Mutagenetix > Incidental Mutation

Incidental Mutation 'R2221:Frem2'

241474

Institutional Source

Beutler Lab

Gene Symbol

Frem2

Ensembl Gene

ENSMUSG00000037016

Gene Name

Fras1 related extracellular matrix protein 2

Synonyms

8430406N05Rik, ne, 6030440P17Rik, b2b1562Clo, my

MMRRC Submission

040223-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2221 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

53421359-53564776 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 53424278 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 3053 (A3053V)

Ref Sequence

ENSEMBL: ENSMUSP00000088670 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091137]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000091137
AA Change: A3053V

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000088670
Gene: ENSMUSG00000037016
AA Change: A3053V

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
Pfam:Cadherin_3	249	388	4.3e-9	PFAM
Pfam:Cadherin_3	376	532	3e-34	PFAM
Pfam:Cadherin_3	516	665	7.5e-24	PFAM
Pfam:Cadherin_3	632	798	1.6e-21	PFAM
Pfam:Cadherin_3	763	910	1.2e-25	PFAM
Pfam:Cadherin_3	879	1027	5.1e-18	PFAM
Pfam:Cadherin_3	1015	1159	2.2e-20	PFAM
CA	1202	1293	4.8e-1	SMART
Pfam:Cadherin_3	1392	1503	9.8e-24	PFAM
Pfam:Cadherin_3	1504	1612	6.2e-28	PFAM
Pfam:Cadherin_3	1613	1743	5.3e-20	PFAM
Calx_beta	1748	1847	1.5e-5	SMART
Calx_beta	1860	1971	9.47e-12	SMART
Calx_beta	1985	2092	1.65e-11	SMART
Calx_beta	2105	2209	1.99e-5	SMART
Calx_beta	2227	2331	6.9e-14	SMART
transmembrane domain	3103	3125	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000157311

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197519

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199543

Meta Mutation Damage Score

0.0648

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

100% (58/58)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Kidney development is severely affected and syndactyly is common. Phenotypes of homozygous mutants are indistinguishable from those of Fras1 homozygous mutant. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamtsl1	A	C	4: 86,306,762 (GRCm39)	D1392A	probably benign	Het
Afdn	T	C	17: 14,103,999 (GRCm39)		probably benign	Het
Aph1b	A	T	9: 66,691,921 (GRCm39)	M121K	probably damaging	Het
Aspg	G	A	12: 112,080,868 (GRCm39)	A120T	probably damaging	Het
Bicd1	A	G	6: 149,418,503 (GRCm39)	T725A	probably damaging	Het
Cd1d2	A	T	3: 86,895,847 (GRCm39)	I292F	probably damaging	Het
Cdcp3	A	T	7: 130,849,186 (GRCm39)		probably null	Het
Cdk13	A	G	13: 17,894,120 (GRCm39)	V495A	probably damaging	Het
Cfd	A	T	10: 79,728,039 (GRCm39)		probably null	Het
Col9a2	C	G	4: 120,911,455 (GRCm39)	R599G	probably damaging	Het
Cradd	A	C	10: 95,011,735 (GRCm39)	V135G	probably benign	Het
Cry1	A	G	10: 84,979,617 (GRCm39)	C460R	probably damaging	Het
Ctu2	A	G	8: 123,207,649 (GRCm39)	E375G	probably damaging	Het
Dym	T	G	18: 75,363,236 (GRCm39)	I580S	probably damaging	Het
Eif3e	A	G	15: 43,114,943 (GRCm39)	L411P	possibly damaging	Het
Emsy	T	C	7: 98,239,982 (GRCm39)	E1091G	possibly damaging	Het
Enkur	A	G	2: 21,194,130 (GRCm39)		probably benign	Het
Fam184a	G	A	10: 53,531,175 (GRCm39)	T733M	probably damaging	Het
Gbgt1	T	C	2: 28,388,435 (GRCm39)	L40P	probably damaging	Het
Gm5174	T	A	10: 86,492,372 (GRCm39)		noncoding transcript	Het
Gys2	G	T	6: 142,402,148 (GRCm39)	D230E	probably damaging	Het
Herc2	T	A	7: 55,818,766 (GRCm39)		probably null	Het
Hps3	A	T	3: 20,056,527 (GRCm39)	S815R	probably benign	Het
Igf1r	C	A	7: 67,851,710 (GRCm39)	S983R	probably damaging	Het
Itsn1	T	A	16: 91,650,656 (GRCm39)		probably benign	Het
Kcnc2	A	G	10: 112,292,431 (GRCm39)	N91D	probably damaging	Het
Kif28	C	A	1: 179,560,676 (GRCm39)	A210S	possibly damaging	Het
Klri2	T	A	6: 129,717,272 (GRCm39)	Q37L	probably damaging	Het
Lrrc38	T	A	4: 143,096,419 (GRCm39)	C243*	probably null	Het
Map3k5	T	C	10: 19,943,666 (GRCm39)	V590A	possibly damaging	Het
Mdn1	C	A	4: 32,763,306 (GRCm39)	F5135L	probably benign	Het
Megf11	G	A	9: 64,567,713 (GRCm39)	G401S	possibly damaging	Het
Mst1r	T	C	9: 107,785,547 (GRCm39)	F402L	probably damaging	Het
Ntrk3	T	C	7: 77,848,600 (GRCm39)	I759V	probably damaging	Het
Nup188	T	C	2: 30,226,936 (GRCm39)		probably benign	Het
Or4a71	C	T	2: 89,358,281 (GRCm39)	V158I	probably benign	Het
Or8b12i	T	C	9: 20,082,388 (GRCm39)	S160G	possibly damaging	Het
Prdm2	T	C	4: 142,861,469 (GRCm39)	N607S	possibly damaging	Het
Prl2a1	T	A	13: 27,990,369 (GRCm39)		probably null	Het
Serpina9	T	A	12: 103,964,523 (GRCm39)	I305F	probably damaging	Het
Setx	GTGGCT	GT	2: 29,044,073 (GRCm39)	1814	probably null	Het
Slc19a1	C	T	10: 76,878,320 (GRCm39)	T285I	probably benign	Het
Snap91	T	C	9: 86,674,580 (GRCm39)	T544A	possibly damaging	Het
Srgap3	A	G	6: 112,923,454 (GRCm39)	S2P	probably damaging	Het
Tcof1	A	G	18: 60,970,973 (GRCm39)	V210A	possibly damaging	Het
Tex261	A	G	6: 83,748,497 (GRCm39)	I136T	probably benign	Het
Trpa1	A	T	1: 14,973,480 (GRCm39)	F279L	probably null	Het
Ttc39b	T	C	4: 83,150,999 (GRCm39)	N532S	probably benign	Het
Ttn	T	C	2: 76,572,438 (GRCm39)	T26152A	probably damaging	Het
Ube2w	A	G	1: 16,668,183 (GRCm39)	S97P	possibly damaging	Het
Vmn1r25	A	C	6: 57,956,223 (GRCm39)	L22R	probably damaging	Het
Vmn1r64	T	C	7: 5,887,448 (GRCm39)	I32V	probably benign	Het
Vmn2r112	T	G	17: 22,820,214 (GRCm39)	M29R	possibly damaging	Het
Vmn2r71	T	A	7: 85,273,301 (GRCm39)	M705K	probably benign	Het
Vps13b	G	A	15: 35,884,743 (GRCm39)	V3139I	probably benign	Het
Zfp628	T	C	7: 4,923,830 (GRCm39)	V684A	probably benign	Het

Other mutations in Frem2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00895:Frem2	APN	3	53,493,016 (GRCm39)	missense	probably damaging	1.00
IGL00911:Frem2	APN	3	53,479,883 (GRCm39)	missense	probably damaging	1.00
IGL01322:Frem2	APN	3	53,448,459 (GRCm39)	missense	probably benign	0.00
IGL01330:Frem2	APN	3	53,562,662 (GRCm39)	missense	possibly damaging	0.70
IGL01406:Frem2	APN	3	53,433,317 (GRCm39)	missense	probably damaging	1.00
IGL01556:Frem2	APN	3	53,442,702 (GRCm39)	missense	probably benign	0.23
IGL01580:Frem2	APN	3	53,562,596 (GRCm39)	missense	probably damaging	1.00
IGL01606:Frem2	APN	3	53,561,012 (GRCm39)	missense	possibly damaging	0.69
IGL01611:Frem2	APN	3	53,563,130 (GRCm39)	missense	probably benign	0.00
IGL01648:Frem2	APN	3	53,443,153 (GRCm39)	missense	possibly damaging	0.86
IGL01663:Frem2	APN	3	53,424,434 (GRCm39)	missense	probably damaging	1.00
IGL01665:Frem2	APN	3	53,457,083 (GRCm39)	missense	probably benign	0.07
IGL01670:Frem2	APN	3	53,564,358 (GRCm39)	missense	possibly damaging	0.95
IGL01960:Frem2	APN	3	53,429,725 (GRCm39)	missense	probably benign	0.33
IGL02175:Frem2	APN	3	53,563,020 (GRCm39)	missense	possibly damaging	0.69
IGL02201:Frem2	APN	3	53,427,061 (GRCm39)	missense	probably benign	0.35
IGL02202:Frem2	APN	3	53,562,220 (GRCm39)	missense	probably benign	0.00
IGL02427:Frem2	APN	3	53,443,184 (GRCm39)	missense	probably damaging	0.97
IGL02457:Frem2	APN	3	53,428,470 (GRCm39)	missense	probably damaging	0.99
IGL02638:Frem2	APN	3	53,458,767 (GRCm39)	missense	possibly damaging	0.94
IGL02801:Frem2	APN	3	53,559,596 (GRCm39)	missense	possibly damaging	0.85
IGL03023:Frem2	APN	3	53,563,049 (GRCm39)	missense	probably benign	0.40
IGL03169:Frem2	APN	3	53,429,713 (GRCm39)	missense	probably benign	0.01
IGL03238:Frem2	APN	3	53,563,682 (GRCm39)	missense	possibly damaging	0.93
IGL03251:Frem2	APN	3	53,479,729 (GRCm39)	missense	probably benign	0.01
IGL03273:Frem2	APN	3	53,444,930 (GRCm39)	nonsense	probably null
IGL03343:Frem2	APN	3	53,559,674 (GRCm39)	missense	probably damaging	1.00
Biosimilar	UTSW	3	53,561,744 (GRCm39)	missense	probably benign	0.01
Fruit_stripe	UTSW	3	53,444,910 (GRCm39)	missense	probably benign	0.21
PIT4366001:Frem2	UTSW	3	53,560,622 (GRCm39)	missense	probably damaging	0.98
R0019:Frem2	UTSW	3	53,431,099 (GRCm39)	missense	probably damaging	0.99
R0092:Frem2	UTSW	3	53,497,217 (GRCm39)	missense	probably benign	0.03
R0108:Frem2	UTSW	3	53,555,382 (GRCm39)	missense	probably benign	0.03
R0115:Frem2	UTSW	3	53,563,629 (GRCm39)	missense	probably damaging	0.99
R0118:Frem2	UTSW	3	53,442,664 (GRCm39)	nonsense	probably null
R0374:Frem2	UTSW	3	53,561,381 (GRCm39)	missense	probably damaging	1.00
R0437:Frem2	UTSW	3	53,560,436 (GRCm39)	missense	possibly damaging	0.96
R0531:Frem2	UTSW	3	53,427,375 (GRCm39)	missense	probably damaging	1.00
R0555:Frem2	UTSW	3	53,424,281 (GRCm39)	missense	probably damaging	0.97
R0564:Frem2	UTSW	3	53,563,530 (GRCm39)	missense	probably damaging	0.97
R0586:Frem2	UTSW	3	53,555,342 (GRCm39)	missense	probably damaging	0.99
R0726:Frem2	UTSW	3	53,427,047 (GRCm39)	missense	possibly damaging	0.89
R0925:Frem2	UTSW	3	53,561,394 (GRCm39)	missense	probably benign
R1233:Frem2	UTSW	3	53,455,199 (GRCm39)	missense	probably damaging	0.98
R1302:Frem2	UTSW	3	53,562,959 (GRCm39)	missense	probably benign	0.00
R1333:Frem2	UTSW	3	53,457,152 (GRCm39)	missense	probably benign	0.26
R1446:Frem2	UTSW	3	53,562,017 (GRCm39)	missense	probably benign	0.31
R1523:Frem2	UTSW	3	53,562,828 (GRCm39)	missense	possibly damaging	0.73
R1539:Frem2	UTSW	3	53,561,631 (GRCm39)	missense	probably benign	0.19
R1543:Frem2	UTSW	3	53,479,876 (GRCm39)	missense	possibly damaging	0.86
R1597:Frem2	UTSW	3	53,561,940 (GRCm39)	missense	probably benign	0.19
R1600:Frem2	UTSW	3	53,455,144 (GRCm39)	missense	probably damaging	1.00
R1678:Frem2	UTSW	3	53,427,359 (GRCm39)	missense	probably damaging	1.00
R1687:Frem2	UTSW	3	53,561,373 (GRCm39)	missense	probably benign
R1696:Frem2	UTSW	3	53,563,463 (GRCm39)	nonsense	probably null
R1758:Frem2	UTSW	3	53,560,778 (GRCm39)	missense	probably damaging	1.00
R1857:Frem2	UTSW	3	53,562,294 (GRCm39)	missense	probably benign	0.10
R1869:Frem2	UTSW	3	53,442,617 (GRCm39)	missense	probably benign	0.04
R1921:Frem2	UTSW	3	53,560,916 (GRCm39)	missense	possibly damaging	0.76
R1973:Frem2	UTSW	3	53,559,653 (GRCm39)	missense	probably benign	0.01
R2045:Frem2	UTSW	3	53,443,165 (GRCm39)	missense	probably damaging	1.00
R2113:Frem2	UTSW	3	53,560,343 (GRCm39)	missense	probably damaging	1.00
R2152:Frem2	UTSW	3	53,424,450 (GRCm39)	nonsense	probably null
R2164:Frem2	UTSW	3	53,444,751 (GRCm39)	missense	probably damaging	1.00
R2181:Frem2	UTSW	3	53,482,008 (GRCm39)	missense	possibly damaging	0.72
R2201:Frem2	UTSW	3	53,423,994 (GRCm39)	missense	probably benign
R2255:Frem2	UTSW	3	53,559,935 (GRCm39)	missense	probably damaging	0.96
R2280:Frem2	UTSW	3	53,479,844 (GRCm39)	missense	probably damaging	1.00
R3196:Frem2	UTSW	3	53,444,752 (GRCm39)	missense	probably damaging	1.00
R3716:Frem2	UTSW	3	53,479,781 (GRCm39)	missense	probably damaging	1.00
R3807:Frem2	UTSW	3	53,560,870 (GRCm39)	missense	probably benign	0.22
R3820:Frem2	UTSW	3	53,424,270 (GRCm39)	missense	probably damaging	1.00
R3821:Frem2	UTSW	3	53,559,836 (GRCm39)	missense	probably damaging	1.00
R3977:Frem2	UTSW	3	53,559,491 (GRCm39)	missense	probably benign	0.00
R3979:Frem2	UTSW	3	53,559,491 (GRCm39)	missense	probably benign	0.00
R4014:Frem2	UTSW	3	53,559,774 (GRCm39)	missense	probably benign	0.01
R4127:Frem2	UTSW	3	53,433,317 (GRCm39)	missense	probably damaging	1.00
R4195:Frem2	UTSW	3	53,446,689 (GRCm39)	missense	possibly damaging	0.90
R4196:Frem2	UTSW	3	53,446,689 (GRCm39)	missense	possibly damaging	0.90
R4374:Frem2	UTSW	3	53,452,923 (GRCm39)	missense	possibly damaging	0.61
R4427:Frem2	UTSW	3	53,446,583 (GRCm39)	critical splice donor site	probably null
R4428:Frem2	UTSW	3	53,561,759 (GRCm39)	missense	probably benign	0.40
R4559:Frem2	UTSW	3	53,561,742 (GRCm39)	missense	probably benign	0.01
R4600:Frem2	UTSW	3	53,455,228 (GRCm39)	missense	possibly damaging	0.96
R4602:Frem2	UTSW	3	53,455,228 (GRCm39)	missense	possibly damaging	0.96
R4610:Frem2	UTSW	3	53,455,228 (GRCm39)	missense	possibly damaging	0.96
R4611:Frem2	UTSW	3	53,455,228 (GRCm39)	missense	possibly damaging	0.96
R4661:Frem2	UTSW	3	53,562,864 (GRCm39)	missense	probably damaging	1.00
R4678:Frem2	UTSW	3	53,451,792 (GRCm39)	missense	probably benign	0.00
R4689:Frem2	UTSW	3	53,455,056 (GRCm39)	missense	probably benign	0.43
R4740:Frem2	UTSW	3	53,443,240 (GRCm39)	missense	probably benign	0.04
R4748:Frem2	UTSW	3	53,448,514 (GRCm39)	missense	probably damaging	1.00
R4790:Frem2	UTSW	3	53,424,162 (GRCm39)	missense	probably benign
R4809:Frem2	UTSW	3	53,561,316 (GRCm39)	missense	probably benign	0.01
R4930:Frem2	UTSW	3	53,563,736 (GRCm39)	missense	possibly damaging	0.93
R4971:Frem2	UTSW	3	53,446,604 (GRCm39)	missense	probably damaging	1.00
R5057:Frem2	UTSW	3	53,442,617 (GRCm39)	missense	probably benign	0.37
R5202:Frem2	UTSW	3	53,458,767 (GRCm39)	missense	probably benign	0.41
R5221:Frem2	UTSW	3	53,493,032 (GRCm39)	missense	probably damaging	1.00
R5231:Frem2	UTSW	3	53,429,716 (GRCm39)	missense	probably damaging	1.00
R5268:Frem2	UTSW	3	53,560,575 (GRCm39)	missense	probably damaging	0.96
R5480:Frem2	UTSW	3	53,563,928 (GRCm39)	nonsense	probably null
R5637:Frem2	UTSW	3	53,560,358 (GRCm39)	missense	probably damaging	0.97
R5664:Frem2	UTSW	3	53,559,911 (GRCm39)	missense	probably benign	0.33
R5698:Frem2	UTSW	3	53,559,926 (GRCm39)	missense	possibly damaging	0.89
R5744:Frem2	UTSW	3	53,563,380 (GRCm39)	missense	probably damaging	1.00
R5754:Frem2	UTSW	3	53,444,679 (GRCm39)	missense	probably damaging	1.00
R5808:Frem2	UTSW	3	53,559,984 (GRCm39)	missense	probably damaging	0.96
R5840:Frem2	UTSW	3	53,555,342 (GRCm39)	missense	probably damaging	0.99
R5874:Frem2	UTSW	3	53,444,910 (GRCm39)	missense	probably benign	0.21
R6050:Frem2	UTSW	3	53,560,433 (GRCm39)	missense	probably damaging	0.99
R6103:Frem2	UTSW	3	53,457,209 (GRCm39)	missense	probably benign	0.00
R6149:Frem2	UTSW	3	53,458,762 (GRCm39)	missense	probably damaging	0.98
R6182:Frem2	UTSW	3	53,555,390 (GRCm39)	missense	probably damaging	1.00
R6191:Frem2	UTSW	3	53,562,701 (GRCm39)	missense	probably benign	0.10
R6245:Frem2	UTSW	3	53,563,245 (GRCm39)	missense	probably benign	0.00
R6252:Frem2	UTSW	3	53,479,869 (GRCm39)	missense	probably damaging	1.00
R6393:Frem2	UTSW	3	53,493,061 (GRCm39)	missense	possibly damaging	0.91
R6416:Frem2	UTSW	3	53,479,799 (GRCm39)	missense	probably benign	0.01
R6595:Frem2	UTSW	3	53,457,205 (GRCm39)	missense	probably damaging	1.00
R6665:Frem2	UTSW	3	53,562,077 (GRCm39)	missense	probably damaging	1.00
R6708:Frem2	UTSW	3	53,492,922 (GRCm39)	missense	probably benign	0.00
R6751:Frem2	UTSW	3	53,561,086 (GRCm39)	missense	probably damaging	1.00
R6787:Frem2	UTSW	3	53,561,744 (GRCm39)	missense	probably benign	0.01
R6913:Frem2	UTSW	3	53,424,242 (GRCm39)	missense	probably damaging	1.00
R6916:Frem2	UTSW	3	53,455,109 (GRCm39)	missense	probably damaging	1.00
R7017:Frem2	UTSW	3	53,427,023 (GRCm39)	missense	probably benign	0.02
R7083:Frem2	UTSW	3	53,444,914 (GRCm39)	missense	probably damaging	0.99
R7108:Frem2	UTSW	3	53,560,934 (GRCm39)	missense	probably damaging	1.00
R7133:Frem2	UTSW	3	53,479,760 (GRCm39)	missense	possibly damaging	0.82
R7326:Frem2	UTSW	3	53,562,174 (GRCm39)	missense	probably damaging	1.00
R7341:Frem2	UTSW	3	53,561,916 (GRCm39)	missense	probably damaging	1.00
R7455:Frem2	UTSW	3	53,479,701 (GRCm39)	splice site	probably null
R7487:Frem2	UTSW	3	53,561,970 (GRCm39)	missense	probably benign	0.40
R7495:Frem2	UTSW	3	53,424,258 (GRCm39)	missense	probably benign	0.13
R7542:Frem2	UTSW	3	53,560,000 (GRCm39)	missense	probably damaging	1.00
R7636:Frem2	UTSW	3	53,560,668 (GRCm39)	missense	probably benign	0.00
R7703:Frem2	UTSW	3	53,429,589 (GRCm39)	missense	probably benign	0.01
R7750:Frem2	UTSW	3	53,431,103 (GRCm39)	missense	possibly damaging	0.83
R7849:Frem2	UTSW	3	53,479,795 (GRCm39)	missense	probably damaging	1.00
R7922:Frem2	UTSW	3	53,560,725 (GRCm39)	missense	probably damaging	0.98
R8008:Frem2	UTSW	3	53,560,331 (GRCm39)	missense	probably damaging	1.00
R8051:Frem2	UTSW	3	53,442,776 (GRCm39)	missense	probably benign	0.04
R8052:Frem2	UTSW	3	53,457,064 (GRCm39)	missense	probably benign	0.02
R8176:Frem2	UTSW	3	53,562,761 (GRCm39)	missense	possibly damaging	0.50
R8220:Frem2	UTSW	3	53,563,928 (GRCm39)	nonsense	probably null
R8397:Frem2	UTSW	3	53,560,562 (GRCm39)	missense	probably benign	0.00
R8410:Frem2	UTSW	3	53,446,598 (GRCm39)	missense	possibly damaging	0.60
R8697:Frem2	UTSW	3	53,433,249 (GRCm39)	missense	probably damaging	0.99
R9134:Frem2	UTSW	3	53,562,321 (GRCm39)	missense	probably damaging	1.00
R9183:Frem2	UTSW	3	53,427,486 (GRCm39)	missense	probably damaging	1.00
R9260:Frem2	UTSW	3	53,560,204 (GRCm39)	missense	probably damaging	1.00
R9267:Frem2	UTSW	3	53,564,504 (GRCm39)	start codon destroyed	probably null	0.00
R9299:Frem2	UTSW	3	53,563,980 (GRCm39)	missense	probably benign	0.37
R9378:Frem2	UTSW	3	53,559,410 (GRCm39)	missense	probably damaging	0.99
R9444:Frem2	UTSW	3	53,560,265 (GRCm39)	missense	probably benign	0.10
R9459:Frem2	UTSW	3	53,560,907 (GRCm39)	missense	probably benign
R9487:Frem2	UTSW	3	53,560,905 (GRCm39)	missense	possibly damaging	0.95
R9728:Frem2	UTSW	3	53,564,052 (GRCm39)	missense	probably benign	0.00
R9759:Frem2	UTSW	3	53,562,918 (GRCm39)	missense	possibly damaging	0.76
Z1177:Frem2	UTSW	3	53,563,028 (GRCm39)	missense	probably benign	0.31
Z1177:Frem2	UTSW	3	53,442,587 (GRCm39)	missense	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- GAGTATCCTTGCCCTTAGTGC -3'
(R):5'- GCCGAGAGTGGTACATACAC -3'

Sequencing Primer
(F):5'- TGTTCCTGCACATGACGG -3'
(R):5'- GTGGTACATACACACAATCTATACGG -3'

Posted On

2014-10-15