Incidental Mutation 'R2310:Cyp2c65'
ID 244763
Institutional Source Beutler Lab
Gene Symbol Cyp2c65
Ensembl Gene ENSMUSG00000067231
Gene Name cytochrome P450, family 2, subfamily c, polypeptide 65
Synonyms 2210009K14Rik
MMRRC Submission 040309-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.082) question?
Stock # R2310 (G1)
Quality Score 225
Status Validated
Chromosome 19
Chromosomal Location 39049459-39082388 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 39081826 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 451 (T451A)
Ref Sequence ENSEMBL: ENSMUSP00000084489 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000087236]
AlphaFold Q148B1
Predicted Effect probably benign
Transcript: ENSMUST00000087236
AA Change: T451A

PolyPhen 2 Score 0.016 (Sensitivity: 0.95; Specificity: 0.79)
SMART Domains Protein: ENSMUSP00000084489
Gene: ENSMUSG00000067231
AA Change: T451A

DomainStartEndE-ValueType
signal peptide 1 25 N/A INTRINSIC
Pfam:p450 30 487 2.1e-160 PFAM
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.5%
  • 3x: 98.7%
  • 10x: 97.2%
  • 20x: 94.5%
Validation Efficiency 100% (39/39)
Allele List at MGI
Other mutations in this stock
Total: 37 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Arhgef10l A C 4: 140,320,429 (GRCm39) C136W probably damaging Het
Ascc3 T A 10: 50,624,988 (GRCm39) H1625Q probably benign Het
Col1a2 G A 6: 4,518,822 (GRCm39) probably benign Het
Cpa3 C T 3: 20,281,387 (GRCm39) C173Y probably damaging Het
Ddx41 G A 13: 55,682,293 (GRCm39) R205W possibly damaging Het
Efhd1 T C 1: 87,192,350 (GRCm39) L60P probably damaging Het
Flrt2 A G 12: 95,746,864 (GRCm39) T401A probably benign Het
Frmd4a GAA G 2: 4,577,210 (GRCm39) probably null Het
Gas2l2 A G 11: 83,318,265 (GRCm39) V148A possibly damaging Het
Golga5 A G 12: 102,458,420 (GRCm39) E621G probably damaging Het
Gsap A T 5: 21,401,088 (GRCm39) R74* probably null Het
Intu C T 3: 40,608,243 (GRCm39) A85V probably benign Het
Kansl3 T A 1: 36,382,445 (GRCm39) I860F probably damaging Het
Kif23 A G 9: 61,831,426 (GRCm39) S715P probably damaging Het
Map3k8 A T 18: 4,349,001 (GRCm39) C106S probably benign Het
Mcc T C 18: 44,564,433 (GRCm39) E934G probably damaging Het
Mrpl19 A T 6: 81,941,054 (GRCm39) probably null Het
Msl3l2 C A 10: 55,991,421 (GRCm39) R49S probably benign Het
Muc6 T A 7: 141,217,444 (GRCm39) I2410F possibly damaging Het
Or2t6 A G 14: 14,175,836 (GRCm38) V82A probably benign Het
Or4a73 A G 2: 89,420,794 (GRCm39) S222P probably damaging Het
Pip5k1c T G 10: 81,142,142 (GRCm39) S117R probably damaging Het
Ppfia2 T C 10: 106,690,841 (GRCm39) S561P probably damaging Het
Pramel13 A T 4: 144,119,475 (GRCm39) probably null Het
Rassf5 A T 1: 131,172,477 (GRCm39) W131R probably damaging Het
Shq1 C T 6: 100,607,963 (GRCm39) W316* probably null Het
Slc45a4 A G 15: 73,461,409 (GRCm39) Y87H probably damaging Het
Sptbn2 A G 19: 4,768,963 (GRCm39) D32G probably benign Het
Tjp1 C T 7: 64,979,490 (GRCm39) R345Q possibly damaging Het
Tnrc18 C G 5: 142,774,308 (GRCm39) V174L probably damaging Het
Trmt6 G T 2: 132,650,832 (GRCm39) P259T probably damaging Het
Ubap1 T A 4: 41,379,341 (GRCm39) V185E possibly damaging Het
Ulk1 C T 5: 110,937,223 (GRCm39) R691Q probably benign Het
Vmn2r112 T A 17: 22,822,096 (GRCm39) V258E probably damaging Het
Wnk2 T C 13: 49,204,053 (GRCm39) T1979A probably damaging Het
Xirp2 A G 2: 67,356,591 (GRCm39) D3784G probably benign Het
Zfp119a A T 17: 56,172,440 (GRCm39) Y468N probably benign Het
Other mutations in Cyp2c65
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01122:Cyp2c65 APN 19 39,060,621 (GRCm39) critical splice acceptor site probably null
IGL01124:Cyp2c65 APN 19 39,081,954 (GRCm39) utr 3 prime probably benign
IGL01895:Cyp2c65 APN 19 39,060,676 (GRCm39) missense possibly damaging 0.90
IGL02544:Cyp2c65 APN 19 39,079,082 (GRCm39) missense probably damaging 1.00
R0172:Cyp2c65 UTSW 19 39,076,100 (GRCm39) missense possibly damaging 0.86
R0378:Cyp2c65 UTSW 19 39,061,662 (GRCm39) missense probably benign 0.19
R0517:Cyp2c65 UTSW 19 39,070,792 (GRCm39) splice site probably benign
R0585:Cyp2c65 UTSW 19 39,057,686 (GRCm39) missense probably benign 0.00
R1770:Cyp2c65 UTSW 19 39,070,642 (GRCm39) missense probably benign 0.07
R2051:Cyp2c65 UTSW 19 39,070,675 (GRCm39) missense probably benign 0.12
R2911:Cyp2c65 UTSW 19 39,076,126 (GRCm39) missense probably damaging 0.96
R4208:Cyp2c65 UTSW 19 39,079,099 (GRCm39) missense probably damaging 1.00
R4258:Cyp2c65 UTSW 19 39,081,872 (GRCm39) missense probably benign 0.41
R4734:Cyp2c65 UTSW 19 39,060,778 (GRCm39) missense probably benign 0.00
R4821:Cyp2c65 UTSW 19 39,060,635 (GRCm39) missense probably damaging 1.00
R4926:Cyp2c65 UTSW 19 39,049,597 (GRCm39) missense probably benign 0.00
R5060:Cyp2c65 UTSW 19 39,049,514 (GRCm39) missense unknown
R5091:Cyp2c65 UTSW 19 39,076,009 (GRCm39) critical splice acceptor site probably null
R5433:Cyp2c65 UTSW 19 39,081,928 (GRCm39) missense probably benign 0.00
R6051:Cyp2c65 UTSW 19 39,049,610 (GRCm39) missense probably benign 0.29
R6182:Cyp2c65 UTSW 19 39,049,606 (GRCm39) missense probably benign 0.18
R6400:Cyp2c65 UTSW 19 39,049,558 (GRCm39) missense possibly damaging 0.80
R6586:Cyp2c65 UTSW 19 39,070,662 (GRCm39) missense possibly damaging 0.89
R6672:Cyp2c65 UTSW 19 39,076,118 (GRCm39) missense probably damaging 1.00
R6850:Cyp2c65 UTSW 19 39,057,535 (GRCm39) missense probably benign 0.15
R8075:Cyp2c65 UTSW 19 39,060,682 (GRCm39) missense probably benign 0.10
R8756:Cyp2c65 UTSW 19 39,049,552 (GRCm39) nonsense probably null
R9006:Cyp2c65 UTSW 19 39,070,714 (GRCm39) missense probably damaging 1.00
R9031:Cyp2c65 UTSW 19 39,061,663 (GRCm39) nonsense probably null
R9231:Cyp2c65 UTSW 19 39,060,661 (GRCm39) missense possibly damaging 0.92
R9663:Cyp2c65 UTSW 19 39,079,070 (GRCm39) critical splice acceptor site probably null
Predicted Primers PCR Primer
(F):5'- AGTCCTGTGTTCAGTGCTGC -3'
(R):5'- CAGAGAGACATGCCTGACAG -3'

Sequencing Primer
(F):5'- AGTGCTGCTATTTCACCTTCTAAAG -3'
(R):5'- TGCCTGACAGAAGAGGTAAGCTATAC -3'
Posted On 2014-10-30