Mutagenetix > Incidental Mutation

Incidental Mutation 'R2290:Med12l'

244245

Institutional Source

Beutler Lab

Gene Symbol

Med12l

Ensembl Gene

ENSMUSG00000056476

Gene Name

mediator complex subunit 12-like

Synonyms

MMRRC Submission

040289-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.308) question?

Stock #

R2290 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

58913246-59226103 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 59152359 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 1048 (N1048S)

Ref Sequence

ENSEMBL: ENSMUSP00000127038 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040325] [ENSMUST00000050360] [ENSMUST00000164225] [ENSMUST00000199609] [ENSMUST00000199659]

AlphaFold

Q8BQM9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000040325
AA Change: N1013S

PolyPhen 2 Score 0.912 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000042269
Gene: ENSMUSG00000056476
AA Change: N1013S

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	730	2.6e-207	PFAM
low complexity region	744	758	N/A	INTRINSIC
low complexity region	853	872	N/A	INTRINSIC
low complexity region	1455	1466	N/A	INTRINSIC
low complexity region	1728	1742	N/A	INTRINSIC
low complexity region	1769	1783	N/A	INTRINSIC
Pfam:Med12-PQL	1803	2029	2.3e-14	PFAM
low complexity region	2055	2076	N/A	INTRINSIC
low complexity region	2083	2101	N/A	INTRINSIC
low complexity region	2116	2136	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000050360

SMART Domains

Protein: ENSMUSP00000051353
Gene: ENSMUSG00000036353

Domain	Start	End	E-Value	Type
Pfam:7tm_1	48	304	1.3e-40	PFAM
low complexity region	322	335	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164225
AA Change: N1048S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000127038
Gene: ENSMUSG00000056476
AA Change: N1048S

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	283	765	5e-187	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1763	1777	N/A	INTRINSIC
low complexity region	1804	1818	N/A	INTRINSIC
Pfam:Med12-PQL	1840	2063	9.7e-66	PFAM
low complexity region	2090	2111	N/A	INTRINSIC
low complexity region	2118	2136	N/A	INTRINSIC
low complexity region	2151	2171	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000199609

SMART Domains

Protein: ENSMUSP00000143521
Gene: ENSMUSG00000036353

Domain	Start	End	E-Value	Type
Pfam:7tm_1	23	304	1.5e-31	PFAM
low complexity region	322	335	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000199659
AA Change: N1048S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000142903
Gene: ENSMUSG00000056476
AA Change: N1048S

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	765	5.5e-209	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1761	1775	N/A	INTRINSIC
low complexity region	1802	1816	N/A	INTRINSIC
Pfam:Med12-PQL	1836	2062	1.7e-15	PFAM
low complexity region	2088	2130	N/A	INTRINSIC
low complexity region	2144	2164	N/A	INTRINSIC

Meta Mutation Damage Score

0.3047

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

100% (73/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery. [provided by RefSeq, May 2010]

Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aldh1l2	T	C	10: 83,363,177 (GRCm39)	D62G	probably damaging	Het
Ambp	T	C	4: 63,061,924 (GRCm39)	Y335C	probably damaging	Het
Arid4a	G	T	12: 71,108,315 (GRCm39)	G40V	probably damaging	Het
Asb13	G	A	13: 3,699,418 (GRCm39)	G206D	probably damaging	Het
Ccdc7b	A	G	8: 129,857,587 (GRCm39)		probably benign	Het
Cdh15	A	T	8: 123,586,056 (GRCm39)	N145I	probably damaging	Het
Celsr3	T	C	9: 108,720,423 (GRCm39)	I2565T	probably damaging	Het
Cfap43	A	T	19: 47,761,574 (GRCm39)	M840K	probably damaging	Het
Clip4	T	C	17: 72,117,948 (GRCm39)	V331A	possibly damaging	Het
Cnnm1	A	G	19: 43,479,941 (GRCm39)	T829A	probably benign	Het
Col24a1	G	A	3: 145,218,950 (GRCm39)	G1457E	probably damaging	Het
D6Ertd527e	A	G	6: 87,088,527 (GRCm39)	N230S	unknown	Het
Dll1	T	C	17: 15,595,010 (GRCm39)	D89G	probably benign	Het
Dst	T	A	1: 34,268,281 (GRCm39)	V2901E	probably damaging	Het
Dvl1	G	A	4: 155,932,273 (GRCm39)	V28I	possibly damaging	Het
Efl1	A	T	7: 82,426,878 (GRCm39)	K1125N	probably damaging	Het
Efr3a	T	A	15: 65,721,688 (GRCm39)	F437L	probably benign	Het
Eqtn	GTTCTTCTTC	GTTCTTC	4: 94,815,179 (GRCm39)		probably benign	Het
Ermp1	T	C	19: 29,601,178 (GRCm39)	D523G	probably damaging	Het
Gm38999	A	G	7: 43,077,123 (GRCm39)	E5G	probably benign	Het
Gm5828	C	T	1: 16,838,568 (GRCm39)		noncoding transcript	Het
Gnas	T	C	2: 174,141,803 (GRCm39)	F717L	probably benign	Het
H6pd	T	A	4: 150,066,338 (GRCm39)	S683C	probably damaging	Het
Il23r	G	A	6: 67,400,845 (GRCm39)	T495I	probably benign	Het
Itpr2	T	C	6: 146,324,326 (GRCm39)	N135D	probably damaging	Het
Kcns2	T	A	15: 34,838,655 (GRCm39)	L6Q	possibly damaging	Het
Khdrbs3	C	T	15: 68,901,610 (GRCm39)	R132C	probably damaging	Het
Kif2b	A	G	11: 91,466,522 (GRCm39)	V587A	probably benign	Het
Kng1	A	T	16: 22,897,875 (GRCm39)	H425L	possibly damaging	Het
Letm1	A	AG	5: 33,926,859 (GRCm39)		probably null	Het
Lrrc8e	T	C	8: 4,281,770 (GRCm39)	M35T	probably damaging	Het
Mex3c	A	C	18: 73,723,764 (GRCm39)	N619T	probably damaging	Het
Mfsd4b1	G	A	10: 39,879,327 (GRCm39)	T190I	probably damaging	Het
Ncam1	A	G	9: 49,434,951 (GRCm39)		probably benign	Het
Nlrp6	G	A	7: 140,502,076 (GRCm39)	G133S	probably damaging	Het
Oplah	A	G	15: 76,186,925 (GRCm39)	V630A	probably benign	Het
Or1e26	G	T	11: 73,479,745 (GRCm39)	A273D	probably benign	Het
Or2y1	A	G	11: 49,385,857 (GRCm39)	M166V	probably benign	Het
Or5ak24	C	T	2: 85,260,544 (GRCm39)	V210M	possibly damaging	Het
Or8g36	A	G	9: 39,422,974 (GRCm39)	L14P	possibly damaging	Het
Pcnx2	A	T	8: 126,604,334 (GRCm39)		probably benign	Het
Pkhd1l1	A	T	15: 44,391,646 (GRCm39)	T1571S	probably benign	Het
Pramel13	T	A	4: 144,121,269 (GRCm39)	T252S	probably benign	Het
Pramel13	G	T	4: 144,121,692 (GRCm39)	H111N	probably benign	Het
Prr22	T	G	17: 57,078,884 (GRCm39)	F346V	probably benign	Het
Prr30	T	C	14: 101,436,211 (GRCm39)	N117S	possibly damaging	Het
Ptgfrn	A	G	3: 100,984,677 (GRCm39)	S172P	possibly damaging	Het
Ptprc	C	T	1: 138,038,926 (GRCm39)	V364I	probably benign	Het
Ptprz1	T	C	6: 23,000,990 (GRCm39)	S1027P	probably damaging	Het
Rinl	T	C	7: 28,491,696 (GRCm39)	V83A	probably benign	Het
Ros1	A	T	10: 51,994,477 (GRCm39)	S1268T	probably damaging	Het
Scly	T	C	1: 91,226,172 (GRCm39)		probably null	Het
Slc25a1	C	T	16: 17,743,712 (GRCm39)	V186M	possibly damaging	Het
Stk36	T	G	1: 74,665,303 (GRCm39)		probably benign	Het
Syn2	A	G	6: 115,251,190 (GRCm39)	T449A	possibly damaging	Het
Tecpr1	T	C	5: 144,150,881 (GRCm39)	D309G	probably damaging	Het
Tent4b	T	C	8: 88,978,603 (GRCm39)	S435P	probably damaging	Het
Tns2	T	A	15: 102,020,458 (GRCm39)	Y775N	probably damaging	Het
Tril	G	T	6: 53,795,012 (GRCm39)	R737S	probably damaging	Het
Ttl	G	A	2: 128,923,190 (GRCm39)	G177D	possibly damaging	Het
Twf1	A	G	15: 94,484,400 (GRCm39)	S41P	probably damaging	Het
Unc79	C	T	12: 103,112,625 (GRCm39)	T2174M	probably damaging	Het
Vangl2	T	A	1: 171,836,113 (GRCm39)	K340*	probably null	Het
Vmn2r72	T	A	7: 85,387,549 (GRCm39)	T672S	probably damaging	Het
Vwa7	A	G	17: 35,236,187 (GRCm39)	D47G	probably damaging	Het
Zc3h12d	A	G	10: 7,743,223 (GRCm39)	H331R	probably benign	Het
Zfa-ps	T	C	10: 52,421,112 (GRCm39)		noncoding transcript	Het
Zfp141	A	T	7: 42,124,649 (GRCm39)	C608S	probably damaging	Het
Zfp422	A	G	6: 116,603,603 (GRCm39)	I132T	possibly damaging	Het
Zfp652	A	G	11: 95,640,849 (GRCm39)	Y258C	possibly damaging	Het

Other mutations in Med12l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Med12l	APN	3	58,949,757 (GRCm39)	missense	probably damaging	0.98
IGL00561:Med12l	APN	3	59,135,245 (GRCm39)	missense	probably benign
IGL00974:Med12l	APN	3	58,990,435 (GRCm39)	missense	probably damaging	1.00
IGL01024:Med12l	APN	3	58,980,762 (GRCm39)	missense	probably damaging	1.00
IGL01094:Med12l	APN	3	59,001,076 (GRCm39)	missense	probably damaging	0.99
IGL01134:Med12l	APN	3	58,949,696 (GRCm39)	missense	possibly damaging	0.91
IGL01535:Med12l	APN	3	59,169,680 (GRCm39)	missense	probably damaging	1.00
IGL01653:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL01735:Med12l	APN	3	59,170,675 (GRCm39)	missense	probably damaging	1.00
IGL01972:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL02005:Med12l	APN	3	59,152,368 (GRCm39)	missense	probably damaging	1.00
IGL02098:Med12l	APN	3	59,183,276 (GRCm39)	missense	possibly damaging	0.92
IGL02115:Med12l	APN	3	58,975,740 (GRCm39)	missense	probably benign	0.00
IGL02231:Med12l	APN	3	59,153,303 (GRCm39)	missense	probably damaging	1.00
IGL02259:Med12l	APN	3	59,153,264 (GRCm39)	missense	probably damaging	1.00
IGL02369:Med12l	APN	3	59,164,794 (GRCm39)	missense	probably benign	0.00
IGL02424:Med12l	APN	3	59,000,143 (GRCm39)	missense	probably benign	0.21
IGL02501:Med12l	APN	3	59,169,397 (GRCm39)	missense	possibly damaging	0.71
IGL02525:Med12l	APN	3	58,975,789 (GRCm39)	missense	probably benign	0.01
IGL02530:Med12l	APN	3	58,984,510 (GRCm39)	missense	probably damaging	1.00
IGL02735:Med12l	APN	3	59,001,067 (GRCm39)	missense	probably damaging	1.00
IGL02865:Med12l	APN	3	59,201,713 (GRCm39)	missense	probably damaging	1.00
IGL03183:Med12l	APN	3	58,944,976 (GRCm39)	splice site	probably null
IGL03264:Med12l	APN	3	59,208,788 (GRCm39)	nonsense	probably null
FR4304:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4340:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,415 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,409 (GRCm39)	small insertion	probably benign
FR4449:Med12l	UTSW	3	59,183,384 (GRCm39)	nonsense	probably null
FR4548:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4589:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
FR4976:Med12l	UTSW	3	59,183,398 (GRCm39)	small insertion	probably benign
P0007:Med12l	UTSW	3	58,998,816 (GRCm39)	splice site	probably benign
P0045:Med12l	UTSW	3	58,998,956 (GRCm39)	missense	probably damaging	0.99
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0148:Med12l	UTSW	3	58,945,075 (GRCm39)	missense	probably damaging	1.00
R0325:Med12l	UTSW	3	58,984,480 (GRCm39)	missense	possibly damaging	0.88
R0330:Med12l	UTSW	3	59,135,123 (GRCm39)	missense	probably damaging	1.00
R0388:Med12l	UTSW	3	59,000,925 (GRCm39)	splice site	probably benign
R0542:Med12l	UTSW	3	58,949,822 (GRCm39)	missense	probably damaging	1.00
R0624:Med12l	UTSW	3	58,945,123 (GRCm39)	nonsense	probably null
R0625:Med12l	UTSW	3	59,154,858 (GRCm39)	missense	probably damaging	1.00
R0671:Med12l	UTSW	3	59,172,350 (GRCm39)	missense	probably damaging	1.00
R0706:Med12l	UTSW	3	59,169,401 (GRCm39)	missense	probably damaging	1.00
R0785:Med12l	UTSW	3	59,168,253 (GRCm39)	missense	probably damaging	1.00
R1054:Med12l	UTSW	3	59,156,072 (GRCm39)	missense	probably damaging	0.99
R1102:Med12l	UTSW	3	59,152,257 (GRCm39)	missense	probably damaging	0.99
R1391:Med12l	UTSW	3	58,945,159 (GRCm39)	missense	probably benign	0.00
R1501:Med12l	UTSW	3	59,168,256 (GRCm39)	critical splice donor site	probably null
R1544:Med12l	UTSW	3	59,172,661 (GRCm39)	missense	possibly damaging	0.71
R1662:Med12l	UTSW	3	59,001,038 (GRCm39)	missense	probably damaging	1.00
R1670:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
R1839:Med12l	UTSW	3	58,975,740 (GRCm39)	missense	probably benign
R1854:Med12l	UTSW	3	59,168,193 (GRCm39)	missense	probably damaging	1.00
R2045:Med12l	UTSW	3	59,169,731 (GRCm39)	nonsense	probably null
R2070:Med12l	UTSW	3	59,152,326 (GRCm39)	missense	probably damaging	1.00
R2132:Med12l	UTSW	3	59,172,703 (GRCm39)	splice site	probably null
R2325:Med12l	UTSW	3	59,139,875 (GRCm39)	missense	probably damaging	0.99
R2352:Med12l	UTSW	3	59,148,113 (GRCm39)	missense	probably damaging	1.00
R2484:Med12l	UTSW	3	59,205,259 (GRCm39)	missense	probably benign	0.18
R2906:Med12l	UTSW	3	59,164,503 (GRCm39)	missense	probably damaging	1.00
R3735:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3736:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3774:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R3957:Med12l	UTSW	3	58,980,589 (GRCm39)	missense	probably damaging	0.99
R4020:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R4087:Med12l	UTSW	3	59,205,342 (GRCm39)	missense	probably benign	0.00
R4231:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4233:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4235:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4236:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4327:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4328:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4346:Med12l	UTSW	3	58,938,976 (GRCm39)	missense	probably damaging	1.00
R4543:Med12l	UTSW	3	58,998,929 (GRCm39)	missense	probably damaging	1.00
R4559:Med12l	UTSW	3	58,914,523 (GRCm39)	critical splice donor site	probably null
R4776:Med12l	UTSW	3	59,140,633 (GRCm39)	missense	probably damaging	1.00
R4877:Med12l	UTSW	3	59,152,214 (GRCm39)	missense	probably damaging	1.00
R4983:Med12l	UTSW	3	59,169,350 (GRCm39)	missense	probably damaging	1.00
R5114:Med12l	UTSW	3	59,167,109 (GRCm39)	missense	possibly damaging	0.85
R5125:Med12l	UTSW	3	59,174,635 (GRCm39)	missense	possibly damaging	0.83
R5230:Med12l	UTSW	3	59,153,209 (GRCm39)	missense	probably damaging	1.00
R5407:Med12l	UTSW	3	59,165,622 (GRCm39)	missense	probably damaging	1.00
R5426:Med12l	UTSW	3	59,156,143 (GRCm39)	missense	probably damaging	0.98
R5439:Med12l	UTSW	3	59,170,634 (GRCm39)	missense	probably null	1.00
R5449:Med12l	UTSW	3	59,167,127 (GRCm39)	missense	probably damaging	1.00
R5596:Med12l	UTSW	3	59,159,771 (GRCm39)	missense	probably benign	0.45
R5716:Med12l	UTSW	3	59,208,798 (GRCm39)	critical splice donor site	probably null
R5833:Med12l	UTSW	3	59,172,647 (GRCm39)	missense	possibly damaging	0.95
R5883:Med12l	UTSW	3	58,998,889 (GRCm39)	missense	probably damaging	1.00
R6264:Med12l	UTSW	3	59,163,423 (GRCm39)	missense	probably damaging	1.00
R6269:Med12l	UTSW	3	59,135,243 (GRCm39)	missense	probably damaging	1.00
R6394:Med12l	UTSW	3	59,142,508 (GRCm39)	missense	probably damaging	1.00
R6400:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R6475:Med12l	UTSW	3	59,164,500 (GRCm39)	missense	probably damaging	1.00
R6489:Med12l	UTSW	3	59,164,828 (GRCm39)	missense	probably damaging	0.99
R6654:Med12l	UTSW	3	59,169,713 (GRCm39)	missense	probably damaging	1.00
R6881:Med12l	UTSW	3	59,174,586 (GRCm39)	missense	probably benign	0.00
R7110:Med12l	UTSW	3	59,169,645 (GRCm39)	missense	possibly damaging	0.92
R7134:Med12l	UTSW	3	59,001,180 (GRCm39)	nonsense	probably null
R7137:Med12l	UTSW	3	59,165,675 (GRCm39)	missense	probably damaging	1.00
R7159:Med12l	UTSW	3	59,183,438 (GRCm39)	missense	probably benign
R7341:Med12l	UTSW	3	58,949,824 (GRCm39)	missense	possibly damaging	0.53
R7349:Med12l	UTSW	3	59,165,746 (GRCm39)	missense	probably damaging	1.00
R7413:Med12l	UTSW	3	58,998,971 (GRCm39)	missense	probably benign	0.00
R7495:Med12l	UTSW	3	59,152,194 (GRCm39)	missense	probably damaging	1.00
R7678:Med12l	UTSW	3	58,984,141 (GRCm39)	missense	probably damaging	1.00
R7697:Med12l	UTSW	3	59,148,078 (GRCm39)	missense	probably damaging	1.00
R7714:Med12l	UTSW	3	59,001,007 (GRCm39)	missense	probably benign	0.17
R7725:Med12l	UTSW	3	59,163,413 (GRCm39)	missense	probably damaging	1.00
R7846:Med12l	UTSW	3	59,172,355 (GRCm39)	missense	probably damaging	1.00
R7852:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R8080:Med12l	UTSW	3	59,172,607 (GRCm39)	missense	probably damaging	1.00
R8181:Med12l	UTSW	3	59,169,389 (GRCm39)	missense	probably damaging	1.00
R8223:Med12l	UTSW	3	58,993,784 (GRCm39)	missense	possibly damaging	0.79
R8560:Med12l	UTSW	3	58,945,026 (GRCm39)	missense	probably damaging	1.00
R8708:Med12l	UTSW	3	59,159,751 (GRCm39)	missense	probably benign	0.00
R8865:Med12l	UTSW	3	58,979,303 (GRCm39)	missense	probably benign
R8947:Med12l	UTSW	3	58,984,443 (GRCm39)	splice site	probably benign
R8976:Med12l	UTSW	3	59,183,329 (GRCm39)	missense	probably damaging	0.99
R9016:Med12l	UTSW	3	59,163,294 (GRCm39)	missense	probably damaging	0.96
R9183:Med12l	UTSW	3	58,984,498 (GRCm39)	missense	probably damaging	1.00
R9487:Med12l	UTSW	3	59,155,353 (GRCm39)	missense	probably benign
R9526:Med12l	UTSW	3	58,984,207 (GRCm39)	missense	probably damaging	0.96
R9802:Med12l	UTSW	3	59,169,346 (GRCm39)	missense	probably damaging	1.00
RF004:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF011:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF013:Med12l	UTSW	3	59,183,387 (GRCm39)	small insertion	probably benign
RF020:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
RF021:Med12l	UTSW	3	58,980,711 (GRCm39)	missense	probably benign	0.19
RF027:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF027:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF030:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,408 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF037:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF049:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF050:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF053:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF055:Med12l	UTSW	3	59,183,404 (GRCm39)	small insertion	probably benign
RF056:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF057:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
X0062:Med12l	UTSW	3	59,140,600 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	59,203,538 (GRCm39)	missense	probably benign	0.00
Z1176:Med12l	UTSW	3	59,152,364 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	58,998,838 (GRCm39)	missense	probably damaging	0.98
Z1177:Med12l	UTSW	3	59,155,296 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTGCCTGTTCAAAAGTAAAGC -3'
(R):5'- TGCTTTTCAAAATGGACCACC -3'

Sequencing Primer
(F):5'- TGCCTGTTCAAAAGTAAAGCAGACC -3'
(R):5'- TGTCTACAGCCAAACCAAGAAC -3'

Posted On

2014-10-30