Mutagenetix > Incidental Mutation

Incidental Mutation 'R2324:Dpy19l4'

244837

Institutional Source

Beutler Lab

Gene Symbol

Dpy19l4

Ensembl Gene

ENSMUSG00000045205

Gene Name

dpy-19 like 4

Synonyms

Narg3, LOC381510

MMRRC Submission

040315-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R2324 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

11261315-11322137 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to C at 11276857 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000119923 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084892] [ENSMUST00000128024] [ENSMUST00000139385] [ENSMUST00000142005]

AlphaFold

A2AJQ3

Predicted Effect

probably benign
Transcript: ENSMUST00000084892

SMART Domains

Protein: ENSMUSP00000081954
Gene: ENSMUSG00000045205

Domain	Start	End	E-Value	Type
Pfam:Dpy19	59	714	3e-213	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000128024

SMART Domains

Protein: ENSMUSP00000122823
Gene: ENSMUSG00000045205

Domain	Start	End	E-Value	Type
Pfam:Dpy19	58	293	1e-89	PFAM
Pfam:Dpy19	291	524	4.8e-51	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000139385

SMART Domains

Protein: ENSMUSP00000115537
Gene: ENSMUSG00000045205

Domain	Start	End	E-Value	Type
Pfam:Dpy19	1	258	3.2e-71	PFAM
Pfam:Dpy19	254	488	7e-70	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000142005

SMART Domains

Protein: ENSMUSP00000119923
Gene: ENSMUSG00000045205

Domain	Start	End	E-Value	Type
Pfam:Dpy19	58	253	6.9e-77	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000158560

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

100% (33/33)

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akna	T	C	4: 63,290,039 (GRCm39)	D1223G	possibly damaging	Het
Ankrd42	C	A	7: 92,273,186 (GRCm39)	R147L	probably damaging	Het
Cdhr18	A	T	14: 13,868,077 (GRCm38)	I204N	probably damaging	Het
Cep152	T	C	2: 125,405,382 (GRCm39)	I1717V	probably benign	Het
Clca3a2	A	G	3: 144,512,041 (GRCm39)		probably null	Het
Coa8	A	G	12: 111,690,784 (GRCm39)	E112G	possibly damaging	Het
Dnhd1	A	T	7: 105,359,297 (GRCm39)	H3544L	probably damaging	Het
Efcab8	A	G	2: 153,625,729 (GRCm39)		probably null	Het
Esyt2	A	G	12: 116,331,441 (GRCm39)	N773S	possibly damaging	Het
Hectd4	G	A	5: 121,453,500 (GRCm39)	G613D	probably damaging	Het
Ints12	G	A	3: 132,815,126 (GRCm39)	M444I	possibly damaging	Het
Ints3	A	G	3: 90,301,401 (GRCm39)	L827P	probably damaging	Het
Irf9	T	C	14: 55,843,367 (GRCm39)		probably null	Het
Lactb2	T	C	1: 13,708,520 (GRCm39)	D176G	probably damaging	Het
Lmntd2	A	T	7: 140,790,701 (GRCm39)	S516T	possibly damaging	Het
Lrp1	C	A	10: 127,402,455 (GRCm39)	A2239S	possibly damaging	Het
Lrrc56	A	G	7: 140,785,476 (GRCm39)		probably benign	Het
Nfic	A	G	10: 81,241,921 (GRCm39)		probably null	Het
Notch3	G	A	17: 32,369,108 (GRCm39)		probably benign	Het
Oas2	T	C	5: 120,881,339 (GRCm39)	K251E	probably benign	Het
Or5k15	T	C	16: 58,710,503 (GRCm39)	T27A	probably benign	Het
Pabpc4	T	C	4: 123,191,571 (GRCm39)		probably benign	Het
Piwil4	C	T	9: 14,648,204 (GRCm39)	R125H	possibly damaging	Het
Rbsn	A	G	6: 92,170,947 (GRCm39)	Y325H	probably damaging	Het
Rhox2e	C	A	X: 36,712,516 (GRCm39)	P69Q	probably damaging	Het
Skint6	T	C	4: 112,729,654 (GRCm39)		probably null	Het
Tmem165	T	C	5: 76,352,671 (GRCm39)		probably benign	Het
Tmem181a	T	A	17: 6,346,061 (GRCm39)	L185H	probably damaging	Het
Ttc13	A	G	8: 125,405,796 (GRCm39)	V499A	probably damaging	Het
Vax2	G	T	6: 83,688,307 (GRCm39)	R10L	possibly damaging	Het
Zfp988	A	C	4: 147,417,242 (GRCm39)	K559Q	probably benign	Het

Other mutations in Dpy19l4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00479:Dpy19l4	APN	4	11,290,411 (GRCm39)	missense	probably benign	0.00
IGL01402:Dpy19l4	APN	4	11,273,006 (GRCm39)	critical splice donor site	probably null
IGL01404:Dpy19l4	APN	4	11,273,006 (GRCm39)	critical splice donor site	probably null
IGL01643:Dpy19l4	APN	4	11,290,184 (GRCm39)	splice site	probably benign
IGL01758:Dpy19l4	APN	4	11,265,846 (GRCm39)	missense	probably damaging	1.00
IGL01896:Dpy19l4	APN	4	11,267,752 (GRCm39)	missense	possibly damaging	0.81
IGL02222:Dpy19l4	APN	4	11,281,116 (GRCm39)	missense	possibly damaging	0.93
IGL02314:Dpy19l4	APN	4	11,267,720 (GRCm39)	missense	possibly damaging	0.50
IGL02422:Dpy19l4	APN	4	11,265,803 (GRCm39)	missense	possibly damaging	0.95
IGL02565:Dpy19l4	APN	4	11,309,440 (GRCm39)	missense	probably benign	0.14
IGL03121:Dpy19l4	APN	4	11,303,334 (GRCm39)	missense	probably damaging	1.00
IGL03357:Dpy19l4	APN	4	11,267,615 (GRCm39)	missense	probably damaging	1.00
IGL03368:Dpy19l4	APN	4	11,290,253 (GRCm39)	missense	possibly damaging	0.53
R0003:Dpy19l4	UTSW	4	11,267,619 (GRCm39)	missense	probably damaging	1.00
R0481:Dpy19l4	UTSW	4	11,272,993 (GRCm39)	splice site	probably benign
R0506:Dpy19l4	UTSW	4	11,289,715 (GRCm39)	missense	probably benign	0.07
R1114:Dpy19l4	UTSW	4	11,287,643 (GRCm39)	splice site	probably benign
R1332:Dpy19l4	UTSW	4	11,276,901 (GRCm39)	missense	probably damaging	1.00
R1336:Dpy19l4	UTSW	4	11,276,901 (GRCm39)	missense	probably damaging	1.00
R1355:Dpy19l4	UTSW	4	11,303,371 (GRCm39)	nonsense	probably null
R1421:Dpy19l4	UTSW	4	11,304,011 (GRCm39)	missense	probably benign	0.09
R1422:Dpy19l4	UTSW	4	11,317,168 (GRCm39)	missense	possibly damaging	0.88
R1465:Dpy19l4	UTSW	4	11,296,034 (GRCm39)	missense	probably damaging	1.00
R1465:Dpy19l4	UTSW	4	11,296,034 (GRCm39)	missense	probably damaging	1.00
R1766:Dpy19l4	UTSW	4	11,303,360 (GRCm39)	missense	probably damaging	1.00
R1803:Dpy19l4	UTSW	4	11,281,020 (GRCm39)	missense	possibly damaging	0.81
R2090:Dpy19l4	UTSW	4	11,304,344 (GRCm39)	missense	probably benign	0.34
R2446:Dpy19l4	UTSW	4	11,304,143 (GRCm39)	splice site	probably null
R3769:Dpy19l4	UTSW	4	11,276,868 (GRCm39)	splice site	probably null
R4151:Dpy19l4	UTSW	4	11,309,485 (GRCm39)	missense	possibly damaging	0.89
R4472:Dpy19l4	UTSW	4	11,304,053 (GRCm39)	missense	possibly damaging	0.91
R4609:Dpy19l4	UTSW	4	11,295,999 (GRCm39)	nonsense	probably null
R4708:Dpy19l4	UTSW	4	11,277,970 (GRCm39)	missense	probably benign	0.00
R4722:Dpy19l4	UTSW	4	11,290,521 (GRCm39)	missense	possibly damaging	0.84
R4997:Dpy19l4	UTSW	4	11,287,493 (GRCm39)	missense	probably benign	0.01
R5085:Dpy19l4	UTSW	4	11,265,943 (GRCm39)	critical splice acceptor site	probably null
R5088:Dpy19l4	UTSW	4	11,303,357 (GRCm39)	missense	probably damaging	1.00
R5288:Dpy19l4	UTSW	4	11,304,014 (GRCm39)	missense	probably damaging	1.00
R5288:Dpy19l4	UTSW	4	11,289,721 (GRCm39)	missense	probably damaging	1.00
R5413:Dpy19l4	UTSW	4	11,289,700 (GRCm39)	missense	probably damaging	1.00
R5758:Dpy19l4	UTSW	4	11,276,886 (GRCm39)	missense	probably damaging	1.00
R6024:Dpy19l4	UTSW	4	11,276,876 (GRCm39)	missense	probably damaging	1.00
R6312:Dpy19l4	UTSW	4	11,289,671 (GRCm39)	nonsense	probably null
R6339:Dpy19l4	UTSW	4	11,285,111 (GRCm39)	missense	probably damaging	0.98
R7055:Dpy19l4	UTSW	4	11,290,291 (GRCm39)	critical splice acceptor site	probably null
R7359:Dpy19l4	UTSW	4	11,273,125 (GRCm39)	missense	probably benign	0.00
R7525:Dpy19l4	UTSW	4	11,317,160 (GRCm39)	nonsense	probably null
R7579:Dpy19l4	UTSW	4	11,265,909 (GRCm39)	missense	probably benign	0.39
R7913:Dpy19l4	UTSW	4	11,265,859 (GRCm39)	nonsense	probably null
R8047:Dpy19l4	UTSW	4	11,317,139 (GRCm39)	missense	probably benign	0.00
R8049:Dpy19l4	UTSW	4	11,303,982 (GRCm39)	missense	probably benign	0.44
R8495:Dpy19l4	UTSW	4	11,267,659 (GRCm39)	missense	probably benign
R8911:Dpy19l4	UTSW	4	11,317,078 (GRCm39)	missense	possibly damaging	0.82
R8928:Dpy19l4	UTSW	4	11,304,674 (GRCm39)	intron	probably benign
R8955:Dpy19l4	UTSW	4	11,290,195 (GRCm39)	missense	probably benign	0.00
R9332:Dpy19l4	UTSW	4	11,304,298 (GRCm39)	critical splice donor site	probably null
R9372:Dpy19l4	UTSW	4	11,303,343 (GRCm39)	missense	possibly damaging	0.91
R9401:Dpy19l4	UTSW	4	11,265,806 (GRCm39)	missense	probably benign	0.29

Predicted Primers

PCR Primer
(F):5'- CACATTGCTGCTGTGTGGAAG -3'
(R):5'- TGTCAGCATGCTTCAAGGTG -3'

Sequencing Primer
(F):5'- GAAAGGGTCTCCAACTGCC -3'
(R):5'- CTTCAAGGTGGTCTTTTGGAATAC -3'

Posted On

2014-10-30