Mutagenetix > Incidental Mutation

Incidental Mutation 'R2045:Vcan'

221865

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, heart defect, Cspg2, DPEAAE, 5430420N07Rik

MMRRC Submission

040052-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2045 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

89803431-89890628 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 89839104 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 2147 (I2147L)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109544

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109546
AA Change: I2147L

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: I2147L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159910
AA Change: I1187L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614
AA Change: I1187L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.0%
20x: 94.6%

Validation Efficiency

100% (74/74)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acta2	C	T	19: 34,220,799 (GRCm39)	G303E	probably damaging	Het
Adamts1	A	T	16: 85,592,864 (GRCm39)	Y515N	probably damaging	Het
Ankef1	T	C	2: 136,396,658 (GRCm39)	V695A	probably benign	Het
Ankrd63	C	A	2: 118,533,834 (GRCm39)		probably benign	Het
Asah2	A	T	19: 32,030,356 (GRCm39)	N105K	probably benign	Het
Atf2	A	C	2: 73,693,552 (GRCm39)	D3E	probably damaging	Het
Atp5pb	T	C	3: 105,851,190 (GRCm39)		probably benign	Het
Bag4	C	T	8: 26,259,516 (GRCm39)	A228T	probably benign	Het
Bms1	A	C	6: 118,369,588 (GRCm39)	L960W	probably damaging	Het
Cacna1c	A	G	6: 118,633,098 (GRCm39)	V977A	probably damaging	Het
Cadps2	A	G	6: 23,839,121 (GRCm39)	S6P	possibly damaging	Het
Capn8	A	G	1: 182,440,951 (GRCm39)	T462A	probably benign	Het
Cd226	T	C	18: 89,225,486 (GRCm39)	S128P	probably benign	Het
Cd33	G	T	7: 43,179,316 (GRCm39)	H278N	probably benign	Het
Cdh1	T	C	8: 107,392,814 (GRCm39)		probably benign	Het
Cfap54	T	C	10: 92,874,671 (GRCm39)		probably null	Het
Chit1	A	G	1: 134,078,882 (GRCm39)	I397M	probably benign	Het
Cic	C	A	7: 24,970,961 (GRCm39)	Q231K	possibly damaging	Het
Clca4b	A	G	3: 144,630,924 (GRCm39)	V312A	probably damaging	Het
Cngb1	T	A	8: 96,023,713 (GRCm39)		probably null	Het
Cyfip2	T	C	11: 46,140,616 (GRCm39)	I430V	probably benign	Het
Dnah12	A	G	14: 26,503,485 (GRCm39)	E1613G	probably null	Het
Dock2	T	C	11: 34,244,106 (GRCm39)		probably null	Het
Dync2h1	A	T	9: 7,160,171 (GRCm39)	F646I	probably damaging	Het
Eef1b2	A	T	1: 63,218,646 (GRCm39)	K144*	probably null	Het
Erap1	T	C	13: 74,817,569 (GRCm39)	V137A	probably benign	Het
Far1	A	T	7: 113,138,478 (GRCm39)		probably null	Het
Fbn2	A	T	18: 58,223,730 (GRCm39)	C807S	probably damaging	Het
Fgfr1	A	G	8: 26,048,231 (GRCm39)	K209R	probably benign	Het
Fpr-rs4	CAGGAA	CA	17: 18,242,596 (GRCm39)		probably null	Het
Frem2	A	T	3: 53,443,165 (GRCm39)	V2533D	probably damaging	Het
Hip1r	T	C	5: 124,138,794 (GRCm39)	M839T	probably benign	Het
Igfbp2	A	G	1: 72,891,310 (GRCm39)	S303G	probably benign	Het
Insyn2a	T	G	7: 134,520,159 (GRCm39)	K124Q	probably damaging	Het
Itga10	C	T	3: 96,559,054 (GRCm39)		probably benign	Het
Itgb3	T	G	11: 104,514,239 (GRCm39)	S27A	probably benign	Het
Kif21b	G	A	1: 136,088,051 (GRCm39)	D1015N	probably damaging	Het
Krt7	A	T	15: 101,321,365 (GRCm39)		probably null	Het
Krtdap	T	A	7: 30,490,010 (GRCm39)	F80L	probably benign	Het
Lcp1	A	T	14: 75,437,841 (GRCm39)	T84S	probably benign	Het
Lipi	G	A	16: 75,347,087 (GRCm39)	T444I	probably damaging	Het
Lrguk	A	T	6: 34,048,003 (GRCm39)	E316V	probably damaging	Het
Lypd1	G	A	1: 125,838,272 (GRCm39)		probably benign	Het
Med12l	A	T	3: 59,169,731 (GRCm39)	K1632*	probably null	Het
Mrgpra9	A	T	7: 46,885,583 (GRCm39)	M28K	probably benign	Het
Mylk	A	G	16: 34,774,023 (GRCm39)	K1291E	probably benign	Het
Nek4	T	A	14: 30,675,880 (GRCm39)	W72R	probably damaging	Het
Nudt8	T	C	19: 4,051,899 (GRCm39)	V170A	probably damaging	Het
Oosp3	T	A	19: 11,676,733 (GRCm39)	Y31N	probably benign	Het
Padi2	A	T	4: 140,665,241 (GRCm39)	R449W	probably damaging	Het
Pcf11	G	T	7: 92,311,087 (GRCm39)	N300K	probably damaging	Het
Pcsk5	T	C	19: 17,558,508 (GRCm39)	D633G	possibly damaging	Het
Phlpp2	T	A	8: 110,634,232 (GRCm39)	W271R	probably damaging	Het
Pikfyve	T	C	1: 65,292,512 (GRCm39)	V1276A	probably benign	Het
Pkhd1l1	A	T	15: 44,343,050 (GRCm39)	N176Y	probably damaging	Het
Pop5	T	G	5: 115,376,271 (GRCm39)	V33G	possibly damaging	Het
Prkag2	A	G	5: 25,152,580 (GRCm39)	F175L	possibly damaging	Het
Ptpn22	T	A	3: 103,781,337 (GRCm39)	D79E	possibly damaging	Het
Rab32	T	G	10: 10,426,577 (GRCm39)	D123A	probably damaging	Het
Rnpc3	T	C	3: 113,402,009 (GRCm39)	K513E	possibly damaging	Het
Senp1	A	T	15: 97,957,825 (GRCm39)	F358I	possibly damaging	Het
Sft2d2	A	G	1: 165,012,647 (GRCm39)	L83P	probably damaging	Het
Slc9c1	G	A	16: 45,400,613 (GRCm39)	R741H	probably damaging	Het
Smad4	A	T	18: 73,782,877 (GRCm39)	Y352*	probably null	Het
Tamm41	T	A	6: 114,993,056 (GRCm39)	Q232H	probably benign	Het
Tbx6	T	A	7: 126,382,055 (GRCm39)	L131Q	probably damaging	Het
Trappc10	T	C	10: 78,045,313 (GRCm39)		probably benign	Het
Trp53bp1	C	A	2: 121,034,964 (GRCm39)	A108S	probably benign	Het
Tut7	T	C	13: 59,948,470 (GRCm39)	Y215C	probably damaging	Het
Unc13b	G	A	4: 43,091,266 (GRCm39)	V31M	probably damaging	Het
Usp24	T	C	4: 106,258,177 (GRCm39)	M1525T	possibly damaging	Het
Vax2	G	A	6: 83,688,252 (GRCm39)		probably benign	Het
Zbtb7a	C	A	10: 80,980,244 (GRCm39)	A146E	probably benign	Het
Zfp287	A	G	11: 62,618,395 (GRCm39)	L157P	probably damaging	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,852,821 (GRCm39)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,840,438 (GRCm39)	missense	probably benign
IGL00504:Vcan	APN	13	89,839,394 (GRCm39)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,837,098 (GRCm39)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,851,845 (GRCm39)	missense	probably benign
IGL00743:Vcan	APN	13	89,873,425 (GRCm39)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,810,171 (GRCm39)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,828,077 (GRCm39)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,839,787 (GRCm39)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,852,062 (GRCm39)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,852,288 (GRCm39)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,836,794 (GRCm39)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,838,705 (GRCm39)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,837,864 (GRCm39)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,873,507 (GRCm39)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,837,324 (GRCm39)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,837,478 (GRCm39)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,832,557 (GRCm39)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,873,684 (GRCm39)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,832,612 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,851,196 (GRCm39)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,852,968 (GRCm39)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,838,776 (GRCm39)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,836,742 (GRCm39)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,851,394 (GRCm39)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,810,051 (GRCm39)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,853,125 (GRCm39)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,810,104 (GRCm39)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,826,192 (GRCm39)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,839,380 (GRCm39)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,860,310 (GRCm39)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,839,394 (GRCm39)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,851,665 (GRCm39)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,851,318 (GRCm39)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,826,264 (GRCm39)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,852,779 (GRCm39)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,851,891 (GRCm39)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,879,583 (GRCm39)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,860,372 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,860,386 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,852,825 (GRCm39)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,827,941 (GRCm39)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,853,072 (GRCm39)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,833,196 (GRCm39)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,840,529 (GRCm39)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,852,422 (GRCm39)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,827,913 (GRCm39)	splice site	probably null
R1219:Vcan	UTSW	13	89,828,023 (GRCm39)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,805,675 (GRCm39)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,841,075 (GRCm39)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,837,780 (GRCm39)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,851,786 (GRCm39)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,810,065 (GRCm39)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,851,666 (GRCm39)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,836,602 (GRCm39)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,869,894 (GRCm39)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,852,854 (GRCm39)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,839,800 (GRCm39)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,853,331 (GRCm39)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,853,511 (GRCm39)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,838,990 (GRCm39)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,841,134 (GRCm39)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,853,653 (GRCm39)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,851,045 (GRCm39)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,851,543 (GRCm39)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,840,861 (GRCm39)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,837,157 (GRCm39)	missense	probably damaging	1.00
R2110:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,837,856 (GRCm39)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,851,648 (GRCm39)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,851,529 (GRCm39)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,837,568 (GRCm39)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,852,794 (GRCm39)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,852,356 (GRCm39)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,851,420 (GRCm39)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,828,006 (GRCm39)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,833,277 (GRCm39)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,873,666 (GRCm39)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,873,605 (GRCm39)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,852,147 (GRCm39)	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89,828,053 (GRCm39)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,853,908 (GRCm39)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,852,858 (GRCm39)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,836,961 (GRCm39)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,852,096 (GRCm39)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,805,591 (GRCm39)	intron	probably benign
R5124:Vcan	UTSW	13	89,873,636 (GRCm39)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,838,359 (GRCm39)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,838,991 (GRCm39)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,840,651 (GRCm39)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,839,719 (GRCm39)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,838,405 (GRCm39)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,839,929 (GRCm39)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,851,231 (GRCm39)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,836,790 (GRCm39)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,839,622 (GRCm39)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,839,254 (GRCm39)	frame shift	probably null
R5687:Vcan	UTSW	13	89,826,253 (GRCm39)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,828,069 (GRCm39)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,852,071 (GRCm39)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,840,810 (GRCm39)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,805,655 (GRCm39)	nonsense	probably null
R6135:Vcan	UTSW	13	89,838,045 (GRCm39)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,839,339 (GRCm39)	nonsense	probably null
R6280:Vcan	UTSW	13	89,873,492 (GRCm39)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,839,716 (GRCm39)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,852,951 (GRCm39)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,838,806 (GRCm39)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,852,850 (GRCm39)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,853,301 (GRCm39)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,853,244 (GRCm39)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,860,500 (GRCm39)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,837,550 (GRCm39)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,826,252 (GRCm39)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,828,075 (GRCm39)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,841,526 (GRCm39)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,838,737 (GRCm39)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,838,360 (GRCm39)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,853,805 (GRCm39)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,837,229 (GRCm39)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,873,710 (GRCm39)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,837,055 (GRCm39)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,853,389 (GRCm39)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,879,576 (GRCm39)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,869,805 (GRCm39)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,853,280 (GRCm39)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,840,852 (GRCm39)	nonsense	probably null
R7299:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7301:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7425:Vcan	UTSW	13	89,837,951 (GRCm39)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,852,237 (GRCm39)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,840,577 (GRCm39)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,840,342 (GRCm39)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,839,908 (GRCm39)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,852,738 (GRCm39)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,837,442 (GRCm39)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,841,056 (GRCm39)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,839,977 (GRCm39)	missense	probably benign
R7998:Vcan	UTSW	13	89,852,446 (GRCm39)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,852,479 (GRCm39)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,805,409 (GRCm39)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,851,439 (GRCm39)	nonsense	probably null
R8103:Vcan	UTSW	13	89,805,777 (GRCm39)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,852,373 (GRCm39)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,853,106 (GRCm39)	nonsense	probably null
R8166:Vcan	UTSW	13	89,840,855 (GRCm39)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,853,089 (GRCm39)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,852,454 (GRCm39)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,836,862 (GRCm39)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,839,217 (GRCm39)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,840,439 (GRCm39)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,840,230 (GRCm39)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,853,026 (GRCm39)	missense	probably benign
R9049:Vcan	UTSW	13	89,826,224 (GRCm39)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,839,146 (GRCm39)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,852,644 (GRCm39)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,828,050 (GRCm39)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,838,615 (GRCm39)	nonsense	probably null
R9259:Vcan	UTSW	13	89,838,989 (GRCm39)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,837,452 (GRCm39)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,851,531 (GRCm39)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,841,128 (GRCm39)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,839,659 (GRCm39)	missense
R9579:Vcan	UTSW	13	89,837,713 (GRCm39)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,853,491 (GRCm39)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,841,081 (GRCm39)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,839,860 (GRCm39)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,839,247 (GRCm39)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,837,930 (GRCm39)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,840,612 (GRCm39)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,853,868 (GRCm39)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,840,690 (GRCm39)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,852,192 (GRCm39)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,851,907 (GRCm39)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,851,643 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GTTTCACACCCTTTTGGAAGAG -3'
(R):5'- ACGATGTCACTGGACTTTCCC -3'

Sequencing Primer
(F):5'- CACACCCTTTTGGAAGAGTTTTATGC -3'
(R):5'- TGGACTTTCCCCAGACAATG -3'

Posted On

2014-08-25