Incidental Mutation 'R1980:Zfp329'
ID 222140
Institutional Source Beutler Lab
Gene Symbol Zfp329
Ensembl Gene ENSMUSG00000057894
Gene Name zinc finger protein 329
Synonyms 4632409L22Rik, 2810439M05Rik
MMRRC Submission 039992-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R1980 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 12538904-12552785 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 12545395 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Isoleucine at position 43 (N43I)
Ref Sequence ENSEMBL: ENSMUSP00000113355 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000072222] [ENSMUST00000108546] [ENSMUST00000121215]
AlphaFold Q6GQR8
Predicted Effect probably benign
Transcript: ENSMUST00000072222
AA Change: N43I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000072079
Gene: ENSMUSG00000057894
AA Change: N43I

DomainStartEndE-ValueType
low complexity region 147 160 N/A INTRINSIC
ZnF_C2H2 184 206 9.58e-3 SMART
ZnF_C2H2 212 234 1.12e-3 SMART
ZnF_C2H2 240 262 1.22e-4 SMART
ZnF_C2H2 268 290 1.95e-3 SMART
ZnF_C2H2 296 318 2.61e-4 SMART
ZnF_C2H2 324 346 5.14e-3 SMART
ZnF_C2H2 352 374 2.24e-3 SMART
ZnF_C2H2 380 402 5.21e-4 SMART
ZnF_C2H2 408 430 1.92e-2 SMART
ZnF_C2H2 436 458 5.21e-4 SMART
ZnF_C2H2 464 486 3.16e-3 SMART
ZnF_C2H2 492 514 2.2e-2 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108546
AA Change: N43I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000104186
Gene: ENSMUSG00000057894
AA Change: N43I

DomainStartEndE-ValueType
low complexity region 147 160 N/A INTRINSIC
ZnF_C2H2 184 206 9.58e-3 SMART
ZnF_C2H2 212 234 1.12e-3 SMART
ZnF_C2H2 240 262 1.22e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000121215
AA Change: N43I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000113355
Gene: ENSMUSG00000057894
AA Change: N43I

DomainStartEndE-ValueType
low complexity region 147 160 N/A INTRINSIC
ZnF_C2H2 184 206 9.58e-3 SMART
ZnF_C2H2 212 234 1.12e-3 SMART
ZnF_C2H2 240 262 1.22e-4 SMART
ZnF_C2H2 268 290 1.95e-3 SMART
ZnF_C2H2 296 318 2.61e-4 SMART
ZnF_C2H2 324 346 5.14e-3 SMART
ZnF_C2H2 352 374 2.24e-3 SMART
ZnF_C2H2 380 402 5.21e-4 SMART
ZnF_C2H2 408 430 1.92e-2 SMART
ZnF_C2H2 436 458 5.21e-4 SMART
ZnF_C2H2 464 486 3.16e-3 SMART
ZnF_C2H2 492 514 2.2e-2 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000129647
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.8%
  • 20x: 94.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acly A C 11: 100,386,702 (GRCm39) I620S possibly damaging Het
Acot8 A G 2: 164,636,964 (GRCm39) F262S probably damaging Het
Adgb C T 10: 10,309,242 (GRCm39) V246I probably benign Het
Akap9 T A 5: 4,022,771 (GRCm39) M1200K probably damaging Het
Alg11 T A 8: 22,551,903 (GRCm39) F16I possibly damaging Het
Apol7c A G 15: 77,410,244 (GRCm39) V234A probably benign Het
Arhgap19 T G 19: 41,776,784 (GRCm39) I122L possibly damaging Het
Arhgef37 A G 18: 61,641,767 (GRCm39) S201P probably damaging Het
Asgr1 A T 11: 69,945,772 (GRCm39) D16V probably damaging Het
Camta2 A T 11: 70,573,308 (GRCm39) C227S probably benign Het
Cd22 A T 7: 30,572,658 (GRCm39) L317Q probably damaging Het
Cenpf A T 1: 189,386,112 (GRCm39) I2056K probably benign Het
Cenpi T A X: 133,218,782 (GRCm39) F161L possibly damaging Het
Ciapin1 C T 8: 95,559,161 (GRCm39) V43I probably benign Het
Cox5b-ps T G 13: 21,685,294 (GRCm39) T99P possibly damaging Het
Dach1 A G 14: 98,068,777 (GRCm39) L601P probably damaging Het
Ddx11 T A 17: 66,455,734 (GRCm39) L711Q probably damaging Het
Dsg1a A T 18: 20,471,707 (GRCm39) N653I probably damaging Het
Fezf2 G T 14: 12,344,405 (GRCm38) P261T probably benign Het
Galnt5 T C 2: 57,914,735 (GRCm39) probably null Het
Gemin5 A G 11: 58,027,743 (GRCm39) L935P probably damaging Het
Gm9507 A T 10: 77,647,519 (GRCm39) C53* probably null Het
Irgm2 A G 11: 58,110,902 (GRCm39) I198V probably damaging Het
Kcnh8 GAGACCAACGAGCAGCTGATGCTTCAGA GAGA 17: 53,032,934 (GRCm39) 74 probably benign Het
Klf12 A C 14: 100,387,162 (GRCm39) probably null Het
Lpp C T 16: 24,480,451 (GRCm39) P73L probably damaging Het
Lrrc34 G A 3: 30,696,890 (GRCm39) H127Y probably benign Het
Lyar T C 5: 38,382,053 (GRCm39) S12P probably damaging Het
Maml3 A G 3: 52,011,473 (GRCm39) I31T unknown Het
Mei1 A G 15: 81,987,513 (GRCm39) N859S probably benign Het
Minar2 T A 18: 59,208,739 (GRCm39) M129K probably damaging Het
Mysm1 A T 4: 94,840,450 (GRCm39) N655K probably benign Het
Npnt T C 3: 132,653,893 (GRCm39) I29M probably benign Het
Nrxn1 A G 17: 91,395,746 (GRCm39) W137R probably benign Het
Numb C T 12: 83,844,118 (GRCm39) probably null Het
Obsl1 A G 1: 75,482,480 (GRCm39) F130S probably damaging Het
Or2h1 T G 17: 37,404,295 (GRCm39) Q157P probably damaging Het
Or4k47 T A 2: 111,451,586 (GRCm39) I278F probably benign Het
Pbx4 T C 8: 70,322,776 (GRCm39) V294A probably benign Het
Pde4dip A C 3: 97,664,312 (GRCm39) L524R possibly damaging Het
Plppr1 G A 4: 49,337,655 (GRCm39) A319T probably benign Het
Ppid A T 3: 79,500,925 (GRCm39) I32F probably damaging Het
Ppp4r3c1 A T X: 88,975,051 (GRCm39) V382E probably damaging Het
Prkcsh A G 9: 21,924,164 (GRCm39) D458G probably damaging Het
Prr27 A G 5: 87,991,261 (GRCm39) E291G probably benign Het
Psme4 A T 11: 30,782,615 (GRCm39) K923N possibly damaging Het
Rab25 T C 3: 88,450,765 (GRCm39) T45A probably damaging Het
Rapgef1 C T 2: 29,612,239 (GRCm39) P630S probably benign Het
Rasa2 T C 9: 96,452,821 (GRCm39) D355G probably damaging Het
Rel C T 11: 23,692,761 (GRCm39) G424D probably benign Het
Rtn4 A T 11: 29,658,634 (GRCm39) E929D probably benign Het
Samt3 A C X: 85,090,740 (GRCm39) M211L probably benign Het
Slk T G 19: 47,600,428 (GRCm39) I151S probably damaging Het
Spin1 T A 13: 51,298,506 (GRCm39) V175D probably damaging Het
Ssxb10 A G X: 8,197,258 (GRCm39) D77G probably benign Het
Ticam1 C T 17: 56,578,555 (GRCm39) R180H probably damaging Het
Tmem151b G C 17: 45,856,387 (GRCm39) P351R possibly damaging Het
Tmod1 A C 4: 46,061,043 (GRCm39) Y10S probably damaging Het
Tns2 C T 15: 102,017,369 (GRCm39) R281C probably damaging Het
Trpm1 T C 7: 63,858,182 (GRCm39) Y225H possibly damaging Het
Ttc17 T C 2: 94,157,049 (GRCm39) N411S probably benign Het
Tyro3 A G 2: 119,639,298 (GRCm39) D335G probably benign Het
Unc79 C A 12: 102,977,538 (GRCm39) Y180* probably null Het
Upp1 A T 11: 9,084,872 (GRCm39) D197V possibly damaging Het
Vmn1r226 T A 17: 20,908,308 (GRCm39) M180K possibly damaging Het
Vtn T A 11: 78,392,724 (GRCm39) I434N probably damaging Het
Zfp819 A G 7: 43,265,885 (GRCm39) T47A probably benign Het
Zyg11b G A 4: 108,123,127 (GRCm39) T280I probably damaging Het
Other mutations in Zfp329
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02501:Zfp329 APN 7 12,545,106 (GRCm39) missense possibly damaging 0.87
IGL02830:Zfp329 APN 7 12,544,043 (GRCm39) missense probably damaging 1.00
R0069:Zfp329 UTSW 7 12,544,859 (GRCm39) missense probably damaging 0.98
R0069:Zfp329 UTSW 7 12,544,859 (GRCm39) missense probably damaging 0.98
R0122:Zfp329 UTSW 7 12,544,914 (GRCm39) missense probably damaging 1.00
R0238:Zfp329 UTSW 7 12,544,756 (GRCm39) missense probably damaging 1.00
R0238:Zfp329 UTSW 7 12,544,756 (GRCm39) missense probably damaging 1.00
R0539:Zfp329 UTSW 7 12,540,520 (GRCm39) critical splice acceptor site probably null
R0570:Zfp329 UTSW 7 12,544,379 (GRCm39) missense probably damaging 1.00
R0682:Zfp329 UTSW 7 12,544,211 (GRCm39) missense probably damaging 1.00
R0811:Zfp329 UTSW 7 12,545,395 (GRCm39) missense probably benign
R0812:Zfp329 UTSW 7 12,545,395 (GRCm39) missense probably benign
R0944:Zfp329 UTSW 7 12,545,395 (GRCm39) missense probably benign
R0945:Zfp329 UTSW 7 12,545,395 (GRCm39) missense probably benign
R0946:Zfp329 UTSW 7 12,545,395 (GRCm39) missense probably benign
R0948:Zfp329 UTSW 7 12,545,395 (GRCm39) missense probably benign
R1632:Zfp329 UTSW 7 12,544,876 (GRCm39) missense possibly damaging 0.63
R2172:Zfp329 UTSW 7 12,544,694 (GRCm39) missense probably damaging 1.00
R2897:Zfp329 UTSW 7 12,544,413 (GRCm39) missense probably damaging 1.00
R4256:Zfp329 UTSW 7 12,541,840 (GRCm39) missense probably benign 0.03
R4383:Zfp329 UTSW 7 12,545,584 (GRCm39) start gained probably benign
R4384:Zfp329 UTSW 7 12,545,584 (GRCm39) start gained probably benign
R4692:Zfp329 UTSW 7 12,544,559 (GRCm39) missense probably damaging 1.00
R5260:Zfp329 UTSW 7 12,540,453 (GRCm39) unclassified probably benign
R5327:Zfp329 UTSW 7 12,545,421 (GRCm39) missense probably benign 0.04
R5679:Zfp329 UTSW 7 12,543,958 (GRCm39) missense probably damaging 0.96
R6886:Zfp329 UTSW 7 12,544,025 (GRCm39) missense probably benign 0.00
R6904:Zfp329 UTSW 7 12,540,457 (GRCm39) unclassified probably benign
R7304:Zfp329 UTSW 7 12,544,826 (GRCm39) missense probably damaging 1.00
R7564:Zfp329 UTSW 7 12,544,967 (GRCm39) missense probably damaging 1.00
R8130:Zfp329 UTSW 7 12,544,313 (GRCm39) missense probably damaging 1.00
R8310:Zfp329 UTSW 7 12,544,116 (GRCm39) nonsense probably null
R8788:Zfp329 UTSW 7 12,544,490 (GRCm39) missense possibly damaging 0.85
R9206:Zfp329 UTSW 7 12,545,085 (GRCm39) missense probably benign
R9497:Zfp329 UTSW 7 12,544,215 (GRCm39) nonsense probably null
R9656:Zfp329 UTSW 7 12,544,417 (GRCm39) missense probably damaging 1.00
R9707:Zfp329 UTSW 7 12,544,129 (GRCm39) missense probably benign 0.01
Predicted Primers PCR Primer
(F):5'- ACAAGTTGAGTGGCTTCCATG -3'
(R):5'- GCTCAGAATGTTCCTGAAGAAG -3'

Sequencing Primer
(F):5'- ATCGCCGTCAGCAGTTC -3'
(R):5'- TGTTCCTGAAGAAGAAAGTATTTCC -3'
Posted On 2014-08-25