|Institutional Source||Beutler Lab|
|Gene Name||galanin receptor 2|
|Is this an essential gene?||Probably non essential (E-score: 0.105)|
|Stock #||R2059 (G1)|
|Chromosomal Location||116280939-116283938 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 116282939 bp|
|Amino Acid Change||Serine to Proline at position 132 (S132P)|
|Ref Sequence||ENSEMBL: ENSMUSP00000054062 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000055872]|
|Predicted Effect||probably damaging
AA Change: S132P
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: S132P
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.224|
|Coding Region Coverage||
|Validation Efficiency||99% (99/100)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene display a reduction in exploratory activity. There is also a modest shift in the distribution of different lymphocyte cell types. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Galr2||
(F):5'- CCTCAGGCATTAGAGATCTGTC -3'
(R):5'- AAAGTGCAGAGGTCCATGGC -3'
(F):5'- TCCCTCGGTTGTCACAAGAG -3'
(R):5'- AGAGGTCCATGGCGCGAC -3'