Mutagenetix > Incidental Mutation

Incidental Mutation 'R2249:Map1a'

240762

Institutional Source

Beutler Lab

Gene Symbol

Map1a

Ensembl Gene

ENSMUSG00000027254

Gene Name

microtubule-associated protein 1 A

Synonyms

Mtap1a, Mtap-1, 6330416M19Rik, Mtap1

MMRRC Submission

040249-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.235) question?

Stock #

R2249 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

121120081-121141313 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to C at 121130768 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Proline at position 528 (R528P)

Ref Sequence

ENSEMBL: ENSMUSP00000092223 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094639] [ENSMUST00000110639]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000094639
AA Change: R528P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000092223
Gene: ENSMUSG00000027254
AA Change: R528P

Domain	Start	End	E-Value	Type
Blast:Lactamase_B	286	538	2e-54	BLAST
SCOP:d1eq1a_	584	699	8e-5	SMART
low complexity region	743	755	N/A	INTRINSIC
low complexity region	820	833	N/A	INTRINSIC
low complexity region	852	867	N/A	INTRINSIC
low complexity region	897	911	N/A	INTRINSIC
low complexity region	1228	1239	N/A	INTRINSIC
low complexity region	1334	1344	N/A	INTRINSIC
low complexity region	1540	1555	N/A	INTRINSIC
coiled coil region	1573	1602	N/A	INTRINSIC
internal_repeat_1	1616	1726	7.66e-6	PROSPERO
coiled coil region	1747	1771	N/A	INTRINSIC
internal_repeat_1	1774	1888	7.66e-6	PROSPERO
low complexity region	2060	2084	N/A	INTRINSIC
low complexity region	2121	2133	N/A	INTRINSIC
low complexity region	2156	2169	N/A	INTRINSIC
low complexity region	2383	2396	N/A	INTRINSIC
low complexity region	2436	2460	N/A	INTRINSIC
low complexity region	2517	2541	N/A	INTRINSIC
low complexity region	2589	2600	N/A	INTRINSIC
low complexity region	2662	2682	N/A	INTRINSIC
low complexity region	2685	2704	N/A	INTRINSIC
low complexity region	2716	2728	N/A	INTRINSIC
low complexity region	2766	2790	N/A	INTRINSIC
low complexity region	2980	2988	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110639
AA Change: R290P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000106269
Gene: ENSMUSG00000027254
AA Change: R290P

Domain	Start	End	E-Value	Type
Blast:Lactamase_B	48	300	3e-54	BLAST
SCOP:d1eq1a_	346	461	1e-4	SMART
low complexity region	505	517	N/A	INTRINSIC
low complexity region	582	595	N/A	INTRINSIC
low complexity region	614	629	N/A	INTRINSIC
low complexity region	659	673	N/A	INTRINSIC
low complexity region	990	1001	N/A	INTRINSIC
low complexity region	1096	1106	N/A	INTRINSIC
low complexity region	1302	1317	N/A	INTRINSIC
coiled coil region	1335	1364	N/A	INTRINSIC
internal_repeat_1	1378	1488	5.43e-6	PROSPERO
coiled coil region	1509	1533	N/A	INTRINSIC
internal_repeat_1	1536	1650	5.43e-6	PROSPERO
low complexity region	1822	1846	N/A	INTRINSIC
low complexity region	1883	1895	N/A	INTRINSIC
low complexity region	1918	1931	N/A	INTRINSIC
low complexity region	2145	2158	N/A	INTRINSIC
low complexity region	2198	2222	N/A	INTRINSIC
low complexity region	2279	2303	N/A	INTRINSIC
low complexity region	2351	2362	N/A	INTRINSIC
low complexity region	2424	2444	N/A	INTRINSIC
low complexity region	2447	2466	N/A	INTRINSIC
low complexity region	2478	2490	N/A	INTRINSIC
low complexity region	2528	2552	N/A	INTRINSIC
low complexity region	2742	2750	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129000

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133283

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain. Expression of this gene is almost exclusively in the brain. Studies of the rat microtubule-associated protein 1A gene suggested a role in early events of spinal cord development. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit Purkinje cell degeneration. Mice homozygous for a spontaneous mutation exhibit mild ataxia and Purkinje cell degeneration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acvr1b	G	A	15: 101,100,975 (GRCm39)	R379Q	probably null	Het
Adam23	C	T	1: 63,574,335 (GRCm39)	Q276*	probably null	Het
Apob	A	T	12: 8,057,499 (GRCm39)	S1961C	probably damaging	Het
Atxn7l1	C	T	12: 33,408,839 (GRCm39)	P334S	probably damaging	Het
Ccnb1	T	C	13: 100,917,827 (GRCm39)	M258V	possibly damaging	Het
Cd34	A	C	1: 194,630,260 (GRCm39)	T65P	possibly damaging	Het
Cog6	A	G	3: 52,907,900 (GRCm39)		probably null	Het
Cp	T	C	3: 20,041,734 (GRCm39)	M953T	probably damaging	Het
Cwc27	C	T	13: 104,768,130 (GRCm39)	R455Q	unknown	Het
Dhx57	A	T	17: 80,588,663 (GRCm39)	D63E	probably damaging	Het
Elapor1	A	T	3: 108,378,726 (GRCm39)	Y409*	probably null	Het
Flt4	T	A	11: 49,536,786 (GRCm39)	M1252K	possibly damaging	Het
Gfral	C	T	9: 76,100,631 (GRCm39)	C269Y	probably damaging	Het
Itih4	C	T	14: 30,621,351 (GRCm39)	Q788*	probably null	Het
Kif18b	A	G	11: 102,803,214 (GRCm39)	S499P	probably benign	Het
Map3k5	T	C	10: 20,003,443 (GRCm39)	F1152L	probably damaging	Het
Marveld2	T	C	13: 100,748,599 (GRCm39)	D160G	probably benign	Het
Mtcl2	A	T	2: 156,882,013 (GRCm39)	C680S	probably benign	Het
Mug1	A	T	6: 121,847,469 (GRCm39)	M616L	probably benign	Het
Ndst4	A	T	3: 125,231,823 (GRCm39)	I131F	probably benign	Het
Neto1	G	T	18: 86,479,399 (GRCm39)	A196S	probably benign	Het
Notum	A	G	11: 120,545,237 (GRCm39)	F441L	probably benign	Het
Nsmce4a	A	G	7: 130,140,769 (GRCm39)	I239T	probably benign	Het
Or51g1	A	T	7: 102,633,647 (GRCm39)	N241K	possibly damaging	Het
Or52z14	T	A	7: 103,252,943 (GRCm39)	D27E	probably benign	Het
Or5d35	A	T	2: 87,855,707 (GRCm39)	I214F	probably damaging	Het
Or6s1	T	A	14: 51,307,870 (GRCm39)	K327*	probably null	Het
Or6x1	A	T	9: 40,098,980 (GRCm39)	T190S	possibly damaging	Het
Or8k40	A	G	2: 86,584,398 (GRCm39)	M228T	probably damaging	Het
Pde4dip	A	T	3: 97,700,841 (GRCm39)	V221D	probably damaging	Het
Pkp1	T	C	1: 135,808,545 (GRCm39)	Y474C	probably damaging	Het
Ptk6	A	C	2: 180,838,173 (GRCm39)	H363Q	probably benign	Het
Rb1cc1	T	C	1: 6,342,948 (GRCm39)	W187R	probably damaging	Het
Rbfox3	A	T	11: 118,394,564 (GRCm39)	F132L	probably damaging	Het
Rcl1	A	T	19: 29,099,268 (GRCm39)	I188F	possibly damaging	Het
Scfd1	T	A	12: 51,462,299 (GRCm39)	S385T	possibly damaging	Het
Sema6d	A	G	2: 124,501,508 (GRCm39)	E483G	possibly damaging	Het
Semp2l2b	T	C	10: 21,943,015 (GRCm39)	I322V	possibly damaging	Het
Sipa1l1	T	C	12: 82,388,890 (GRCm39)	V372A	probably benign	Het
Slc17a6	G	A	7: 51,317,654 (GRCm39)	G429D	probably damaging	Het
Slc22a5	A	G	11: 53,774,532 (GRCm39)	V151A	possibly damaging	Het
Spidr	T	C	16: 15,936,787 (GRCm39)	D106G	probably damaging	Het
Tekt3	A	T	11: 62,974,778 (GRCm39)	T366S	probably benign	Het
Trpm3	T	C	19: 22,710,398 (GRCm39)	M281T	probably benign	Het
Ttn	A	G	2: 76,782,485 (GRCm39)	V917A	probably damaging	Het
Tubgcp4	A	T	2: 121,014,110 (GRCm39)	D221V	possibly damaging	Het
Ubr4	C	T	4: 139,176,232 (GRCm39)	R3153W	probably damaging	Het
Vmn1r89	A	C	7: 12,954,187 (GRCm39)	T308P	possibly damaging	Het
Vmn2r59	A	G	7: 41,708,326 (GRCm39)	I27T	probably benign	Het
Vps13c	T	G	9: 67,895,335 (GRCm39)		probably null	Het
Zfp142	A	G	1: 74,606,191 (GRCm39)	V1793A	probably damaging	Het
Zfp472	T	A	17: 33,197,109 (GRCm39)	C395S	possibly damaging	Het

Other mutations in Map1a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00419:Map1a	APN	2	121,129,508 (GRCm39)	missense	probably damaging	0.99
IGL00826:Map1a	APN	2	121,132,757 (GRCm39)	missense	possibly damaging	0.87
IGL01476:Map1a	APN	2	121,135,688 (GRCm39)	missense	probably damaging	1.00
IGL02029:Map1a	APN	2	121,133,779 (GRCm39)	missense	possibly damaging	0.57
IGL02100:Map1a	APN	2	121,133,327 (GRCm39)	missense	probably damaging	0.99
IGL02136:Map1a	APN	2	121,130,693 (GRCm39)	missense	probably damaging	1.00
IGL02146:Map1a	APN	2	121,129,927 (GRCm39)	missense	probably damaging	1.00
IGL02264:Map1a	APN	2	121,137,794 (GRCm39)	missense	probably damaging	1.00
IGL02456:Map1a	APN	2	121,129,134 (GRCm39)	missense	probably damaging	1.00
IGL02485:Map1a	APN	2	121,129,769 (GRCm39)	missense	probably damaging	1.00
IGL02535:Map1a	APN	2	121,132,658 (GRCm39)	nonsense	probably null
IGL02628:Map1a	APN	2	121,130,585 (GRCm39)	missense	probably damaging	1.00
IGL02721:Map1a	APN	2	121,134,518 (GRCm39)	missense	probably benign	0.44
IGL03273:Map1a	APN	2	121,130,719 (GRCm39)	missense	probably damaging	1.00
IGL03281:Map1a	APN	2	121,135,541 (GRCm39)	missense	probably damaging	1.00
IGL02991:Map1a	UTSW	2	121,132,091 (GRCm39)	missense	probably damaging	0.99
R0096:Map1a	UTSW	2	121,131,986 (GRCm39)	missense	probably damaging	1.00
R0096:Map1a	UTSW	2	121,131,986 (GRCm39)	missense	probably damaging	1.00
R0218:Map1a	UTSW	2	121,135,906 (GRCm39)	missense	probably benign	0.00
R0363:Map1a	UTSW	2	121,132,525 (GRCm39)	missense	probably damaging	1.00
R0450:Map1a	UTSW	2	121,136,255 (GRCm39)	missense	probably benign	0.27
R0469:Map1a	UTSW	2	121,136,255 (GRCm39)	missense	probably benign	0.27
R0477:Map1a	UTSW	2	121,132,582 (GRCm39)	missense	probably damaging	1.00
R0504:Map1a	UTSW	2	121,133,422 (GRCm39)	missense	probably benign	0.03
R0510:Map1a	UTSW	2	121,136,255 (GRCm39)	missense	probably benign	0.27
R0521:Map1a	UTSW	2	121,136,234 (GRCm39)	missense	probably damaging	1.00
R0601:Map1a	UTSW	2	121,129,083 (GRCm39)	missense	probably damaging	1.00
R0619:Map1a	UTSW	2	121,135,736 (GRCm39)	missense	probably damaging	0.96
R0633:Map1a	UTSW	2	121,138,495 (GRCm39)	missense	probably damaging	1.00
R0652:Map1a	UTSW	2	121,133,264 (GRCm39)	missense	probably benign	0.04
R0893:Map1a	UTSW	2	121,131,014 (GRCm39)	missense	probably damaging	1.00
R0960:Map1a	UTSW	2	121,132,124 (GRCm39)	missense	probably benign	0.16
R1115:Map1a	UTSW	2	121,137,859 (GRCm39)	splice site	probably null
R1166:Map1a	UTSW	2	121,130,741 (GRCm39)	missense	probably damaging	1.00
R1326:Map1a	UTSW	2	121,136,671 (GRCm39)	nonsense	probably null
R1331:Map1a	UTSW	2	121,136,701 (GRCm39)	nonsense	probably null
R1395:Map1a	UTSW	2	121,134,406 (GRCm39)	missense	probably benign	0.26
R1489:Map1a	UTSW	2	121,130,918 (GRCm39)	missense	possibly damaging	0.91
R1573:Map1a	UTSW	2	121,134,607 (GRCm39)	missense	probably benign	0.37
R1596:Map1a	UTSW	2	121,120,246 (GRCm39)	missense	probably benign	0.00
R1662:Map1a	UTSW	2	121,136,889 (GRCm39)	missense	possibly damaging	0.90
R1675:Map1a	UTSW	2	121,133,136 (GRCm39)	nonsense	probably null
R1919:Map1a	UTSW	2	121,137,493 (GRCm39)	missense	probably damaging	1.00
R2122:Map1a	UTSW	2	121,129,927 (GRCm39)	missense	probably damaging	1.00
R2126:Map1a	UTSW	2	121,129,122 (GRCm39)	missense	probably damaging	0.96
R2143:Map1a	UTSW	2	121,132,426 (GRCm39)	missense	probably damaging	1.00
R2172:Map1a	UTSW	2	121,138,413 (GRCm39)	missense	probably damaging	1.00
R2254:Map1a	UTSW	2	121,134,272 (GRCm39)	missense	possibly damaging	0.71
R2255:Map1a	UTSW	2	121,134,272 (GRCm39)	missense	possibly damaging	0.71
R3834:Map1a	UTSW	2	121,137,803 (GRCm39)	missense	probably damaging	1.00
R4011:Map1a	UTSW	2	121,130,608 (GRCm39)	missense	probably damaging	1.00
R4346:Map1a	UTSW	2	121,131,806 (GRCm39)	missense	probably benign	0.13
R4842:Map1a	UTSW	2	121,132,567 (GRCm39)	missense	probably damaging	1.00
R4933:Map1a	UTSW	2	121,136,386 (GRCm39)	missense	probably damaging	1.00
R4978:Map1a	UTSW	2	121,131,623 (GRCm39)	missense	probably benign	0.00
R4988:Map1a	UTSW	2	121,133,531 (GRCm39)	missense	probably benign	0.34
R5026:Map1a	UTSW	2	121,138,019 (GRCm39)	missense	possibly damaging	0.83
R5086:Map1a	UTSW	2	121,134,985 (GRCm39)	missense	probably damaging	1.00
R5155:Map1a	UTSW	2	121,132,867 (GRCm39)	missense	probably damaging	1.00
R5232:Map1a	UTSW	2	121,132,466 (GRCm39)	missense	probably damaging	1.00
R5311:Map1a	UTSW	2	121,132,868 (GRCm39)	missense	probably damaging	1.00
R5401:Map1a	UTSW	2	121,130,153 (GRCm39)	missense	probably damaging	1.00
R5465:Map1a	UTSW	2	121,136,506 (GRCm39)	missense	probably damaging	1.00
R5526:Map1a	UTSW	2	121,136,143 (GRCm39)	missense	probably damaging	1.00
R5642:Map1a	UTSW	2	121,136,524 (GRCm39)	missense	probably damaging	1.00
R5726:Map1a	UTSW	2	121,135,546 (GRCm39)	missense	probably damaging	1.00
R5817:Map1a	UTSW	2	121,129,391 (GRCm39)	missense	possibly damaging	0.81
R5855:Map1a	UTSW	2	121,134,155 (GRCm39)	missense	possibly damaging	0.74
R5917:Map1a	UTSW	2	121,135,697 (GRCm39)	missense	probably damaging	1.00
R5974:Map1a	UTSW	2	121,134,857 (GRCm39)	missense	probably benign	0.20
R5987:Map1a	UTSW	2	121,134,776 (GRCm39)	missense	possibly damaging	0.56
R6151:Map1a	UTSW	2	121,120,304 (GRCm39)	missense	probably benign	0.12
R6406:Map1a	UTSW	2	121,131,224 (GRCm39)	missense	probably damaging	1.00
R7014:Map1a	UTSW	2	121,130,720 (GRCm39)	missense	probably damaging	1.00
R7099:Map1a	UTSW	2	121,130,998 (GRCm39)	missense	probably benign	0.04
R7211:Map1a	UTSW	2	121,135,124 (GRCm39)	missense	probably benign	0.02
R7230:Map1a	UTSW	2	121,131,299 (GRCm39)	missense	probably damaging	1.00
R7305:Map1a	UTSW	2	121,129,939 (GRCm39)	missense	probably damaging	1.00
R7382:Map1a	UTSW	2	121,121,266 (GRCm39)	missense	probably damaging	1.00
R7524:Map1a	UTSW	2	121,120,293 (GRCm39)	missense	probably damaging	1.00
R7699:Map1a	UTSW	2	121,130,201 (GRCm39)	missense	probably damaging	1.00
R7767:Map1a	UTSW	2	121,132,517 (GRCm39)	missense	probably damaging	1.00
R7883:Map1a	UTSW	2	121,135,853 (GRCm39)	missense	probably damaging	1.00
R7896:Map1a	UTSW	2	121,135,657 (GRCm39)	missense	probably benign	0.00
R7993:Map1a	UTSW	2	121,135,057 (GRCm39)	missense	possibly damaging	0.84
R8270:Map1a	UTSW	2	121,129,501 (GRCm39)	missense	probably damaging	0.99
R8365:Map1a	UTSW	2	121,138,528 (GRCm39)	missense	probably damaging	1.00
R8428:Map1a	UTSW	2	121,135,418 (GRCm39)	missense	probably benign	0.42
R8490:Map1a	UTSW	2	121,135,045 (GRCm39)	missense	possibly damaging	0.93
R8678:Map1a	UTSW	2	121,137,737 (GRCm39)	missense	probably damaging	1.00
R8798:Map1a	UTSW	2	121,132,768 (GRCm39)	missense	probably benign	0.20
R8857:Map1a	UTSW	2	121,138,098 (GRCm39)	missense	probably damaging	1.00
R8878:Map1a	UTSW	2	121,138,125 (GRCm39)	missense	probably damaging	1.00
R8909:Map1a	UTSW	2	121,129,391 (GRCm39)	missense	probably damaging	0.99
R8917:Map1a	UTSW	2	121,131,791 (GRCm39)	missense	possibly damaging	0.93
R8947:Map1a	UTSW	2	121,135,450 (GRCm39)	missense	probably benign	0.27
R9069:Map1a	UTSW	2	121,134,145 (GRCm39)	missense	probably benign	0.15
R9198:Map1a	UTSW	2	121,133,854 (GRCm39)	missense	probably benign	0.00
R9253:Map1a	UTSW	2	121,132,823 (GRCm39)	missense	probably benign	0.00
R9290:Map1a	UTSW	2	121,131,014 (GRCm39)	missense	probably damaging	1.00
R9300:Map1a	UTSW	2	121,133,446 (GRCm39)	missense	probably damaging	1.00
R9589:Map1a	UTSW	2	121,136,398 (GRCm39)	missense	probably damaging	1.00
R9680:Map1a	UTSW	2	121,132,865 (GRCm39)	missense	probably damaging	1.00
R9792:Map1a	UTSW	2	121,121,304 (GRCm39)	critical splice donor site	probably null
R9793:Map1a	UTSW	2	121,121,304 (GRCm39)	critical splice donor site	probably null
R9795:Map1a	UTSW	2	121,121,304 (GRCm39)	critical splice donor site	probably null
RF003:Map1a	UTSW	2	121,136,777 (GRCm39)	small insertion	probably benign
RF007:Map1a	UTSW	2	121,136,789 (GRCm39)	small insertion	probably benign
RF009:Map1a	UTSW	2	121,136,782 (GRCm39)	small insertion	probably benign
RF010:Map1a	UTSW	2	121,136,799 (GRCm39)	small insertion	probably benign
RF014:Map1a	UTSW	2	121,136,776 (GRCm39)	small insertion	probably benign
RF017:Map1a	UTSW	2	121,136,789 (GRCm39)	small insertion	probably benign
RF024:Map1a	UTSW	2	121,136,788 (GRCm39)	small insertion	probably benign
RF025:Map1a	UTSW	2	121,136,775 (GRCm39)	small insertion	probably benign
RF030:Map1a	UTSW	2	121,136,798 (GRCm39)	small insertion	probably benign
RF030:Map1a	UTSW	2	121,136,792 (GRCm39)	small insertion	probably benign
RF033:Map1a	UTSW	2	121,136,780 (GRCm39)	small insertion	probably benign
RF034:Map1a	UTSW	2	121,136,788 (GRCm39)	small insertion	probably benign
RF034:Map1a	UTSW	2	121,136,785 (GRCm39)	small insertion	probably benign
RF035:Map1a	UTSW	2	121,136,782 (GRCm39)	small insertion	probably benign
RF037:Map1a	UTSW	2	121,136,775 (GRCm39)	small insertion	probably benign
RF039:Map1a	UTSW	2	121,136,785 (GRCm39)	small insertion	probably benign
RF042:Map1a	UTSW	2	121,136,768 (GRCm39)	small insertion	probably benign
RF044:Map1a	UTSW	2	121,136,774 (GRCm39)	small insertion	probably benign
RF045:Map1a	UTSW	2	121,136,774 (GRCm39)	small insertion	probably benign
RF051:Map1a	UTSW	2	121,136,777 (GRCm39)	small insertion	probably benign
RF052:Map1a	UTSW	2	121,136,776 (GRCm39)	small insertion	probably benign
RF053:Map1a	UTSW	2	121,136,771 (GRCm39)	small insertion	probably benign
RF060:Map1a	UTSW	2	121,136,799 (GRCm39)	small insertion	probably benign
RF061:Map1a	UTSW	2	121,136,768 (GRCm39)	small insertion	probably benign
Z1176:Map1a	UTSW	2	121,133,719 (GRCm39)	missense	possibly damaging	0.95
Z1177:Map1a	UTSW	2	121,135,760 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- AAGACAGGCATAGTGCTGGC -3'
(R):5'- GACTTGGCTAACTCCTTGGC -3'

Sequencing Primer
(F):5'- CAGGCATAGTGCTGGCCAATG -3'
(R):5'- AACTCCTTGGCCAGCTCTGAG -3'

Posted On

2014-10-15