Incidental Mutation 'R2103:Olfr459'
ID230676
Institutional Source Beutler Lab
Gene Symbol Olfr459
Ensembl Gene ENSMUSG00000045479
Gene Nameolfactory receptor 459
SynonymsGA_x6K02T2P3E9-5780974-5781915, MOR120-1
MMRRC Submission 040107-MU
Accession Numbers
Is this an essential gene? Possibly non essential (E-score: 0.483) question?
Stock #R2103 (G1)
Quality Score225
Status Not validated
Chromosome6
Chromosomal Location41768770-41775177 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 41772005 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Threonine at position 98 (I98T)
Ref Sequence ENSEMBL: ENSMUSP00000149928 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000050412] [ENSMUST00000214752] [ENSMUST00000214976]
Predicted Effect probably benign
Transcript: ENSMUST00000050412
AA Change: I98T

PolyPhen 2 Score 0.360 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000061622
Gene: ENSMUSG00000045479
AA Change: I98T

DomainStartEndE-ValueType
Pfam:7tm_4 30 307 7.9e-46 PFAM
Pfam:7TM_GPCR_Srsx 34 304 1.7e-6 PFAM
Pfam:7tm_1 40 290 6.2e-24 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000203430
AA Change: I98T
SMART Domains Protein: ENSMUSP00000145036
Gene: ENSMUSG00000045479
AA Change: I98T

DomainStartEndE-ValueType
Pfam:7tm_4 30 307 7.9e-46 PFAM
Pfam:7TM_GPCR_Srsx 34 304 1.7e-6 PFAM
Pfam:7tm_1 40 290 6.2e-24 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000214752
AA Change: I98T

PolyPhen 2 Score 0.360 (Sensitivity: 0.90; Specificity: 0.89)
Predicted Effect probably benign
Transcript: ENSMUST00000214976
AA Change: I98T

PolyPhen 2 Score 0.360 (Sensitivity: 0.90; Specificity: 0.89)
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.2%
  • 20x: 94.9%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 88 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aadac C A 3: 60,039,814 P311Q probably damaging Het
Acoxl T A 2: 127,972,606 M314K probably damaging Het
Agmat A T 4: 141,755,903 D216V probably damaging Het
Aida A T 1: 183,313,692 E107D probably benign Het
Ano5 A G 7: 51,537,813 K50R possibly damaging Het
Anxa2 A T 9: 69,483,816 D95V probably damaging Het
Aspm G A 1: 139,491,665 V3023M probably damaging Het
Atg16l2 A G 7: 101,290,361 probably null Het
B3galt4 G A 17: 33,950,839 R142C probably damaging Het
B3galt5 A T 16: 96,316,025 K286M probably damaging Het
Best3 A C 10: 117,002,594 I186L probably benign Het
Blm G T 7: 80,505,949 probably null Het
Cat A T 2: 103,463,315 D389E probably damaging Het
Cluh C A 11: 74,659,529 C222* probably null Het
Cntnap2 G A 6: 47,298,588 E1325K probably damaging Het
Col14a1 A T 15: 55,449,940 D1320V unknown Het
Col4a3bp T A 13: 96,634,886 N550K probably damaging Het
Cpne7 A G 8: 123,127,437 K288E possibly damaging Het
Cyp26b1 A G 6: 84,575,050 S369P possibly damaging Het
Cyp2j9 T A 4: 96,571,964 K434M probably damaging Het
Dpyd G C 3: 119,064,952 S605T probably benign Het
Dst A G 1: 34,190,258 T1986A probably benign Het
Ebf2 T A 14: 67,387,942 V233D probably damaging Het
Ecm2 A G 13: 49,530,256 D570G probably benign Het
Efhc1 T A 1: 20,989,560 C611* probably null Het
Epop T C 11: 97,628,654 T210A probably benign Het
Fdxacb1 A T 9: 50,771,646 N101I probably benign Het
Fezf1 A G 6: 23,247,332 F248S possibly damaging Het
Galnt16 A G 12: 80,583,656 D262G probably damaging Het
Gm9507 T A 10: 77,811,666 probably benign Het
Grin2b A T 6: 135,780,140 I441N probably benign Het
H2-Eb2 A G 17: 34,334,304 I155V probably benign Het
Hectd4 C A 5: 121,355,629 D3811E probably benign Het
Herc4 T C 10: 63,246,110 S71P probably benign Het
Hhipl1 A G 12: 108,327,718 T628A probably benign Het
Hoga1 A C 19: 42,060,020 probably null Het
Igf2bp1 A G 11: 95,975,296 V122A probably damaging Het
Il10ra C A 9: 45,255,811 A481S probably benign Het
Klk1b26 A T 7: 44,016,900 T256S probably damaging Het
Kndc1 C T 7: 139,921,234 T813I probably benign Het
Limch1 A G 5: 66,998,729 K394R probably benign Het
Lrrc37a T C 11: 103,500,261 E1446G probably benign Het
Lrrc47 C T 4: 154,015,893 R287W probably damaging Het
Mdn1 T A 4: 32,738,712 L3555Q possibly damaging Het
Mei1 A G 15: 82,103,204 H399R possibly damaging Het
Mei1 G T 15: 82,107,036 V472F probably damaging Het
Mrps34 T A 17: 24,895,490 probably null Het
Myom3 A G 4: 135,776,412 T391A probably benign Het
Nfib G A 4: 82,330,408 T314I possibly damaging Het
Olfr1451 T C 19: 12,999,502 V172A possibly damaging Het
Olfr498 A G 7: 108,465,603 N93S probably benign Het
Olfr774 T C 10: 129,238,499 S117P probably damaging Het
Pdia3 G C 2: 121,433,993 G346A probably damaging Het
Plce1 T C 19: 38,777,924 F2117S probably damaging Het
Plec A G 15: 76,173,543 F4055L probably damaging Het
Ppip5k1 A T 2: 121,321,653 probably null Het
Psma6 T C 12: 55,408,057 I57T probably benign Het
Psme2 A T 14: 55,590,840 probably null Het
Reln A T 5: 21,969,360 D1948E possibly damaging Het
Rsf1 GGCG GGCGACGGCAGCG 7: 97,579,906 probably benign Het
Sbno1 G T 5: 124,393,937 S727R probably damaging Het
Serpina3m C A 12: 104,389,699 Y208* probably null Het
Serpind1 T C 16: 17,342,944 V446A probably benign Het
Shc3 T A 13: 51,442,836 M384L probably benign Het
Slc38a11 A G 2: 65,330,339 F304L probably benign Het
Slc4a5 A C 6: 83,224,681 D4A probably benign Het
Slc4a5 G A 6: 83,297,378 A1076T probably benign Het
Slpi C T 2: 164,355,543 C28Y probably damaging Het
Sptan1 T C 2: 30,030,471 S2320P probably damaging Het
Stim2 A G 5: 54,105,249 T278A possibly damaging Het
Sympk T A 7: 19,054,116 S1186T probably benign Het
Tbrg1 T C 9: 37,649,419 D387G probably benign Het
Tns2 A T 15: 102,112,665 probably null Het
Tnxb A G 17: 34,682,251 Y1013C probably damaging Het
Tpsg1 T C 17: 25,373,293 S41P possibly damaging Het
Trim36 T C 18: 46,196,082 N85S probably benign Het
Trpm6 A T 19: 18,796,284 H380L probably benign Het
Tssk4 A G 14: 55,651,540 I174M probably damaging Het
Ttn C T 2: 76,946,391 probably null Het
Vmn2r114 ATTT ATT 17: 23,290,932 probably null Het
Vmn2r4 T A 3: 64,415,283 N5I possibly damaging Het
Vps11 G A 9: 44,359,227 H183Y probably damaging Het
Vsig10l A G 7: 43,467,468 T476A possibly damaging Het
Vwa8 C T 14: 78,908,230 R116C probably damaging Het
Vwf G A 6: 125,646,330 V1797I probably benign Het
Wasl A T 6: 24,618,378 S447T unknown Het
Whamm C T 7: 81,591,771 R277* probably null Het
Zfp874a T A 13: 67,442,504 I354F probably benign Het
Other mutations in Olfr459
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00896:Olfr459 APN 6 41772113 missense probably damaging 1.00
IGL01460:Olfr459 APN 6 41772282 missense probably benign 0.12
IGL02465:Olfr459 APN 6 41771556 missense probably damaging 1.00
R0352:Olfr459 UTSW 6 41772124 missense probably damaging 0.99
R0443:Olfr459 UTSW 6 41771895 missense possibly damaging 0.75
R0480:Olfr459 UTSW 6 41772264 missense probably benign 0.01
R1491:Olfr459 UTSW 6 41771522 missense possibly damaging 0.95
R1495:Olfr459 UTSW 6 41771903 missense probably damaging 1.00
R3688:Olfr459 UTSW 6 41772226 nonsense probably null
R3910:Olfr459 UTSW 6 41772149 missense probably benign 0.00
R4709:Olfr459 UTSW 6 41771508 missense probably benign 0.06
R4710:Olfr459 UTSW 6 41771508 missense probably benign 0.06
R4960:Olfr459 UTSW 6 41772069 missense probably damaging 0.97
R5162:Olfr459 UTSW 6 41771772 missense possibly damaging 0.80
R5236:Olfr459 UTSW 6 41772111 missense probably benign 0.00
R5441:Olfr459 UTSW 6 41771848 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- ACTGGAATGTGGCATAGACTG -3'
(R):5'- TTCTATTCAGTGACACTGCTGGG -3'

Sequencing Primer
(F):5'- GGCATAGACTGGCCAGATTTC -3'
(R):5'- CTGCTGGGAAACATGGTCATCATC -3'
Posted On2014-09-18