Incidental Mutation 'R2158:Nid2'
ID 235009
Institutional Source Beutler Lab
Gene Symbol Nid2
Ensembl Gene ENSMUSG00000021806
Gene Name nidogen 2
Synonyms entactin 2, entactin-2
MMRRC Submission 040161-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.164) question?
Stock # R2158 (G1)
Quality Score 225
Status Validated
Chromosome 14
Chromosomal Location 19801333-19861855 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 19828111 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glycine to Serine at position 516 (G516S)
Ref Sequence ENSEMBL: ENSMUSP00000022340 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000022340]
AlphaFold O88322
Predicted Effect probably benign
Transcript: ENSMUST00000022340
AA Change: G516S

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000022340
Gene: ENSMUSG00000021806
AA Change: G516S

DomainStartEndE-ValueType
signal peptide 1 30 N/A INTRINSIC
Blast:NIDO 39 77 3e-11 BLAST
NIDO 108 276 1.12e-72 SMART
low complexity region 421 430 N/A INTRINSIC
low complexity region 452 470 N/A INTRINSIC
EGF 510 547 1.84e1 SMART
G2F 548 780 4.36e-143 SMART
EGF 785 823 2.52e-2 SMART
EGF_CA 824 866 1.45e-11 SMART
EGF 874 914 3.15e-3 SMART
EGF_CA 915 953 5.03e-11 SMART
TY 988 1037 8.27e-20 SMART
TY 1068 1116 1.19e-20 SMART
LY 1162 1204 1.15e-5 SMART
LY 1206 1248 8.82e-16 SMART
LY 1249 1293 1.51e-14 SMART
LY 1294 1336 3.56e-11 SMART
Predicted Effect unknown
Transcript: ENSMUST00000224263
AA Change: G250S
Predicted Effect noncoding transcript
Transcript: ENSMUST00000225791
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.6%
  • 20x: 96.0%
Validation Efficiency 100% (59/59)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the nidogen family of basement membrane proteins. This protein is a cell-adhesion protein that binds collagens I and IV and laminin and may be involved in maintaining the structure of the basement membrane.[provided by RefSeq, Jun 2010]
PHENOTYPE: Mice homozygous for a null alleleexhibit calcification of joint cartilage and osteoarthritis. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1810055G02Rik T C 19: 3,766,608 (GRCm39) V65A possibly damaging Het
4930402F06Rik A T 2: 35,275,680 (GRCm39) S38T possibly damaging Het
Adam4 A G 12: 81,468,537 (GRCm39) L28S probably damaging Het
Agmo T A 12: 37,407,709 (GRCm39) F198I probably damaging Het
Akap7 A G 10: 25,047,062 (GRCm39) V45A probably damaging Het
Amotl1 G T 9: 14,486,465 (GRCm39) N476K probably benign Het
Apcs A G 1: 172,722,100 (GRCm39) L82P probably damaging Het
Armc3 C A 2: 19,253,444 (GRCm39) P195Q probably damaging Het
Astn2 A T 4: 66,322,491 (GRCm39) L36Q unknown Het
Atad2 A T 15: 57,961,962 (GRCm39) S870T possibly damaging Het
Bmp10 A T 6: 87,411,062 (GRCm39) D285V probably benign Het
Caskin1 T C 17: 24,724,128 (GRCm39) V972A probably benign Het
Ccdc141 T A 2: 76,861,015 (GRCm39) N921Y probably damaging Het
Cntnap5b A T 1: 100,318,297 (GRCm39) D1019V probably damaging Het
Eml5 T C 12: 98,810,205 (GRCm39) probably benign Het
Evi5l T C 8: 4,243,195 (GRCm39) Y360H probably damaging Het
Ewsr1 A G 11: 5,041,450 (GRCm39) probably benign Het
Fn3k A T 11: 121,339,712 (GRCm39) N158I probably damaging Het
Galnt17 C T 5: 130,935,540 (GRCm39) R381Q probably damaging Het
Golga3 A T 5: 110,335,227 (GRCm39) K180N probably damaging Het
Hipk1 A G 3: 103,667,854 (GRCm39) L571P probably damaging Het
Hormad2 T A 11: 4,374,808 (GRCm39) K69* probably null Het
Hspg2 A T 4: 137,244,915 (GRCm39) D880V probably damaging Het
Ido2 T A 8: 25,030,652 (GRCm39) D226V probably damaging Het
Irs3 A G 5: 137,642,961 (GRCm39) F159S probably damaging Het
Itgb1bp1 T C 12: 21,326,860 (GRCm39) T38A probably damaging Het
Kif11 A G 19: 37,399,062 (GRCm39) I749V probably benign Het
Lrp1b T G 2: 40,769,567 (GRCm39) M2811L probably benign Het
Lysmd3 A G 13: 81,817,737 (GRCm39) Y238C probably damaging Het
Mapk11 G A 15: 89,030,575 (GRCm39) T106M probably damaging Het
Mdga2 C T 12: 66,736,155 (GRCm39) V358I possibly damaging Het
Muc4 C T 16: 32,754,563 (GRCm38) T1479I probably benign Het
Myom1 T C 17: 71,371,592 (GRCm39) V578A possibly damaging Het
Nek10 T G 14: 14,885,047 (GRCm38) probably null Het
Or2t47 T A 11: 58,442,768 (GRCm39) Q99L probably damaging Het
Or4k1 T A 14: 50,377,580 (GRCm39) N172I probably damaging Het
Or51a24 T G 7: 103,734,033 (GRCm39) T85P probably benign Het
Or51v14 G C 7: 103,261,443 (GRCm39) T39R possibly damaging Het
Or52a5b G T 7: 103,417,168 (GRCm39) C145* probably null Het
Or8d23 T C 9: 38,841,875 (GRCm39) M136T probably damaging Het
Pde4dip A T 3: 97,664,937 (GRCm39) C333S probably benign Het
Pglyrp2 T A 17: 32,637,222 (GRCm39) I269F probably benign Het
Plch1 A G 3: 63,628,655 (GRCm39) V536A probably benign Het
Popdc2 T A 16: 38,183,188 (GRCm39) L57Q probably damaging Het
Pramel14 T C 4: 143,720,885 (GRCm39) R19G possibly damaging Het
Riox1 A G 12: 83,997,709 (GRCm39) K82E probably benign Het
Rnasel G A 1: 153,630,647 (GRCm39) V388M probably damaging Het
Smc1b A G 15: 85,006,052 (GRCm39) probably benign Het
Snx25 A G 8: 46,494,444 (GRCm39) S814P probably damaging Het
Spta1 A T 1: 174,056,824 (GRCm39) H1859L probably benign Het
Strc T C 2: 121,196,343 (GRCm39) I1562V probably benign Het
Taar5 T A 10: 23,846,986 (GRCm39) I128N probably damaging Het
Ttc28 C T 5: 111,325,483 (GRCm39) probably benign Het
Vcan A T 13: 89,851,648 (GRCm39) M1104K possibly damaging Het
Vnn1 C T 10: 23,776,653 (GRCm39) Q335* probably null Het
Zic1 T C 9: 91,246,946 (GRCm39) D42G possibly damaging Het
Other mutations in Nid2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01783:Nid2 APN 14 19,818,745 (GRCm39) missense probably benign
IGL01788:Nid2 APN 14 19,858,047 (GRCm39) missense probably damaging 1.00
IGL02259:Nid2 APN 14 19,818,277 (GRCm39) critical splice acceptor site probably null
IGL03084:Nid2 APN 14 19,819,000 (GRCm39) missense probably benign 0.05
IGL03247:Nid2 APN 14 19,829,688 (GRCm39) missense probably damaging 1.00
IGL03098:Nid2 UTSW 14 19,856,006 (GRCm39) missense probably damaging 0.99
PIT4810001:Nid2 UTSW 14 19,860,158 (GRCm39) missense possibly damaging 0.82
R0173:Nid2 UTSW 14 19,852,400 (GRCm39) splice site probably benign
R0501:Nid2 UTSW 14 19,839,736 (GRCm39) splice site probably null
R1117:Nid2 UTSW 14 19,813,732 (GRCm39) critical splice acceptor site probably null
R1305:Nid2 UTSW 14 19,818,930 (GRCm39) missense probably benign 0.00
R1572:Nid2 UTSW 14 19,855,480 (GRCm39) missense probably benign 0.08
R1594:Nid2 UTSW 14 19,831,329 (GRCm39) missense probably benign 0.03
R1789:Nid2 UTSW 14 19,802,499 (GRCm39) missense possibly damaging 0.95
R1927:Nid2 UTSW 14 19,818,344 (GRCm39) missense probably damaging 1.00
R2085:Nid2 UTSW 14 19,828,111 (GRCm39) missense probably benign 0.12
R2086:Nid2 UTSW 14 19,828,111 (GRCm39) missense probably benign 0.12
R2100:Nid2 UTSW 14 19,828,946 (GRCm39) nonsense probably null
R2240:Nid2 UTSW 14 19,855,982 (GRCm39) missense probably damaging 0.99
R2314:Nid2 UTSW 14 19,839,829 (GRCm39) missense probably benign 0.01
R2863:Nid2 UTSW 14 19,818,471 (GRCm39) missense possibly damaging 0.92
R3113:Nid2 UTSW 14 19,828,111 (GRCm39) missense probably benign 0.12
R3545:Nid2 UTSW 14 19,813,779 (GRCm39) missense probably damaging 1.00
R3548:Nid2 UTSW 14 19,813,779 (GRCm39) missense probably damaging 1.00
R3801:Nid2 UTSW 14 19,860,065 (GRCm39) missense probably damaging 1.00
R4618:Nid2 UTSW 14 19,858,078 (GRCm39) missense probably damaging 0.99
R4798:Nid2 UTSW 14 19,839,829 (GRCm39) missense probably benign 0.01
R4953:Nid2 UTSW 14 19,828,146 (GRCm39) nonsense probably null
R5256:Nid2 UTSW 14 19,818,276 (GRCm39) critical splice acceptor site probably null
R5289:Nid2 UTSW 14 19,855,379 (GRCm39) missense possibly damaging 0.95
R5302:Nid2 UTSW 14 19,829,769 (GRCm39) missense probably benign
R5409:Nid2 UTSW 14 19,856,030 (GRCm39) missense probably damaging 1.00
R5514:Nid2 UTSW 14 19,852,535 (GRCm39) missense probably damaging 1.00
R6134:Nid2 UTSW 14 19,828,851 (GRCm39) missense probably damaging 1.00
R6365:Nid2 UTSW 14 19,853,201 (GRCm39) missense probably damaging 1.00
R6647:Nid2 UTSW 14 19,852,484 (GRCm39) missense probably benign 0.04
R6758:Nid2 UTSW 14 19,852,551 (GRCm39) missense probably damaging 1.00
R6882:Nid2 UTSW 14 19,839,775 (GRCm39) missense probably damaging 1.00
R6893:Nid2 UTSW 14 19,839,855 (GRCm39) missense probably benign 0.34
R7045:Nid2 UTSW 14 19,829,749 (GRCm39) missense possibly damaging 0.94
R7392:Nid2 UTSW 14 19,818,724 (GRCm39) missense probably benign 0.00
R7477:Nid2 UTSW 14 19,856,041 (GRCm39) missense probably benign 0.09
R7515:Nid2 UTSW 14 19,841,635 (GRCm39) missense probably benign 0.06
R7547:Nid2 UTSW 14 19,847,345 (GRCm39) missense probably benign
R7594:Nid2 UTSW 14 19,818,791 (GRCm39) missense probably benign 0.00
R7615:Nid2 UTSW 14 19,852,598 (GRCm39) missense probably damaging 1.00
R7680:Nid2 UTSW 14 19,829,715 (GRCm39) missense probably damaging 1.00
R8097:Nid2 UTSW 14 19,848,657 (GRCm39) missense possibly damaging 0.77
R8167:Nid2 UTSW 14 19,860,131 (GRCm39) missense possibly damaging 0.57
R8292:Nid2 UTSW 14 19,818,346 (GRCm39) missense probably damaging 0.99
R8766:Nid2 UTSW 14 19,802,340 (GRCm39) missense probably benign 0.26
R9093:Nid2 UTSW 14 19,858,009 (GRCm39) missense
R9193:Nid2 UTSW 14 19,853,278 (GRCm39) missense probably damaging 1.00
RF002:Nid2 UTSW 14 19,801,434 (GRCm39) small deletion probably benign
RF016:Nid2 UTSW 14 19,801,431 (GRCm39) small deletion probably benign
X0009:Nid2 UTSW 14 19,852,579 (GRCm39) missense probably damaging 1.00
X0021:Nid2 UTSW 14 19,818,930 (GRCm39) missense probably benign 0.00
X0026:Nid2 UTSW 14 19,828,199 (GRCm39) missense probably damaging 0.96
Z1177:Nid2 UTSW 14 19,839,876 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGGCTGAATGTTATTCCCATCTTTG -3'
(R):5'- TGCTGGGATTCAGGCATCAG -3'

Sequencing Primer
(F):5'- GAATGTTATTCCCATCTTTGTAGCG -3'
(R):5'- CTGGGATTCAGGCATCAGAGATG -3'
Posted On 2014-10-01