Mutagenetix > Incidental Mutation

Incidental Mutation 'R2127:Atp2b2'

227610

Institutional Source

Beutler Lab

Gene Symbol

Atp2b2

Ensembl Gene

ENSMUSG00000030302

Gene Name

ATPase, Ca++ transporting, plasma membrane 2

Synonyms

PMCA2, Gena300, wms, D6Abb2e, jog, Tmy

MMRRC Submission

040130-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.889) question?

Stock #

R2127 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

113720803-114019574 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 113737611 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 921 (L921P)

Ref Sequence

ENSEMBL: ENSMUSP00000145174 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089003] [ENSMUST00000101044] [ENSMUST00000101045] [ENSMUST00000152831] [ENSMUST00000205052]

AlphaFold

Q9R0K7

Predicted Effect

probably damaging
Transcript: ENSMUST00000089003
AA Change: L925P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000086398
Gene: ENSMUSG00000030302
AA Change: L925P

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	156	444	1.7e-56	PFAM
Pfam:Hydrolase	448	787	3.9e-25	PFAM
Pfam:HAD	451	784	2.4e-16	PFAM
Pfam:Hydrolase_like2	497	593	9.4e-17	PFAM
Pfam:Hydrolase_3	745	820	1.7e-6	PFAM
transmembrane domain	833	855	N/A	INTRINSIC
Pfam:Cation_ATPase_C	857	1039	7.3e-46	PFAM
low complexity region	1057	1070	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1081	1144	1.4e-31	PFAM
low complexity region	1151	1166	N/A	INTRINSIC
low complexity region	1175	1189	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101044
AA Change: L970P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000098605
Gene: ENSMUSG00000030302
AA Change: L970P

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	155	307	4.2e-28	PFAM
low complexity region	313	330	N/A	INTRINSIC
low complexity region	337	356	N/A	INTRINSIC
Pfam:E1-E2_ATPase	373	488	1.4e-13	PFAM
Pfam:Hydrolase	493	832	8.1e-16	PFAM
Pfam:HAD	496	829	6.3e-21	PFAM
Pfam:Cation_ATPase	542	638	4.4e-17	PFAM
Pfam:Hydrolase_3	791	865	8.3e-7	PFAM
transmembrane domain	878	900	N/A	INTRINSIC
Pfam:Cation_ATPase_C	902	1084	2.5e-47	PFAM
low complexity region	1102	1115	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1126	1178	2.4e-30	PFAM
low complexity region	1196	1211	N/A	INTRINSIC
low complexity region	1220	1234	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101045
AA Change: L925P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000098606
Gene: ENSMUSG00000030302
AA Change: L925P

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	156	444	1.7e-56	PFAM
Pfam:Hydrolase	448	787	3.9e-25	PFAM
Pfam:HAD	451	784	2.4e-16	PFAM
Pfam:Hydrolase_like2	497	593	9.4e-17	PFAM
Pfam:Hydrolase_3	745	820	1.7e-6	PFAM
transmembrane domain	833	855	N/A	INTRINSIC
Pfam:Cation_ATPase_C	857	1039	7.3e-46	PFAM
low complexity region	1057	1070	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1081	1144	1.4e-31	PFAM
low complexity region	1151	1166	N/A	INTRINSIC
low complexity region	1175	1189	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000152831
AA Change: L925P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000138165
Gene: ENSMUSG00000030302
AA Change: L925P

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	156	444	6.1e-57	PFAM
Pfam:Hydrolase	448	787	1.4e-25	PFAM
Pfam:HAD	451	784	7.7e-17	PFAM
Pfam:Hydrolase_like2	497	593	4.4e-17	PFAM
Pfam:Hydrolase_3	745	820	4.2e-7	PFAM
transmembrane domain	833	855	N/A	INTRINSIC
Pfam:Cation_ATPase_C	857	1039	2.7e-46	PFAM
low complexity region	1057	1070	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1081	1149	1.3e-19	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000205052
AA Change: L921P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000145174
Gene: ENSMUSG00000030302
AA Change: L921P

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	155	310	1.9e-28	PFAM
Pfam:E1-E2_ATPase	328	443	1.1e-13	PFAM
Pfam:HAD	451	780	2.7e-19	PFAM
Pfam:Cation_ATPase	497	593	5.8e-17	PFAM
Pfam:Hydrolase	576	783	2e-8	PFAM
Pfam:Hydrolase_3	711	816	2.3e-7	PFAM
transmembrane domain	829	851	N/A	INTRINSIC
Pfam:Cation_ATPase_C	853	1035	2.5e-47	PFAM
low complexity region	1053	1066	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1077	1129	2.6e-30	PFAM
low complexity region	1147	1162	N/A	INTRINSIC
low complexity region	1171	1185	N/A	INTRINSIC

Meta Mutation Damage Score

0.9745

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

98% (93/95)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutants exhibit slower growth, balance problems, and deafness, associated with cerebellar abnormalities, an absence of otoconia, and abnormalities of the organ of Corti. Heterozygotes exhibit appreciable age-dependent hearing loss. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930449I24Rik	T	A	5: 146,441,752 (GRCm39)	S300T	possibly damaging	Het
A2ml1	A	C	6: 128,535,400 (GRCm39)	V770G	probably damaging	Het
Abca6	A	G	11: 110,110,475 (GRCm39)	I558T	probably benign	Het
Abhd17c	C	A	7: 83,759,870 (GRCm39)	G295W	probably damaging	Het
Actn3	G	A	19: 4,921,703 (GRCm39)	A159V	probably damaging	Het
Adgrv1	A	T	13: 81,705,199 (GRCm39)	F1537Y	probably damaging	Het
Agbl1	G	T	7: 76,069,628 (GRCm39)	V373F	possibly damaging	Het
Aldh1a1	A	T	19: 20,620,279 (GRCm39)	E485D	probably benign	Het
Amdhd2	A	G	17: 24,377,282 (GRCm39)		probably null	Het
Armc3	A	G	2: 19,206,622 (GRCm39)	D15G	probably damaging	Het
Btbd16	A	G	7: 130,386,038 (GRCm39)	N88S	probably benign	Het
Capn10	T	A	1: 92,865,756 (GRCm39)	C77*	probably null	Het
Caskin1	T	C	17: 24,715,970 (GRCm39)		probably null	Het
Catsper4	T	C	4: 133,941,117 (GRCm39)	D254G	probably benign	Het
Catsperg1	T	C	7: 28,884,465 (GRCm39)	D958G	probably damaging	Het
Ccar2	T	G	14: 70,377,100 (GRCm39)	K787Q	probably benign	Het
Ccdc191	C	T	16: 43,728,998 (GRCm39)	T244I	probably benign	Het
Cd33	A	T	7: 43,179,699 (GRCm39)	L243Q	possibly damaging	Het
Cdc37	T	C	9: 21,061,143 (GRCm39)	Y4C	probably damaging	Het
Cenpe	T	G	3: 134,945,541 (GRCm39)	N1018K	probably benign	Het
Crocc	G	A	4: 140,744,407 (GRCm39)	R1830C	probably damaging	Het
Csmd1	T	C	8: 15,967,392 (GRCm39)	D3157G	probably damaging	Het
Dhx57	C	T	17: 80,580,477 (GRCm39)	V492M	probably damaging	Het
Dnah2	A	T	11: 69,349,011 (GRCm39)	I2486N	probably benign	Het
Dnhd1	C	T	7: 105,342,928 (GRCm39)	T1424I	possibly damaging	Het
Dsc3	C	T	18: 20,101,411 (GRCm39)	A661T	probably benign	Het
F930015N05Rik	A	G	11: 64,326,229 (GRCm39)		probably benign	Het
Fbxo34	C	A	14: 47,767,563 (GRCm39)	R308S	probably damaging	Het
Gm10608	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	9: 118,989,784 (GRCm39)		probably benign	Het
Gm11595	A	T	11: 99,663,327 (GRCm39)	C118S	unknown	Het
Gm9742	A	T	13: 8,085,011 (GRCm39)		noncoding transcript	Het
Gmeb2	G	A	2: 180,900,842 (GRCm39)	A185V	probably benign	Het
Gpr15	A	G	16: 58,538,618 (GRCm39)	V157A	possibly damaging	Het
Gpr3	C	T	4: 132,937,932 (GRCm39)	A247T	probably damaging	Het
Grin2b	A	C	6: 135,755,698 (GRCm39)	S539A	probably benign	Het
Hmbs	T	C	9: 44,252,004 (GRCm39)	T92A	probably benign	Het
Inpp4a	A	G	1: 37,406,000 (GRCm39)	M173V	probably benign	Het
Irx4	T	A	13: 73,413,595 (GRCm39)	S22T	probably benign	Het
Jph3	C	T	8: 122,511,881 (GRCm39)	A623V	probably benign	Het
Kif1b	G	A	4: 149,272,097 (GRCm39)	S1568L	possibly damaging	Het
Ksr1	G	A	11: 78,924,139 (GRCm39)	S361L	probably damaging	Het
Lyst	T	G	13: 13,809,847 (GRCm39)	Y506D	probably damaging	Het
Mctp1	A	G	13: 76,972,941 (GRCm39)	D648G	probably damaging	Het
Megf8	T	C	7: 25,064,007 (GRCm39)	S2788P	possibly damaging	Het
Mfsd2b	T	A	12: 4,917,659 (GRCm39)	Y129F	probably benign	Het
Mindy4	T	C	6: 55,195,250 (GRCm39)	S155P	probably benign	Het
Mospd4	A	G	18: 46,598,731 (GRCm39)		noncoding transcript	Het
Myo18b	A	T	5: 112,978,944 (GRCm39)	L1223Q	probably damaging	Het
Nckipsd	A	G	9: 108,688,932 (GRCm39)	T156A	probably benign	Het
Ndst1	G	A	18: 60,824,280 (GRCm39)	T799I	probably benign	Het
Npffr2	A	T	5: 89,715,924 (GRCm39)	I84F	probably damaging	Het
Nphp3	G	A	9: 103,885,442 (GRCm39)	V167M	probably damaging	Het
Nup107	C	A	10: 117,610,380 (GRCm39)	R354L	possibly damaging	Het
Olfml2a	T	C	2: 38,831,699 (GRCm39)	C93R	probably damaging	Het
Or12e10	A	G	2: 87,640,685 (GRCm39)	I174V	probably benign	Het
Or1e25	C	G	11: 73,493,631 (GRCm39)	S75C	possibly damaging	Het
Or5l14	A	T	2: 87,793,176 (GRCm39)	V20D	probably benign	Het
Pappa	T	A	4: 65,215,494 (GRCm39)	L1134M	probably damaging	Het
Plscr4	T	G	9: 92,370,683 (GRCm39)	F217V	possibly damaging	Het
Pnpla8	T	A	12: 44,354,840 (GRCm39)	Y667N	probably benign	Het
Polg	A	G	7: 79,114,676 (GRCm39)	L95P	probably damaging	Het
Psg20	T	G	7: 18,416,643 (GRCm39)	I158L	probably damaging	Het
Pwwp2a	A	G	11: 43,596,145 (GRCm39)	S437G	probably benign	Het
Rdx	A	G	9: 51,981,032 (GRCm39)	M305V	possibly damaging	Het
Rinl	A	G	7: 28,496,168 (GRCm39)	E383G	probably damaging	Het
Ror1	A	G	4: 100,299,290 (GRCm39)	M888V	probably benign	Het
Rps12	A	T	10: 23,662,776 (GRCm39)	I22K	possibly damaging	Het
Rtca	C	A	3: 116,291,323 (GRCm39)	R219L	possibly damaging	Het
Ryr2	G	A	13: 11,727,081 (GRCm39)	P2427S	probably damaging	Het
Slc10a6	A	G	5: 103,756,922 (GRCm39)	Y281H	probably benign	Het
Slc39a11	A	G	11: 113,260,629 (GRCm39)	S176P	probably benign	Het
Slfn10-ps	A	G	11: 82,921,168 (GRCm39)		noncoding transcript	Het
Spef2	T	C	15: 9,729,747 (GRCm39)	T124A	possibly damaging	Het
Sult2a4	G	T	7: 13,649,185 (GRCm39)	P207Q	probably damaging	Het
Tas1r3	G	A	4: 155,944,927 (GRCm39)	R765C	probably damaging	Het
Tcstv1a	T	C	13: 120,355,282 (GRCm39)	T117A	probably damaging	Het
Tha1	A	G	11: 117,760,600 (GRCm39)	V208A	probably damaging	Het
Tmbim4	T	A	10: 120,060,658 (GRCm39)	I215N	probably damaging	Het
Tmem202	T	A	9: 59,427,483 (GRCm39)	I122F	probably benign	Het
Tomm70a	T	C	16: 56,942,234 (GRCm39)	S4P	unknown	Het
Tpcn2	A	G	7: 144,827,712 (GRCm39)		probably benign	Het
Trim36	A	T	18: 46,345,404 (GRCm39)	F10I	probably benign	Het
Usp30	A	G	5: 114,249,224 (GRCm39)	E176G	probably damaging	Het
Usp8	T	G	2: 126,579,495 (GRCm39)		probably null	Het
Vmn1r32	T	A	6: 66,530,533 (GRCm39)	Y81F	probably benign	Het
Vps36	G	T	8: 22,708,305 (GRCm39)		probably null	Het
Wdr87-ps	T	C	7: 29,236,565 (GRCm39)		noncoding transcript	Het
Wnt3	A	G	11: 103,703,474 (GRCm39)	H319R	possibly damaging	Het
Zfp319	A	T	8: 96,050,391 (GRCm39)		probably benign	Het
Zfp408	T	C	2: 91,475,519 (GRCm39)	E545G	probably damaging	Het
Zfp799	C	T	17: 33,038,472 (GRCm39)	R598Q	possibly damaging	Het
Zfp831	T	C	2: 174,489,917 (GRCm39)	V1228A	probably benign	Het
Zfp938	T	C	10: 82,061,876 (GRCm39)	D248G	probably benign	Het

Other mutations in Atp2b2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00802:Atp2b2	APN	6	113,782,476 (GRCm39)	missense	possibly damaging	0.69
IGL01140:Atp2b2	APN	6	113,766,932 (GRCm39)	missense	possibly damaging	0.94
IGL02065:Atp2b2	APN	6	113,790,828 (GRCm39)	missense	probably damaging	1.00
IGL02267:Atp2b2	APN	6	113,770,691 (GRCm39)	missense	probably damaging	1.00
IGL02383:Atp2b2	APN	6	113,790,903 (GRCm39)	missense	probably damaging	0.99
IGL02498:Atp2b2	APN	6	113,770,815 (GRCm39)	missense	probably damaging	0.99
IGL02631:Atp2b2	APN	6	113,725,506 (GRCm39)	missense	probably damaging	1.00
IGL03028:Atp2b2	APN	6	113,736,103 (GRCm39)	missense	probably damaging	0.99
IGL03221:Atp2b2	APN	6	113,737,820 (GRCm39)	splice site	probably benign
IGL03290:Atp2b2	APN	6	113,770,715 (GRCm39)	missense	probably damaging	1.00
johan	UTSW	6	113,750,349 (GRCm39)	missense	probably damaging	1.00
lohan	UTSW	6	113,737,611 (GRCm39)	missense	probably damaging	1.00
IGL02799:Atp2b2	UTSW	6	113,739,813 (GRCm39)	nonsense	probably null
R0116:Atp2b2	UTSW	6	113,770,656 (GRCm39)	missense	probably damaging	1.00
R0131:Atp2b2	UTSW	6	113,770,743 (GRCm39)	missense	probably damaging	1.00
R0131:Atp2b2	UTSW	6	113,770,743 (GRCm39)	missense	probably damaging	1.00
R0132:Atp2b2	UTSW	6	113,770,743 (GRCm39)	missense	probably damaging	1.00
R0195:Atp2b2	UTSW	6	113,770,835 (GRCm39)	missense	probably benign	0.07
R0421:Atp2b2	UTSW	6	113,790,849 (GRCm39)	missense	probably damaging	1.00
R0791:Atp2b2	UTSW	6	113,750,349 (GRCm39)	missense	probably damaging	1.00
R0792:Atp2b2	UTSW	6	113,750,349 (GRCm39)	missense	probably damaging	1.00
R1033:Atp2b2	UTSW	6	113,770,849 (GRCm39)	splice site	probably null
R1248:Atp2b2	UTSW	6	113,794,153 (GRCm39)	missense	probably damaging	1.00
R1524:Atp2b2	UTSW	6	113,751,162 (GRCm39)	splice site	probably benign
R1809:Atp2b2	UTSW	6	113,780,704 (GRCm39)	intron	probably benign
R1829:Atp2b2	UTSW	6	113,750,329 (GRCm39)	missense	probably damaging	1.00
R1854:Atp2b2	UTSW	6	113,819,244 (GRCm39)	missense	probably damaging	1.00
R2138:Atp2b2	UTSW	6	113,773,268 (GRCm39)	missense	probably benign	0.21
R2351:Atp2b2	UTSW	6	113,766,718 (GRCm39)	missense	possibly damaging	0.91
R3923:Atp2b2	UTSW	6	113,774,069 (GRCm39)	critical splice donor site	probably null
R3951:Atp2b2	UTSW	6	113,737,792 (GRCm39)	missense	possibly damaging	0.51
R4178:Atp2b2	UTSW	6	113,770,679 (GRCm39)	missense	probably damaging	1.00
R4353:Atp2b2	UTSW	6	113,742,745 (GRCm39)	missense	probably benign	0.01
R4578:Atp2b2	UTSW	6	113,737,672 (GRCm39)	missense	probably damaging	1.00
R4797:Atp2b2	UTSW	6	113,766,847 (GRCm39)	missense	possibly damaging	0.92
R4884:Atp2b2	UTSW	6	113,819,147 (GRCm39)	missense	possibly damaging	0.65
R4976:Atp2b2	UTSW	6	113,736,122 (GRCm39)	missense	probably damaging	1.00
R5273:Atp2b2	UTSW	6	113,736,193 (GRCm39)	missense	probably damaging	1.00
R5350:Atp2b2	UTSW	6	113,736,199 (GRCm39)	missense	probably damaging	0.99
R5414:Atp2b2	UTSW	6	113,819,102 (GRCm39)	missense	probably damaging	1.00
R5560:Atp2b2	UTSW	6	113,751,319 (GRCm39)	missense	possibly damaging	0.90
R5589:Atp2b2	UTSW	6	113,751,400 (GRCm39)	missense	possibly damaging	0.94
R5790:Atp2b2	UTSW	6	113,736,270 (GRCm39)	missense	probably damaging	0.97
R6001:Atp2b2	UTSW	6	113,770,728 (GRCm39)	missense	probably damaging	1.00
R6127:Atp2b2	UTSW	6	113,790,838 (GRCm39)	missense	probably damaging	1.00
R6331:Atp2b2	UTSW	6	113,774,092 (GRCm39)	missense	probably benign	0.01
R6925:Atp2b2	UTSW	6	113,737,681 (GRCm39)	missense	probably damaging	1.00
R7231:Atp2b2	UTSW	6	113,742,693 (GRCm39)	missense	possibly damaging	0.89
R8219:Atp2b2	UTSW	6	113,770,811 (GRCm39)	missense	probably damaging	1.00
R8233:Atp2b2	UTSW	6	113,742,680 (GRCm39)	critical splice donor site	probably null
R8286:Atp2b2	UTSW	6	113,819,275 (GRCm39)	missense	possibly damaging	0.64
R8369:Atp2b2	UTSW	6	113,790,747 (GRCm39)	critical splice donor site	probably null
R8444:Atp2b2	UTSW	6	113,770,772 (GRCm39)	missense	probably benign	0.18
R8942:Atp2b2	UTSW	6	113,790,991 (GRCm39)	missense	probably benign	0.00
R8953:Atp2b2	UTSW	6	113,737,630 (GRCm39)	missense	possibly damaging	0.82
R8977:Atp2b2	UTSW	6	113,750,325 (GRCm39)	missense	probably damaging	1.00
R9051:Atp2b2	UTSW	6	113,740,566 (GRCm39)	missense	probably damaging	1.00
R9399:Atp2b2	UTSW	6	113,780,713 (GRCm39)	missense	probably benign
R9648:Atp2b2	UTSW	6	113,780,707 (GRCm39)	critical splice donor site	probably null
X0020:Atp2b2	UTSW	6	113,782,461 (GRCm39)	missense	probably damaging	1.00
X0020:Atp2b2	UTSW	6	113,782,460 (GRCm39)	missense	probably damaging	1.00
Z1088:Atp2b2	UTSW	6	113,819,267 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACACTACTGCTGGCTTTGAGG -3'
(R):5'- AGATGCTCTGGGTGAACCTC -3'

Sequencing Primer
(F):5'- ACAGGGCATGACAACTTCTG -3'
(R):5'- GTGAACCTCATCATGGACACGTTTG -3'

Posted On

2014-09-17