Mutagenetix > Incidental Mutation

Incidental Mutation 'R2192:Hectd4'

238197

Institutional Source

Beutler Lab

Gene Symbol

Hectd4

Ensembl Gene

ENSMUSG00000042744

Gene Name

HECT domain E3 ubiquitin protein ligase 4

Synonyms

Gm15800

MMRRC Submission

040194-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.927) question?

Stock #

R2192 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

121358282-121506640 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 121453206 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 571 (V571A)

Ref Sequence

ENSEMBL: ENSMUSP00000098332 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042614] [ENSMUST00000100769]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000042614
AA Change: V1908A

PolyPhen 2 Score 0.326 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000048345
Gene: ENSMUSG00000042744
AA Change: V1908A

Domain	Start	End	E-Value	Type
low complexity region	224	234	N/A	INTRINSIC
low complexity region	266	282	N/A	INTRINSIC
low complexity region	553	564	N/A	INTRINSIC
low complexity region	725	735	N/A	INTRINSIC
low complexity region	1252	1265	N/A	INTRINSIC
coiled coil region	1372	1398	N/A	INTRINSIC
low complexity region	1551	1562	N/A	INTRINSIC
low complexity region	1725	1741	N/A	INTRINSIC
low complexity region	1892	1904	N/A	INTRINSIC
low complexity region	2656	2666	N/A	INTRINSIC
low complexity region	2857	2872	N/A	INTRINSIC
low complexity region	2901	2917	N/A	INTRINSIC
low complexity region	2921	2933	N/A	INTRINSIC
low complexity region	3232	3246	N/A	INTRINSIC
low complexity region	3275	3335	N/A	INTRINSIC
low complexity region	3441	3448	N/A	INTRINSIC
low complexity region	3473	3506	N/A	INTRINSIC
low complexity region	3512	3533	N/A	INTRINSIC
low complexity region	3540	3554	N/A	INTRINSIC
low complexity region	3794	3822	N/A	INTRINSIC
HECTc	4048	4412	4.78e-11	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000100769
AA Change: V571A

PolyPhen 2 Score 0.455 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000098332
Gene: ENSMUSG00000042744
AA Change: V571A

Domain	Start	End	E-Value	Type
coiled coil region	35	61	N/A	INTRINSIC
low complexity region	214	225	N/A	INTRINSIC
low complexity region	388	404	N/A	INTRINSIC
low complexity region	555	567	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130655

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155743

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adra1d	A	T	2: 131,403,289 (GRCm39)	M267K	probably damaging	Het
Aen	T	C	7: 78,555,793 (GRCm39)		probably null	Het
Ano2	A	G	6: 125,992,502 (GRCm39)	D825G	probably damaging	Het
Bdh2	T	C	3: 135,001,025 (GRCm39)	S142P	probably damaging	Het
Celsr1	A	G	15: 85,800,924 (GRCm39)	L2507P	possibly damaging	Het
Ces3a	T	C	8: 105,782,212 (GRCm39)	F308S	probably benign	Het
Cfap47	C	T	X: 78,454,218 (GRCm39)	R1646Q	probably damaging	Het
Chd4	T	A	6: 125,082,320 (GRCm39)	M603K	probably damaging	Het
Cit	T	C	5: 116,106,068 (GRCm39)	V984A	probably benign	Het
Clstn3	A	T	6: 124,436,166 (GRCm39)	D194E	probably damaging	Het
Ctdp1	T	C	18: 80,492,696 (GRCm39)	R600G	probably benign	Het
Ctnnd1	C	T	2: 84,439,907 (GRCm39)	G801D	probably damaging	Het
Dgkg	A	G	16: 22,407,049 (GRCm39)	F179L	probably damaging	Het
Dlg3	G	A	X: 99,817,827 (GRCm39)	D379N	probably damaging	Het
Drd2	G	T	9: 49,314,571 (GRCm39)	R267S	probably benign	Het
Dsg1b	T	C	18: 20,542,675 (GRCm39)	*1061Q	probably null	Het
Dvl1	G	A	4: 155,932,273 (GRCm39)	V28I	possibly damaging	Het
Edc3	T	C	9: 57,620,826 (GRCm39)	V49A	probably damaging	Het
Ern1	G	A	11: 106,300,750 (GRCm39)	T548I	probably benign	Het
Fam186a	T	C	15: 99,838,192 (GRCm39)	D2684G	possibly damaging	Het
Fam20a	A	G	11: 109,565,449 (GRCm39)	M454T	probably benign	Het
Fbp2	A	G	13: 63,006,056 (GRCm39)	M19T	possibly damaging	Het
Frmd4b	A	T	6: 97,464,577 (GRCm39)	C47S	probably damaging	Het
Gtf2h1	T	A	7: 46,464,747 (GRCm39)	I388N	possibly damaging	Het
Helz2	T	A	2: 180,870,841 (GRCm39)	K2832*	probably null	Het
Herc1	T	A	9: 66,372,688 (GRCm39)	D3081E	probably damaging	Het
Hmcn1	A	T	1: 150,591,566 (GRCm39)	S1878T	probably damaging	Het
Iars1	A	G	13: 49,841,605 (GRCm39)		probably null	Het
Il17rd	A	T	14: 26,816,835 (GRCm39)	K180M	probably damaging	Het
Ints6l	T	A	X: 55,550,110 (GRCm39)	H678Q	probably benign	Het
Khdrbs1	A	G	4: 129,613,830 (GRCm39)		probably null	Het
Lce3a	A	C	3: 92,832,837 (GRCm39)	S88A	unknown	Het
Lgals12	C	T	19: 7,578,606 (GRCm39)		probably null	Het
Lnpk	T	C	2: 74,399,373 (GRCm39)	T57A	probably benign	Het
Lrp2bp	C	T	8: 46,466,206 (GRCm39)	T105I	probably benign	Het
Lrrc4c	C	G	2: 97,459,657 (GRCm39)	N94K	possibly damaging	Het
Mag	A	T	7: 30,600,066 (GRCm39)	Y571*	probably null	Het
Mbd1	T	A	18: 74,410,449 (GRCm39)	D583E	probably damaging	Het
Mill1	T	A	7: 17,998,544 (GRCm39)	Y251*	probably null	Het
Mllt10	T	C	2: 18,211,871 (GRCm39)	I928T	probably benign	Het
Ms4a18	T	C	19: 10,991,029 (GRCm39)	T22A	probably benign	Het
Mtmr14	T	C	6: 113,257,700 (GRCm39)	F250L	probably damaging	Het
Mtmr3	A	T	11: 4,449,032 (GRCm39)	W244R	probably damaging	Het
Myorg	A	G	4: 41,497,704 (GRCm39)	I642T	probably damaging	Het
Nat10	C	A	2: 103,556,522 (GRCm39)	E885D	probably benign	Het
Nbeal1	T	A	1: 60,321,054 (GRCm39)	L2055H	probably damaging	Het
Necab3	T	A	2: 154,388,999 (GRCm39)	I192F	possibly damaging	Het
Nedd4	T	A	9: 72,650,000 (GRCm39)	N783K	probably damaging	Het
Nf2	A	T	11: 4,749,899 (GRCm39)	W61R	probably damaging	Het
Nox4	T	A	7: 87,023,588 (GRCm39)	F491L	probably benign	Het
Omt2b	G	A	9: 78,235,457 (GRCm39)		probably benign	Het
Or4c125	A	T	2: 89,170,009 (GRCm39)	Y212*	probably null	Het
Or4c127	T	A	2: 89,832,774 (GRCm39)	I8N	probably damaging	Het
Or8i2	A	G	2: 86,852,855 (GRCm39)	V11A	probably benign	Het
Osbpl5	A	T	7: 143,247,596 (GRCm39)	Y723*	probably null	Het
Otop2	T	C	11: 115,217,757 (GRCm39)	S198P	possibly damaging	Het
Pgam5	T	G	5: 110,413,785 (GRCm39)	H126P	probably damaging	Het
Phip	A	T	9: 82,753,868 (GRCm39)	N1625K	probably damaging	Het
Phospho2	T	A	2: 69,626,451 (GRCm39)	Y202*	probably null	Het
Pkd1l3	T	A	8: 110,347,156 (GRCm39)	H176Q	unknown	Het
Plxdc2	A	G	2: 16,570,147 (GRCm39)	R109G	probably damaging	Het
Prorsd1	T	C	11: 29,463,592 (GRCm39)	K57E	probably benign	Het
Rnf150	A	T	8: 83,591,020 (GRCm39)	R128W	probably damaging	Het
Samm50	G	A	15: 84,084,625 (GRCm39)		probably null	Het
Skint6	A	T	4: 112,722,909 (GRCm39)	Y889*	probably null	Het
Slc35b1	T	C	11: 95,276,640 (GRCm39)	Y40H	probably damaging	Het
Speg	A	G	1: 75,394,371 (GRCm39)	H1722R	probably damaging	Het
Suz12	T	C	11: 79,913,024 (GRCm39)	L356P	probably damaging	Het
Tln2	T	G	9: 67,262,503 (GRCm39)	I585L	probably damaging	Het
Trim2	G	A	3: 84,098,225 (GRCm39)	Q359*	probably null	Het
Ttc28	T	C	5: 111,371,362 (GRCm39)	Y635H	probably damaging	Het
Ttn	C	T	2: 76,543,549 (GRCm39)	E33146K	probably damaging	Het
Vcp	G	T	4: 42,982,547 (GRCm39)	T715K	probably benign	Het
Vmn1r230	T	A	17: 21,067,355 (GRCm39)	N181K	probably benign	Het
Vmn2r17	T	A	5: 109,582,144 (GRCm39)	M511K	possibly damaging	Het
Wdfy3	C	T	5: 102,055,408 (GRCm39)	R1554Q	possibly damaging	Het
Zfp112	A	T	7: 23,824,863 (GRCm39)	Q277L	probably damaging	Het
Zfp850	T	C	7: 27,684,620 (GRCm39)	E42G	probably damaging	Het
Zfp976	G	A	7: 42,262,695 (GRCm39)	P381S	probably damaging	Het
Zzef1	A	T	11: 72,800,982 (GRCm39)		probably null	Het

Other mutations in Hectd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Hectd4	APN	5	121,501,933 (GRCm39)	missense	possibly damaging	0.51
IGL00976:Hectd4	APN	5	121,487,169 (GRCm39)	missense	probably benign	0.18
IGL01085:Hectd4	APN	5	121,469,764 (GRCm39)	missense	probably damaging	1.00
IGL01112:Hectd4	APN	5	121,445,013 (GRCm39)	missense	probably benign	0.01
IGL01402:Hectd4	APN	5	121,477,480 (GRCm39)	splice site	probably benign
IGL01474:Hectd4	APN	5	121,474,712 (GRCm39)	missense	possibly damaging	0.53
IGL01503:Hectd4	APN	5	121,456,714 (GRCm39)	missense	probably benign	0.28
IGL01548:Hectd4	APN	5	121,502,723 (GRCm39)	missense	possibly damaging	0.71
IGL01656:Hectd4	APN	5	121,460,763 (GRCm39)	missense	probably damaging	0.99
IGL01756:Hectd4	APN	5	121,482,887 (GRCm39)	missense	probably benign	0.28
IGL01819:Hectd4	APN	5	121,466,481 (GRCm39)	missense	possibly damaging	0.85
IGL02080:Hectd4	APN	5	121,504,669 (GRCm39)	utr 3 prime	probably benign
IGL02488:Hectd4	APN	5	121,430,150 (GRCm39)	missense	probably benign	0.33
IGL02490:Hectd4	APN	5	121,456,676 (GRCm39)	missense	possibly damaging	0.82
IGL02558:Hectd4	APN	5	121,482,848 (GRCm39)	missense	probably benign	0.28
IGL02626:Hectd4	APN	5	121,491,944 (GRCm39)	missense	possibly damaging	0.86
IGL02649:Hectd4	APN	5	121,487,465 (GRCm39)	missense	possibly damaging	0.73
IGL02736:Hectd4	APN	5	121,480,782 (GRCm39)	missense	possibly damaging	0.73
IGL02861:Hectd4	APN	5	121,445,067 (GRCm39)	missense	possibly damaging	0.81
IGL02880:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02889:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02953:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02969:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03031:Hectd4	APN	5	121,486,857 (GRCm39)	missense	possibly damaging	0.96
IGL03066:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03160:Hectd4	APN	5	121,397,942 (GRCm39)	missense	probably benign
IGL03181:Hectd4	APN	5	121,492,021 (GRCm39)	missense	possibly damaging	0.91
IGL03265:Hectd4	APN	5	121,398,002 (GRCm39)	splice site	probably benign
IGL03375:Hectd4	APN	5	121,466,445 (GRCm39)	missense	possibly damaging	0.72
Achilles	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
agamemnon	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
clymnestra	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
hector	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
helen	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
Merriwether	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
PIT4466001:Hectd4	UTSW	5	121,471,123 (GRCm39)	critical splice donor site	probably null
R0018:Hectd4	UTSW	5	121,392,242 (GRCm39)	missense	possibly damaging	0.53
R0024:Hectd4	UTSW	5	121,446,639 (GRCm39)	missense	possibly damaging	0.92
R0030:Hectd4	UTSW	5	121,400,651 (GRCm39)	nonsense	probably null
R0080:Hectd4	UTSW	5	121,487,435 (GRCm39)	missense	probably benign	0.18
R0110:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0110:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0115:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0128:Hectd4	UTSW	5	121,487,306 (GRCm39)	missense	possibly damaging	0.86
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0132:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0244:Hectd4	UTSW	5	121,467,668 (GRCm39)	missense	probably benign	0.33
R0281:Hectd4	UTSW	5	121,392,314 (GRCm39)	missense	possibly damaging	0.85
R0329:Hectd4	UTSW	5	121,397,927 (GRCm39)	missense	probably benign
R0410:Hectd4	UTSW	5	121,424,329 (GRCm39)	missense	possibly damaging	0.86
R0422:Hectd4	UTSW	5	121,481,145 (GRCm39)	splice site	probably null
R0442:Hectd4	UTSW	5	121,462,045 (GRCm39)	missense	possibly damaging	0.66
R0449:Hectd4	UTSW	5	121,502,653 (GRCm39)	splice site	probably null
R0469:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0469:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0481:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0510:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0510:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0520:Hectd4	UTSW	5	121,469,770 (GRCm39)	missense	possibly damaging	0.53
R0534:Hectd4	UTSW	5	121,486,539 (GRCm39)	missense	possibly damaging	0.96
R0603:Hectd4	UTSW	5	121,442,400 (GRCm39)	missense	possibly damaging	0.46
R0617:Hectd4	UTSW	5	121,481,295 (GRCm39)	splice site	probably benign
R0622:Hectd4	UTSW	5	121,486,688 (GRCm39)	missense	possibly damaging	0.53
R0626:Hectd4	UTSW	5	121,415,887 (GRCm39)	missense	probably benign	0.18
R0708:Hectd4	UTSW	5	121,424,526 (GRCm39)	critical splice donor site	probably null
R0710:Hectd4	UTSW	5	121,474,691 (GRCm39)	missense	probably benign	0.08
R0763:Hectd4	UTSW	5	121,445,096 (GRCm39)	unclassified	probably benign
R0764:Hectd4	UTSW	5	121,424,832 (GRCm39)	missense	possibly damaging	0.46
R1123:Hectd4	UTSW	5	121,424,799 (GRCm39)	missense	probably damaging	0.96
R1129:Hectd4	UTSW	5	121,448,662 (GRCm39)	missense	possibly damaging	0.66
R1204:Hectd4	UTSW	5	121,488,548 (GRCm39)	missense	possibly damaging	0.85
R1237:Hectd4	UTSW	5	121,459,570 (GRCm39)	missense	possibly damaging	0.90
R1257:Hectd4	UTSW	5	121,456,687 (GRCm39)	nonsense	probably null
R1391:Hectd4	UTSW	5	121,491,758 (GRCm39)	missense	possibly damaging	0.96
R1395:Hectd4	UTSW	5	121,466,576 (GRCm39)	critical splice donor site	probably null
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1545:Hectd4	UTSW	5	121,462,019 (GRCm39)	missense	possibly damaging	0.87
R1553:Hectd4	UTSW	5	121,487,322 (GRCm39)	missense	probably benign	0.00
R1572:Hectd4	UTSW	5	121,439,941 (GRCm39)	missense	possibly damaging	0.85
R1662:Hectd4	UTSW	5	121,455,308 (GRCm39)	missense	probably benign	0.01
R1705:Hectd4	UTSW	5	121,436,167 (GRCm39)	missense	probably benign
R1715:Hectd4	UTSW	5	121,482,881 (GRCm39)	missense	possibly damaging	0.85
R1728:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1736:Hectd4	UTSW	5	121,487,593 (GRCm39)	missense	possibly damaging	0.53
R1768:Hectd4	UTSW	5	121,496,366 (GRCm39)	missense	possibly damaging	0.70
R1775:Hectd4	UTSW	5	121,429,254 (GRCm39)	splice site	probably benign
R1784:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1843:Hectd4	UTSW	5	121,435,243 (GRCm39)	missense	possibly damaging	0.53
R1914:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R1915:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R2024:Hectd4	UTSW	5	121,419,981 (GRCm39)	missense	possibly damaging	0.86
R2103:Hectd4	UTSW	5	121,493,692 (GRCm39)	missense	probably benign	0.04
R2108:Hectd4	UTSW	5	121,471,487 (GRCm39)	missense	possibly damaging	0.72
R2124:Hectd4	UTSW	5	121,456,702 (GRCm39)	missense	probably damaging	0.97
R2150:Hectd4	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
R2301:Hectd4	UTSW	5	121,491,600 (GRCm39)	missense	probably benign	0.18
R2324:Hectd4	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
R2331:Hectd4	UTSW	5	121,458,089 (GRCm39)	missense	probably benign	0.05
R2504:Hectd4	UTSW	5	121,402,030 (GRCm39)	missense	possibly damaging	0.73
R2504:Hectd4	UTSW	5	121,358,683 (GRCm39)	missense	unknown
R2904:Hectd4	UTSW	5	121,430,787 (GRCm39)	splice site	probably benign
R3843:Hectd4	UTSW	5	121,397,936 (GRCm39)	missense	possibly damaging	0.72
R3934:Hectd4	UTSW	5	121,458,164 (GRCm39)	critical splice donor site	probably null
R3944:Hectd4	UTSW	5	121,441,588 (GRCm39)	splice site	probably benign
R4133:Hectd4	UTSW	5	121,415,897 (GRCm39)	critical splice donor site	probably null
R4271:Hectd4	UTSW	5	121,358,567 (GRCm39)	small deletion	probably benign
R4413:Hectd4	UTSW	5	121,488,544 (GRCm39)	missense	possibly damaging	0.53
R4456:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R4489:Hectd4	UTSW	5	121,424,320 (GRCm39)	missense	possibly damaging	0.73
R4539:Hectd4	UTSW	5	121,452,970 (GRCm39)	nonsense	probably null
R4564:Hectd4	UTSW	5	121,488,494 (GRCm39)	missense	probably benign	0.33
R4582:Hectd4	UTSW	5	121,424,482 (GRCm39)	missense	possibly damaging	0.53
R4629:Hectd4	UTSW	5	121,435,266 (GRCm39)	missense	probably benign	0.01
R4633:Hectd4	UTSW	5	121,487,279 (GRCm39)	missense	probably benign	0.33
R4643:Hectd4	UTSW	5	121,487,118 (GRCm39)	missense	possibly damaging	0.53
R4679:Hectd4	UTSW	5	121,463,314 (GRCm39)	missense	possibly damaging	0.72
R4681:Hectd4	UTSW	5	121,441,678 (GRCm39)	missense	possibly damaging	0.86
R4734:Hectd4	UTSW	5	121,480,040 (GRCm39)	missense	possibly damaging	0.53
R4739:Hectd4	UTSW	5	121,486,505 (GRCm39)	missense	probably benign
R4781:Hectd4	UTSW	5	121,444,170 (GRCm39)	critical splice donor site	probably null
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4869:Hectd4	UTSW	5	121,460,735 (GRCm39)	missense	possibly damaging	0.46
R4909:Hectd4	UTSW	5	121,401,954 (GRCm39)	missense	probably benign	0.18
R4922:Hectd4	UTSW	5	121,497,378 (GRCm39)	missense	possibly damaging	0.86
R4925:Hectd4	UTSW	5	121,460,753 (GRCm39)	missense	possibly damaging	0.83
R5004:Hectd4	UTSW	5	121,467,628 (GRCm39)	missense	possibly damaging	0.93
R5004:Hectd4	UTSW	5	121,466,262 (GRCm39)	splice site	probably null
R5129:Hectd4	UTSW	5	121,481,573 (GRCm39)	missense	possibly damaging	0.87
R5217:Hectd4	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
R5267:Hectd4	UTSW	5	121,482,887 (GRCm39)	missense	probably benign	0.28
R5344:Hectd4	UTSW	5	121,481,739 (GRCm39)	missense	probably benign	0.28
R5345:Hectd4	UTSW	5	121,402,037 (GRCm39)	missense	possibly damaging	0.85
R5347:Hectd4	UTSW	5	121,442,511 (GRCm39)	missense	probably benign	0.33
R5360:Hectd4	UTSW	5	121,453,464 (GRCm39)	missense	possibly damaging	0.90
R5363:Hectd4	UTSW	5	121,448,666 (GRCm39)	missense	probably benign	0.04
R5445:Hectd4	UTSW	5	121,404,337 (GRCm39)	missense	probably benign	0.00
R5479:Hectd4	UTSW	5	121,445,011 (GRCm39)	missense	probably benign
R5507:Hectd4	UTSW	5	121,419,164 (GRCm39)	missense	unknown
R5552:Hectd4	UTSW	5	121,480,914 (GRCm39)	missense	possibly damaging	0.96
R5691:Hectd4	UTSW	5	121,486,878 (GRCm39)	missense	possibly damaging	0.85
R5745:Hectd4	UTSW	5	121,491,565 (GRCm39)	missense	possibly damaging	0.96
R5757:Hectd4	UTSW	5	121,486,682 (GRCm39)	missense	possibly damaging	0.72
R5845:Hectd4	UTSW	5	121,445,587 (GRCm39)	critical splice donor site	probably null
R5869:Hectd4	UTSW	5	121,481,288 (GRCm39)	critical splice donor site	probably null
R5913:Hectd4	UTSW	5	121,462,037 (GRCm39)	missense	possibly damaging	0.83
R5920:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R5943:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.01
R6219:Hectd4	UTSW	5	121,446,941 (GRCm39)	missense	possibly damaging	0.92
R6250:Hectd4	UTSW	5	121,477,561 (GRCm39)	missense	possibly damaging	0.85
R6301:Hectd4	UTSW	5	121,392,283 (GRCm39)	missense	possibly damaging	0.91
R6428:Hectd4	UTSW	5	121,488,508 (GRCm39)	missense	possibly damaging	0.53
R6446:Hectd4	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
R6453:Hectd4	UTSW	5	121,488,655 (GRCm39)	missense	probably damaging	1.00
R6513:Hectd4	UTSW	5	121,494,259 (GRCm39)	splice site	probably null
R6540:Hectd4	UTSW	5	121,441,634 (GRCm39)	missense	probably benign	0.33
R6706:Hectd4	UTSW	5	121,458,147 (GRCm39)	missense	possibly damaging	0.92
R6720:Hectd4	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
R6736:Hectd4	UTSW	5	121,415,788 (GRCm39)	missense	possibly damaging	0.86
R6776:Hectd4	UTSW	5	121,491,574 (GRCm39)	missense	possibly damaging	0.85
R7033:Hectd4	UTSW	5	121,502,631 (GRCm39)	missense	possibly damaging	0.86
R7038:Hectd4	UTSW	5	121,437,660 (GRCm39)	missense	possibly damaging	0.90
R7175:Hectd4	UTSW	5	121,411,692 (GRCm39)	missense	possibly damaging	0.85
R7180:Hectd4	UTSW	5	121,446,405 (GRCm39)	missense	probably benign	0.01
R7234:Hectd4	UTSW	5	121,467,136 (GRCm39)	missense	possibly damaging	0.53
R7253:Hectd4	UTSW	5	121,452,944 (GRCm39)	missense	possibly damaging	0.66
R7349:Hectd4	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
R7450:Hectd4	UTSW	5	121,419,995 (GRCm39)	missense	probably benign	0.00
R7467:Hectd4	UTSW	5	121,462,024 (GRCm39)	missense	possibly damaging	0.66
R7475:Hectd4	UTSW	5	121,496,196 (GRCm39)	splice site	probably null
R7482:Hectd4	UTSW	5	121,501,941 (GRCm39)	missense	possibly damaging	0.71
R7512:Hectd4	UTSW	5	121,435,172 (GRCm39)	missense	possibly damaging	0.72
R7525:Hectd4	UTSW	5	121,481,728 (GRCm39)	missense	possibly damaging	0.70
R7559:Hectd4	UTSW	5	121,453,573 (GRCm39)	splice site	probably null
R7560:Hectd4	UTSW	5	121,392,405 (GRCm39)	missense	possibly damaging	0.53
R7561:Hectd4	UTSW	5	121,429,288 (GRCm39)	missense	possibly damaging	0.91
R7576:Hectd4	UTSW	5	121,487,522 (GRCm39)	missense	possibly damaging	0.91
R7584:Hectd4	UTSW	5	121,456,798 (GRCm39)	missense	possibly damaging	0.83
R7648:Hectd4	UTSW	5	121,392,434 (GRCm39)	missense	possibly damaging	0.73
R7663:Hectd4	UTSW	5	121,462,094 (GRCm39)	missense	probably benign	0.06
R7692:Hectd4	UTSW	5	121,459,627 (GRCm39)	missense	possibly damaging	0.46
R7725:Hectd4	UTSW	5	121,358,680 (GRCm39)	missense	unknown
R7731:Hectd4	UTSW	5	121,445,077 (GRCm39)	missense	probably benign	0.00
R7732:Hectd4	UTSW	5	121,474,692 (GRCm39)	missense	probably benign	0.14
R7782:Hectd4	UTSW	5	121,443,784 (GRCm39)	missense	possibly damaging	0.53
R7854:Hectd4	UTSW	5	121,467,631 (GRCm39)	missense	probably benign	0.27
R7898:Hectd4	UTSW	5	121,469,880 (GRCm39)	missense	probably benign	0.18
R7910:Hectd4	UTSW	5	121,392,291 (GRCm39)	missense	possibly damaging	0.86
R7962:Hectd4	UTSW	5	121,448,692 (GRCm39)	missense	probably damaging	0.98
R8003:Hectd4	UTSW	5	121,477,581 (GRCm39)	missense	possibly damaging	0.85
R8098:Hectd4	UTSW	5	121,459,461 (GRCm39)	missense	possibly damaging	0.46
R8110:Hectd4	UTSW	5	121,471,012 (GRCm39)	missense	possibly damaging	0.96
R8118:Hectd4	UTSW	5	121,424,439 (GRCm39)	missense	probably benign	0.33
R8171:Hectd4	UTSW	5	121,456,819 (GRCm39)	missense	possibly damaging	0.82
R8234:Hectd4	UTSW	5	121,477,607 (GRCm39)	missense	possibly damaging	0.72
R8289:Hectd4	UTSW	5	121,404,424 (GRCm39)	missense	possibly damaging	0.53
R8292:Hectd4	UTSW	5	121,455,288 (GRCm39)	missense	possibly damaging	0.66
R8348:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8397:Hectd4	UTSW	5	121,397,957 (GRCm39)	missense	probably damaging	0.98
R8436:Hectd4	UTSW	5	121,446,421 (GRCm39)	missense	possibly damaging	0.90
R8436:Hectd4	UTSW	5	121,481,210 (GRCm39)	missense	probably benign	0.00
R8443:Hectd4	UTSW	5	121,467,172 (GRCm39)	missense	possibly damaging	0.72
R8448:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8516:Hectd4	UTSW	5	121,487,073 (GRCm39)	missense	possibly damaging	0.53
R8519:Hectd4	UTSW	5	121,442,489 (GRCm39)	nonsense	probably null
R8553:Hectd4	UTSW	5	121,491,661 (GRCm39)	missense	possibly damaging	0.73
R8557:Hectd4	UTSW	5	121,448,714 (GRCm39)	missense	possibly damaging	0.66
R8725:Hectd4	UTSW	5	121,488,557 (GRCm39)	missense	probably damaging	1.00
R8751:Hectd4	UTSW	5	121,501,838 (GRCm39)	nonsense	probably null
R8769:Hectd4	UTSW	5	121,419,936 (GRCm39)	missense	possibly damaging	0.53
R8803:Hectd4	UTSW	5	121,461,994 (GRCm39)	missense	probably benign	0.01
R8887:Hectd4	UTSW	5	121,433,541 (GRCm39)	missense	probably benign	0.44
R8982:Hectd4	UTSW	5	121,466,305 (GRCm39)	missense	probably benign	0.02
R8988:Hectd4	UTSW	5	121,415,819 (GRCm39)	missense	possibly damaging	0.86
R8991:Hectd4	UTSW	5	121,496,347 (GRCm39)	missense	probably benign	0.33
R8994:Hectd4	UTSW	5	121,441,629 (GRCm39)	missense	probably benign	0.33
R8995:Hectd4	UTSW	5	121,392,422 (GRCm39)	missense	possibly damaging	0.96
R9049:Hectd4	UTSW	5	121,451,955 (GRCm39)	missense	possibly damaging	0.92
R9093:Hectd4	UTSW	5	121,411,677 (GRCm39)	missense	probably benign	0.14
R9106:Hectd4	UTSW	5	121,467,619 (GRCm39)	missense	possibly damaging	0.53
R9137:Hectd4	UTSW	5	121,496,238 (GRCm39)	missense	possibly damaging	0.53
R9146:Hectd4	UTSW	5	121,487,097 (GRCm39)	missense	probably benign	0.33
R9154:Hectd4	UTSW	5	121,391,967 (GRCm39)	missense
R9162:Hectd4	UTSW	5	121,445,042 (GRCm39)	missense	possibly damaging	0.66
R9166:Hectd4	UTSW	5	121,446,690 (GRCm39)	missense	probably damaging	0.96
R9183:Hectd4	UTSW	5	121,437,551 (GRCm39)	missense	possibly damaging	0.51
R9207:Hectd4	UTSW	5	121,433,496 (GRCm39)	missense	possibly damaging	0.86
R9291:Hectd4	UTSW	5	121,487,028 (GRCm39)	missense	probably benign	0.14
R9300:Hectd4	UTSW	5	121,486,952 (GRCm39)	missense	probably benign	0.33
R9314:Hectd4	UTSW	5	121,437,708 (GRCm39)	critical splice donor site	probably null
R9381:Hectd4	UTSW	5	121,472,492 (GRCm39)	missense	possibly damaging	0.53
R9432:Hectd4	UTSW	5	121,460,864 (GRCm39)	missense	probably benign	0.01
R9491:Hectd4	UTSW	5	121,452,981 (GRCm39)	missense	probably damaging	0.97
R9532:Hectd4	UTSW	5	121,502,616 (GRCm39)	missense	probably benign	0.00
R9557:Hectd4	UTSW	5	121,459,617 (GRCm39)	missense	possibly damaging	0.66
R9561:Hectd4	UTSW	5	121,472,532 (GRCm39)	missense	possibly damaging	0.53
R9593:Hectd4	UTSW	5	121,424,844 (GRCm39)	nonsense	probably null
R9704:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9705:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9712:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9713:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9726:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9732:Hectd4	UTSW	5	121,392,254 (GRCm39)	nonsense	probably null
R9750:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9752:Hectd4	UTSW	5	121,472,415 (GRCm39)	missense	possibly damaging	0.85
R9752:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9772:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
X0026:Hectd4	UTSW	5	121,487,700 (GRCm39)	missense	probably benign	0.04
X0027:Hectd4	UTSW	5	121,459,467 (GRCm39)	missense	probably benign	0.27
Z1088:Hectd4	UTSW	5	121,433,566 (GRCm39)	splice site	probably null
Z1177:Hectd4	UTSW	5	121,496,383 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TTGATCATTCTTCAATTGTGCCGG -3'
(R):5'- GTGGTGTCAGGTTCAGAAGC -3'

Sequencing Primer
(F):5'- CTTCAATTGTGCCGGGCTGC -3'
(R):5'- GAGAGAACCGTCTGACATCCTTAAAG -3'

Posted On

2014-10-02