Mutagenetix > Incidental Mutation

Incidental Mutation 'R0138:Plce1'

22146

Institutional Source

Beutler Lab

Gene Symbol

Plce1

Ensembl Gene

ENSMUSG00000024998

Gene Name

phospholipase C, epsilon 1

Synonyms

PLCepsilon, 4933403A21Rik

MMRRC Submission

038423-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.488) question?

Stock #

R0138 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

38469557-38773474 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 38512863 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 54 (I54T)

Ref Sequence

ENSEMBL: ENSMUSP00000138360 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000169713] [ENSMUST00000182267] [ENSMUST00000182481]

AlphaFold

Q8K4S1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000169713
AA Change: I54T

PolyPhen 2 Score 0.488 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000130604
Gene: ENSMUSG00000024998
AA Change: I54T

Domain	Start	End	E-Value	Type
low complexity region	471	489	N/A	INTRINSIC
RasGEF	525	828	8.06e-9	SMART
low complexity region	1162	1172	N/A	INTRINSIC
Pfam:EF-hand_like	1305	1369	7.6e-11	PFAM
PLCXc	1373	1521	1.05e-81	SMART
low complexity region	1561	1575	N/A	INTRINSIC
SCOP:d1qasa3	1634	1662	1e-3	SMART
low complexity region	1666	1680	N/A	INTRINSIC
PLCYc	1710	1826	4.28e-46	SMART
C2	1850	1948	3.7e-10	SMART
PDB:2BYE\|A	1986	2094	6e-47	PDB
RA	2115	2218	1.12e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181994

Predicted Effect

probably benign
Transcript: ENSMUST00000182267
AA Change: I54T

PolyPhen 2 Score 0.276 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000138330
Gene: ENSMUSG00000024998
AA Change: I54T

Domain	Start	End	E-Value	Type
low complexity region	471	489	N/A	INTRINSIC
RasGEF	525	828	8.06e-9	SMART
low complexity region	1162	1172	N/A	INTRINSIC
Pfam:EF-hand_like	1305	1369	5.9e-11	PFAM
PLCXc	1373	1521	1.05e-81	SMART
low complexity region	1552	1581	N/A	INTRINSIC
SCOP:d1qasa3	1648	1676	1e-3	SMART
low complexity region	1680	1694	N/A	INTRINSIC
PLCYc	1724	1840	4.28e-46	SMART
C2	1864	1962	3.7e-10	SMART
PDB:2BYE\|A	2000	2108	6e-47	PDB
RA	2129	2232	1.12e-2	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000182481
AA Change: I54T

PolyPhen 2 Score 0.488 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000138360
Gene: ENSMUSG00000024998
AA Change: I54T

Domain	Start	End	E-Value	Type
low complexity region	471	489	N/A	INTRINSIC
RasGEF	525	828	8.06e-9	SMART
low complexity region	1162	1172	N/A	INTRINSIC
Pfam:EF-hand_like	1305	1369	8e-11	PFAM
PLCXc	1373	1521	1.05e-81	SMART
low complexity region	1561	1575	N/A	INTRINSIC
SCOP:d1qasa3	1634	1662	1e-3	SMART
low complexity region	1666	1680	N/A	INTRINSIC
PLCYc	1710	1826	4.28e-46	SMART
C2	1850	1948	3.7e-10	SMART
PDB:2BYE\|A	1986	2094	6e-47	PDB
RA	2115	2218	1.12e-2	SMART

Meta Mutation Damage Score

0.0855

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.4%
20x: 92.8%

Validation Efficiency

97% (76/78)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
PHENOTYPE: Homozygous mutation of this gene results in a congenital semilunar valvulogenesis defect which causes regurgitation and stenosis, and decreased incidence of induced skin tumors. Another mutant exhibits decreased cardiac contraction and increased hypertrophy in response to chronic stress. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	A	3: 121,899,098 (GRCm39)	N693K	probably damaging	Het
Adgrl3	T	A	5: 81,841,454 (GRCm39)	V845D	probably damaging	Het
Anxa8	T	A	14: 33,819,896 (GRCm39)	F269Y	probably benign	Het
Anxa8	T	A	14: 33,819,897 (GRCm39)	F295L	possibly damaging	Het
Aox4	C	G	1: 58,268,025 (GRCm39)	L202V	probably damaging	Het
Ap3s2	A	G	7: 79,559,617 (GRCm39)	V104A	probably benign	Het
Aqp3	G	A	4: 41,094,843 (GRCm39)		probably benign	Het
Arhgef26	C	T	3: 62,355,680 (GRCm39)	H751Y	probably benign	Het
Asic4	A	T	1: 75,446,331 (GRCm39)	Q291L	possibly damaging	Het
Bap1	T	C	14: 30,978,681 (GRCm39)	Y31H	probably damaging	Het
Brf1	A	T	12: 112,924,759 (GRCm39)	V655D	probably damaging	Het
Cebpz	A	G	17: 79,238,820 (GRCm39)	S663P	probably benign	Het
Ces2h	A	G	8: 105,744,693 (GRCm39)	D357G	probably benign	Het
Cfap36	T	C	11: 29,194,073 (GRCm39)	T90A	probably benign	Het
Ciita	A	T	16: 10,330,134 (GRCm39)	D803V	probably damaging	Het
Clnk	C	A	5: 38,931,951 (GRCm39)		probably benign	Het
Cyp46a1	A	G	12: 108,317,470 (GRCm39)	N158S	probably damaging	Het
Cyp4f13	A	G	17: 33,160,080 (GRCm39)	I98T	possibly damaging	Het
Def8	G	A	8: 124,183,234 (GRCm39)	A278T	probably damaging	Het
Dll3	T	A	7: 28,000,746 (GRCm39)	D103V	possibly damaging	Het
Dnai1	T	A	4: 41,629,814 (GRCm39)	M446K	possibly damaging	Het
Dppa4	A	T	16: 48,111,425 (GRCm39)	T85S	probably benign	Het
Eif4g1	A	T	16: 20,494,095 (GRCm39)	H57L	probably damaging	Het
Fmnl3	G	C	15: 99,220,619 (GRCm39)		probably benign	Het
Fn1	T	A	1: 71,663,269 (GRCm39)	Q1073L	possibly damaging	Het
Foxp4	T	C	17: 48,180,104 (GRCm39)	D599G	unknown	Het
Frrs1	T	C	3: 116,675,456 (GRCm39)	V128A	possibly damaging	Het
Gcfc2	G	A	6: 81,926,935 (GRCm39)	D608N	probably damaging	Het
Gm1043	T	C	5: 37,350,317 (GRCm39)		probably benign	Het
Gm5148	T	C	3: 37,768,926 (GRCm39)	E98G	probably benign	Het
Gpr141	T	C	13: 19,936,428 (GRCm39)	I116V	probably benign	Het
Hic1	T	C	11: 75,058,169 (GRCm39)	N240S	probably damaging	Het
Hpx	G	A	7: 105,241,445 (GRCm39)	T322I	probably damaging	Het
Hs3st4	A	T	7: 123,996,416 (GRCm39)	M361L	probably benign	Het
Ifrd1	A	G	12: 40,257,129 (GRCm39)		probably benign	Het
Ino80	G	A	2: 119,213,441 (GRCm39)	R1249C	probably damaging	Het
Klk1b21	T	A	7: 43,755,319 (GRCm39)	C173S	probably damaging	Het
Krt25	A	T	11: 99,213,524 (GRCm39)	V65E	probably benign	Het
Lrrc15	A	T	16: 30,092,267 (GRCm39)	D357E	possibly damaging	Het
Lrrd1	T	A	5: 3,901,345 (GRCm39)	V550E	probably benign	Het
Macf1	A	G	4: 123,334,540 (GRCm39)	Y1490H	probably damaging	Het
Macrod1	A	G	19: 7,174,281 (GRCm39)		probably benign	Het
Mcm5	T	A	8: 75,847,508 (GRCm39)	V435D	probably damaging	Het
Mctp1	C	T	13: 76,975,831 (GRCm39)	R478C	probably damaging	Het
Med10	T	C	13: 69,959,817 (GRCm39)		probably benign	Het
Mrpl4	T	C	9: 20,919,888 (GRCm39)	Y280H	probably benign	Het
Msrb3	T	C	10: 120,687,892 (GRCm39)	E61G	probably damaging	Het
Myo1c	T	C	11: 75,551,827 (GRCm39)	Y337H	possibly damaging	Het
Myo7b	T	A	18: 32,143,204 (GRCm39)	T165S	probably damaging	Het
Myrfl	T	A	10: 116,685,138 (GRCm39)	R81W	probably damaging	Het
Neil1	T	C	9: 57,051,030 (GRCm39)		probably benign	Het
Neto2	A	G	8: 86,367,673 (GRCm39)	I357T	possibly damaging	Het
Nfat5	C	T	8: 108,065,707 (GRCm39)	R156W	probably damaging	Het
Nkx6-3	T	C	8: 23,643,607 (GRCm39)	S3P	probably benign	Het
Or52h7	A	T	7: 104,214,210 (GRCm39)	I261L	probably benign	Het
Prex2	A	T	1: 11,355,267 (GRCm39)		probably benign	Het
Psapl1	T	A	5: 36,361,975 (GRCm39)	V189E	probably damaging	Het
Ptdss2	T	G	7: 140,735,232 (GRCm39)		probably benign	Het
Rnf213	T	C	11: 119,307,322 (GRCm39)	C661R	probably benign	Het
Rpap1	T	C	2: 119,595,380 (GRCm39)		probably null	Het
Rrp1b	A	G	17: 32,279,426 (GRCm39)	T696A	probably benign	Het
Sacm1l	T	A	9: 123,377,982 (GRCm39)	H87Q	probably benign	Het
Serpinb11	T	A	1: 107,305,260 (GRCm39)	M212K	probably damaging	Het
Tbc1d22a	C	A	15: 86,183,885 (GRCm39)	T248K	probably damaging	Het
Tcerg1	C	T	18: 42,701,679 (GRCm39)		probably benign	Het
Tpst1	T	A	5: 130,130,627 (GRCm39)	H32Q	probably damaging	Het
Tsc2	A	T	17: 24,818,600 (GRCm39)	V1412E	possibly damaging	Het
Usp19	C	A	9: 108,378,514 (GRCm39)	P1326Q	possibly damaging	Het
Vmn1r235	T	A	17: 21,482,596 (GRCm39)	M307K	probably damaging	Het
Vmn2r58	T	A	7: 41,487,048 (GRCm39)	T616S	probably damaging	Het
Vps13a	G	A	19: 16,637,863 (GRCm39)	T2406I	possibly damaging	Het
Zbtb26	T	A	2: 37,326,053 (GRCm39)	M328L	probably benign	Het
Zp2	A	G	7: 119,736,423 (GRCm39)	F340S	probably damaging	Het

Other mutations in Plce1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00090:Plce1	APN	19	38,734,232 (GRCm39)	missense	probably damaging	0.99
IGL00336:Plce1	APN	19	38,640,350 (GRCm39)	missense	probably damaging	1.00
IGL00430:Plce1	APN	19	38,713,461 (GRCm39)	missense	probably damaging	1.00
IGL00466:Plce1	APN	19	38,709,473 (GRCm39)	missense	probably damaging	0.99
IGL00477:Plce1	APN	19	38,513,576 (GRCm39)	missense	probably benign	0.39
IGL00839:Plce1	APN	19	38,687,006 (GRCm39)	missense	probably damaging	1.00
IGL01292:Plce1	APN	19	38,640,229 (GRCm39)	splice site	probably benign
IGL01665:Plce1	APN	19	38,513,331 (GRCm39)	missense	probably benign	0.01
IGL01826:Plce1	APN	19	38,727,682 (GRCm39)	splice site	probably benign
IGL01833:Plce1	APN	19	38,709,425 (GRCm39)	missense	probably damaging	1.00
IGL02201:Plce1	APN	19	38,757,890 (GRCm39)	splice site	probably benign
IGL02276:Plce1	APN	19	38,513,201 (GRCm39)	missense	probably benign	0.05
IGL02477:Plce1	APN	19	38,707,997 (GRCm39)	splice site	probably benign
IGL02746:Plce1	APN	19	38,686,916 (GRCm39)	missense	probably damaging	1.00
Angel_food	UTSW	19	38,715,457 (GRCm39)	splice site	probably benign
Heavenly	UTSW	19	38,766,433 (GRCm39)	missense	probably damaging	1.00
R0058:Plce1	UTSW	19	38,513,628 (GRCm39)	missense	possibly damaging	0.90
R0058:Plce1	UTSW	19	38,513,628 (GRCm39)	missense	possibly damaging	0.90
R0064:Plce1	UTSW	19	38,769,228 (GRCm39)	critical splice donor site	probably null
R0116:Plce1	UTSW	19	38,710,265 (GRCm39)	missense	probably benign
R0240:Plce1	UTSW	19	38,717,330 (GRCm39)	missense	probably damaging	0.99
R0240:Plce1	UTSW	19	38,717,330 (GRCm39)	missense	probably damaging	0.99
R0504:Plce1	UTSW	19	38,766,465 (GRCm39)	splice site	probably benign
R0506:Plce1	UTSW	19	38,748,582 (GRCm39)	missense	probably benign	0.04
R0578:Plce1	UTSW	19	38,766,383 (GRCm39)	missense	probably damaging	1.00
R0645:Plce1	UTSW	19	38,766,433 (GRCm39)	missense	probably damaging	1.00
R0730:Plce1	UTSW	19	38,705,135 (GRCm39)	missense	probably damaging	0.98
R0920:Plce1	UTSW	19	38,724,965 (GRCm39)	missense	probably damaging	1.00
R1223:Plce1	UTSW	19	38,755,670 (GRCm39)	missense	probably damaging	1.00
R1223:Plce1	UTSW	19	38,690,457 (GRCm39)	missense	probably damaging	1.00
R1484:Plce1	UTSW	19	38,693,783 (GRCm39)	nonsense	probably null
R1488:Plce1	UTSW	19	38,705,247 (GRCm39)	missense	possibly damaging	0.92
R1598:Plce1	UTSW	19	38,709,440 (GRCm39)	missense	probably damaging	1.00
R1624:Plce1	UTSW	19	38,713,219 (GRCm39)	missense	probably damaging	1.00
R1732:Plce1	UTSW	19	38,705,282 (GRCm39)	missense	possibly damaging	0.56
R1778:Plce1	UTSW	19	38,769,234 (GRCm39)	splice site	probably benign
R1797:Plce1	UTSW	19	38,747,392 (GRCm39)	critical splice donor site	probably null
R1872:Plce1	UTSW	19	38,748,521 (GRCm39)	missense	probably damaging	1.00
R1876:Plce1	UTSW	19	38,769,067 (GRCm39)	missense	probably damaging	1.00
R1991:Plce1	UTSW	19	38,766,368 (GRCm39)	missense	probably damaging	1.00
R2080:Plce1	UTSW	19	38,715,457 (GRCm39)	splice site	probably benign
R2103:Plce1	UTSW	19	38,766,368 (GRCm39)	missense	probably damaging	1.00
R2376:Plce1	UTSW	19	38,766,430 (GRCm39)	missense	probably benign	0.02
R2471:Plce1	UTSW	19	38,768,370 (GRCm39)	missense	probably damaging	1.00
R2511:Plce1	UTSW	19	38,748,498 (GRCm39)	missense	probably damaging	1.00
R2842:Plce1	UTSW	19	38,512,727 (GRCm39)	missense	probably damaging	1.00
R3037:Plce1	UTSW	19	38,766,328 (GRCm39)	missense	probably damaging	0.98
R3104:Plce1	UTSW	19	38,608,963 (GRCm39)	missense	probably benign	0.00
R3700:Plce1	UTSW	19	38,693,781 (GRCm39)	missense	probably damaging	1.00
R3750:Plce1	UTSW	19	38,766,343 (GRCm39)	missense	probably benign
R3753:Plce1	UTSW	19	38,640,278 (GRCm39)	missense	probably benign	0.09
R4027:Plce1	UTSW	19	38,512,709 (GRCm39)	missense	probably damaging	1.00
R4057:Plce1	UTSW	19	38,748,563 (GRCm39)	missense	probably damaging	1.00
R4376:Plce1	UTSW	19	38,693,891 (GRCm39)	critical splice donor site	probably null
R4433:Plce1	UTSW	19	38,755,745 (GRCm39)	missense	probably damaging	1.00
R4520:Plce1	UTSW	19	38,512,763 (GRCm39)	missense	possibly damaging	0.46
R4521:Plce1	UTSW	19	38,512,763 (GRCm39)	missense	possibly damaging	0.46
R4522:Plce1	UTSW	19	38,512,763 (GRCm39)	missense	possibly damaging	0.46
R4524:Plce1	UTSW	19	38,512,763 (GRCm39)	missense	possibly damaging	0.46
R4650:Plce1	UTSW	19	38,513,088 (GRCm39)	missense	probably benign	0.30
R4673:Plce1	UTSW	19	38,737,840 (GRCm39)	missense	possibly damaging	0.51
R4701:Plce1	UTSW	19	38,713,451 (GRCm39)	missense	probably benign	0.33
R4828:Plce1	UTSW	19	38,757,943 (GRCm39)	missense	probably damaging	1.00
R5103:Plce1	UTSW	19	38,755,659 (GRCm39)	missense	probably damaging	1.00
R5112:Plce1	UTSW	19	38,640,277 (GRCm39)	missense	probably benign	0.00
R5236:Plce1	UTSW	19	38,758,791 (GRCm39)	missense	probably benign	0.11
R5268:Plce1	UTSW	19	38,747,279 (GRCm39)	missense	possibly damaging	0.71
R5288:Plce1	UTSW	19	38,748,535 (GRCm39)	missense	probably damaging	1.00
R5384:Plce1	UTSW	19	38,748,535 (GRCm39)	missense	probably damaging	1.00
R5386:Plce1	UTSW	19	38,748,535 (GRCm39)	missense	probably damaging	1.00
R5448:Plce1	UTSW	19	38,768,361 (GRCm39)	missense	probably damaging	1.00
R5452:Plce1	UTSW	19	38,608,926 (GRCm39)	missense	probably benign	0.01
R6004:Plce1	UTSW	19	38,710,315 (GRCm39)	missense	probably damaging	1.00
R6062:Plce1	UTSW	19	38,513,195 (GRCm39)	missense	probably benign
R6147:Plce1	UTSW	19	38,690,481 (GRCm39)	missense	probably damaging	1.00
R6247:Plce1	UTSW	19	38,734,289 (GRCm39)	missense	probably damaging	1.00
R6278:Plce1	UTSW	19	38,713,495 (GRCm39)	splice site	probably null
R6306:Plce1	UTSW	19	38,757,909 (GRCm39)	missense	probably damaging	1.00
R6317:Plce1	UTSW	19	38,512,974 (GRCm39)	nonsense	probably null
R6437:Plce1	UTSW	19	38,513,576 (GRCm39)	missense	probably benign	0.39
R6522:Plce1	UTSW	19	38,736,965 (GRCm39)	splice site	probably null
R7034:Plce1	UTSW	19	38,727,801 (GRCm39)	missense	probably damaging	1.00
R7036:Plce1	UTSW	19	38,727,801 (GRCm39)	missense	probably damaging	1.00
R7037:Plce1	UTSW	19	38,690,461 (GRCm39)	missense	probably damaging	1.00
R7069:Plce1	UTSW	19	38,747,384 (GRCm39)	missense	probably damaging	1.00
R7180:Plce1	UTSW	19	38,768,229 (GRCm39)	missense	probably damaging	1.00
R7189:Plce1	UTSW	19	38,748,581 (GRCm39)	missense	probably damaging	0.97
R7227:Plce1	UTSW	19	38,715,346 (GRCm39)	missense	probably benign	0.00
R7253:Plce1	UTSW	19	38,686,952 (GRCm39)	missense	probably damaging	1.00
R7278:Plce1	UTSW	19	38,768,340 (GRCm39)	missense	possibly damaging	0.58
R7287:Plce1	UTSW	19	38,690,347 (GRCm39)	missense	probably benign	0.02
R7422:Plce1	UTSW	19	38,640,329 (GRCm39)	missense	probably damaging	1.00
R7557:Plce1	UTSW	19	38,753,848 (GRCm39)	missense	probably benign	0.30
R7607:Plce1	UTSW	19	38,513,196 (GRCm39)	missense	probably benign
R7615:Plce1	UTSW	19	38,513,109 (GRCm39)	missense	probably benign	0.18
R7653:Plce1	UTSW	19	38,737,763 (GRCm39)	missense	probably benign	0.20
R7685:Plce1	UTSW	19	38,736,877 (GRCm39)	missense	probably benign	0.00
R7716:Plce1	UTSW	19	38,705,295 (GRCm39)	missense	probably benign
R7744:Plce1	UTSW	19	38,608,899 (GRCm39)	missense	possibly damaging	0.93
R7790:Plce1	UTSW	19	38,769,140 (GRCm39)	missense	probably damaging	0.97
R7921:Plce1	UTSW	19	38,608,997 (GRCm39)	missense	probably benign	0.03
R8070:Plce1	UTSW	19	38,690,283 (GRCm39)	missense	probably damaging	0.99
R8087:Plce1	UTSW	19	38,724,965 (GRCm39)	missense	probably damaging	1.00
R8116:Plce1	UTSW	19	38,513,262 (GRCm39)	missense	probably benign	0.32
R8178:Plce1	UTSW	19	38,761,423 (GRCm39)	missense	possibly damaging	0.93
R8321:Plce1	UTSW	19	38,640,380 (GRCm39)	missense	probably benign	0.00
R8416:Plce1	UTSW	19	38,761,441 (GRCm39)	missense	possibly damaging	0.77
R8544:Plce1	UTSW	19	38,512,903 (GRCm39)	missense	probably benign	0.00
R8713:Plce1	UTSW	19	38,513,345 (GRCm39)	missense	probably benign	0.01
R8850:Plce1	UTSW	19	38,512,811 (GRCm39)	missense	probably benign
R9217:Plce1	UTSW	19	38,748,551 (GRCm39)	missense	probably damaging	1.00
R9231:Plce1	UTSW	19	38,705,040 (GRCm39)	missense	probably benign	0.13
R9232:Plce1	UTSW	19	38,705,423 (GRCm39)	missense	probably benign	0.16
R9332:Plce1	UTSW	19	38,726,377 (GRCm39)	missense	probably damaging	1.00
R9473:Plce1	UTSW	19	38,766,337 (GRCm39)	missense	possibly damaging	0.93
R9474:Plce1	UTSW	19	38,766,337 (GRCm39)	missense	possibly damaging	0.93
R9475:Plce1	UTSW	19	38,766,337 (GRCm39)	missense	possibly damaging	0.93
R9476:Plce1	UTSW	19	38,766,337 (GRCm39)	missense	possibly damaging	0.93
R9751:Plce1	UTSW	19	38,717,414 (GRCm39)	missense	probably damaging	1.00
R9780:Plce1	UTSW	19	38,609,134 (GRCm39)	missense	possibly damaging	0.94
R9781:Plce1	UTSW	19	38,513,654 (GRCm39)	missense	probably damaging	1.00
RF018:Plce1	UTSW	19	38,705,651 (GRCm39)	missense	probably damaging	0.99
X0022:Plce1	UTSW	19	38,715,443 (GRCm39)	missense	probably damaging	1.00
X0065:Plce1	UTSW	19	38,766,358 (GRCm39)	missense	possibly damaging	0.48
Z1176:Plce1	UTSW	19	38,757,904 (GRCm39)	missense	probably damaging	1.00
Z1176:Plce1	UTSW	19	38,713,424 (GRCm39)	nonsense	probably null
Z1176:Plce1	UTSW	19	38,690,338 (GRCm39)	missense	probably damaging	1.00
Z1177:Plce1	UTSW	19	38,640,286 (GRCm39)	missense	probably null	0.48

Predicted Primers

PCR Primer
(F):5'- GCTTGCTTCGCACAAAGACTGG -3'
(R):5'- CACTAAACTGTTGCCGTTCATCGC -3'

Sequencing Primer
(F):5'- CGCACAAAGACTGGAATTATCTG -3'
(R):5'- CGTTGGAATTCTCATCGCAG -3'

Posted On

2013-04-12