Mutagenetix > Incidental Mutation

Incidental Mutation 'R2228:Zdhhc4'

239846

Institutional Source

Beutler Lab

Gene Symbol

Zdhhc4

Ensembl Gene

ENSMUSG00000001844

Gene Name

zinc finger, DHHC domain containing 4

Synonyms

1810021D01Rik, 2900029I10Rik

MMRRC Submission

040229-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R2228 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

143302244-143315007 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 143306162 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Glycine at position 189 (W189G)

Ref Sequence

ENSEMBL: ENSMUSP00000124813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001900] [ENSMUST00000159813] [ENSMUST00000159941] [ENSMUST00000161915] [ENSMUST00000162066] [ENSMUST00000162358] [ENSMUST00000162941]

AlphaFold

Q9D6H5

Predicted Effect

probably damaging
Transcript: ENSMUST00000001900
AA Change: W189G

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000001900
Gene: ENSMUSG00000001844
AA Change: W189G

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	294	5e-38	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159718

Predicted Effect

probably benign
Transcript: ENSMUST00000159813

SMART Domains

Protein: ENSMUSP00000137935
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	175	3.6e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000159941

SMART Domains

Protein: ENSMUSP00000124026
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	178	2.2e-16	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161333

Predicted Effect

probably damaging
Transcript: ENSMUST00000161915
AA Change: W189G

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000124813
Gene: ENSMUSG00000001844
AA Change: W189G

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
transmembrane domain	100	122	N/A	INTRINSIC
Pfam:zf-DHHC	144	294	9.7e-37	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000162066

SMART Domains

Protein: ENSMUSP00000125130
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162287

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200220

Predicted Effect

probably benign
Transcript: ENSMUST00000162358

SMART Domains

Protein: ENSMUSP00000124416
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
transmembrane domain	100	122	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162941

SMART Domains

Protein: ENSMUSP00000124997
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	176	4.2e-14	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932414N04Rik	A	G	2: 68,559,935 (GRCm39)	T242A	probably benign	Het
Adam24	A	T	8: 41,133,404 (GRCm39)	I291L	probably benign	Het
Adamts16	G	A	13: 70,927,637 (GRCm39)		probably benign	Het
Adcy8	C	A	15: 64,694,056 (GRCm39)	R407L	possibly damaging	Het
Aldh3b2	C	T	19: 4,031,133 (GRCm39)	P461S	probably benign	Het
Araf	T	C	X: 20,717,912 (GRCm39)	F144L	probably benign	Het
Atp11b	G	T	3: 35,861,091 (GRCm39)	D193Y	probably damaging	Het
Atp6v1b2	C	A	8: 69,555,411 (GRCm39)		probably null	Het
Bclaf1	T	A	10: 20,215,624 (GRCm39)		probably benign	Het
Cadps	A	G	14: 12,465,935 (GRCm38)	Y987H	probably benign	Het
Capn6	T	G	X: 142,587,785 (GRCm39)	T498P	possibly damaging	Het
Ccdc153	T	A	9: 44,154,314 (GRCm39)	L47Q	probably damaging	Het
Ccdc180	G	T	4: 45,948,856 (GRCm39)		probably null	Het
Cep162	G	T	9: 87,126,384 (GRCm39)	T176K	probably benign	Het
Cpsf2	A	G	12: 101,956,088 (GRCm39)	D297G	probably benign	Het
Entpd8	T	C	2: 24,975,028 (GRCm39)	M453T	probably damaging	Het
Eprs1	T	A	1: 185,099,734 (GRCm39)	L18Q	probably damaging	Het
Fem1b	G	T	9: 62,704,020 (GRCm39)	C413*	probably null	Het
Flrt1	T	A	19: 7,072,723 (GRCm39)	D608V	probably damaging	Het
Fstl5	C	A	3: 76,389,659 (GRCm39)	N285K	probably damaging	Het
Golga1	T	C	2: 38,913,183 (GRCm39)	D543G	probably benign	Het
Hivep2	A	G	10: 14,004,107 (GRCm39)	H235R	probably damaging	Het
Htr2c	G	C	X: 145,977,186 (GRCm39)	W325C	probably damaging	Het
Htr2c	G	T	X: 145,977,188 (GRCm39)	C326F	probably damaging	Het
Ifit1bl2	G	T	19: 34,596,630 (GRCm39)	L329M	possibly damaging	Het
Igsf9b	T	C	9: 27,244,792 (GRCm39)	S920P	probably damaging	Het
Macroh2a1	C	T	13: 56,232,075 (GRCm39)	G235S	probably damaging	Het
Mcm8	T	A	2: 132,662,041 (GRCm39)	I125K	possibly damaging	Het
Micall1	C	T	15: 79,014,036 (GRCm39)	R644W	probably damaging	Het
Myo16	G	A	8: 10,644,905 (GRCm39)	D1746N	possibly damaging	Het
Myo9a	A	G	9: 59,801,463 (GRCm39)	E1887G	probably benign	Het
Nbn	A	G	4: 15,970,904 (GRCm39)	T296A	probably benign	Het
Nckap1l	T	C	15: 103,364,361 (GRCm39)		probably null	Het
Neb	T	C	2: 52,123,007 (GRCm39)	R3734G	probably benign	Het
Nup93	C	T	8: 95,030,819 (GRCm39)	T305I	probably benign	Het
Or5bw2	A	T	7: 6,573,802 (GRCm39)	M271L	probably benign	Het
P2ry4	A	G	X: 99,637,553 (GRCm39)	L115P	probably damaging	Het
Pmepa1	G	A	2: 173,069,926 (GRCm39)	R210W	probably damaging	Het
Ppp1r26	T	C	2: 28,343,798 (GRCm39)	F1143L	possibly damaging	Het
Ppp1r3c	C	A	19: 36,711,098 (GRCm39)	R224L	probably benign	Het
Ptafr	T	G	4: 132,306,691 (GRCm39)	I27R	possibly damaging	Het
Pwwp2b	T	C	7: 138,835,104 (GRCm39)	C182R	probably damaging	Het
Reln	A	G	5: 22,192,076 (GRCm39)	F1455L	possibly damaging	Het
Rufy1	C	T	11: 50,288,611 (GRCm39)		probably null	Het
Samd9l	T	C	6: 3,376,910 (GRCm39)	H117R	probably benign	Het
Sfxn4	C	T	19: 60,839,458 (GRCm39)	G200E	probably damaging	Het
Sipa1l3	T	A	7: 29,077,364 (GRCm39)	K803*	probably null	Het
Smim23	C	A	11: 32,771,870 (GRCm39)	Q65H	probably damaging	Het
Spata18	A	T	5: 73,824,244 (GRCm39)	I156L	possibly damaging	Het
Sppl2b	G	A	10: 80,701,451 (GRCm39)	V389M	probably damaging	Het
Srbd1	T	C	17: 86,292,651 (GRCm39)	I973V	probably damaging	Het
Ssx2ip	C	T	3: 146,123,531 (GRCm39)	P10L	probably damaging	Het
Taf2	A	C	15: 54,928,042 (GRCm39)	D120E	possibly damaging	Het
Tanc1	T	C	2: 59,555,068 (GRCm39)	L42S	probably benign	Het
Tex15	C	A	8: 34,061,265 (GRCm39)	H232N	probably benign	Het
Tg	A	T	15: 66,545,860 (GRCm39)	Q194L	probably damaging	Het
Tnfrsf22	C	T	7: 143,198,513 (GRCm39)		probably null	Het
Tro	A	T	X: 149,438,477 (GRCm39)	M60K	probably benign	Het
Ttc38	G	A	15: 85,728,704 (GRCm39)	V219I	probably benign	Het
U2af2	T	C	7: 5,078,672 (GRCm39)	I417T	probably damaging	Het
Ube2r2	C	T	4: 41,174,044 (GRCm39)	H61Y	probably benign	Het
Ugcg	C	T	4: 59,207,798 (GRCm39)	P46S	probably benign	Het
Vmn1r113	C	T	7: 20,521,832 (GRCm39)	S208F	probably damaging	Het
Wdr35	A	G	12: 9,024,955 (GRCm39)	K16E	possibly damaging	Het
Wwp1	A	T	4: 19,641,745 (GRCm39)	Y437N	probably damaging	Het
Zfp512	A	T	5: 31,622,919 (GRCm39)	K73N	probably damaging	Het

Other mutations in Zdhhc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02686:Zdhhc4	APN	5	143,306,146 (GRCm39)	missense	probably damaging	1.00
R2022:Zdhhc4	UTSW	5	143,307,538 (GRCm39)	missense	probably damaging	1.00
R2138:Zdhhc4	UTSW	5	143,310,017 (GRCm39)	nonsense	probably null
R4298:Zdhhc4	UTSW	5	143,309,997 (GRCm39)	missense	probably damaging	0.96
R4305:Zdhhc4	UTSW	5	143,310,099 (GRCm39)	intron	probably benign
R4722:Zdhhc4	UTSW	5	143,307,536 (GRCm39)	missense	probably damaging	1.00
R4773:Zdhhc4	UTSW	5	143,311,931 (GRCm39)	missense	possibly damaging	0.50
R5000:Zdhhc4	UTSW	5	143,310,688 (GRCm39)	missense	probably damaging	0.98
R5063:Zdhhc4	UTSW	5	143,302,377 (GRCm39)	missense	probably damaging	1.00
R5341:Zdhhc4	UTSW	5	143,311,915 (GRCm39)	missense	probably benign	0.01
R5945:Zdhhc4	UTSW	5	143,310,641 (GRCm39)	missense	probably damaging	1.00
R5956:Zdhhc4	UTSW	5	143,310,604 (GRCm39)	intron	probably benign
R7284:Zdhhc4	UTSW	5	143,307,646 (GRCm39)	missense	probably benign	0.01
R7843:Zdhhc4	UTSW	5	143,306,031 (GRCm39)	missense	probably damaging	1.00
R7955:Zdhhc4	UTSW	5	143,307,619 (GRCm39)	missense	probably damaging	1.00
R8261:Zdhhc4	UTSW	5	143,307,588 (GRCm39)	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- AGTAGCTTCATGTTTTCCTGAGAC -3'
(R):5'- TCTGAAAGCCAGGTGACAGC -3'

Sequencing Primer
(F):5'- ATGTTTTCCTGAGACCCACCG -3'
(R):5'- TGACAGCAGCTGCCCTG -3'

Posted On

2014-10-15