Mutagenetix > Incidental Mutation

Incidental Mutation 'R2096:Or5g25'

232084

Institutional Source

Beutler Lab

Gene Symbol

Or5g25

Ensembl Gene

ENSMUSG00000075214

Gene Name

olfactory receptor family 5 subfamily G member 25

Synonyms

MOR175-2, Olfr1002, GA_x6K02T2Q125-47127746-47126790

MMRRC Submission

040100-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R2096 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85477707-85478663 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85478434 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 77 (I77T)

Ref Sequence

ENSEMBL: ENSMUSP00000150405 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099920] [ENSMUST00000215548]

AlphaFold

Q8VFK2

Predicted Effect

probably benign
Transcript: ENSMUST00000099920
AA Change: I77T

PolyPhen 2 Score 0.196 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000097504
Gene: ENSMUSG00000075214
AA Change: I77T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.7e-50	PFAM
Pfam:7tm_1	41	290	2.5e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215548
AA Change: I77T

PolyPhen 2 Score 0.196 (Sensitivity: 0.92; Specificity: 0.87)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb10	A	G	8: 124,709,195 (GRCm39)	S120P	probably benign	Het
Actr3b	T	A	5: 26,036,743 (GRCm39)	Y185*	probably null	Het
Atg101	T	A	15: 101,188,382 (GRCm39)	H162Q	possibly damaging	Het
Btf3	T	A	13: 98,449,659 (GRCm39)	Q109L	possibly damaging	Het
Camk2d	G	A	3: 126,574,091 (GRCm39)	G166D	probably damaging	Het
Capn7	T	G	14: 31,071,844 (GRCm39)		probably null	Het
Cd209f	A	G	8: 4,155,537 (GRCm39)	V17A	probably benign	Het
Cenpf	A	G	1: 189,385,656 (GRCm39)	V2208A	possibly damaging	Het
Cnga1	C	T	5: 72,776,404 (GRCm39)	V20I	possibly damaging	Het
Comp	T	C	8: 70,828,713 (GRCm39)	C182R	probably damaging	Het
Ctsl	A	G	13: 64,516,840 (GRCm39)		probably null	Het
Cyp3a44	G	T	5: 145,725,215 (GRCm39)	Q329K	probably damaging	Het
Dcpp1	A	T	17: 24,099,971 (GRCm39)		probably null	Het
Dhx38	A	G	8: 110,280,891 (GRCm39)	V831A	probably damaging	Het
Dkk2	T	C	3: 131,791,858 (GRCm39)	M22T	probably benign	Het
Dnah2	C	T	11: 69,346,742 (GRCm39)	E2615K	probably damaging	Het
Dpy19l3	A	G	7: 35,426,713 (GRCm39)		probably null	Het
Gm12888	T	G	4: 121,176,655 (GRCm39)	I49L	probably benign	Het
Insyn2b	C	A	11: 34,352,936 (GRCm39)	S326Y	probably benign	Het
Kif15	T	A	9: 122,815,252 (GRCm39)	M194K	probably damaging	Het
Mael	G	T	1: 166,053,244 (GRCm39)	Q254K	probably benign	Het
Nek9	T	C	12: 85,361,322 (GRCm39)	D461G	probably benign	Het
Nlrp12	T	C	7: 3,281,825 (GRCm39)	N819S	probably benign	Het
Ntng1	T	A	3: 109,739,871 (GRCm39)	N390Y	probably damaging	Het
P2ry14	C	T	3: 59,022,738 (GRCm39)	V250I	probably damaging	Het
Pcnx2	A	T	8: 126,485,987 (GRCm39)	D1870E	probably benign	Het
Phf2	T	A	13: 48,985,589 (GRCm39)	K64*	probably null	Het
Phip	T	C	9: 82,797,392 (GRCm39)	H537R	possibly damaging	Het
Ppa2	A	G	3: 133,032,445 (GRCm39)	T97A	probably damaging	Het
Psd	G	A	19: 46,313,088 (GRCm39)		probably null	Het
Psg23	T	A	7: 18,348,668 (GRCm39)	E46D	probably damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Rab11b	A	G	17: 33,967,976 (GRCm39)	Y74H	probably damaging	Het
Rfc3	A	T	5: 151,568,383 (GRCm39)	S194R	probably benign	Het
Sema4d	A	T	13: 51,864,037 (GRCm39)	I414N	probably damaging	Het
Serpinb1a	A	T	13: 33,031,437 (GRCm39)	D120E	probably damaging	Het
Snai2	T	A	16: 14,524,861 (GRCm39)	H122Q	possibly damaging	Het
Spats2l	T	C	1: 57,985,458 (GRCm39)	S504P	probably benign	Het
Sprr2j-ps	T	C	3: 92,326,412 (GRCm39)	S96P	unknown	Het
Sspo	G	T	6: 48,438,608 (GRCm39)	V1559L	probably benign	Het
Tas2r118	T	G	6: 23,969,912 (GRCm39)	I50L	possibly damaging	Het
Tdrd3	C	T	14: 87,743,788 (GRCm39)	Q573*	probably null	Het
Thoc2l	T	C	5: 104,667,835 (GRCm39)	S786P	possibly damaging	Het
Tlnrd1	G	A	7: 83,532,062 (GRCm39)	T123I	probably benign	Het
Tmem214	T	A	5: 31,033,714 (GRCm39)	L555H	probably damaging	Het
Tmprss12	T	A	15: 100,183,117 (GRCm39)	M153K	probably benign	Het
Tmprss9	G	A	10: 80,725,268 (GRCm39)	G410S	probably damaging	Het
Trpm6	T	A	19: 18,803,116 (GRCm39)	M853K	probably damaging	Het
Vmn1r35	A	G	6: 66,655,929 (GRCm39)	I247T	possibly damaging	Het
Zcchc7	C	A	4: 44,931,059 (GRCm39)	P404T	probably damaging	Het
Zzef1	T	C	11: 72,763,465 (GRCm39)	F1371L	probably benign	Het

Other mutations in Or5g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02591:Or5g25	APN	2	85,478,487 (GRCm39)	missense	probably damaging	0.98
IGL02873:Or5g25	APN	2	85,478,096 (GRCm39)	missense	possibly damaging	0.50
PIT4362001:Or5g25	UTSW	2	85,478,068 (GRCm39)	missense	probably damaging	1.00
R1241:Or5g25	UTSW	2	85,477,904 (GRCm39)	missense	probably damaging	1.00
R1261:Or5g25	UTSW	2	85,478,143 (GRCm39)	missense	probably damaging	1.00
R1666:Or5g25	UTSW	2	85,478,157 (GRCm39)	nonsense	probably null
R1902:Or5g25	UTSW	2	85,478,201 (GRCm39)	missense	possibly damaging	0.81
R1965:Or5g25	UTSW	2	85,478,090 (GRCm39)	missense	possibly damaging	0.94
R4239:Or5g25	UTSW	2	85,478,647 (GRCm39)	missense	probably damaging	0.98
R4730:Or5g25	UTSW	2	85,478,336 (GRCm39)	missense	probably benign	0.39
R4948:Or5g25	UTSW	2	85,477,916 (GRCm39)	missense	probably benign	0.30
R5627:Or5g25	UTSW	2	85,477,991 (GRCm39)	missense	probably damaging	1.00
R5844:Or5g25	UTSW	2	85,478,239 (GRCm39)	missense	probably benign	0.36
R6809:Or5g25	UTSW	2	85,478,317 (GRCm39)	missense	probably damaging	1.00
R7399:Or5g25	UTSW	2	85,477,768 (GRCm39)	missense	possibly damaging	0.89
R7476:Or5g25	UTSW	2	85,478,512 (GRCm39)	missense	not run
R7805:Or5g25	UTSW	2	85,477,794 (GRCm39)	nonsense	probably null
R7960:Or5g25	UTSW	2	85,478,417 (GRCm39)	missense	possibly damaging	0.82
R8015:Or5g25	UTSW	2	85,478,136 (GRCm39)	missense	probably damaging	0.99
R8355:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8455:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8479:Or5g25	UTSW	2	85,478,447 (GRCm39)	missense	probably damaging	1.00
R8683:Or5g25	UTSW	2	85,478,410 (GRCm39)	missense	probably benign	0.35
R8699:Or5g25	UTSW	2	85,478,330 (GRCm39)	missense	possibly damaging	0.87
R8762:Or5g25	UTSW	2	85,478,034 (GRCm39)	missense	probably damaging	1.00
R8897:Or5g25	UTSW	2	85,478,187 (GRCm39)	missense	possibly damaging	0.92
R9280:Or5g25	UTSW	2	85,478,504 (GRCm39)	nonsense	probably null
R9674:Or5g25	UTSW	2	85,478,593 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- ACTCGCTGGGACATGATGAG -3'
(R):5'- TCAAACTGTTGTGACTGAGTTCTTC -3'

Sequencing Primer
(F):5'- CTCGCTGGGACATGATGAGAGTATAC -3'
(R):5'- ACTGAGTTCTTCTTCACTGGATTAAC -3'

Posted On

2014-09-18