Mutagenetix > Incidental Mutation

Incidental Mutation 'R2247:Mslnl'

240917

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

040247-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2247 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25955016-25967304 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25961908 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsbg3	A	G	17: 57,184,435 (GRCm39)	E70G	possibly damaging	Het
Ash1l	C	A	3: 88,914,674 (GRCm39)	P1768Q	possibly damaging	Het
Bicra	C	A	7: 15,723,159 (GRCm39)	E119D	probably benign	Het
Brd2	A	G	17: 34,333,389 (GRCm39)	F421L	probably damaging	Het
Cspp1	A	T	1: 10,136,685 (GRCm39)	Q182L	possibly damaging	Het
Dnah7b	A	G	1: 46,316,223 (GRCm39)	D3112G	probably damaging	Het
Dsc2	T	A	18: 20,168,369 (GRCm39)	I697F	probably damaging	Het
Ephb1	A	T	9: 101,874,010 (GRCm39)	S555T	probably damaging	Het
Fat4	A	T	3: 38,946,198 (GRCm39)	D1697V	probably damaging	Het
Hivep2	C	A	10: 14,004,713 (GRCm39)	T437K	probably benign	Het
Impg2	A	G	16: 56,088,627 (GRCm39)	K1160R	probably damaging	Het
Jak2	T	C	19: 29,261,036 (GRCm39)	V344A	probably benign	Het
Kcnab3	A	G	11: 69,221,016 (GRCm39)	T168A	probably damaging	Het
Kctd18	A	T	1: 58,006,801 (GRCm39)	H16Q	possibly damaging	Het
Myh11	A	G	16: 14,095,423 (GRCm39)	L113P	probably damaging	Het
Myh13	A	T	11: 67,225,384 (GRCm39)	I250F	probably damaging	Het
Nin	T	C	12: 70,101,319 (GRCm39)	Y426C	probably damaging	Het
Or10ab4	C	T	7: 107,654,989 (GRCm39)	R267C	probably benign	Het
Or51ab3	T	A	7: 103,201,097 (GRCm39)		probably null	Het
Pbrm1	A	G	14: 30,796,850 (GRCm39)	H897R	probably damaging	Het
Pgap4	T	C	4: 49,586,209 (GRCm39)	S320G	probably benign	Het
Plcl2	T	C	17: 50,913,873 (GRCm39)	V294A	probably damaging	Het
Psapl1	A	G	5: 36,362,410 (GRCm39)	E334G	probably benign	Het
Pwwp3a	A	G	10: 80,076,259 (GRCm39)	Y483C	probably damaging	Het
Raly	A	G	2: 154,705,953 (GRCm39)	H277R	possibly damaging	Het
Ranbp9	T	C	13: 43,565,901 (GRCm39)	K462E	probably damaging	Het
Rptn	A	G	3: 93,304,136 (GRCm39)	T490A	probably benign	Het
Rtl1	T	C	12: 109,561,413 (GRCm39)	H142R	possibly damaging	Het
Scart2	G	A	7: 139,829,042 (GRCm39)	G234S	probably null	Het
Shisa3	G	A	5: 67,768,666 (GRCm39)	V189M	probably benign	Het
Slc25a36	A	T	9: 96,982,191 (GRCm39)	L28Q	probably damaging	Het
Slc38a8	A	G	8: 120,212,389 (GRCm39)	M318T	probably benign	Het
Slc4a11	A	T	2: 130,529,721 (GRCm39)	M328K	probably benign	Het
Sp2	A	C	11: 96,852,844 (GRCm39)		probably null	Het
Sult2b1	A	T	7: 45,384,734 (GRCm39)	I114N	probably damaging	Het
Synrg	T	C	11: 83,900,202 (GRCm39)	S803P	probably damaging	Het
Tenm4	T	A	7: 96,555,216 (GRCm39)	D2603E	probably benign	Het
Tnfaip8	ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	18: 50,179,912 (GRCm39)		probably null	Het
Zfp189	T	A	4: 49,530,393 (GRCm39)	C499S	possibly damaging	Het
Zfp236	A	G	18: 82,622,423 (GRCm39)	F1621S	possibly damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,962,641 (GRCm39)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,963,749 (GRCm39)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,965,125 (GRCm39)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,966,972 (GRCm39)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,963,077 (GRCm39)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,963,051 (GRCm39)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,962,177 (GRCm39)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,961,939 (GRCm39)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,963,491 (GRCm39)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,963,943 (GRCm39)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,957,952 (GRCm39)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,957,942 (GRCm39)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,965,139 (GRCm39)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,962,133 (GRCm39)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,956,816 (GRCm39)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,965,749 (GRCm39)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,956,876 (GRCm39)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,963,531 (GRCm39)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,965,047 (GRCm39)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,962,186 (GRCm39)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,962,184 (GRCm39)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,955,895 (GRCm39)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,962,157 (GRCm39)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,965,751 (GRCm39)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,965,962 (GRCm39)	missense	probably benign
R8735:Mslnl	UTSW	17	25,964,062 (GRCm39)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,964,047 (GRCm39)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,961,694 (GRCm39)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,961,506 (GRCm39)	intron	probably benign
RF007:Mslnl	UTSW	17	25,962,202 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CCATCAGAAGGGAGGTTTGAAC -3'
(R):5'- CACAGGTCTGAAGCACTGAG -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2014-10-15