx4	C	T	13: 112,736,547 (GRCm39)	V608I	probably damaging	Het
Ddx60	A	T	8: 62,425,240 (GRCm39)	H676L	possibly damaging	Het
Dmtf1	T	C	5: 9,198,989 (GRCm39)	E48G	probably benign	Het
Dpf3	T	A	12: 83,371,809 (GRCm39)		probably null	Het
Dydc2	A	G	14: 40,783,860 (GRCm39)	C88R	probably benign	Het
Elovl4	A	G	9: 83,662,772 (GRCm39)	Y163H	probably damaging	Het
Enpp2	T	C	15: 54,746,378 (GRCm39)	D296G	probably damaging	Het
Fam83h	G	T	15: 75,878,419 (GRCm39)		probably benign	Het
Fbf1	T	A	11: 116,042,317 (GRCm39)	Q511L	possibly damaging	Het
Fhdc1	A	T	3: 84,362,158 (GRCm39)	L323Q	probably damaging	Het
Fmnl2	T	A	2: 52,995,588 (GRCm39)	V437D	possibly damaging	Het
Foxo3	A	G	10: 42,073,258 (GRCm39)	S420P	probably benign	Het
Foxp4	C	T	17: 48,186,796 (GRCm39)	R378Q	unknown	Het
Garem2	G	T	5: 30,322,172 (GRCm39)	G844*	probably null	Het
Glmp	T	C	3: 88,235,177 (GRCm39)	L269S	probably damaging	Het
Gm9915	T	C	1: 42,269,881 (GRCm39)		noncoding transcript	Het
Gnb4	A	T	3: 32,652,290 (GRCm39)	D27E	probably damaging	Het
Gnb5	A	G	9: 75,251,932 (GRCm39)		probably null	Het
Gpr161	T	G	1: 165,133,927 (GRCm39)	V63G	probably damaging	Het
Gsk3a	T	A	7: 24,929,146 (GRCm39)		probably benign	Het
Hapln2	T	C	3: 87,931,427 (GRCm39)		probably null	Het
Incenp	G	T	19: 9,862,851 (GRCm39)	T401N	unknown	Het
Kalrn	A	G	16: 33,797,894 (GRCm39)		probably null	Het
Kcnq3	A	G	15: 65,900,472 (GRCm39)		probably null	Het
Kif21b	T	C	1: 136,098,894 (GRCm39)	V1394A	probably damaging	Het
Klhl23	T	C	2: 69,664,030 (GRCm39)	C460R	probably damaging	Het
L3mbtl1	G	A	2: 162,801,492 (GRCm39)	A291T	probably damaging	Het
Lcp1	T	C	14: 75,437,946 (GRCm39)	S119P	probably damaging	Het
Ldhc	A	G	7: 46,519,175 (GRCm39)	I133V	probably benign	Het
Lmntd2	G	A	7: 140,791,972 (GRCm39)		probably benign	Het
Lpl	A	T	8: 69,349,454 (GRCm39)	K327*	probably null	Het
Lrp1b	T	C	2: 40,765,081 (GRCm39)	D2801G	probably damaging	Het
Mppe1	C	A	18: 67,362,843 (GRCm39)	A131S	probably benign	Het
Msh3	T	A	13: 92,386,328 (GRCm39)		probably benign	Het
Msh5	A	G	17: 35,252,576 (GRCm39)	I377T	probably damaging	Het
Myo1e	T	C	9: 70,276,055 (GRCm39)	F757L	probably benign	Het
Myof	A	T	19: 37,934,082 (GRCm39)	D955E	probably damaging	Het
Nckap1	C	T	2: 80,348,286 (GRCm39)	S889N	probably benign	Het
Ncor2	T	C	5: 125,115,982 (GRCm39)	D858G	probably damaging	Het
Neb	A	T	2: 52,153,917 (GRCm39)	V2398D	possibly damaging	Het
Nefl	C	G	14: 68,324,121 (GRCm39)	T453R	probably benign	Het
Nf1	A	G	11: 79,444,787 (GRCm39)	N371D	probably benign	Het
Nhsl3	A	G	4: 129,119,810 (GRCm39)		probably benign	Het
Nlrp4f	T	C	13: 65,341,905 (GRCm39)	Y580C	probably damaging	Het
Nme8	A	C	13: 19,836,492 (GRCm39)	L228R	probably damaging	Het
Numa1	C	T	7: 101,658,529 (GRCm39)	A1605V	probably damaging	Het
Ofd1	T	C	X: 165,210,210 (GRCm39)	Y205C	probably benign	Het
Or2ag12	T	A	7: 106,276,877 (GRCm39)	N272I	probably damaging	Het
Or2d3b	G	A	7: 106,513,453 (GRCm39)	G16D	probably damaging	Het
Or4k77	C	T	2: 111,199,421 (GRCm39)	S148L	probably benign	Het
Or8g55	T	A	9: 39,785,234 (GRCm39)	I221N	probably damaging	Het
Or8k3	T	A	2: 86,058,596 (GRCm39)	T240S	probably damaging	Het
Pclo	A	G	5: 14,763,487 (GRCm39)	T3987A	unknown	Het
Pdgfrb	G	A	18: 61,199,566 (GRCm39)		probably benign	Het
Pdxk	G	A	10: 78,276,988 (GRCm39)	T270I	probably damaging	Het
Pex5	T	C	6: 124,391,364 (GRCm39)	E10G	probably damaging	Het
Pik3c2g	T	A	6: 139,846,112 (GRCm39)		probably null	Het
Pkhd1	T	A	1: 20,451,747 (GRCm39)	I2183F	probably damaging	Het
Plppr2	T	C	9: 21,852,422 (GRCm39)	V102A	probably damaging	Het
Plxnd1	T	C	6: 115,939,478 (GRCm39)	T1449A	probably damaging	Het
Pnkd	T	A	1: 74,325,069 (GRCm39)		probably null	Het
Pom121l2	A	G	13: 22,167,642 (GRCm39)	I638V	probably damaging	Het
Prag1	A	G	8: 36,596,314 (GRCm39)		probably null	Het
Ranbp10	A	G	8: 106,513,340 (GRCm39)	F191L	probably damaging	Het
Rapgef1	T	C	2: 29,623,723 (GRCm39)	L824P	probably damaging	Het
Rec8	T	C	14: 55,861,599 (GRCm39)	L418P	probably damaging	Het
Rimbp2	T	A	5: 128,874,305 (GRCm39)	N429Y	probably damaging	Het
Rpe65	C	A	3: 159,321,307 (GRCm39)	T373K	probably benign	Het
Rpl35rt	T	G	1: 156,194,017 (GRCm39)	*124G	probably null	Het
Rrn3	T	C	16: 13,606,938 (GRCm39)	S151P	probably damaging	Het
Scn7a	A	G	2: 66,514,633 (GRCm39)	V1047A	possibly damaging	Het
Scn9a	G	T	2: 66,345,724 (GRCm39)	P1123Q	probably damaging	Het
Secisbp2l	T	C	2: 125,589,430 (GRCm39)	D706G	probably damaging	Het
Serpina5	A	T	12: 104,070,116 (GRCm39)	T338S	probably benign	Het
Sez6	G	A	11: 77,844,894 (GRCm39)		probably null	Het
Shisa4	C	T	1: 135,300,012 (GRCm39)	G157D	probably damaging	Het
Slc1a7	G	T	4: 107,825,782 (GRCm39)	D14Y	probably benign	Het
Slc25a11	A	C	11: 70,536,999 (GRCm39)	L51V	probably benign	Het
Slit3	A	G	11: 35,521,668 (GRCm39)		probably null	Het
Spata13	G	T	14: 60,928,912 (GRCm39)	G157W	probably damaging	Het
Spta1	T	C	1: 174,067,933 (GRCm39)	V2120A	possibly damaging	Het
Srpra	T	C	9: 35,124,834 (GRCm39)		probably null	Het
Syt6	A	G	3: 103,494,736 (GRCm39)	I234V	probably benign	Het
Thada	G	T	17: 84,617,470 (GRCm39)	P1349T	probably damaging	Het
Tmem161a	C	T	8: 70,629,559 (GRCm39)	R58W	probably damaging	Het
Top3b	A	G	16: 16,701,383 (GRCm39)	I232V	probably damaging	Het
Tspan1	T	A	4: 116,020,826 (GRCm39)	Q197L	possibly damaging	Het
Ttc28	A	C	5: 111,383,501 (GRCm39)	Y1334S	probably benign	Het
Ubxn6	G	T	17: 56,380,077 (GRCm39)	N28K	possibly damaging	Het
Uggt1	T	C	1: 36,190,862 (GRCm39)	D1366G	probably damaging	Het
Ugp2	A	G	11: 21,278,942 (GRCm39)	S415P	probably damaging	Het
Vcan	A	T	13: 89,837,157 (GRCm39)	S2796T	probably damaging	Het
Vipr2	T	C	12: 116,099,826 (GRCm39)	V231A	probably benign	Het
Vmn1r176	T	C	7: 23,534,373 (GRCm39)	N260S	probably benign	Het
Vmn1r59	A	T	7: 5,457,038 (GRCm39)	Y241N	probably damaging	Het
Vmn2r67	A	T	7: 84,801,013 (GRCm39)	Y308N	probably benign	Het
Vmn2r75	A	T	7: 85,797,470 (GRCm39)	M781K	probably damaging	Het
Vwa3a	A	T	7: 120,379,394 (GRCm39)	I500F	probably damaging	Het
Zfp609	A	G	9: 65,702,559 (GRCm39)	V31A	probably damaging	Het
Zfp689	G	T	7: 127,043,959 (GRCm39)	Q224K	probably damaging	Het
Zfp81	A	T	17: 33,554,475 (GRCm39)	L113H	probably benign	Het

Other mutations in Col27a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01305:Col27a1	APN	4	63,218,978 (GRCm39)	splice site	probably benign
IGL01461:Col27a1	APN	4	63,142,480 (GRCm39)	missense	probably damaging	1.00
IGL01534:Col27a1	APN	4	63,144,019 (GRCm39)	missense	probably benign	0.12
IGL01738:Col27a1	APN	4	63,182,016 (GRCm39)	splice site	probably benign
IGL01810:Col27a1	APN	4	63,143,868 (GRCm39)	missense	probably benign	0.21
IGL02127:Col27a1	APN	4	63,143,379 (GRCm39)	missense	possibly damaging	0.60
IGL02290:Col27a1	APN	4	63,144,163 (GRCm39)	missense	probably damaging	1.00
IGL02374:Col27a1	APN	4	63,211,486 (GRCm39)	missense	possibly damaging	0.86
IGL02548:Col27a1	APN	4	63,236,492 (GRCm39)	splice site	probably benign
IGL02792:Col27a1	APN	4	63,233,820 (GRCm39)	missense	unknown
IGL02931:Col27a1	APN	4	63,249,663 (GRCm39)	utr 3 prime	probably benign
IGL03107:Col27a1	APN	4	63,242,869 (GRCm39)	splice site	probably benign
IGL03121:Col27a1	APN	4	63,143,446 (GRCm39)	missense	probably benign	0.26
IGL03334:Col27a1	APN	4	63,232,959 (GRCm39)	missense	probably damaging	1.00
R0005:Col27a1	UTSW	4	63,143,637 (GRCm39)	missense	probably benign	0.04
R0025:Col27a1	UTSW	4	63,194,214 (GRCm39)	missense	probably damaging	1.00
R0141:Col27a1	UTSW	4	63,183,870 (GRCm39)	critical splice acceptor site	probably null
R0196:Col27a1	UTSW	4	63,142,503 (GRCm39)	missense	probably benign	0.02
R0359:Col27a1	UTSW	4	63,232,964 (GRCm39)	critical splice donor site	probably null
R0375:Col27a1	UTSW	4	63,143,898 (GRCm39)	missense	probably benign	0.23
R0432:Col27a1	UTSW	4	63,143,848 (GRCm39)	missense	possibly damaging	0.87
R0499:Col27a1	UTSW	4	63,218,978 (GRCm39)	splice site	probably benign
R0786:Col27a1	UTSW	4	63,209,815 (GRCm39)	critical splice donor site	probably null
R0891:Col27a1	UTSW	4	63,223,420 (GRCm39)	critical splice acceptor site	probably null
R1239:Col27a1	UTSW	4	63,237,152 (GRCm39)	splice site	probably benign
R1297:Col27a1	UTSW	4	63,183,868 (GRCm39)	splice site	probably benign
R1299:Col27a1	UTSW	4	63,183,868 (GRCm39)	splice site	probably benign
R1322:Col27a1	UTSW	4	63,246,803 (GRCm39)	utr 3 prime	probably benign
R1342:Col27a1	UTSW	4	63,175,351 (GRCm39)	critical splice donor site	probably null
R1446:Col27a1	UTSW	4	63,143,040 (GRCm39)	missense	probably damaging	1.00
R1629:Col27a1	UTSW	4	63,248,100 (GRCm39)	utr 3 prime	probably benign
R1644:Col27a1	UTSW	4	63,246,868 (GRCm39)	utr 3 prime	probably benign
R1774:Col27a1	UTSW	4	63,143,950 (GRCm39)	missense	probably damaging	1.00
R1807:Col27a1	UTSW	4	63,249,586 (GRCm39)	utr 3 prime	probably benign
R1952:Col27a1	UTSW	4	63,202,130 (GRCm39)	splice site	probably null
R1957:Col27a1	UTSW	4	63,196,031 (GRCm39)	missense	probably benign	0.03
R2164:Col27a1	UTSW	4	63,143,661 (GRCm39)	missense	probably benign	0.21
R3774:Col27a1	UTSW	4	63,232,963 (GRCm39)	missense	probably benign	0.00
R4078:Col27a1	UTSW	4	63,142,669 (GRCm39)	missense	probably damaging	1.00
R4353:Col27a1	UTSW	4	63,143,868 (GRCm39)	missense	probably benign	0.21
R4611:Col27a1	UTSW	4	63,211,743 (GRCm39)	missense	probably damaging	1.00
R4708:Col27a1	UTSW	4	63,202,150 (GRCm39)	missense	probably benign	0.01
R4884:Col27a1	UTSW	4	63,194,197 (GRCm39)	missense	possibly damaging	0.77
R5149:Col27a1	UTSW	4	63,249,664 (GRCm39)	utr 3 prime	probably benign
R5411:Col27a1	UTSW	4	63,142,902 (GRCm39)	missense	probably damaging	1.00
R5451:Col27a1	UTSW	4	63,143,476 (GRCm39)	missense	probably damaging	0.98
R5615:Col27a1	UTSW	4	63,199,351 (GRCm39)	missense	probably damaging	0.96
R5657:Col27a1	UTSW	4	63,143,547 (GRCm39)	missense	probably damaging	0.97
R5838:Col27a1	UTSW	4	63,143,765 (GRCm39)	missense	probably damaging	1.00
R6230:Col27a1	UTSW	4	63,142,519 (GRCm39)	missense	probably damaging	1.00
R6326:Col27a1	UTSW	4	63,242,678 (GRCm39)	utr 3 prime	probably benign
R6457:Col27a1	UTSW	4	63,237,701 (GRCm39)	utr 3 prime	probably benign
R6624:Col27a1	UTSW	4	63,143,248 (GRCm39)	missense	probably benign	0.00
R6792:Col27a1	UTSW	4	63,235,740 (GRCm39)	missense	unknown
R6848:Col27a1	UTSW	4	63,220,608 (GRCm39)	missense	probably benign
R6962:Col27a1	UTSW	4	63,237,738 (GRCm39)	utr 3 prime	probably benign
R7053:Col27a1	UTSW	4	63,251,404 (GRCm39)	utr 3 prime	probably benign
R7206:Col27a1	UTSW	4	63,153,583 (GRCm39)	missense	probably benign	0.29
R7586:Col27a1	UTSW	4	63,143,278 (GRCm39)	missense	probably damaging	1.00
R7698:Col27a1	UTSW	4	63,143,955 (GRCm39)	missense	possibly damaging	0.78
R7714:Col27a1	UTSW	4	63,242,723 (GRCm39)	critical splice donor site	probably null
R7916:Col27a1	UTSW	4	63,142,789 (GRCm39)	missense	probably damaging	1.00
R7943:Col27a1	UTSW	4	63,236,520 (GRCm39)	missense	unknown
R7988:Col27a1	UTSW	4	63,249,559 (GRCm39)	missense	unknown
R8136:Col27a1	UTSW	4	63,202,190 (GRCm39)	missense	probably benign	0.06
R8243:Col27a1	UTSW	4	63,144,120 (GRCm39)	missense	probably damaging	1.00
R8245:Col27a1	UTSW	4	63,144,040 (GRCm39)	missense	probably damaging	0.97
R8350:Col27a1	UTSW	4	63,248,134 (GRCm39)	missense	unknown
R8437:Col27a1	UTSW	4	63,237,701 (GRCm39)	utr 3 prime	probably benign
R8450:Col27a1	UTSW	4	63,248,134 (GRCm39)	missense	unknown
R8542:Col27a1	UTSW	4	63,239,662 (GRCm39)	splice site	probably null
R8745:Col27a1	UTSW	4	63,144,153 (GRCm39)	missense	probably benign	0.02
R8821:Col27a1	UTSW	4	63,143,148 (GRCm39)	missense	probably benign	0.04
R8951:Col27a1	UTSW	4	63,191,311 (GRCm39)	missense	possibly damaging	0.92
R8970:Col27a1	UTSW	4	63,134,105 (GRCm39)	missense	unknown
R9115:Col27a1	UTSW	4	63,231,974 (GRCm39)	missense	unknown
R9185:Col27a1	UTSW	4	63,246,887 (GRCm39)	missense	unknown
R9291:Col27a1	UTSW	4	63,142,539 (GRCm39)	missense	probably damaging	0.99
R9404:Col27a1	UTSW	4	63,194,178 (GRCm39)	missense	possibly damaging	0.93
Z1176:Col27a1	UTSW	4	63,144,025 (GRCm39)	missense	probably damaging	0.99
Z1177:Col27a1	UTSW	4	63,199,526 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGAGGGTCACTGCAGCTTC -3'
(R):5'- TTTGAGATTCAGAGGTCAGCC -3'

Sequencing Primer
(F):5'- AGGGTCACTGCAGCTTCTCTTTG -3'
(R):5'- AGCCTTGGCTTCCCGTAGAC -3'

Posted On

2014-08-25