Mutagenetix > Incidental Mutation

Incidental Mutation 'R2218:Gm7535'

241289

Institutional Source

Beutler Lab

Gene Symbol

Gm7535

Ensembl Gene

ENSMUSG00000090957

Gene Name

predicted gene 7535

Synonyms

MMRRC Submission

040220-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.197) question?

Stock #

R2218 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

18131304-18132987 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

A to G at 18131936 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000093316 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054871] [ENSMUST00000164167]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000054871

SMART Domains

Protein: ENSMUSP00000093316
Gene: ENSMUSG00000079700

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	302	2.2e-43	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000164167

SMART Domains

Protein: ENSMUSP00000125897
Gene: ENSMUSG00000090957

Domain	Start	End	E-Value	Type
Pfam:CNDH2_C	15	292	6.7e-90	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000231820

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abitram	G	T	4: 56,802,693 (GRCm39)	V26L	probably damaging	Het
Acap3	A	G	4: 155,988,319 (GRCm39)		probably null	Het
Appl2	G	T	10: 83,444,601 (GRCm39)	F472L	possibly damaging	Het
Atp13a5	A	T	16: 29,140,464 (GRCm39)	V319D	probably damaging	Het
Atxn2	T	C	5: 121,941,140 (GRCm39)	Y56H	probably damaging	Het
Brinp1	A	G	4: 68,680,952 (GRCm39)	L526P	probably damaging	Het
Cacna1d	C	T	14: 29,845,048 (GRCm39)	D679N	probably damaging	Het
Canx	C	T	11: 50,201,694 (GRCm39)	V59I	probably benign	Het
Drd2	T	C	9: 49,311,094 (GRCm39)	V115A	probably damaging	Het
Eml6	T	A	11: 29,768,907 (GRCm39)	Q746L	probably damaging	Het
F13b	G	T	1: 139,434,582 (GRCm39)	S116I	probably benign	Het
Flt4	T	A	11: 49,515,555 (GRCm39)	S48T	probably benign	Het
Gcn1	C	A	5: 115,757,720 (GRCm39)	S2475Y	probably benign	Het
Gls2	T	C	10: 128,040,583 (GRCm39)	L328P	probably damaging	Het
Htr3a	T	C	9: 48,819,911 (GRCm39)	Y73C	probably damaging	Het
Iapp	G	A	6: 142,249,096 (GRCm39)	A50T	probably benign	Het
Il10ra	T	C	9: 45,176,914 (GRCm39)	D137G	probably benign	Het
Krt35	T	C	11: 99,986,988 (GRCm39)	S9G	probably null	Het
Lamc2	T	A	1: 153,006,525 (GRCm39)	R875S	probably benign	Het
Mcoln1	C	A	8: 3,555,813 (GRCm39)	T36K	possibly damaging	Het
Muc6	T	C	7: 141,233,227 (GRCm39)	H885R	probably benign	Het
Nsrp1	A	T	11: 76,936,587 (GRCm39)	Y536*	probably null	Het
Or10al4	A	T	17: 38,037,145 (GRCm39)	I77F	probably damaging	Het
Polr2a	A	G	11: 69,633,511 (GRCm39)		probably null	Het
Pp2d1	C	A	17: 53,822,482 (GRCm39)	V195L	probably benign	Het
Rag1	T	C	2: 101,474,491 (GRCm39)	H217R	probably benign	Het
Ramp2	A	G	11: 101,138,457 (GRCm39)	E86G	probably benign	Het
Rcbtb2	T	C	14: 73,416,005 (GRCm39)		probably null	Het
Sema5a	A	T	15: 32,631,455 (GRCm39)	I613F	probably damaging	Het
Sgk1	C	T	10: 21,872,500 (GRCm39)	R171W	probably damaging	Het
Slc39a11	C	A	11: 113,450,376 (GRCm39)		probably null	Het
Slc47a2	T	C	11: 61,204,497 (GRCm39)	T285A	probably benign	Het
Timd2	T	A	11: 46,577,844 (GRCm39)	I96L	probably damaging	Het
Tkt	G	T	14: 30,289,018 (GRCm39)		probably null	Het
Tle1	A	T	4: 72,117,556 (GRCm39)	F35I	possibly damaging	Het
Tmem64	T	C	4: 15,266,658 (GRCm39)	I236T	possibly damaging	Het
Ttll13	A	G	7: 79,902,250 (GRCm39)	K109R	probably damaging	Het
Virma	T	C	4: 11,544,924 (GRCm39)	S1628P	probably damaging	Het
Zan	C	A	5: 137,408,568 (GRCm39)		probably benign	Het
Zbtb22	T	G	17: 34,136,939 (GRCm39)	D361E	probably damaging	Het
Zfp608	T	C	18: 55,120,756 (GRCm39)	N277S	probably benign	Het

Other mutations in Gm7535

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL00422:Gm7535	APN	17	18,132,150 (GRCm39)	intron	probably benign
IGL01663:Gm7535	APN	17	18,131,619 (GRCm39)	intron	probably benign
R0165:Gm7535	UTSW	17	18,131,437 (GRCm39)	intron	probably benign
R0335:Gm7535	UTSW	17	18,131,374 (GRCm39)	intron	probably benign
R1985:Gm7535	UTSW	17	18,131,800 (GRCm39)	intron	probably benign
R2217:Gm7535	UTSW	17	18,131,936 (GRCm39)	intron	probably benign
R4464:Gm7535	UTSW	17	18,131,924 (GRCm39)	intron	probably benign
R4581:Gm7535	UTSW	17	18,131,345 (GRCm39)	intron	probably benign
R4887:Gm7535	UTSW	17	18,131,333 (GRCm39)	intron	probably benign
R5225:Gm7535	UTSW	17	18,131,809 (GRCm39)	intron	probably benign
R5305:Gm7535	UTSW	17	18,132,061 (GRCm39)	intron	probably benign
R5641:Gm7535	UTSW	17	18,131,788 (GRCm39)	intron	probably benign
R5658:Gm7535	UTSW	17	18,131,582 (GRCm39)	intron	probably benign
R5760:Gm7535	UTSW	17	18,132,080 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- TCTGCATCAAGGTCAGCAGC -3'
(R):5'- ATCTAAGGTCCTCCAGAAAGGG -3'

Sequencing Primer
(F):5'- AAAGTCATCTGCTACCCC -3'
(R):5'- GGAAACCCTATTCTGTGCCAC -3'

Posted On

2014-10-15