Incidental Mutation 'R2322:Tcim'
ID 244778
Institutional Source Beutler Lab
Gene Symbol Tcim
Ensembl Gene ENSMUSG00000056313
Gene Name transcriptional and immune response regulator
Synonyms 1810011O10Rik
Accession Numbers
Essential gene? Probably non essential (E-score: 0.220) question?
Stock # R2322 (G1)
Quality Score 225
Status Not validated
Chromosome 8
Chromosomal Location 24927188-24928962 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to C at 24928726 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Glycine at position 63 (R63G)
Ref Sequence ENSEMBL: ENSMUSP00000058631 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000052622]
AlphaFold Q9D915
Predicted Effect probably damaging
Transcript: ENSMUST00000052622
AA Change: R63G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000058631
Gene: ENSMUSG00000056313
AA Change: R63G

DomainStartEndE-ValueType
Pfam:TC1 10 85 5.2e-40 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000181338
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.7%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a small, monomeric, predominantly unstructured protein that functions as a positive regulator of the Wnt/beta-catenin signaling pathway. This protein interacts with a repressor of beta-catenin mediated transcription at nuclear speckles. It is thought to competitively block interactions of the repressor with beta-catenin, resulting in up-regulation of beta-catenin target genes. The encoded protein may also play a role in the NF-kappaB and ERK1/2 signaling pathways. Expression of this gene may play a role in the proliferation of several types of cancer including thyroid cancer, breast cancer and hematological malignancies. [provided by RefSeq, Nov 2011]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit myeloid and lymphoid hyperplasia, an increased number of small-sized red blood cells, increased hematopoietic stem cell number, and enhanced hematopoietic activity. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 21 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A2ml1 C T 6: 128,557,349 (GRCm39) A115T probably benign Het
AY358078 A G 14: 52,042,147 (GRCm39) Y174C unknown Het
Bod1l A T 5: 41,984,463 (GRCm39) V529E probably benign Het
Brinp3 A G 1: 146,577,492 (GRCm39) T176A probably benign Het
Ccn2 T C 10: 24,472,732 (GRCm39) M214T probably damaging Het
Cfap57 T A 4: 118,467,922 (GRCm39) I272L probably benign Het
Cnr1 A G 4: 33,944,514 (GRCm39) K301E probably damaging Het
Elavl1 A T 8: 4,339,802 (GRCm39) L260H probably damaging Het
Ercc6 A G 14: 32,248,274 (GRCm39) Y275C probably damaging Het
Fndc1 A G 17: 8,007,847 (GRCm39) S96P probably damaging Het
Hykk G A 9: 54,853,418 (GRCm39) G247S probably benign Het
Ky T C 9: 102,414,990 (GRCm39) probably null Het
Mcu A C 10: 59,290,766 (GRCm39) probably null Het
Or2y17 T G 11: 49,231,476 (GRCm39) L39R probably damaging Het
Or5w11 T C 2: 87,459,118 (GRCm39) C104R possibly damaging Het
Ppp4r3a C T 12: 101,008,878 (GRCm39) R667Q probably damaging Het
Ptprj T C 2: 90,301,473 (GRCm39) T220A probably benign Het
St18 T A 1: 6,914,348 (GRCm39) C766* probably null Het
St8sia4 C T 1: 95,581,463 (GRCm39) R93H probably damaging Het
Xylb T C 9: 119,217,813 (GRCm39) S488P possibly damaging Het
Zhx3 T C 2: 160,623,948 (GRCm39) D73G probably damaging Het
Other mutations in Tcim
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01386:Tcim APN 8 24,928,705 (GRCm39) missense probably benign 0.04
IGL01937:Tcim APN 8 24,928,892 (GRCm39) missense probably benign
IGL01945:Tcim APN 8 24,928,892 (GRCm39) missense probably benign
R0464:Tcim UTSW 8 24,928,644 (GRCm39) missense probably damaging 1.00
R0519:Tcim UTSW 8 24,928,651 (GRCm39) missense possibly damaging 0.80
R3766:Tcim UTSW 8 24,928,765 (GRCm39) missense probably damaging 0.98
R4623:Tcim UTSW 8 24,928,725 (GRCm39) missense probably damaging 1.00
R6242:Tcim UTSW 8 24,928,911 (GRCm39) start codon destroyed probably null 0.90
R9195:Tcim UTSW 8 24,928,878 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- TCACTGGGTCTTTAAAGGTCC -3'
(R):5'- AACAGACTCCCCTGGAAGTG -3'

Sequencing Primer
(F):5'- CACTGGGTCTTTAAAGGTCCATTGG -3'
(R):5'- AAGTGCCCAAGCTGAGC -3'
Posted On 2014-10-30