Mutagenetix > Incidental Mutation

Incidental Mutation 'R2179:Tas2r121'

237046

Institutional Source

Beutler Lab

Gene Symbol

Tas2r121

Ensembl Gene

ENSMUSG00000071150

Gene Name

taste receptor, type 2, member 121

Synonyms

mGR21, T2R21, Tas2r21, mT2r48

MMRRC Submission

040181-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R2179 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

132677053-132677970 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 132677831 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 47 (I47N)

Ref Sequence

ENSEMBL: ENSMUSP00000093044 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095395]

AlphaFold

Q7M720

Predicted Effect

probably damaging
Transcript: ENSMUST00000095395
AA Change: I47N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000093044
Gene: ENSMUSG00000071150
AA Change: I47N

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	300	4.5e-108	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene product belongs to the family of candidate taste receptors that are members of the G-protein-coupled receptor superfamily. These proteins are specifically expressed in the taste receptor cells of the tongue and palate epithelia. They are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. In functional expression studies, they respond to bitter tastants. This gene maps to the taste receptor gene cluster on chromosome 12p13. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgb	T	C	10: 10,271,018 (GRCm39)	D849G	possibly damaging	Het
Amz2	A	G	11: 109,320,658 (GRCm39)	H155R	probably damaging	Het
Cfhr4	G	T	1: 139,659,279 (GRCm39)	P679Q	probably damaging	Het
Chuk	C	A	19: 44,092,160 (GRCm39)	C46F	possibly damaging	Het
Cp	A	T	3: 20,042,151 (GRCm39)	D973V	probably damaging	Het
Cpm	T	A	10: 117,519,266 (GRCm39)	D391E	probably benign	Het
Creb3	T	C	4: 43,566,306 (GRCm39)	S271P	probably damaging	Het
Cubn	T	A	2: 13,323,053 (GRCm39)	H2838L	possibly damaging	Het
Dennd2d	A	G	3: 106,399,776 (GRCm39)	H233R	probably benign	Het
Dnaaf2	C	A	12: 69,245,071 (GRCm39)		probably benign	Het
Enpp3	T	A	10: 24,681,793 (GRCm39)	Q304H	probably benign	Het
Fermt3	C	A	19: 6,991,782 (GRCm39)	R143L	probably benign	Het
Fndc8	A	C	11: 82,789,580 (GRCm39)	K246T	probably damaging	Het
Gaa	A	G	11: 119,165,884 (GRCm39)		probably null	Het
Gucy2e	A	T	11: 69,119,404 (GRCm39)		probably null	Het
Igf1r	A	G	7: 67,653,698 (GRCm39)	T79A	probably damaging	Het
Ikbkb	C	T	8: 23,171,769 (GRCm39)		probably null	Het
Il16	A	T	7: 83,337,287 (GRCm39)		probably null	Het
Irs1	T	A	1: 82,267,940 (GRCm39)	H92L	possibly damaging	Het
Itpr2	A	G	6: 146,277,464 (GRCm39)	M315T	probably benign	Het
Lrba	T	G	3: 86,261,588 (GRCm39)	L1514R	probably damaging	Het
Mcpt2	A	G	14: 56,279,573 (GRCm39)		probably benign	Het
Metap1d	A	G	2: 71,283,715 (GRCm39)	I5V	probably benign	Het
Mga	T	A	2: 119,790,923 (GRCm39)	S2479T	probably damaging	Het
Mllt10	T	C	2: 18,215,604 (GRCm39)	V1063A	probably damaging	Het
Mroh2b	G	A	15: 4,950,928 (GRCm39)		probably null	Het
Mtfr1	C	T	3: 19,254,308 (GRCm39)	R15*	probably null	Het
Npm2	A	G	14: 70,885,749 (GRCm39)	V152A	probably benign	Het
Nrxn3	T	A	12: 89,221,448 (GRCm39)	V409D	probably damaging	Het
Ogdhl	A	G	14: 32,057,302 (GRCm39)	N303D	probably damaging	Het
Or10g9	T	C	9: 39,912,220 (GRCm39)	Y101C	probably benign	Het
Or10q1b	T	C	19: 13,682,758 (GRCm39)	V189A	probably damaging	Het
Or4d11	T	C	19: 12,013,452 (GRCm39)	Y218C	probably damaging	Het
Or6s1	A	G	14: 51,308,238 (GRCm39)	V204A	probably benign	Het
Pah	T	C	10: 87,403,197 (GRCm39)	F191L	probably damaging	Het
Paics	A	T	5: 77,109,291 (GRCm39)	I209F	probably damaging	Het
Pcare	T	C	17: 72,059,521 (GRCm39)	D52G	probably damaging	Het
Pld6	A	G	11: 59,678,184 (GRCm39)	L93P	probably damaging	Het
Plod3	T	C	5: 137,019,862 (GRCm39)	F431L	possibly damaging	Het
Ppm1b	T	A	17: 85,301,862 (GRCm39)	D247E	probably damaging	Het
Prx	A	G	7: 27,217,410 (GRCm39)	D637G	probably benign	Het
Rsf1	GGCGGCGGC	GGCGGCGGCCGCGGCGGC	7: 97,229,116 (GRCm39)		probably benign	Het
Ryr2	A	T	13: 11,720,679 (GRCm39)	Y2656*	probably null	Het
Setd2	T	A	9: 110,423,756 (GRCm39)	Y492*	probably null	Het
Shmt1	A	G	11: 60,697,825 (GRCm39)	W9R	possibly damaging	Het
Slc25a17	A	G	15: 81,222,151 (GRCm39)	V107A	probably benign	Het
Sptlc1	A	T	13: 53,505,675 (GRCm39)	Y248N	probably damaging	Het
Stam2	T	C	2: 52,584,936 (GRCm39)	T453A	probably benign	Het
Tango2	G	T	16: 18,128,762 (GRCm39)	N77K	probably damaging	Het
Terb1	A	T	8: 105,179,347 (GRCm39)	C614S	probably damaging	Het
Terb1	T	C	8: 105,199,369 (GRCm39)	N525S	probably benign	Het
Ttc6	A	G	12: 57,719,904 (GRCm39)	D825G	possibly damaging	Het
U90926	A	T	5: 92,357,838 (GRCm39)	H104Q	probably benign	Het
Zfand4	G	A	6: 116,291,742 (GRCm39)	A559T	possibly damaging	Het
Zfp462	T	C	4: 55,009,524 (GRCm39)	S497P	possibly damaging	Het

Other mutations in Tas2r121

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00559:Tas2r121	APN	6	132,677,484 (GRCm39)	missense	probably benign	0.28
IGL01868:Tas2r121	APN	6	132,677,235 (GRCm39)	missense	probably benign	0.00
IGL02182:Tas2r121	APN	6	132,677,133 (GRCm39)	missense	probably damaging	0.99
IGL02728:Tas2r121	APN	6	132,677,480 (GRCm39)	missense	probably damaging	1.00
R0833:Tas2r121	UTSW	6	132,677,325 (GRCm39)	missense	probably damaging	1.00
R0836:Tas2r121	UTSW	6	132,677,325 (GRCm39)	missense	probably damaging	1.00
R1181:Tas2r121	UTSW	6	132,677,132 (GRCm39)	missense	probably damaging	1.00
R1424:Tas2r121	UTSW	6	132,677,645 (GRCm39)	missense	probably damaging	1.00
R1583:Tas2r121	UTSW	6	132,677,193 (GRCm39)	nonsense	probably null
R4711:Tas2r121	UTSW	6	132,677,853 (GRCm39)	missense	probably benign	0.28
R5274:Tas2r121	UTSW	6	132,677,811 (GRCm39)	missense	probably damaging	1.00
R5308:Tas2r121	UTSW	6	132,677,480 (GRCm39)	missense	possibly damaging	0.84
R5663:Tas2r121	UTSW	6	132,677,520 (GRCm39)	missense	probably benign	0.10
R5668:Tas2r121	UTSW	6	132,677,756 (GRCm39)	missense	possibly damaging	0.95
R5885:Tas2r121	UTSW	6	132,677,254 (GRCm39)	missense	probably damaging	1.00
R6395:Tas2r121	UTSW	6	132,677,495 (GRCm39)	missense	probably benign	0.23
R7552:Tas2r121	UTSW	6	132,677,505 (GRCm39)	missense	probably benign	0.17
R8094:Tas2r121	UTSW	6	132,677,772 (GRCm39)	missense	probably benign
R9192:Tas2r121	UTSW	6	132,677,492 (GRCm39)	missense	probably benign	0.00
R9718:Tas2r121	UTSW	6	132,677,765 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGGAGAAACTGGCTATTTTGAGC -3'
(R):5'- ACAGGGACATTTCACCTTGCC -3'

Sequencing Primer
(F):5'- GGCTATTTTGAGCAAATAAAAGATGC -3'
(R):5'- GGGACATTTCACCTTGCCAAATGAG -3'

Posted On

2014-10-02