Incidental Mutation 'R2282:Wnt10b'
ID 243194
Institutional Source Beutler Lab
Gene Symbol Wnt10b
Ensembl Gene ENSMUSG00000022996
Gene Name wingless-type MMTV integration site family, member 10B
Synonyms Wnt12
MMRRC Submission 040281-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R2282 (G1)
Quality Score 225
Status Not validated
Chromosome 15
Chromosomal Location 98668593-98676031 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 98672102 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Serine at position 205 (F205S)
Ref Sequence ENSEMBL: ENSMUSP00000153930 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000023732] [ENSMUST00000166022] [ENSMUST00000226610] [ENSMUST00000226655] [ENSMUST00000226846] [ENSMUST00000228546] [ENSMUST00000228594]
AlphaFold P48614
Predicted Effect probably damaging
Transcript: ENSMUST00000023732
AA Change: F205S

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000023732
Gene: ENSMUSG00000022996
AA Change: F205S

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WNT1 50 389 9.1e-128 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000166022
AA Change: F205S

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000131056
Gene: ENSMUSG00000022996
AA Change: F205S

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WNT1 50 389 9.1e-128 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000226610
AA Change: F205S

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
Predicted Effect probably benign
Transcript: ENSMUST00000226655
Predicted Effect probably benign
Transcript: ENSMUST00000226846
Predicted Effect probably benign
Transcript: ENSMUST00000228546
Predicted Effect probably damaging
Transcript: ENSMUST00000228594
AA Change: F205S

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.2%
  • 20x: 94.9%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice fed a low- or high-fat diet exhibit accelerated myogenic differentiation of myoblasts and those fed a high-fat diet exhibit excessive lipid accumulation in actively regenerating muscle. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 27 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Alpk2 A G 18: 65,440,697 (GRCm39) L232P probably benign Het
Ap5b1 A T 19: 5,619,665 (GRCm39) T362S possibly damaging Het
Ash2l A G 8: 26,325,070 (GRCm39) M163T probably damaging Het
Atp13a5 T C 16: 29,056,073 (GRCm39) Y1029C probably damaging Het
Baz1a G T 12: 54,963,597 (GRCm39) S829* probably null Het
Cd1d2 A G 3: 86,894,558 (GRCm39) K109E probably benign Het
Col4a3 T C 1: 82,686,359 (GRCm39) F1309L unknown Het
Dnah5 A T 15: 28,327,448 (GRCm39) E2055V probably damaging Het
Eif1ad8 A T 12: 87,564,087 (GRCm39) I141F unknown Het
Epha5 A T 5: 84,298,269 (GRCm39) I542N probably damaging Het
Hectd1 A T 12: 51,815,791 (GRCm39) V1432D possibly damaging Het
Klf12 C T 14: 100,137,581 (GRCm39) E322K probably damaging Het
Mslnl G A 17: 25,961,908 (GRCm39) V128M probably damaging Het
Or1j11 G A 2: 36,312,012 (GRCm39) V201I probably benign Het
Or2y1c T G 11: 49,361,470 (GRCm39) M164R probably damaging Het
Or4k5 A G 14: 50,385,776 (GRCm39) V185A probably benign Het
Or8c16 A G 9: 38,130,124 (GRCm39) M2V probably benign Het
Pde6b A G 5: 108,571,452 (GRCm39) probably null Het
Pdzd2 C T 15: 12,373,934 (GRCm39) G2067D possibly damaging Het
Rfx2 T C 17: 57,110,722 (GRCm39) Y88C probably damaging Het
S100a1 A G 3: 90,418,599 (GRCm39) F72L probably damaging Het
Smg5 A G 3: 88,252,705 (GRCm39) M164V probably benign Het
Snx5 T A 2: 144,095,595 (GRCm39) E355D probably benign Het
Tmbim6 T C 15: 99,302,407 (GRCm39) F93S probably damaging Het
Trp53bp1 G A 2: 121,100,754 (GRCm39) Q77* probably null Het
Ttn A G 2: 76,629,882 (GRCm39) V14216A possibly damaging Het
Vmn2r68 C T 7: 84,870,859 (GRCm39) C808Y possibly damaging Het
Other mutations in Wnt10b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01347:Wnt10b APN 15 98,674,826 (GRCm39) utr 5 prime probably benign
R0555:Wnt10b UTSW 15 98,670,818 (GRCm39) splice site probably benign
R1747:Wnt10b UTSW 15 98,672,214 (GRCm39) missense probably benign 0.00
R1751:Wnt10b UTSW 15 98,670,556 (GRCm39) missense probably damaging 0.99
R1767:Wnt10b UTSW 15 98,670,556 (GRCm39) missense probably damaging 0.99
R2272:Wnt10b UTSW 15 98,672,228 (GRCm39) missense probably damaging 0.99
R3911:Wnt10b UTSW 15 98,672,219 (GRCm39) missense possibly damaging 0.53
R4997:Wnt10b UTSW 15 98,672,084 (GRCm39) missense probably damaging 0.99
R5226:Wnt10b UTSW 15 98,674,495 (GRCm39) missense probably damaging 1.00
R7514:Wnt10b UTSW 15 98,672,045 (GRCm39) missense probably benign 0.28
R8516:Wnt10b UTSW 15 98,670,761 (GRCm39) missense probably damaging 0.99
R9551:Wnt10b UTSW 15 98,670,713 (GRCm39) missense probably damaging 1.00
R9552:Wnt10b UTSW 15 98,670,713 (GRCm39) missense probably damaging 1.00
R9617:Wnt10b UTSW 15 98,674,609 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- ATTACTCTGTGGGGCCTCTC -3'
(R):5'- TCATGCATGCTGTTGCCAC -3'

Sequencing Primer
(F):5'- TCTGAGAAAAGCCTGGCTC -3'
(R):5'- TGTTGCCACAGCCTGCAG -3'
Posted On 2014-10-16