Mutagenetix > Incidental Mutation

Incidental Mutation 'R2180:Or10x1'

237097

Institutional Source

Beutler Lab

Gene Symbol

Or10x1

Ensembl Gene

ENSMUSG00000066672

Gene Name

olfactory receptor family 10 subfamily X member 1

Synonyms

Olfr417, MOR267-11, GA_x6K02T2P20D-20787051-20786119

MMRRC Submission

040182-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R2180 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

174196485-174197414 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 174196967 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 161 (I161M)

Ref Sequence

ENSEMBL: ENSMUSP00000151947 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085862] [ENSMUST00000217962] [ENSMUST00000220394]

AlphaFold

F8VQB1

Predicted Effect

probably damaging
Transcript: ENSMUST00000085862
AA Change: I161M

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000083024
Gene: ENSMUSG00000066672
AA Change: I161M

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	5.7e-46	PFAM
Pfam:7tm_1	39	303	6.6e-20	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000217962
AA Change: I161M

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000220394
AA Change: I161M

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts5	T	C	16: 85,684,812 (GRCm39)	D377G	probably damaging	Het
Adgrl4	A	T	3: 151,205,779 (GRCm39)	I164F	probably damaging	Het
Anxa9	T	C	3: 95,213,735 (GRCm39)		probably null	Het
Aox4	A	T	1: 58,252,226 (GRCm39)	T34S	probably benign	Het
Asic1	G	T	15: 99,569,846 (GRCm39)	V56F	probably benign	Het
Atpaf1	T	C	4: 115,645,557 (GRCm39)	M1T	probably null	Het
Axin1	A	G	17: 26,362,309 (GRCm39)	T218A	probably benign	Het
Bdp1	ATTCTTCTTCTTCTTCTTC	ATTCTTCTTCTTCTTCTTCTTC	13: 100,197,913 (GRCm39)		probably benign	Het
Birc6	T	C	17: 74,919,146 (GRCm39)	I1988T	probably benign	Het
Btbd7	A	T	12: 102,752,156 (GRCm39)	D869E	probably damaging	Het
Caln1	T	C	5: 130,868,249 (GRCm39)	*220Q	probably null	Het
Ccdc150	G	A	1: 54,311,706 (GRCm39)		probably null	Het
Ccnt1	A	T	15: 98,441,481 (GRCm39)	S596T	possibly damaging	Het
Cd44	C	T	2: 102,658,955 (GRCm39)	G640E	possibly damaging	Het
Cep192	A	G	18: 67,957,813 (GRCm39)	E582G	possibly damaging	Het
Clcnkb	G	T	4: 141,136,819 (GRCm39)		probably null	Het
Dhx29	T	C	13: 113,099,406 (GRCm39)		probably null	Het
Dnah8	T	C	17: 31,059,621 (GRCm39)	F4407S	probably benign	Het
Enah	A	T	1: 181,746,024 (GRCm39)	M419K	probably damaging	Het
Fancd2	A	T	6: 113,551,598 (GRCm39)	T1055S	probably benign	Het
Gigyf2	G	A	1: 87,344,642 (GRCm39)	G525D	probably damaging	Het
Gm5800	T	A	14: 51,953,451 (GRCm39)	K55*	probably null	Het
Gpr149	A	G	3: 62,511,489 (GRCm39)	L170P	probably damaging	Het
Grik4	A	T	9: 42,453,301 (GRCm39)	Y695N	probably benign	Het
Gsg1	A	T	6: 135,217,143 (GRCm39)	V228D	probably damaging	Het
Helb	G	A	10: 119,941,353 (GRCm39)	T445M	probably benign	Het
Helz2	A	T	2: 180,875,525 (GRCm39)	D1656E	probably damaging	Het
Hyou1	A	T	9: 44,299,316 (GRCm39)	K669M	probably benign	Het
Itga2	T	C	13: 114,985,917 (GRCm39)	N953D	possibly damaging	Het
Ldhd	T	C	8: 112,356,018 (GRCm39)	I122V	probably benign	Het
Lrrtm1	A	G	6: 77,221,329 (GRCm39)	D262G	probably damaging	Het
Mapkapk5	T	C	5: 121,673,927 (GRCm39)		probably null	Het
Msantd5l	C	A	11: 51,145,437 (GRCm39)	W50L	probably damaging	Het
Niban1	A	T	1: 151,593,829 (GRCm39)	H838L	probably benign	Het
Numa1	T	C	7: 101,649,197 (GRCm39)	I976T	probably benign	Het
Or2a12	C	T	6: 42,904,459 (GRCm39)	T98I	probably benign	Het
Or4f14b	A	G	2: 111,775,348 (GRCm39)	V151A	probably benign	Het
Or52e4	T	C	7: 104,706,092 (GRCm39)	I213T	probably benign	Het
Patj	G	A	4: 98,411,739 (GRCm39)		probably null	Het
Pfas	T	C	11: 68,883,013 (GRCm39)	D757G	possibly damaging	Het
Pom121l2	A	G	13: 22,166,145 (GRCm39)	N139D	probably benign	Het
Ppp2r3d	A	T	9: 101,004,214 (GRCm39)	Y994*	probably null	Het
Ppp6c	T	C	2: 39,087,525 (GRCm39)	D227G	probably benign	Het
Ptpn13	T	G	5: 103,717,424 (GRCm39)	H1855Q	probably damaging	Het
Ptprh	T	A	7: 4,604,867 (GRCm39)	Q59L	probably benign	Het
Rap1gap2	T	C	11: 74,283,972 (GRCm39)	K669E	probably benign	Het
Rbl2	T	C	8: 91,816,683 (GRCm39)	S348P	possibly damaging	Het
Rptor	T	A	11: 119,615,970 (GRCm39)	N161K	probably damaging	Het
Satb1	C	T	17: 52,110,524 (GRCm39)	A192T	probably damaging	Het
Scn5a	A	G	9: 119,345,117 (GRCm39)	V1083A	probably benign	Het
Sec14l2	C	T	11: 4,058,964 (GRCm39)	A194T	probably damaging	Het
Sema4b	A	G	7: 79,862,583 (GRCm39)	N53S	probably benign	Het
Sin3b	T	A	8: 73,479,923 (GRCm39)	Y876*	probably null	Het
Smchd1	C	A	17: 71,770,794 (GRCm39)	M129I	probably benign	Het
Spmip6	T	A	4: 41,507,170 (GRCm39)	M209L	probably benign	Het
Tmc1	T	C	19: 20,801,448 (GRCm39)	Y484C	probably damaging	Het
Utp20	A	G	10: 88,656,801 (GRCm39)	S135P	probably damaging	Het
Zfp266	A	T	9: 20,410,975 (GRCm39)	C401S	probably damaging	Het
Zfp738	G	A	13: 67,819,313 (GRCm39)	T226I	probably damaging	Het
Zfp871	G	A	17: 32,994,275 (GRCm39)	T300M	probably damaging	Het

Other mutations in Or10x1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01885:Or10x1	APN	1	174,196,967 (GRCm39)	missense	probably damaging	0.99
PIT4366001:Or10x1	UTSW	1	174,196,656 (GRCm39)	missense	probably damaging	1.00
R0131:Or10x1	UTSW	1	174,197,152 (GRCm39)	missense	probably damaging	0.99
R0131:Or10x1	UTSW	1	174,197,152 (GRCm39)	missense	probably damaging	0.99
R0132:Or10x1	UTSW	1	174,197,152 (GRCm39)	missense	probably damaging	0.99
R1623:Or10x1	UTSW	1	174,196,515 (GRCm39)	missense	probably benign	0.43
R1862:Or10x1	UTSW	1	174,197,018 (GRCm39)	missense	probably damaging	0.97
R1901:Or10x1	UTSW	1	174,196,734 (GRCm39)	missense	probably benign
R1972:Or10x1	UTSW	1	174,197,136 (GRCm39)	missense	probably benign	0.05
R4413:Or10x1	UTSW	1	174,197,040 (GRCm39)	missense	probably damaging	0.98
R4528:Or10x1	UTSW	1	174,196,822 (GRCm39)	missense	probably damaging	1.00
R4750:Or10x1	UTSW	1	174,196,488 (GRCm39)	missense	probably benign	0.02
R4849:Or10x1	UTSW	1	174,196,966 (GRCm39)	missense	probably damaging	0.97
R4851:Or10x1	UTSW	1	174,196,562 (GRCm39)	missense	probably benign	0.44
R5394:Or10x1	UTSW	1	174,196,836 (GRCm39)	missense	probably damaging	1.00
R5916:Or10x1	UTSW	1	174,196,698 (GRCm39)	missense	probably damaging	1.00
R6248:Or10x1	UTSW	1	174,197,236 (GRCm39)	missense	probably benign
R7311:Or10x1	UTSW	1	174,196,759 (GRCm39)	missense	probably benign	0.30
R7868:Or10x1	UTSW	1	174,196,551 (GRCm39)	missense	probably benign	0.00
Z1088:Or10x1	UTSW	1	174,197,310 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CAGCCGCATTTCAGTCACAG -3'
(R):5'- CAGCTGAAGGGATCTTGAGG -3'

Sequencing Primer
(F):5'- CAGATGTGTTTCTTCCTGGGCC -3'
(R):5'- CTGAAGGGATCTTGAGGACAGAAG -3'

Posted On

2014-10-02