Mutagenetix > Incidental Mutation

Incidental Mutation 'R2096:Vmn1r35'

232104

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r35

Ensembl Gene

ENSMUSG00000060699

Gene Name

vomeronasal 1 receptor 35

Synonyms

V1rc12

MMRRC Submission

040100-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R2096 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66655778-66656668 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 66655929 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 247 (I247T)

Ref Sequence

ENSEMBL: ENSMUSP00000154707 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071414] [ENSMUST00000227346] [ENSMUST00000227354] [ENSMUST00000227749] [ENSMUST00000227961]

AlphaFold

Q8R2E2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000071414
AA Change: I247T

PolyPhen 2 Score 0.943 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000071362
Gene: ENSMUSG00000060699
AA Change: I247T

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	1.2e-54	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000227346
AA Change: I80T

PolyPhen 2 Score 0.104 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000227354
AA Change: I247T

PolyPhen 2 Score 0.943 (Sensitivity: 0.80; Specificity: 0.95)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000227749
AA Change: I247T

PolyPhen 2 Score 0.943 (Sensitivity: 0.80; Specificity: 0.95)

Predicted Effect

probably benign
Transcript: ENSMUST00000227961
AA Change: I80T

PolyPhen 2 Score 0.104 (Sensitivity: 0.93; Specificity: 0.86)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb10	A	G	8: 124,709,195 (GRCm39)	S120P	probably benign	Het
Actr3b	T	A	5: 26,036,743 (GRCm39)	Y185*	probably null	Het
Atg101	T	A	15: 101,188,382 (GRCm39)	H162Q	possibly damaging	Het
Btf3	T	A	13: 98,449,659 (GRCm39)	Q109L	possibly damaging	Het
Camk2d	G	A	3: 126,574,091 (GRCm39)	G166D	probably damaging	Het
Capn7	T	G	14: 31,071,844 (GRCm39)		probably null	Het
Cd209f	A	G	8: 4,155,537 (GRCm39)	V17A	probably benign	Het
Cenpf	A	G	1: 189,385,656 (GRCm39)	V2208A	possibly damaging	Het
Cnga1	C	T	5: 72,776,404 (GRCm39)	V20I	possibly damaging	Het
Comp	T	C	8: 70,828,713 (GRCm39)	C182R	probably damaging	Het
Ctsl	A	G	13: 64,516,840 (GRCm39)		probably null	Het
Cyp3a44	G	T	5: 145,725,215 (GRCm39)	Q329K	probably damaging	Het
Dcpp1	A	T	17: 24,099,971 (GRCm39)		probably null	Het
Dhx38	A	G	8: 110,280,891 (GRCm39)	V831A	probably damaging	Het
Dkk2	T	C	3: 131,791,858 (GRCm39)	M22T	probably benign	Het
Dnah2	C	T	11: 69,346,742 (GRCm39)	E2615K	probably damaging	Het
Dpy19l3	A	G	7: 35,426,713 (GRCm39)		probably null	Het
Gm12888	T	G	4: 121,176,655 (GRCm39)	I49L	probably benign	Het
Insyn2b	C	A	11: 34,352,936 (GRCm39)	S326Y	probably benign	Het
Kif15	T	A	9: 122,815,252 (GRCm39)	M194K	probably damaging	Het
Mael	G	T	1: 166,053,244 (GRCm39)	Q254K	probably benign	Het
Nek9	T	C	12: 85,361,322 (GRCm39)	D461G	probably benign	Het
Nlrp12	T	C	7: 3,281,825 (GRCm39)	N819S	probably benign	Het
Ntng1	T	A	3: 109,739,871 (GRCm39)	N390Y	probably damaging	Het
Or5g25	A	G	2: 85,478,434 (GRCm39)	I77T	probably benign	Het
P2ry14	C	T	3: 59,022,738 (GRCm39)	V250I	probably damaging	Het
Pcnx2	A	T	8: 126,485,987 (GRCm39)	D1870E	probably benign	Het
Phf2	T	A	13: 48,985,589 (GRCm39)	K64*	probably null	Het
Phip	T	C	9: 82,797,392 (GRCm39)	H537R	possibly damaging	Het
Ppa2	A	G	3: 133,032,445 (GRCm39)	T97A	probably damaging	Het
Psd	G	A	19: 46,313,088 (GRCm39)		probably null	Het
Psg23	T	A	7: 18,348,668 (GRCm39)	E46D	probably damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Rab11b	A	G	17: 33,967,976 (GRCm39)	Y74H	probably damaging	Het
Rfc3	A	T	5: 151,568,383 (GRCm39)	S194R	probably benign	Het
Sema4d	A	T	13: 51,864,037 (GRCm39)	I414N	probably damaging	Het
Serpinb1a	A	T	13: 33,031,437 (GRCm39)	D120E	probably damaging	Het
Snai2	T	A	16: 14,524,861 (GRCm39)	H122Q	possibly damaging	Het
Spats2l	T	C	1: 57,985,458 (GRCm39)	S504P	probably benign	Het
Sprr2j-ps	T	C	3: 92,326,412 (GRCm39)	S96P	unknown	Het
Sspo	G	T	6: 48,438,608 (GRCm39)	V1559L	probably benign	Het
Tas2r118	T	G	6: 23,969,912 (GRCm39)	I50L	possibly damaging	Het
Tdrd3	C	T	14: 87,743,788 (GRCm39)	Q573*	probably null	Het
Thoc2l	T	C	5: 104,667,835 (GRCm39)	S786P	possibly damaging	Het
Tlnrd1	G	A	7: 83,532,062 (GRCm39)	T123I	probably benign	Het
Tmem214	T	A	5: 31,033,714 (GRCm39)	L555H	probably damaging	Het
Tmprss12	T	A	15: 100,183,117 (GRCm39)	M153K	probably benign	Het
Tmprss9	G	A	10: 80,725,268 (GRCm39)	G410S	probably damaging	Het
Trpm6	T	A	19: 18,803,116 (GRCm39)	M853K	probably damaging	Het
Zcchc7	C	A	4: 44,931,059 (GRCm39)	P404T	probably damaging	Het
Zzef1	T	C	11: 72,763,465 (GRCm39)	F1371L	probably benign	Het

Other mutations in Vmn1r35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00962:Vmn1r35	APN	6	66,656,361 (GRCm39)	missense	possibly damaging	0.90
IGL01417:Vmn1r35	APN	6	66,656,191 (GRCm39)	missense	probably benign	0.00
IGL01517:Vmn1r35	APN	6	66,656,434 (GRCm39)	missense	probably benign	0.01
IGL02142:Vmn1r35	APN	6	66,656,334 (GRCm39)	missense	probably damaging	1.00
IGL02178:Vmn1r35	APN	6	66,656,086 (GRCm39)	missense	probably damaging	1.00
IGL02383:Vmn1r35	APN	6	66,655,876 (GRCm39)	missense	probably damaging	1.00
IGL02383:Vmn1r35	APN	6	66,655,875 (GRCm39)	missense	probably damaging	1.00
IGL02493:Vmn1r35	APN	6	66,656,463 (GRCm39)	missense	possibly damaging	0.76
R0360:Vmn1r35	UTSW	6	66,655,827 (GRCm39)	missense	probably damaging	0.99
R0364:Vmn1r35	UTSW	6	66,655,827 (GRCm39)	missense	probably damaging	0.99
R0599:Vmn1r35	UTSW	6	66,656,497 (GRCm39)	missense	probably benign	0.06
R1447:Vmn1r35	UTSW	6	66,655,890 (GRCm39)	missense	probably benign	0.13
R1781:Vmn1r35	UTSW	6	66,656,550 (GRCm39)	missense	probably benign	0.24
R2937:Vmn1r35	UTSW	6	66,655,950 (GRCm39)	missense	possibly damaging	0.78
R2938:Vmn1r35	UTSW	6	66,655,950 (GRCm39)	missense	possibly damaging	0.78
R3937:Vmn1r35	UTSW	6	66,656,057 (GRCm39)	missense	probably damaging	1.00
R3938:Vmn1r35	UTSW	6	66,656,057 (GRCm39)	missense	probably damaging	1.00
R4386:Vmn1r35	UTSW	6	66,656,573 (GRCm39)	nonsense	probably null
R5329:Vmn1r35	UTSW	6	66,656,490 (GRCm39)	nonsense	probably null
R6638:Vmn1r35	UTSW	6	66,655,848 (GRCm39)	missense	possibly damaging	0.63
R7175:Vmn1r35	UTSW	6	66,655,906 (GRCm39)	missense	probably benign	0.06
R7448:Vmn1r35	UTSW	6	66,656,219 (GRCm39)	start gained	probably benign
R7825:Vmn1r35	UTSW	6	66,656,443 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn1r35	UTSW	6	66,655,933 (GRCm39)	missense	probably benign	0.10
R9415:Vmn1r35	UTSW	6	66,656,115 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- AATCTTGCTTTGCTGCTTGAAC -3'
(R):5'- TCATGCTGACTACGAGCACATAC -3'

Sequencing Primer
(F):5'- ATCCAGAGGATCATGTGAGCTCTTC -3'
(R):5'- CTGACTACGAGCACATACATGGTG -3'

Posted On

2014-09-18