Mutagenetix > Incidental Mutation

Incidental Mutation 'R2075:Tnc'

227504

Institutional Source

Beutler Lab

Gene Symbol

Tnc

Ensembl Gene

ENSMUSG00000028364

Gene Name

tenascin C

Synonyms

cytotactin, C130033P17Rik, TN-C, hexabrachion, tenascin-C, Hxb, TN

MMRRC Submission

040080-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2075 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

63878022-63965252 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 63913903 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 1303 (T1303M)

Ref Sequence

ENSEMBL: ENSMUSP00000103000 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030056] [ENSMUST00000107372] [ENSMUST00000107377]

AlphaFold

Q80YX1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000030056
AA Change: T1303M

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000030056
Gene: ENSMUSG00000028364
AA Change: T1303M

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107372
AA Change: T1303M

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000102995
Gene: ENSMUSG00000028364
AA Change: T1303M

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	2.75e0	SMART
FN3	1529	1608	3.4e-4	SMART
FN3	1619	1697	1.55e-7	SMART
FN3	1708	1785	1.53e-6	SMART
FN3	1796	1873	7.75e-8	SMART
FBG	1888	2098	4.08e-124	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107377
AA Change: T1303M

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000103000
Gene: ENSMUSG00000028364
AA Change: T1303M

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135042

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141428

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
PHENOTYPE: Mice homozygous for several different targeted mutations show variable behavioral and nervous system phenotypes such as abnormal circadian rhythm, anxiety behavior, novelty-induced activity, swimming, impaired synaptic plasticity, long term potentiation and serotonin and dopamine neurotransmission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	C	11: 9,472,382 (GRCm39)	F4263L	probably damaging	Het
Aebp2	G	A	6: 140,579,420 (GRCm39)	S219N	probably benign	Het
Anln	C	A	9: 22,244,464 (GRCm39)	W1083L	probably benign	Het
Atic	T	A	1: 71,615,286 (GRCm39)	D438E	probably benign	Het
Bahcc1	G	A	11: 120,162,515 (GRCm39)	C271Y	probably damaging	Het
Btbd17	A	T	11: 114,682,778 (GRCm39)		probably null	Het
Ccdc122	T	C	14: 77,306,391 (GRCm39)		probably null	Het
Ccdc149	A	G	5: 52,596,510 (GRCm39)	L34P	probably damaging	Het
Cd22	C	G	7: 30,569,123 (GRCm39)	C637S	probably damaging	Het
Ces1a	T	A	8: 93,774,703 (GRCm39)	N12Y	probably benign	Het
Chmp1a	A	T	8: 123,934,761 (GRCm39)	M65K	probably damaging	Het
Clec16a	G	A	16: 10,559,480 (GRCm39)	A918T	probably benign	Het
Clec2m	C	T	6: 129,303,666 (GRCm39)	E100K	probably benign	Het
Cnot1	G	T	8: 96,466,461 (GRCm39)	T1592K	possibly damaging	Het
Ddx10	A	T	9: 53,151,805 (GRCm39)	D73E	probably benign	Het
Dhx32	T	C	7: 133,323,021 (GRCm39)	N731S	probably benign	Het
Dmd	G	C	X: 83,356,089 (GRCm39)	A2257P	probably benign	Het
Dna2	A	G	10: 62,805,601 (GRCm39)	Q946R	probably benign	Het
Dnai4	G	A	4: 102,907,390 (GRCm39)	T632M	probably damaging	Het
Duox2	T	A	2: 122,125,639 (GRCm39)	S323C	probably damaging	Het
Esr2	A	G	12: 76,212,221 (GRCm39)		probably null	Het
Fbxo9	G	T	9: 77,991,798 (GRCm39)	H397N	possibly damaging	Het
Fsip2	T	A	2: 82,818,923 (GRCm39)	N4885K	possibly damaging	Het
Gm12695	T	C	4: 96,612,182 (GRCm39)	Y527C	possibly damaging	Het
Hivep1	A	G	13: 42,309,794 (GRCm39)	D678G	probably damaging	Het
Hmcn1	T	A	1: 150,453,074 (GRCm39)	I5414F	possibly damaging	Het
Hspb2	T	C	9: 50,662,646 (GRCm39)	Y67C	probably benign	Het
Kbtbd8	T	A	6: 95,103,664 (GRCm39)	C438S	possibly damaging	Het
Kif5a	T	C	10: 127,081,238 (GRCm39)	D232G	probably damaging	Het
Ky	A	G	9: 102,419,945 (GRCm39)	S651G	probably damaging	Het
Lgals12	T	C	19: 7,576,210 (GRCm39)	D238G	possibly damaging	Het
Lpcat3	T	A	6: 124,680,066 (GRCm39)	Y380N	probably damaging	Het
Mdn1	A	G	4: 32,716,058 (GRCm39)	H2080R	probably benign	Het
Mex3c	C	T	18: 73,722,840 (GRCm39)	S311L	probably benign	Het
Mlh3	A	T	12: 85,315,915 (GRCm39)	Y90*	probably null	Het
Mpped2	T	A	2: 106,575,147 (GRCm39)	Y77*	probably null	Het
Myo5a	A	T	9: 75,097,200 (GRCm39)	T49S	probably benign	Het
Nsd1	T	C	13: 55,458,313 (GRCm39)	V2142A	possibly damaging	Het
Or4a74	A	G	2: 89,439,822 (GRCm39)	V208A	probably benign	Het
Or51b17	T	A	7: 103,542,127 (GRCm39)	I272F	probably damaging	Het
Or51i2	T	A	7: 103,689,180 (GRCm39)	M59K	probably damaging	Het
Or5ac19	T	C	16: 59,089,274 (GRCm39)	Y252C	possibly damaging	Het
Pdgfra	T	C	5: 75,348,609 (GRCm39)	I883T	probably damaging	Het
Pdzd2	A	G	15: 12,385,905 (GRCm39)	L955P	probably damaging	Het
Phf24	A	G	4: 42,939,507 (GRCm39)	Y333C	possibly damaging	Het
Phlpp2	T	C	8: 110,655,124 (GRCm39)	S605P	possibly damaging	Het
Piezo2	C	T	18: 63,214,805 (GRCm39)	E1263K	probably damaging	Het
Pitrm1	A	G	13: 6,605,419 (GRCm39)	T149A	probably damaging	Het
Pkhd1l1	G	T	15: 44,422,035 (GRCm39)	A3102S	probably damaging	Het
Plch2	A	T	4: 155,074,366 (GRCm39)	L754Q	probably damaging	Het
Polr2c	A	G	8: 95,590,195 (GRCm39)	I267V	probably benign	Het
Potefam1	T	C	2: 111,030,763 (GRCm39)	E382G	probably damaging	Het
Prss8	C	A	7: 127,526,266 (GRCm39)	R148L	possibly damaging	Het
Ptpn2	T	A	18: 67,814,545 (GRCm39)	T155S	probably damaging	Het
Ptprn2	G	A	12: 117,211,337 (GRCm39)	V839I	probably benign	Het
Sbp	A	G	17: 24,164,132 (GRCm39)		probably null	Het
Sec16a	A	G	2: 26,330,251 (GRCm39)	I588T	probably damaging	Het
Six2	A	G	17: 85,994,933 (GRCm39)	S150P	probably damaging	Het
Slc5a5	C	T	8: 71,345,083 (GRCm39)	G75R	possibly damaging	Het
Slc6a20b	T	A	9: 123,424,099 (GRCm39)	T623S	probably benign	Het
Sqle	T	A	15: 59,195,750 (GRCm39)	V342E	probably damaging	Het
Stk3	T	C	15: 34,959,195 (GRCm39)	M256V	possibly damaging	Het
Tek	A	G	4: 94,715,966 (GRCm39)	I463V	probably benign	Het
Tex10	C	T	4: 48,456,800 (GRCm39)	R637Q	probably benign	Het
Traf6	A	C	2: 101,527,398 (GRCm39)	I383L	probably benign	Het
Tubb3	C	T	8: 124,148,009 (GRCm39)	A314V	probably damaging	Het
Vmn1r199	A	T	13: 22,567,435 (GRCm39)	Y243F	probably damaging	Het
Vmn2r111	A	C	17: 22,778,043 (GRCm39)	N545K	probably damaging	Het
Vmn2r27	T	A	6: 124,177,510 (GRCm39)	Q498L	possibly damaging	Het
Vstm4	A	T	14: 32,639,811 (GRCm39)	S229C	probably damaging	Het
Wdr12	C	T	1: 60,130,222 (GRCm39)	R63Q	possibly damaging	Het
Wrn	T	A	8: 33,812,357 (GRCm39)	I187L	probably benign	Het
Xirp2	A	T	2: 67,340,545 (GRCm39)	I929L	probably benign	Het
Ywhae	T	A	11: 75,655,486 (GRCm39)	D252E	probably benign	Het
Zfp944	A	T	17: 22,558,178 (GRCm39)	C356*	probably null	Het

Other mutations in Tnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Tnc	APN	4	63,935,061 (GRCm39)	splice site	probably benign
IGL00531:Tnc	APN	4	63,889,390 (GRCm39)	splice site	probably benign
IGL00674:Tnc	APN	4	63,883,844 (GRCm39)	missense	probably damaging	1.00
IGL01015:Tnc	APN	4	63,935,571 (GRCm39)	missense	probably benign	0.19
IGL01090:Tnc	APN	4	63,918,317 (GRCm39)	missense	probably damaging	1.00
IGL01310:Tnc	APN	4	63,931,314 (GRCm39)	missense	probably benign	0.03
IGL01331:Tnc	APN	4	63,901,112 (GRCm39)	missense	probably damaging	0.99
IGL01393:Tnc	APN	4	63,932,291 (GRCm39)	splice site	probably benign
IGL01411:Tnc	APN	4	63,918,959 (GRCm39)	missense	probably damaging	0.96
IGL01472:Tnc	APN	4	63,924,656 (GRCm39)	missense	probably benign	0.00
IGL01552:Tnc	APN	4	63,888,645 (GRCm39)	missense	probably damaging	1.00
IGL01661:Tnc	APN	4	63,888,544 (GRCm39)	splice site	probably benign
IGL01669:Tnc	APN	4	63,918,938 (GRCm39)	missense	probably damaging	1.00
IGL01912:Tnc	APN	4	63,926,977 (GRCm39)	missense	probably damaging	1.00
IGL02028:Tnc	APN	4	63,884,909 (GRCm39)	splice site	probably benign
IGL02100:Tnc	APN	4	63,918,398 (GRCm39)	missense	possibly damaging	0.84
IGL02549:Tnc	APN	4	63,933,309 (GRCm39)	missense	probably damaging	1.00
IGL02642:Tnc	APN	4	63,883,816 (GRCm39)	splice site	probably benign
IGL02712:Tnc	APN	4	63,893,493 (GRCm39)	missense	probably damaging	1.00
IGL02876:Tnc	APN	4	63,933,338 (GRCm39)	missense	possibly damaging	0.56
IGL02886:Tnc	APN	4	63,918,344 (GRCm39)	missense	probably damaging	0.96
IGL02972:Tnc	APN	4	63,894,715 (GRCm39)	missense	probably benign	0.11
IGL03073:Tnc	APN	4	63,889,461 (GRCm39)	missense	possibly damaging	0.58
IGL03116:Tnc	APN	4	63,932,270 (GRCm39)	missense	probably damaging	1.00
IGL03181:Tnc	APN	4	63,885,543 (GRCm39)	missense	possibly damaging	0.95
IGL03358:Tnc	APN	4	63,935,852 (GRCm39)	nonsense	probably null
tancredo	UTSW	4	63,911,534 (GRCm39)	nonsense	probably null
BB009:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
BB019:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
P0020:Tnc	UTSW	4	63,927,094 (GRCm39)	missense	possibly damaging	0.63
PIT4377001:Tnc	UTSW	4	63,935,973 (GRCm39)	missense	probably damaging	1.00
PIT4403001:Tnc	UTSW	4	63,882,904 (GRCm39)	missense	probably damaging	1.00
PIT4468001:Tnc	UTSW	4	63,882,904 (GRCm39)	missense	probably damaging	1.00
R0243:Tnc	UTSW	4	63,888,657 (GRCm39)	missense	probably damaging	0.98
R0362:Tnc	UTSW	4	63,935,679 (GRCm39)	missense	probably damaging	1.00
R0410:Tnc	UTSW	4	63,925,931 (GRCm39)	missense	probably benign	0.00
R0420:Tnc	UTSW	4	63,918,396 (GRCm39)	missense	probably benign	0.00
R0540:Tnc	UTSW	4	63,938,692 (GRCm39)	missense	probably damaging	1.00
R0650:Tnc	UTSW	4	63,926,971 (GRCm39)	missense	probably benign	0.00
R1019:Tnc	UTSW	4	63,880,319 (GRCm39)	missense	probably damaging	1.00
R1102:Tnc	UTSW	4	63,938,705 (GRCm39)	missense	probably benign	0.05
R1126:Tnc	UTSW	4	63,936,357 (GRCm39)	missense	probably damaging	0.99
R1141:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1142:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1307:Tnc	UTSW	4	63,927,096 (GRCm39)	missense	probably damaging	0.98
R1322:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1414:Tnc	UTSW	4	63,883,932 (GRCm39)	splice site	probably benign
R1470:Tnc	UTSW	4	63,884,811 (GRCm39)	missense	probably damaging	1.00
R1470:Tnc	UTSW	4	63,884,811 (GRCm39)	missense	probably damaging	1.00
R1499:Tnc	UTSW	4	63,882,991 (GRCm39)	missense	probably benign	0.15
R1506:Tnc	UTSW	4	63,925,921 (GRCm39)	missense	possibly damaging	0.90
R1597:Tnc	UTSW	4	63,924,621 (GRCm39)	missense	probably benign
R1750:Tnc	UTSW	4	63,890,972 (GRCm39)	missense	probably damaging	1.00
R1765:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1783:Tnc	UTSW	4	63,936,333 (GRCm39)	missense	probably damaging	0.98
R1808:Tnc	UTSW	4	63,918,168 (GRCm39)	missense	probably damaging	1.00
R1903:Tnc	UTSW	4	63,918,299 (GRCm39)	missense	probably benign	0.00
R1932:Tnc	UTSW	4	63,911,262 (GRCm39)	critical splice donor site	probably null
R1941:Tnc	UTSW	4	63,933,201 (GRCm39)	missense	probably damaging	1.00
R1983:Tnc	UTSW	4	63,902,867 (GRCm39)	missense	possibly damaging	0.95
R2024:Tnc	UTSW	4	63,882,858 (GRCm39)	missense	probably damaging	1.00
R2327:Tnc	UTSW	4	63,893,475 (GRCm39)	missense	possibly damaging	0.78
R2444:Tnc	UTSW	4	63,933,200 (GRCm39)	missense	probably damaging	1.00
R2982:Tnc	UTSW	4	63,938,756 (GRCm39)	missense	possibly damaging	0.81
R3874:Tnc	UTSW	4	63,926,947 (GRCm39)	missense	probably damaging	1.00
R4110:Tnc	UTSW	4	63,933,188 (GRCm39)	missense	probably damaging	1.00
R4360:Tnc	UTSW	4	63,935,161 (GRCm39)	missense	probably benign	0.35
R4371:Tnc	UTSW	4	63,888,588 (GRCm39)	missense	probably damaging	1.00
R4434:Tnc	UTSW	4	63,926,066 (GRCm39)	missense	possibly damaging	0.91
R4438:Tnc	UTSW	4	63,926,066 (GRCm39)	missense	possibly damaging	0.91
R4570:Tnc	UTSW	4	63,913,909 (GRCm39)	missense	probably damaging	0.99
R4595:Tnc	UTSW	4	63,913,982 (GRCm39)	missense	probably damaging	1.00
R4749:Tnc	UTSW	4	63,913,876 (GRCm39)	missense	possibly damaging	0.56
R4756:Tnc	UTSW	4	63,885,580 (GRCm39)	missense	probably damaging	0.99
R4824:Tnc	UTSW	4	63,935,857 (GRCm39)	nonsense	probably null
R4957:Tnc	UTSW	4	63,894,793 (GRCm39)	missense	probably damaging	1.00
R4977:Tnc	UTSW	4	63,924,485 (GRCm39)	missense	possibly damaging	0.82
R5001:Tnc	UTSW	4	63,918,299 (GRCm39)	missense	probably benign	0.16
R5001:Tnc	UTSW	4	63,902,726 (GRCm39)	missense	probably damaging	1.00
R5015:Tnc	UTSW	4	63,924,739 (GRCm39)	missense	probably damaging	1.00
R5049:Tnc	UTSW	4	63,936,223 (GRCm39)	missense	probably damaging	1.00
R5066:Tnc	UTSW	4	63,893,466 (GRCm39)	missense	probably damaging	0.96
R5073:Tnc	UTSW	4	63,938,648 (GRCm39)	missense	probably damaging	1.00
R5116:Tnc	UTSW	4	63,885,452 (GRCm39)	critical splice donor site	probably null
R5195:Tnc	UTSW	4	63,885,489 (GRCm39)	missense	probably damaging	1.00
R5200:Tnc	UTSW	4	63,889,515 (GRCm39)	missense	probably damaging	1.00
R5221:Tnc	UTSW	4	63,911,534 (GRCm39)	nonsense	probably null
R5237:Tnc	UTSW	4	63,880,333 (GRCm39)	missense	probably damaging	1.00
R5265:Tnc	UTSW	4	63,911,443 (GRCm39)	missense	probably benign	0.00
R5275:Tnc	UTSW	4	63,882,967 (GRCm39)	nonsense	probably null
R5346:Tnc	UTSW	4	63,926,892 (GRCm39)	missense	probably benign
R5409:Tnc	UTSW	4	63,925,654 (GRCm39)	missense	probably damaging	1.00
R5409:Tnc	UTSW	4	63,884,773 (GRCm39)	missense	probably damaging	1.00
R5469:Tnc	UTSW	4	63,932,162 (GRCm39)	splice site	probably null
R5518:Tnc	UTSW	4	63,935,916 (GRCm39)	missense	probably damaging	1.00
R5560:Tnc	UTSW	4	63,926,946 (GRCm39)	missense	probably damaging	1.00
R5588:Tnc	UTSW	4	63,924,659 (GRCm39)	missense	possibly damaging	0.57
R5686:Tnc	UTSW	4	63,925,967 (GRCm39)	splice site	probably null
R5686:Tnc	UTSW	4	63,927,032 (GRCm39)	missense	possibly damaging	0.78
R5837:Tnc	UTSW	4	63,931,451 (GRCm39)	missense	probably damaging	1.00
R5976:Tnc	UTSW	4	63,936,403 (GRCm39)	missense	probably benign	0.17
R6156:Tnc	UTSW	4	63,888,589 (GRCm39)	missense	probably damaging	1.00
R6182:Tnc	UTSW	4	63,927,033 (GRCm39)	missense	probably damaging	0.99
R6360:Tnc	UTSW	4	63,918,970 (GRCm39)	missense	probably damaging	1.00
R6416:Tnc	UTSW	4	63,926,053 (GRCm39)	missense	probably benign	0.05
R6778:Tnc	UTSW	4	63,913,835 (GRCm39)	missense	probably benign	0.12
R6798:Tnc	UTSW	4	63,883,841 (GRCm39)	missense	probably benign	0.02
R6799:Tnc	UTSW	4	63,883,841 (GRCm39)	missense	probably benign	0.02
R6943:Tnc	UTSW	4	63,900,982 (GRCm39)	missense	probably damaging	0.97
R7027:Tnc	UTSW	4	63,902,826 (GRCm39)	missense	probably benign	0.02
R7183:Tnc	UTSW	4	63,931,365 (GRCm39)	missense	probably damaging	1.00
R7204:Tnc	UTSW	4	63,889,392 (GRCm39)	splice site	probably null
R7317:Tnc	UTSW	4	63,890,959 (GRCm39)	missense	probably damaging	0.99
R7323:Tnc	UTSW	4	63,889,469 (GRCm39)	missense	probably damaging	0.96
R7327:Tnc	UTSW	4	63,882,999 (GRCm39)	splice site	probably null
R7382:Tnc	UTSW	4	63,932,280 (GRCm39)	nonsense	probably null
R7399:Tnc	UTSW	4	63,938,894 (GRCm39)	start gained	probably benign
R7479:Tnc	UTSW	4	63,935,865 (GRCm39)	missense	possibly damaging	0.95
R7585:Tnc	UTSW	4	63,938,648 (GRCm39)	missense	probably damaging	1.00
R7932:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
R7947:Tnc	UTSW	4	63,935,580 (GRCm39)	missense	probably damaging	1.00
R7974:Tnc	UTSW	4	63,918,961 (GRCm39)	missense	possibly damaging	0.84
R7991:Tnc	UTSW	4	63,926,983 (GRCm39)	missense	probably benign	0.42
R8004:Tnc	UTSW	4	63,902,894 (GRCm39)	missense	probably benign	0.04
R8080:Tnc	UTSW	4	63,894,706 (GRCm39)	missense	possibly damaging	0.52
R8109:Tnc	UTSW	4	63,927,000 (GRCm39)	missense	probably benign	0.11
R8145:Tnc	UTSW	4	63,935,716 (GRCm39)	missense	probably benign
R8340:Tnc	UTSW	4	63,926,036 (GRCm39)	missense	probably damaging	1.00
R8360:Tnc	UTSW	4	63,885,511 (GRCm39)	missense	probably benign	0.00
R8671:Tnc	UTSW	4	63,935,683 (GRCm39)	missense	probably damaging	1.00
R8691:Tnc	UTSW	4	63,880,313 (GRCm39)	missense	probably damaging	1.00
R8759:Tnc	UTSW	4	63,924,501 (GRCm39)	missense	possibly damaging	0.86
R8864:Tnc	UTSW	4	63,911,296 (GRCm39)	missense	probably damaging	0.98
R8927:Tnc	UTSW	4	63,925,595 (GRCm39)	missense	probably damaging	1.00
R8928:Tnc	UTSW	4	63,925,595 (GRCm39)	missense	probably damaging	1.00
R8949:Tnc	UTSW	4	63,927,087 (GRCm39)	missense	probably damaging	1.00
R8956:Tnc	UTSW	4	63,918,970 (GRCm39)	missense	probably damaging	1.00
R9016:Tnc	UTSW	4	63,935,331 (GRCm39)	missense	probably benign	0.23
R9049:Tnc	UTSW	4	63,918,247 (GRCm39)	missense	possibly damaging	0.83
R9097:Tnc	UTSW	4	63,888,622 (GRCm39)	missense	possibly damaging	0.62
R9114:Tnc	UTSW	4	63,890,973 (GRCm39)	missense	probably benign	0.03
R9151:Tnc	UTSW	4	63,938,686 (GRCm39)	missense	possibly damaging	0.46
R9488:Tnc	UTSW	4	63,913,942 (GRCm39)	missense	probably damaging	0.99
R9537:Tnc	UTSW	4	63,884,821 (GRCm39)	missense	probably damaging	0.99
R9666:Tnc	UTSW	4	63,926,045 (GRCm39)	missense	probably damaging	1.00
R9700:Tnc	UTSW	4	63,933,186 (GRCm39)	missense	probably damaging	0.99
R9703:Tnc	UTSW	4	63,889,412 (GRCm39)	missense	probably benign	0.00
R9771:Tnc	UTSW	4	63,925,600 (GRCm39)	missense	probably damaging	1.00
S24628:Tnc	UTSW	4	63,936,249 (GRCm39)	missense	probably damaging	1.00
Z1177:Tnc	UTSW	4	63,925,663 (GRCm39)	nonsense	probably null
Z1177:Tnc	UTSW	4	63,878,781 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- AGAAAAGCTAGTTCCCTTGCC -3'
(R):5'- AACAGCAAATTCTCTGCCTTC -3'

Sequencing Primer
(F):5'- GTTCCCTTGCCATCTCAGAGAGAG -3'
(R):5'- AACAGCAAATTCTCTGCCTTCTTCAG -3'

Posted On

2014-09-17