Incidental Mutation 'R2035:2210408I21Rik'
ID 224527
Institutional Source Beutler Lab
Gene Symbol 2210408I21Rik
Ensembl Gene ENSMUSG00000071252
Gene Name RIKEN cDNA 2210408I21 gene
Synonyms
MMRRC Submission 040042-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R2035 (G1)
Quality Score 225
Status Not validated
Chromosome 13
Chromosomal Location 77283659-77761903 bp(+) (GRCm39)
Type of Mutation makesense
DNA Base Change (assembly) T to C at 77760761 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Stop codon to Glutamine at position 1273 (*1273Q)
Ref Sequence ENSEMBL: ENSMUSP00000127449 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000168779]
AlphaFold no structure available at present
Predicted Effect probably null
Transcript: ENSMUST00000168779
AA Change: *1273Q
SMART Domains Protein: ENSMUSP00000127449
Gene: ENSMUSG00000071252
AA Change: *1273Q

DomainStartEndE-ValueType
low complexity region 121 133 N/A INTRINSIC
low complexity region 151 164 N/A INTRINSIC
Pfam:DUF4495 515 832 1.6e-140 PFAM
low complexity region 1241 1255 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 93.9%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aars2 A G 17: 45,825,727 (GRCm39) I348V possibly damaging Het
Abca8b G A 11: 109,847,932 (GRCm39) R788C possibly damaging Het
Abhd15 T C 11: 77,406,536 (GRCm39) L171P probably damaging Het
Abi3bp A T 16: 56,480,581 (GRCm39) H686L probably benign Het
Acsl1 A G 8: 46,981,621 (GRCm39) Y456C probably damaging Het
Arsi G A 18: 61,049,723 (GRCm39) G202E probably benign Het
Ash1l T A 3: 88,973,624 (GRCm39) V2561D probably benign Het
B3galnt2 T A 13: 14,140,909 (GRCm39) F44I probably benign Het
Bicra A T 7: 15,730,338 (GRCm39) H24Q possibly damaging Het
Ccdc163 T C 4: 116,568,530 (GRCm39) S195P probably damaging Het
Cd163 A T 6: 124,297,588 (GRCm39) K911N probably damaging Het
Clcn3 A T 8: 61,387,632 (GRCm39) S179T probably damaging Het
Dctn4 T A 18: 60,671,489 (GRCm39) D120E possibly damaging Het
Dnali1 C T 4: 124,952,903 (GRCm39) V207M probably damaging Het
Dnhd1 A G 7: 105,354,128 (GRCm39) Q3036R probably damaging Het
Dst G A 1: 34,310,494 (GRCm39) R4098H probably damaging Het
Eml5 A T 12: 98,760,525 (GRCm39) N1741K probably benign Het
Enah G A 1: 181,749,537 (GRCm39) P415L probably damaging Het
Entr1 T A 2: 26,273,639 (GRCm39) S374C probably damaging Het
Ess2 A G 16: 17,727,950 (GRCm39) probably null Het
F8 ATCTCTCTC ATCTCTC X: 74,366,604 (GRCm39) probably null Het
Gm4841 T G 18: 60,402,929 (GRCm39) Y388S probably benign Het
Grin3a T C 4: 49,771,336 (GRCm39) T479A probably damaging Het
Gucy2e C A 11: 69,118,358 (GRCm39) V743L probably benign Het
Il33 T A 19: 29,932,037 (GRCm39) N143K probably damaging Het
Ism1 T A 2: 139,599,075 (GRCm39) S349R probably damaging Het
Itgb2 T A 10: 77,383,033 (GRCm39) D134E probably damaging Het
Kcnk1 A C 8: 126,752,108 (GRCm39) N238T possibly damaging Het
Kcnu1 G T 8: 26,386,721 (GRCm39) V535L probably benign Het
Muc19 T A 15: 91,776,599 (GRCm39) noncoding transcript Het
Mycbp2 A T 14: 103,497,675 (GRCm39) Y966N probably damaging Het
Myo19 A T 11: 84,788,434 (GRCm39) M349L probably benign Het
Narf G A 11: 121,129,326 (GRCm39) A37T probably benign Het
Ncapd2 T C 6: 125,161,491 (GRCm39) N208D probably benign Het
Nr1i2 A G 16: 38,071,488 (GRCm39) probably null Het
Opn5 A G 17: 42,918,052 (GRCm39) I70T probably damaging Het
Or52e19 T A 7: 102,959,463 (GRCm39) H178Q probably damaging Het
Or7a38 T C 10: 78,753,421 (GRCm39) V249A possibly damaging Het
Pkn2 G T 3: 142,526,348 (GRCm39) P410T probably damaging Het
Pla2r1 T C 2: 60,253,080 (GRCm39) N1337S probably damaging Het
Prkd3 A T 17: 79,282,802 (GRCm39) probably null Het
Pum2 T A 12: 8,778,638 (GRCm39) Y429* probably null Het
Resf1 G A 6: 149,230,724 (GRCm39) V1257I possibly damaging Het
Rttn T A 18: 89,038,340 (GRCm39) V812E probably damaging Het
Rufy2 T A 10: 62,842,526 (GRCm39) L483H probably damaging Het
Slc35a3 T A 3: 116,480,972 (GRCm39) Q97L probably damaging Het
St18 A T 1: 6,872,552 (GRCm39) M96L probably benign Het
Strc G A 2: 121,205,415 (GRCm39) A905V probably damaging Het
Syne3 T C 12: 104,924,386 (GRCm39) M338V probably benign Het
Syngr2 A G 11: 117,704,186 (GRCm39) D187G probably benign Het
Tas2r109 A C 6: 132,957,423 (GRCm39) I169R probably benign Het
Tbc1d22a T A 15: 86,275,266 (GRCm39) probably null Het
Thbs1 T A 2: 117,948,821 (GRCm39) probably null Het
Them6 C A 15: 74,593,524 (GRCm39) D127E probably damaging Het
Tmem132d T C 5: 127,869,522 (GRCm39) D604G probably damaging Het
Tnni1 A G 1: 135,733,330 (GRCm39) T51A probably benign Het
Topors T C 4: 40,262,879 (GRCm39) N135S probably damaging Het
Unc80 A T 1: 66,645,752 (GRCm39) D1476V probably damaging Het
Vmn1r17 A G 6: 57,337,573 (GRCm39) V264A probably benign Het
Vmn1r193 T A 13: 22,403,732 (GRCm39) T87S probably benign Het
Vmn1r202 C A 13: 22,685,772 (GRCm39) R215L probably damaging Het
Vmn2r24 A T 6: 123,793,019 (GRCm39) N782I probably damaging Het
Vmn2r53 A G 7: 12,332,438 (GRCm39) F404L possibly damaging Het
Xpo4 A T 14: 57,823,383 (GRCm39) C1036S possibly damaging Het
Yae1d1 T C 13: 18,164,306 (GRCm39) N104D probably benign Het
Zan C A 5: 137,442,209 (GRCm39) R1901L unknown Het
Zbtb9 G A 17: 27,193,897 (GRCm39) R434H probably damaging Het
Zdhhc23 A C 16: 43,793,871 (GRCm39) C268G probably damaging Het
Other mutations in 2210408I21Rik
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00164:2210408I21Rik APN 13 77,471,477 (GRCm39) splice site probably benign
IGL01154:2210408I21Rik APN 13 77,429,213 (GRCm39) missense probably benign 0.01
IGL01461:2210408I21Rik APN 13 77,429,214 (GRCm39) missense probably benign 0.25
IGL01624:2210408I21Rik APN 13 77,341,205 (GRCm39) missense probably damaging 0.99
IGL02033:2210408I21Rik APN 13 77,407,995 (GRCm39) missense possibly damaging 0.90
IGL02621:2210408I21Rik APN 13 77,408,150 (GRCm39) missense possibly damaging 0.92
IGL02718:2210408I21Rik APN 13 77,322,991 (GRCm39) missense probably damaging 1.00
IGL02823:2210408I21Rik APN 13 77,410,074 (GRCm39) missense probably damaging 0.96
IGL02859:2210408I21Rik APN 13 77,415,818 (GRCm39) missense possibly damaging 0.71
IGL03006:2210408I21Rik APN 13 77,471,891 (GRCm39) critical splice donor site probably null
IGL03072:2210408I21Rik APN 13 77,408,116 (GRCm39) missense probably benign
IGL03184:2210408I21Rik APN 13 77,471,570 (GRCm39) missense possibly damaging 0.63
IGL03275:2210408I21Rik APN 13 77,446,674 (GRCm39) missense possibly damaging 0.71
PIT4651001:2210408I21Rik UTSW 13 77,408,014 (GRCm39) missense probably benign
R0226:2210408I21Rik UTSW 13 77,451,544 (GRCm39) missense possibly damaging 0.86
R0323:2210408I21Rik UTSW 13 77,446,674 (GRCm39) missense possibly damaging 0.71
R0614:2210408I21Rik UTSW 13 77,340,782 (GRCm39) missense probably benign 0.26
R0894:2210408I21Rik UTSW 13 77,471,726 (GRCm39) missense probably benign 0.18
R1165:2210408I21Rik UTSW 13 77,482,406 (GRCm39) missense probably benign 0.06
R1509:2210408I21Rik UTSW 13 77,340,766 (GRCm39) missense probably benign
R1711:2210408I21Rik UTSW 13 77,418,039 (GRCm39) missense possibly damaging 0.93
R1714:2210408I21Rik UTSW 13 77,464,479 (GRCm39) missense possibly damaging 0.86
R1718:2210408I21Rik UTSW 13 77,393,489 (GRCm39) intron probably benign
R1836:2210408I21Rik UTSW 13 77,471,493 (GRCm39) missense probably benign 0.00
R1893:2210408I21Rik UTSW 13 77,415,928 (GRCm39) missense possibly damaging 0.93
R2329:2210408I21Rik UTSW 13 77,451,444 (GRCm39) missense probably benign 0.04
R2897:2210408I21Rik UTSW 13 77,471,640 (GRCm39) missense probably benign 0.33
R3688:2210408I21Rik UTSW 13 77,415,968 (GRCm39) missense possibly damaging 0.52
R4153:2210408I21Rik UTSW 13 77,341,292 (GRCm39) missense probably benign 0.00
R4387:2210408I21Rik UTSW 13 77,464,693 (GRCm39) critical splice donor site probably null
R4388:2210408I21Rik UTSW 13 77,464,693 (GRCm39) critical splice donor site probably null
R4499:2210408I21Rik UTSW 13 77,464,646 (GRCm39) missense possibly damaging 0.96
R4614:2210408I21Rik UTSW 13 77,402,375 (GRCm39) splice site probably null
R4798:2210408I21Rik UTSW 13 77,471,843 (GRCm39) missense possibly damaging 0.96
R4943:2210408I21Rik UTSW 13 77,393,446 (GRCm39) missense possibly damaging 0.86
R5045:2210408I21Rik UTSW 13 77,415,927 (GRCm39) splice site probably null
R5387:2210408I21Rik UTSW 13 77,408,092 (GRCm39) missense probably benign 0.11
R5500:2210408I21Rik UTSW 13 77,451,508 (GRCm39) missense probably benign 0.33
R5686:2210408I21Rik UTSW 13 77,451,433 (GRCm39) missense possibly damaging 0.72
R6111:2210408I21Rik UTSW 13 77,476,021 (GRCm39) missense possibly damaging 0.72
R6135:2210408I21Rik UTSW 13 77,402,335 (GRCm39) missense probably damaging 0.98
R6188:2210408I21Rik UTSW 13 77,331,850 (GRCm39) missense possibly damaging 0.53
R6388:2210408I21Rik UTSW 13 77,410,230 (GRCm39) missense probably benign
R6588:2210408I21Rik UTSW 13 77,340,766 (GRCm39) missense probably benign
R6632:2210408I21Rik UTSW 13 77,429,186 (GRCm39) missense possibly damaging 0.86
R6638:2210408I21Rik UTSW 13 77,451,521 (GRCm39) missense probably benign 0.07
R6755:2210408I21Rik UTSW 13 77,475,994 (GRCm39) missense probably benign
R6971:2210408I21Rik UTSW 13 77,341,306 (GRCm39) missense possibly damaging 0.90
R7079:2210408I21Rik UTSW 13 77,402,323 (GRCm39) missense possibly damaging 0.86
R7130:2210408I21Rik UTSW 13 77,418,021 (GRCm39) missense possibly damaging 0.93
R7215:2210408I21Rik UTSW 13 77,471,690 (GRCm39) missense possibly damaging 0.96
R7272:2210408I21Rik UTSW 13 77,471,655 (GRCm39) missense probably benign 0.00
R7331:2210408I21Rik UTSW 13 77,331,728 (GRCm39) missense possibly damaging 0.53
R7561:2210408I21Rik UTSW 13 77,341,314 (GRCm39) missense probably benign
R7684:2210408I21Rik UTSW 13 77,760,659 (GRCm39) nonsense probably null
R7728:2210408I21Rik UTSW 13 77,464,596 (GRCm39) missense possibly damaging 0.96
R7881:2210408I21Rik UTSW 13 77,471,685 (GRCm39) missense possibly damaging 0.53
R7963:2210408I21Rik UTSW 13 77,340,673 (GRCm39) missense probably benign 0.02
R8008:2210408I21Rik UTSW 13 77,429,234 (GRCm39) missense probably benign 0.28
R8024:2210408I21Rik UTSW 13 77,760,713 (GRCm39) missense probably benign
R8170:2210408I21Rik UTSW 13 77,411,713 (GRCm39) missense probably benign 0.06
R8201:2210408I21Rik UTSW 13 77,341,278 (GRCm39) missense possibly damaging 0.72
R8255:2210408I21Rik UTSW 13 77,415,850 (GRCm39) missense possibly damaging 0.71
R8296:2210408I21Rik UTSW 13 77,415,896 (GRCm39) missense probably damaging 0.98
R8476:2210408I21Rik UTSW 13 77,410,020 (GRCm39) missense possibly damaging 0.92
R8526:2210408I21Rik UTSW 13 77,417,935 (GRCm39) nonsense probably null
R8746:2210408I21Rik UTSW 13 77,451,529 (GRCm39) missense probably benign 0.01
R8812:2210408I21Rik UTSW 13 77,480,471 (GRCm39) missense probably damaging 0.98
R8870:2210408I21Rik UTSW 13 77,471,840 (GRCm39) missense possibly damaging 0.96
R8885:2210408I21Rik UTSW 13 77,471,525 (GRCm39) missense possibly damaging 0.91
R8910:2210408I21Rik UTSW 13 77,471,768 (GRCm39) missense probably benign 0.03
R8911:2210408I21Rik UTSW 13 77,429,234 (GRCm39) missense probably benign 0.28
R8965:2210408I21Rik UTSW 13 77,760,723 (GRCm39) missense probably benign 0.02
R8968:2210408I21Rik UTSW 13 77,480,429 (GRCm39) nonsense probably null
R8989:2210408I21Rik UTSW 13 77,760,724 (GRCm39) missense probably benign 0.01
R9163:2210408I21Rik UTSW 13 77,393,400 (GRCm39) missense possibly damaging 0.73
R9378:2210408I21Rik UTSW 13 77,471,735 (GRCm39) missense possibly damaging 0.53
R9478:2210408I21Rik UTSW 13 77,451,573 (GRCm39) missense possibly damaging 0.53
R9523:2210408I21Rik UTSW 13 77,407,988 (GRCm39) missense possibly damaging 0.53
R9595:2210408I21Rik UTSW 13 77,464,566 (GRCm39) missense probably benign
X0066:2210408I21Rik UTSW 13 77,331,759 (GRCm39) missense possibly damaging 0.72
Z1088:2210408I21Rik UTSW 13 77,323,010 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGAATAGGAAAGCTGAACCCTC -3'
(R):5'- ACACGAGATGGCAGCAATTTTATC -3'

Sequencing Primer
(F):5'- CCTCAATAAACCAGTGACTGTATATC -3'
(R):5'- AGAGACTATTGGGTGCAC -3'
Posted On 2014-08-25