Mutagenetix > Incidental Mutation

Incidental Mutation 'R2202:Or10a2'

238746

Institutional Source

Beutler Lab

Gene Symbol

Or10a2

Ensembl Gene

ENSMUSG00000049674

Gene Name

olfactory receptor family 10 subfamily A member 2

Synonyms

GA_x6K02T2PBJ9-9453401-9454354, Olfr714, P4, MOR263-2

MMRRC Submission

040204-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R2202 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

106673037-106673990 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 106673523 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 163 (W163R)

Ref Sequence

ENSEMBL: ENSMUSP00000151106 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054629] [ENSMUST00000214429]

AlphaFold

Q7TRN0

Predicted Effect

probably damaging
Transcript: ENSMUST00000054629
AA Change: W163R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000095742
Gene: ENSMUSG00000049674
AA Change: W163R

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	309	8.5e-56	PFAM
Pfam:7tm_1	42	291	3.5e-24	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000207951

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000208139

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214123

Predicted Effect

probably damaging
Transcript: ENSMUST00000214429
AA Change: W163R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.5%
20x: 95.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a reporter allele exhibit abnormal olfactory sensory neuron projections. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	T	C	5: 64,056,011 (GRCm39)	V249A	possibly damaging	Het
9930111J21Rik2	C	G	11: 48,910,149 (GRCm39)	L761F	probably damaging	Het
Abca1	G	A	4: 53,090,291 (GRCm39)	T386I	probably damaging	Het
Abca14	T	C	7: 119,888,764 (GRCm39)	Y1237H	probably benign	Het
Abi3bp	C	T	16: 56,471,088 (GRCm39)	R578*	probably null	Het
Abi3bp	T	G	16: 56,433,566 (GRCm39)	L550R	probably benign	Het
Adamts7	A	T	9: 90,062,729 (GRCm39)	K394N	probably damaging	Het
Ahdc1	A	G	4: 132,793,220 (GRCm39)	E1487G	possibly damaging	Het
AI987944	T	C	7: 41,023,950 (GRCm39)	E343G	probably damaging	Het
Ankrd26	T	A	6: 118,500,843 (GRCm39)	H876L	possibly damaging	Het
Atg16l1	A	C	1: 87,694,737 (GRCm39)	Q138P	probably benign	Het
Atp1b1	G	T	1: 164,281,084 (GRCm39)	T11K	probably benign	Het
Calr3	A	G	8: 73,188,683 (GRCm39)	L40S	probably damaging	Het
Ccar1	A	C	10: 62,581,066 (GRCm39)	D1119E	unknown	Het
Cdan1	C	A	2: 120,551,241 (GRCm39)	C1093F	probably damaging	Het
Cdk12	T	G	11: 98,101,464 (GRCm39)	S441A	unknown	Het
Ces4a	T	A	8: 105,872,746 (GRCm39)	V333E	probably damaging	Het
Cfap61	T	C	2: 146,056,600 (GRCm39)	L1193P	probably damaging	Het
Chd2	A	T	7: 73,128,416 (GRCm39)	D856E	probably benign	Het
Chil3	T	C	3: 106,071,562 (GRCm39)	D34G	probably benign	Het
Cln3	T	A	7: 126,178,390 (GRCm39)	H211L	probably benign	Het
Cpa1	A	G	6: 30,641,818 (GRCm39)	D214G	probably damaging	Het
Cttnbp2	T	G	6: 18,408,693 (GRCm39)	D976A	probably benign	Het
Dcstamp	T	A	15: 39,617,708 (GRCm39)	V39E	probably damaging	Het
Dicer1	C	T	12: 104,697,297 (GRCm39)	V87M	possibly damaging	Het
Duox1	A	G	2: 122,175,194 (GRCm39)	T1331A	probably benign	Het
Elapor1	C	T	3: 108,382,359 (GRCm39)	G270E	probably damaging	Het
Fam184a	T	A	10: 53,528,530 (GRCm39)	Q29L	probably damaging	Het
Fcer2a	T	C	8: 3,738,557 (GRCm39)	E60G	possibly damaging	Het
Flnc	C	T	6: 29,459,507 (GRCm39)	P2536S	probably damaging	Het
Fnbp1l	A	T	3: 122,340,611 (GRCm39)	M463K	probably benign	Het
Garem2	A	G	5: 30,319,762 (GRCm39)	D408G	probably benign	Het
Gm6370	T	A	5: 146,430,539 (GRCm39)	D241E	probably benign	Het
Gpc3	T	A	X: 51,486,083 (GRCm39)	I344F	probably damaging	Het
Gtf2e1	T	A	16: 37,331,904 (GRCm39)	E390D	possibly damaging	Het
Hmgcs2	T	A	3: 98,198,499 (GRCm39)	I134N	probably damaging	Het
Il15ra	T	A	2: 11,723,155 (GRCm39)		probably null	Het
Ints8	A	T	4: 11,225,712 (GRCm39)	M615K	possibly damaging	Het
Irak1	G	A	X: 73,060,744 (GRCm39)	T193I	probably damaging	Het
Jmjd1c	A	G	10: 67,075,242 (GRCm39)		probably null	Het
Knstrn	T	A	2: 118,661,456 (GRCm39)		probably null	Het
Letm1	C	A	5: 33,926,830 (GRCm39)	V156L	possibly damaging	Het
Lrrc24	G	A	15: 76,607,111 (GRCm39)	P95L	probably damaging	Het
Map2k2	T	C	10: 80,955,213 (GRCm39)	S14P	probably damaging	Het
Me3	T	C	7: 89,499,589 (GRCm39)	Y535H	probably damaging	Het
Nfatc2ip	T	C	7: 125,990,467 (GRCm39)	E178G	probably benign	Het
Nop56	T	A	2: 130,119,488 (GRCm39)	I51N	probably damaging	Het
Ntn4	C	T	10: 93,543,215 (GRCm39)	R314W	probably damaging	Het
Nudt5	A	T	2: 5,860,794 (GRCm39)	I22F	possibly damaging	Het
Or2m12	C	T	16: 19,105,047 (GRCm39)	A149T	probably benign	Het
Or4p23	C	G	2: 88,576,953 (GRCm39)	G93A	probably benign	Het
Pbrm1	A	G	14: 30,754,406 (GRCm39)	D142G	possibly damaging	Het
Pdcl	A	T	2: 37,242,056 (GRCm39)	N231K	probably benign	Het
Pdlim2	C	T	14: 70,402,228 (GRCm39)	R296H	probably damaging	Het
Pid1	T	A	1: 84,016,159 (GRCm39)	I69F	probably damaging	Het
Pkhd1	A	G	1: 20,607,584 (GRCm39)	S1091P	probably benign	Het
Plcb4	T	C	2: 135,844,514 (GRCm39)	I144T	probably benign	Het
Plxnd1	T	C	6: 115,939,725 (GRCm39)	N1418S	probably benign	Het
Pmm1	T	C	15: 81,840,601 (GRCm39)	T82A	probably benign	Het
Prrc2b	C	A	2: 32,113,476 (GRCm39)	Q1970K	probably damaging	Het
Ptprz1	C	T	6: 23,000,649 (GRCm39)	T913M	possibly damaging	Het
Ralgapa1	T	C	12: 55,659,585 (GRCm39)		probably null	Het
Rbm8a2	T	C	1: 175,806,420 (GRCm39)	E19G	possibly damaging	Het
Rgs3	T	C	4: 62,608,741 (GRCm39)	S336P	probably damaging	Het
Saxo5	A	T	8: 3,529,028 (GRCm39)	D201V	probably benign	Het
Serpina11	G	T	12: 103,952,233 (GRCm39)	T179K	probably damaging	Het
Serpina1f	T	C	12: 103,659,655 (GRCm39)	N209S	possibly damaging	Het
Serpinf2	G	A	11: 75,327,588 (GRCm39)	T159I	probably benign	Het
Slc19a1	T	C	10: 76,877,758 (GRCm39)	C98R	possibly damaging	Het
Slc22a20	A	T	19: 6,021,553 (GRCm39)	I483N	possibly damaging	Het
Spata31d1d	A	G	13: 59,879,435 (GRCm39)	C34R	possibly damaging	Het
Stoml2	G	T	4: 43,030,243 (GRCm39)	Y119*	probably null	Het
Susd1	A	G	4: 59,349,843 (GRCm39)	L531P	possibly damaging	Het
Timd5	A	G	11: 46,419,394 (GRCm39)	N70S	probably benign	Het
Tln1	C	T	4: 43,553,083 (GRCm39)		probably null	Het
Tmem39b	A	C	4: 129,587,716 (GRCm39)	S32A	probably benign	Het
Tmx3	T	C	18: 90,546,037 (GRCm39)	F206S	probably damaging	Het
Tnfsf14	T	C	17: 57,497,638 (GRCm39)	D198G	possibly damaging	Het
Trappc10	A	G	10: 78,034,876 (GRCm39)		probably null	Het
Tssk2	A	G	16: 17,716,603 (GRCm39)	D2G	possibly damaging	Het
Ttn	T	C	2: 76,601,985 (GRCm39)	N18559S	possibly damaging	Het
Ube3b	G	A	5: 114,527,135 (GRCm39)	V118M	probably damaging	Het
Vmn1r23	T	C	6: 57,903,604 (GRCm39)	D58G	probably benign	Het
Vmn2r53	T	A	7: 12,335,366 (GRCm39)	Y98F	probably damaging	Het
Vmn2r58	T	A	7: 41,513,594 (GRCm39)	N350Y	probably benign	Het
Vmn2r82	A	T	10: 79,192,519 (GRCm39)	H32L	probably benign	Het
Wsb1	T	C	11: 79,131,212 (GRCm39)	I395V	probably benign	Het
Zbtb47	A	G	9: 121,591,703 (GRCm39)	T8A	possibly damaging	Het
Zfat	T	C	15: 68,051,709 (GRCm39)	D695G	probably benign	Het
Zfp358	G	T	8: 3,546,995 (GRCm39)	V526F	possibly damaging	Het
Zmat1	A	G	X: 133,873,861 (GRCm39)	L476P	possibly damaging	Het

Other mutations in Or10a2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Or10a2	APN	7	106,673,700 (GRCm39)	missense	probably damaging	0.98
IGL02562:Or10a2	APN	7	106,673,769 (GRCm39)	missense	probably benign	0.03
IGL02597:Or10a2	APN	7	106,673,646 (GRCm39)	missense	possibly damaging	0.89
IGL02598:Or10a2	APN	7	106,673,923 (GRCm39)	missense	possibly damaging	0.64
IGL02981:Or10a2	APN	7	106,673,758 (GRCm39)	missense	probably damaging	1.00
BB004:Or10a2	UTSW	7	106,673,496 (GRCm39)	missense	probably benign	0.00
BB014:Or10a2	UTSW	7	106,673,496 (GRCm39)	missense	probably benign	0.00
R0064:Or10a2	UTSW	7	106,673,487 (GRCm39)	missense	probably benign	0.02
R0064:Or10a2	UTSW	7	106,673,487 (GRCm39)	missense	probably benign	0.02
R0518:Or10a2	UTSW	7	106,673,965 (GRCm39)	missense	possibly damaging	0.81
R0521:Or10a2	UTSW	7	106,673,965 (GRCm39)	missense	possibly damaging	0.81
R1661:Or10a2	UTSW	7	106,673,481 (GRCm39)	missense	probably damaging	1.00
R1665:Or10a2	UTSW	7	106,673,481 (GRCm39)	missense	probably damaging	1.00
R2069:Or10a2	UTSW	7	106,673,826 (GRCm39)	nonsense	probably null
R3884:Or10a2	UTSW	7	106,673,110 (GRCm39)	missense	possibly damaging	0.72
R4362:Or10a2	UTSW	7	106,673,799 (GRCm39)	missense	probably damaging	0.99
R4618:Or10a2	UTSW	7	106,673,761 (GRCm39)	missense	probably damaging	1.00
R5375:Or10a2	UTSW	7	106,673,080 (GRCm39)	missense	probably benign	0.05
R5654:Or10a2	UTSW	7	106,673,394 (GRCm39)	missense	probably damaging	1.00
R6228:Or10a2	UTSW	7	106,673,343 (GRCm39)	missense	probably damaging	1.00
R7196:Or10a2	UTSW	7	106,673,935 (GRCm39)	missense	probably benign	0.01
R7202:Or10a2	UTSW	7	106,673,448 (GRCm39)	missense	probably benign	0.01
R7232:Or10a2	UTSW	7	106,673,062 (GRCm39)	missense	probably benign	0.03
R7927:Or10a2	UTSW	7	106,673,496 (GRCm39)	missense	probably benign	0.00
R9157:Or10a2	UTSW	7	106,673,214 (GRCm39)	missense	probably damaging	1.00
R9526:Or10a2	UTSW	7	106,673,739 (GRCm39)	nonsense	probably null
R9629:Or10a2	UTSW	7	106,673,164 (GRCm39)	missense	probably damaging	1.00
Z1088:Or10a2	UTSW	7	106,673,612 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- TCTGATTGCCCAGGACACAAG -3'
(R):5'- TGAACATAGGATCAGCAGGC -3'

Sequencing Primer
(F):5'- GACACAAGCATCTCCTTCCTGG -3'
(R):5'- CAGGCAGGGTATCATGACAACC -3'

Posted On

2014-10-02