Incidental Mutation 'R2158:Pglyrp2'
ID235017
Institutional Source Beutler Lab
Gene Symbol Pglyrp2
Ensembl Gene ENSMUSG00000079563
Gene Namepeptidoglycan recognition protein 2
SynonymstagL-alpha, PGRP-L, tagl-beta, tagL, C730002N09Rik, Pglyrpl
MMRRC Submission 040161-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R2158 (G1)
Quality Score225
Status Validated
Chromosome17
Chromosomal Location32413100-32424167 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 32418248 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 269 (I269F)
Ref Sequence ENSEMBL: ENSMUSP00000129964 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000114455] [ENSMUST00000170392]
Predicted Effect probably benign
Transcript: ENSMUST00000114455
AA Change: I269F

PolyPhen 2 Score 0.122 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000110099
Gene: ENSMUSG00000079563
AA Change: I269F

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
low complexity region 251 266 N/A INTRINSIC
low complexity region 270 281 N/A INTRINSIC
PGRP 360 506 6.61e-78 SMART
Ami_2 373 512 6.28e-10 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000170392
AA Change: I269F

PolyPhen 2 Score 0.122 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000129964
Gene: ENSMUSG00000079563
AA Change: I269F

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
low complexity region 251 266 N/A INTRINSIC
low complexity region 270 281 N/A INTRINSIC
PGRP 360 506 6.61e-78 SMART
Ami_2 373 512 6.28e-10 SMART
Meta Mutation Damage Score 0.1224 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.6%
  • 20x: 96.0%
Validation Efficiency 100% (59/59)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a peptidoglycan recognition protein, which belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. This protein hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in bacterial cell wall glycopeptides, and thus may play a scavenger role by digesting biologically active peptidoglycan into biologically inactive fragments. [provided by RefSeq, Sep 2011]
PHENOTYPE: Mice homozygous for disruption of this gene are viable and fertile with no gross developmental defects. Mice homozygous for a different knock-out allele are resistant to peptidoglycan- or muramyl dipeptide-induced arthritis and increased susceptibility to DSS-induced colitis. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1810055G02Rik T C 19: 3,716,608 V65A possibly damaging Het
4930402F06Rik A T 2: 35,385,668 S38T possibly damaging Het
Adam4 A G 12: 81,421,763 L28S probably damaging Het
Agmo T A 12: 37,357,710 F198I probably damaging Het
Akap7 A G 10: 25,171,164 V45A probably damaging Het
Amotl1 G T 9: 14,575,169 N476K probably benign Het
Apcs A G 1: 172,894,533 L82P probably damaging Het
Armc3 C A 2: 19,248,633 P195Q probably damaging Het
Astn2 A T 4: 66,404,254 L36Q unknown Het
Atad2 A T 15: 58,098,566 S870T possibly damaging Het
Bmp10 A T 6: 87,434,080 D285V probably benign Het
Caskin1 T C 17: 24,505,154 V972A probably benign Het
Ccdc141 T A 2: 77,030,671 N921Y probably damaging Het
Cntnap5b A T 1: 100,390,572 D1019V probably damaging Het
Eml5 T C 12: 98,843,946 probably benign Het
Evi5l T C 8: 4,193,195 Y360H probably damaging Het
Ewsr1 A G 11: 5,091,450 probably benign Het
Fn3k A T 11: 121,448,886 N158I probably damaging Het
Galnt17 C T 5: 130,906,702 R381Q probably damaging Het
Golga3 A T 5: 110,187,361 K180N probably damaging Het
Hipk1 A G 3: 103,760,538 L571P probably damaging Het
Hormad2 T A 11: 4,424,808 K69* probably null Het
Hspg2 A T 4: 137,517,604 D880V probably damaging Het
Ido2 T A 8: 24,540,636 D226V probably damaging Het
Irs3 A G 5: 137,644,699 F159S probably damaging Het
Itgb1bp1 T C 12: 21,276,859 T38A probably damaging Het
Kif11 A G 19: 37,410,614 I749V probably benign Het
Lrp1b T G 2: 40,879,555 M2811L probably benign Het
Lysmd3 A G 13: 81,669,618 Y238C probably damaging Het
Mapk11 G A 15: 89,146,372 T106M probably damaging Het
Mdga2 C T 12: 66,689,381 V358I possibly damaging Het
Muc4 C T 16: 32,754,563 T1479I probably benign Het
Myom1 T C 17: 71,064,597 V578A possibly damaging Het
Nek10 T G 14: 14,885,047 probably null Het
Nid2 G A 14: 19,778,043 G516S probably benign Het
Olfr328 T A 11: 58,551,942 Q99L probably damaging Het
Olfr620 G C 7: 103,612,236 T39R possibly damaging Het
Olfr645 T G 7: 104,084,826 T85P probably benign Het
Olfr69 G T 7: 103,767,961 C145* probably null Het
Olfr728 T A 14: 50,140,123 N172I probably damaging Het
Olfr930 T C 9: 38,930,579 M136T probably damaging Het
Pde4dip A T 3: 97,757,621 C333S probably benign Het
Plch1 A G 3: 63,721,234 V536A probably benign Het
Popdc2 T A 16: 38,362,826 L57Q probably damaging Het
Pramef17 T C 4: 143,994,315 R19G possibly damaging Het
Riox1 A G 12: 83,950,935 K82E probably benign Het
Rnasel G A 1: 153,754,901 V388M probably damaging Het
Smc1b A G 15: 85,121,851 probably benign Het
Snx25 A G 8: 46,041,407 S814P probably damaging Het
Spta1 A T 1: 174,229,258 H1859L probably benign Het
Strc T C 2: 121,365,862 I1562V probably benign Het
Taar5 T A 10: 23,971,088 I128N probably damaging Het
Ttc28 C T 5: 111,177,617 probably benign Het
Vcan A T 13: 89,703,529 M1104K possibly damaging Het
Vnn1 C T 10: 23,900,755 Q335* probably null Het
Zic1 T C 9: 91,364,893 D42G possibly damaging Het
Other mutations in Pglyrp2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01935:Pglyrp2 APN 17 32418577 missense probably benign 0.14
IGL01949:Pglyrp2 APN 17 32416106 splice site probably null
IGL02355:Pglyrp2 APN 17 32417022 missense probably damaging 1.00
IGL02362:Pglyrp2 APN 17 32417022 missense probably damaging 1.00
IGL02601:Pglyrp2 APN 17 32415861 missense probably benign 0.04
IGL02965:Pglyrp2 APN 17 32418586 missense probably benign 0.00
R0324:Pglyrp2 UTSW 17 32418328 missense probably benign 0.00
R0386:Pglyrp2 UTSW 17 32420862 start codon destroyed probably null 0.93
R2181:Pglyrp2 UTSW 17 32418962 missense probably damaging 1.00
R2191:Pglyrp2 UTSW 17 32415957 missense probably benign 0.04
R2313:Pglyrp2 UTSW 17 32418699 missense probably damaging 1.00
R4825:Pglyrp2 UTSW 17 32418261 missense probably benign 0.00
R4852:Pglyrp2 UTSW 17 32415849 missense probably benign 0.09
R4888:Pglyrp2 UTSW 17 32418797 missense probably benign 0.26
R6941:Pglyrp2 UTSW 17 32416074 missense probably damaging 1.00
R7014:Pglyrp2 UTSW 17 32415930 missense probably damaging 0.98
Predicted Primers PCR Primer
(F):5'- AACTGTAGGTGTTCTGGCTC -3'
(R):5'- ACTCCTGGCAATCACCTTGG -3'

Sequencing Primer
(F):5'- CTCCAGTTTCTGTAACAGGACAAGG -3'
(R):5'- GCTGGTGACTTAGGTCTGACC -3'
Posted On2014-10-01