Mutagenetix > Incidental Mutation

Incidental Mutation 'R0172:Or2a25'

24271

Institutional Source

Beutler Lab

Gene Symbol

Or2a25

Ensembl Gene

ENSMUSG00000045708

Gene Name

olfactory receptor family 2 subfamily A member 25

Synonyms

GA_x6K02T2P3E9-4647978-4647046, MOR261-1, Olfr447

MMRRC Submission

038444-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.226) question?

Stock #

R0172 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

42888459-42889391 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 42888913 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 152 (V152A)

Ref Sequence

ENSEMBL: ENSMUSP00000149325 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055763] [ENSMUST00000216408]

AlphaFold

Q8VGP6

Predicted Effect

probably benign
Transcript: ENSMUST00000055763
AA Change: V152A

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000052312
Gene: ENSMUSG00000045708
AA Change: V152A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	307	1.8e-60	PFAM
Pfam:7tm_1	40	289	6.3e-27	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216408
AA Change: V152A

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

Meta Mutation Damage Score

0.0782

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.4%
20x: 93.4%

Validation Efficiency

82% (40/49)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430033K04Rik	T	A	5: 138,645,578 (GRCm39)	C488S	probably damaging	Het
Abca12	T	G	1: 71,318,561 (GRCm39)	D1814A	probably damaging	Het
Acp7	T	C	7: 28,314,549 (GRCm39)	N272S	possibly damaging	Het
Ank3	T	C	10: 69,811,888 (GRCm39)	V1145A	probably damaging	Het
Ap1m2	A	T	9: 21,209,628 (GRCm39)		probably null	Het
Atp12a	T	C	14: 56,610,301 (GRCm39)	V224A	probably damaging	Het
Cdh23	T	C	10: 60,155,411 (GRCm39)	E2253G	probably damaging	Het
Cep350	T	C	1: 155,829,193 (GRCm39)	N237S	probably benign	Het
Crispld2	T	C	8: 120,752,810 (GRCm39)	V286A	possibly damaging	Het
Cyp2c65	G	T	19: 39,076,100 (GRCm39)	V351L	possibly damaging	Het
D130043K22Rik	T	C	13: 25,056,389 (GRCm39)	F574L	probably benign	Het
Dag1	G	A	9: 108,086,031 (GRCm39)	T370M	possibly damaging	Het
Dmwd	C	T	7: 18,814,267 (GRCm39)	R306C	probably damaging	Het
Dnah11	T	C	12: 117,951,188 (GRCm39)	Y3040C	probably damaging	Het
Dst	C	A	1: 34,309,935 (GRCm39)	H1536Q	probably damaging	Het
Eif3j1	A	G	2: 121,882,246 (GRCm39)	I202V	probably benign	Het
Epg5	T	A	18: 78,070,574 (GRCm39)	V2283D	probably benign	Het
Evi5	T	C	5: 107,938,328 (GRCm39)	N625S	probably benign	Het
Exosc10	T	C	4: 148,649,814 (GRCm39)	S415P	probably benign	Het
F830016B08Rik	G	T	18: 60,433,036 (GRCm39)	D40Y	possibly damaging	Het
Fam118a	A	G	15: 84,929,951 (GRCm39)	I60V	probably benign	Het
Fam186a	A	T	15: 99,852,768 (GRCm39)	M150K	unknown	Het
Fam193a	C	T	5: 34,622,957 (GRCm39)	R1182W	probably damaging	Het
Fastkd2	T	A	1: 63,771,187 (GRCm39)	I181K	possibly damaging	Het
Hip1r	T	A	5: 124,135,003 (GRCm39)	Y380N	possibly damaging	Het
Hivep2	C	A	10: 14,015,218 (GRCm39)	P1795Q	probably damaging	Het
Hnrnpab	T	C	11: 51,493,494 (GRCm39)	E238G	probably damaging	Het
Kcnma1	C	T	14: 23,853,234 (GRCm39)	A172T	probably damaging	Het
Lipg	G	T	18: 75,081,245 (GRCm39)	H279N	possibly damaging	Het
Lrrc9	A	G	12: 72,510,260 (GRCm39)	D453G	possibly damaging	Het
Map1s	T	A	8: 71,367,612 (GRCm39)	M839K	probably benign	Het
Miox	C	T	15: 89,220,477 (GRCm39)	L189F	possibly damaging	Het
Myo1h	T	A	5: 114,467,225 (GRCm39)		probably null	Het
Ncoa6	TGC	TGCGC	2: 155,250,211 (GRCm39)		probably null	Het
Nrn1	T	C	13: 36,914,544 (GRCm39)	R19G	probably benign	Het
Nwd2	T	C	5: 63,963,712 (GRCm39)	Y1099H	probably benign	Het
Nxpe2	G	A	9: 48,231,209 (GRCm39)	R387C	possibly damaging	Het
Pappa2	C	T	1: 158,682,419 (GRCm39)		probably null	Het
Pcdhb13	A	T	18: 37,575,990 (GRCm39)	I123L	probably benign	Het
Plcg2	A	G	8: 118,306,521 (GRCm39)	T292A	probably benign	Het
Pnpla8	T	A	12: 44,358,111 (GRCm39)	V469D	probably damaging	Het
Pop4	T	C	7: 37,962,679 (GRCm39)	Y195C	probably damaging	Het
Rbsn	A	T	6: 92,188,588 (GRCm39)	D42E	probably damaging	Het
Sclt1	A	T	3: 41,672,222 (GRCm39)	I123N	possibly damaging	Het
Slc22a27	C	A	19: 7,843,201 (GRCm39)	G393*	probably null	Het
Smu1	C	T	4: 40,738,439 (GRCm39)	V432I	probably benign	Het
Sohlh1	A	G	2: 25,736,215 (GRCm39)		probably null	Het
Spta1	T	A	1: 174,058,352 (GRCm39)	I1940K	probably damaging	Het
Sufu	G	T	19: 46,385,563 (GRCm39)	V8F	possibly damaging	Het
Tmem144	G	A	3: 79,746,580 (GRCm39)		probably benign	Het
Tmem184b	A	G	15: 79,262,740 (GRCm39)	V39A	possibly damaging	Het
Tmem236	A	G	2: 14,223,694 (GRCm39)	D161G	probably benign	Het
Ufl1	T	C	4: 25,280,685 (GRCm39)	K54R	probably benign	Het
Vmn1r28	G	A	6: 58,242,702 (GRCm39)	A182T	probably benign	Het

Other mutations in Or2a25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02302:Or2a25	APN	6	42,889,272 (GRCm39)	missense	probably damaging	1.00
IGL02604:Or2a25	APN	6	42,888,992 (GRCm39)	nonsense	probably null
IGL02894:Or2a25	APN	6	42,888,451 (GRCm39)	splice site	probably benign
R0122:Or2a25	UTSW	6	42,888,889 (GRCm39)	missense	probably benign	0.00
R0371:Or2a25	UTSW	6	42,888,872 (GRCm39)	missense	probably benign	0.14
R1709:Or2a25	UTSW	6	42,889,078 (GRCm39)	missense	possibly damaging	0.94
R2431:Or2a25	UTSW	6	42,888,946 (GRCm39)	missense	probably damaging	0.99
R3809:Or2a25	UTSW	6	42,889,271 (GRCm39)	missense	probably damaging	1.00
R4032:Or2a25	UTSW	6	42,888,559 (GRCm39)	missense	probably benign	0.08
R4242:Or2a25	UTSW	6	42,888,480 (GRCm39)	missense	possibly damaging	0.77
R4369:Or2a25	UTSW	6	42,889,211 (GRCm39)	nonsense	probably null
R4401:Or2a25	UTSW	6	42,889,260 (GRCm39)	nonsense	probably null
R4565:Or2a25	UTSW	6	42,888,472 (GRCm39)	missense	probably benign
R5646:Or2a25	UTSW	6	42,888,457 (GRCm39)	splice site	probably null
R5968:Or2a25	UTSW	6	42,888,480 (GRCm39)	missense	probably benign
R6804:Or2a25	UTSW	6	42,888,852 (GRCm39)	missense	probably benign
R6925:Or2a25	UTSW	6	42,888,791 (GRCm39)	nonsense	probably null
R8170:Or2a25	UTSW	6	42,889,125 (GRCm39)	missense	possibly damaging	0.73
R8358:Or2a25	UTSW	6	42,888,976 (GRCm39)	missense	possibly damaging	0.51
R8765:Or2a25	UTSW	6	42,889,102 (GRCm39)	missense	probably benign	0.34
R9005:Or2a25	UTSW	6	42,889,170 (GRCm39)	missense	probably damaging	1.00
R9015:Or2a25	UTSW	6	42,888,825 (GRCm39)	missense	possibly damaging	0.55
R9308:Or2a25	UTSW	6	42,888,931 (GRCm39)	missense	probably damaging	1.00
R9520:Or2a25	UTSW	6	42,889,051 (GRCm39)	missense	probably damaging	0.98
R9743:Or2a25	UTSW	6	42,888,835 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGACATTGCCTATGCCTGCAACAC -3'
(R):5'- GCCCCTGCTGTGACTTAATCTTCAG -3'

Sequencing Primer
(F):5'- TGCCCCAGATGCTGGTAAAC -3'
(R):5'- TCTTCAGGATGGCACACAG -3'

Posted On

2013-04-16