Mutagenetix > Incidental Mutation

Incidental Mutation 'R2366:Brd10'

246358

Institutional Source

Beutler Lab

Gene Symbol

Brd10

Ensembl Gene

ENSMUSG00000046138

Gene Name

bromodomain containing 10

Synonyms

9930021J03Rik, Gm9832

MMRRC Submission

040347-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.137) question?

Stock #

R2366 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

29691802-29783389 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 29731035 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 726 (I726N)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000177155]

AlphaFold

H3BKP8

Predicted Effect

probably damaging
Transcript: ENSMUST00000059484
AA Change: I726N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000054060
Gene: ENSMUSG00000046138
AA Change: I726N

Domain	Start	End	E-Value	Type
low complexity region	4	66	N/A	INTRINSIC
BROMO	75	198	1.22e-3	SMART
low complexity region	225	235	N/A	INTRINSIC
coiled coil region	248	289	N/A	INTRINSIC
low complexity region	347	365	N/A	INTRINSIC
low complexity region	495	508	N/A	INTRINSIC
coiled coil region	673	705	N/A	INTRINSIC
low complexity region	722	756	N/A	INTRINSIC
coiled coil region	764	796	N/A	INTRINSIC
low complexity region	1146	1160	N/A	INTRINSIC
internal_repeat_1	1164	1293	9.57e-8	PROSPERO
low complexity region	1295	1304	N/A	INTRINSIC
low complexity region	1386	1415	N/A	INTRINSIC
low complexity region	1468	1483	N/A	INTRINSIC
low complexity region	1602	1624	N/A	INTRINSIC
low complexity region	1647	1663	N/A	INTRINSIC
low complexity region	1732	1745	N/A	INTRINSIC
internal_repeat_1	1766	1910	9.57e-8	PROSPERO
low complexity region	1987	1993	N/A	INTRINSIC
low complexity region	2013	2027	N/A	INTRINSIC
low complexity region	2053	2071	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000175726
AA Change: I603N

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

Predicted Effect

unknown
Transcript: ENSMUST00000176773
AA Change: I9N

Predicted Effect

possibly damaging
Transcript: ENSMUST00000177155
AA Change: I659N

PolyPhen 2 Score 0.734 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000135473
Gene: ENSMUSG00000046138
AA Change: I659N

Domain	Start	End	E-Value	Type
low complexity region	4	66	N/A	INTRINSIC
BROMO	75	198	1.22e-3	SMART
low complexity region	205	219	N/A	INTRINSIC
low complexity region	280	298	N/A	INTRINSIC
low complexity region	428	441	N/A	INTRINSIC
coiled coil region	606	638	N/A	INTRINSIC
low complexity region	655	689	N/A	INTRINSIC
coiled coil region	697	729	N/A	INTRINSIC
low complexity region	1079	1093	N/A	INTRINSIC
internal_repeat_1	1097	1226	1.32e-7	PROSPERO
low complexity region	1228	1237	N/A	INTRINSIC
low complexity region	1319	1348	N/A	INTRINSIC
low complexity region	1401	1416	N/A	INTRINSIC
low complexity region	1535	1557	N/A	INTRINSIC
low complexity region	1580	1596	N/A	INTRINSIC
low complexity region	1665	1678	N/A	INTRINSIC
internal_repeat_1	1699	1843	1.32e-7	PROSPERO
low complexity region	1920	1926	N/A	INTRINSIC
low complexity region	1946	1960	N/A	INTRINSIC
low complexity region	1986	2004	N/A	INTRINSIC

Meta Mutation Damage Score

0.0849

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630091E08Rik	G	A	7: 98,192,949 (GRCm39)		noncoding transcript	Het
Adamts5	C	T	16: 85,659,646 (GRCm39)	G882D	probably damaging	Het
Arl6ip6	T	C	2: 53,082,379 (GRCm39)	V82A	probably benign	Het
Cd38	T	G	5: 44,060,932 (GRCm39)		probably benign	Het
Cep250	G	A	2: 155,834,552 (GRCm39)	R2159K	probably damaging	Het
Col6a6	C	A	9: 105,632,893 (GRCm39)	G1457V	probably damaging	Het
Cyp2c69	T	C	19: 39,866,038 (GRCm39)	N185S	probably benign	Het
Cyp2d12	T	A	15: 82,439,355 (GRCm39)	L3Q	probably damaging	Het
Dnajc28	G	A	16: 91,413,755 (GRCm39)	T187M	probably damaging	Het
Drc1	A	G	5: 30,523,894 (GRCm39)	*754W	probably null	Het
Erbin	G	A	13: 103,981,417 (GRCm39)	H503Y	probably damaging	Het
F3	G	A	3: 121,526,194 (GRCm39)		probably null	Het
Gm10033	A	T	8: 69,826,232 (GRCm39)	M112K	unknown	Het
Gps1	A	C	11: 120,678,945 (GRCm39)	I404L	probably damaging	Het
Hc	T	C	2: 34,903,648 (GRCm39)	N1002S	probably benign	Het
Impg2	T	C	16: 56,080,236 (GRCm39)	I571T	probably benign	Het
Knop1	C	A	7: 118,451,751 (GRCm39)	V323F	possibly damaging	Het
Kntc1	C	T	5: 123,919,255 (GRCm39)	L845F	probably damaging	Het
Lsm5	T	C	6: 56,680,003 (GRCm39)	D53G	probably damaging	Het
Lzts1	T	C	8: 69,593,257 (GRCm39)		probably null	Het
Matr3	A	T	18: 35,721,448 (GRCm39)	N473I	probably damaging	Het
Med1	A	G	11: 98,052,008 (GRCm39)	V452A	probably damaging	Het
Napsa	T	A	7: 44,231,909 (GRCm39)	D44E	probably damaging	Het
Nbeal1	T	C	1: 60,290,511 (GRCm39)	F1036S	probably damaging	Het
Ncapg2	G	A	12: 116,384,349 (GRCm39)	W270*	probably null	Het
Nherf1	G	A	11: 115,054,454 (GRCm39)	V35M	probably benign	Het
Or5af1	C	A	11: 58,722,039 (GRCm39)	Q20K	probably benign	Het
Pik3ca	G	A	3: 32,516,943 (GRCm39)	W1057*	probably null	Het
Pkd1l2	A	T	8: 117,770,056 (GRCm39)	D1133E	probably benign	Het
Pramel39-ps	T	C	5: 94,450,972 (GRCm39)	K385E	probably benign	Het
Prox1	T	A	1: 189,894,079 (GRCm39)	E122V	probably damaging	Het
Rest	C	A	5: 77,416,034 (GRCm39)	H83N	probably benign	Het
Rundc3a	A	G	11: 102,288,491 (GRCm39)	I68V	probably damaging	Het
Stx6	A	T	1: 155,077,706 (GRCm39)	I238L	probably benign	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Ubqln3	C	A	7: 103,790,256 (GRCm39)	Q611H	probably damaging	Het
Usp6nl	T	A	2: 6,445,770 (GRCm39)	H559Q	probably benign	Het
Vipr1	T	G	9: 121,494,250 (GRCm39)	V277G	probably benign	Het
Zfp101	A	T	17: 33,599,972 (GRCm39)	C595S	probably benign	Het
Zfp398	C	T	6: 47,840,143 (GRCm39)	T124I	possibly damaging	Het

Other mutations in Brd10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01311:Brd10	APN	19	29,731,420 (GRCm39)	missense	probably benign	0.33
IGL01535:Brd10	APN	19	29,731,212 (GRCm39)	missense	possibly damaging	0.53
IGL02019:Brd10	APN	19	29,694,463 (GRCm39)	missense	probably benign	0.28
IGL02034:Brd10	APN	19	29,694,259 (GRCm39)	missense	possibly damaging	0.73
IGL03114:Brd10	APN	19	29,694,532 (GRCm39)	missense	probably benign	0.18
IGL03382:Brd10	APN	19	29,694,676 (GRCm39)	missense	probably damaging	1.00
R0020:Brd10	UTSW	19	29,693,597 (GRCm39)	missense	probably damaging	0.98
R0020:Brd10	UTSW	19	29,693,597 (GRCm39)	missense	probably damaging	0.98
R0142:Brd10	UTSW	19	29,695,654 (GRCm39)	missense	possibly damaging	0.93
R0178:Brd10	UTSW	19	29,732,188 (GRCm39)	missense	probably damaging	1.00
R0453:Brd10	UTSW	19	29,731,068 (GRCm39)	missense	probably damaging	1.00
R0730:Brd10	UTSW	19	29,695,381 (GRCm39)	missense	probably benign	0.00
R0735:Brd10	UTSW	19	29,695,038 (GRCm39)	missense	possibly damaging	0.92
R0891:Brd10	UTSW	19	29,695,053 (GRCm39)	missense	probably damaging	1.00
R0894:Brd10	UTSW	19	29,697,974 (GRCm39)	splice site	probably benign
R1289:Brd10	UTSW	19	29,700,852 (GRCm39)	missense	probably benign	0.07
R1368:Brd10	UTSW	19	29,693,796 (GRCm39)	missense	probably damaging	0.97
R1387:Brd10	UTSW	19	29,700,853 (GRCm39)	missense	probably benign	0.15
R1483:Brd10	UTSW	19	29,696,745 (GRCm39)	missense	possibly damaging	0.93
R1526:Brd10	UTSW	19	29,712,545 (GRCm39)	missense	probably damaging	1.00
R1612:Brd10	UTSW	19	29,695,245 (GRCm39)	missense	possibly damaging	0.86
R1721:Brd10	UTSW	19	29,720,998 (GRCm39)	missense	probably damaging	0.99
R1764:Brd10	UTSW	19	29,696,560 (GRCm39)	missense	possibly damaging	0.53
R1822:Brd10	UTSW	19	29,693,814 (GRCm39)	missense	probably damaging	0.99
R1824:Brd10	UTSW	19	29,693,814 (GRCm39)	missense	probably damaging	0.99
R1859:Brd10	UTSW	19	29,732,323 (GRCm39)	missense	possibly damaging	0.53
R1868:Brd10	UTSW	19	29,720,998 (GRCm39)	missense	probably damaging	0.99
R1880:Brd10	UTSW	19	29,695,523 (GRCm39)	missense	probably benign	0.06
R1898:Brd10	UTSW	19	29,712,532 (GRCm39)	missense	possibly damaging	0.74
R1936:Brd10	UTSW	19	29,731,077 (GRCm39)	missense	possibly damaging	0.87
R1939:Brd10	UTSW	19	29,731,077 (GRCm39)	missense	possibly damaging	0.87
R1969:Brd10	UTSW	19	29,694,075 (GRCm39)	missense	possibly damaging	0.95
R2153:Brd10	UTSW	19	29,694,229 (GRCm39)	missense	probably benign	0.01
R2379:Brd10	UTSW	19	29,696,275 (GRCm39)	missense	probably benign	0.01
R3107:Brd10	UTSW	19	29,700,847 (GRCm39)	missense	probably damaging	1.00
R4012:Brd10	UTSW	19	29,720,990 (GRCm39)	missense	probably damaging	1.00
R4222:Brd10	UTSW	19	29,696,149 (GRCm39)	missense	probably benign	0.18
R4328:Brd10	UTSW	19	29,720,961 (GRCm39)	missense	probably benign	0.00
R4329:Brd10	UTSW	19	29,720,961 (GRCm39)	missense	probably benign	0.00
R4387:Brd10	UTSW	19	29,782,715 (GRCm39)	unclassified	probably benign
R4688:Brd10	UTSW	19	29,694,501 (GRCm39)	missense	probably benign	0.33
R4796:Brd10	UTSW	19	29,731,018 (GRCm39)	missense	probably benign	0.33
R4820:Brd10	UTSW	19	29,695,809 (GRCm39)	missense	possibly damaging	0.53
R4832:Brd10	UTSW	19	29,694,616 (GRCm39)	missense	possibly damaging	0.53
R5056:Brd10	UTSW	19	29,694,759 (GRCm39)	missense	probably benign
R5150:Brd10	UTSW	19	29,782,950 (GRCm39)	missense	probably damaging	0.96
R5224:Brd10	UTSW	19	29,696,450 (GRCm39)	missense	possibly damaging	0.73
R5306:Brd10	UTSW	19	29,707,230 (GRCm39)	intron	probably benign
R5460:Brd10	UTSW	19	29,732,250 (GRCm39)	missense	probably damaging	0.98
R5477:Brd10	UTSW	19	29,731,518 (GRCm39)	missense	probably benign	0.33
R5531:Brd10	UTSW	19	29,731,072 (GRCm39)	missense	possibly damaging	0.73
R5559:Brd10	UTSW	19	29,694,363 (GRCm39)	missense	possibly damaging	0.91
R5647:Brd10	UTSW	19	29,731,210 (GRCm39)	missense	possibly damaging	0.73
R5886:Brd10	UTSW	19	29,696,677 (GRCm39)	missense	probably benign	0.03
R6029:Brd10	UTSW	19	29,732,367 (GRCm39)	unclassified	probably benign
R6240:Brd10	UTSW	19	29,694,640 (GRCm39)	missense	probably benign	0.18
R6331:Brd10	UTSW	19	29,695,147 (GRCm39)	missense	probably benign	0.33
R6456:Brd10	UTSW	19	29,693,914 (GRCm39)	missense	possibly damaging	0.93
R6584:Brd10	UTSW	19	29,696,128 (GRCm39)	missense	possibly damaging	0.53
R6661:Brd10	UTSW	19	29,700,864 (GRCm39)	missense	possibly damaging	0.53
R6991:Brd10	UTSW	19	29,696,508 (GRCm39)	missense	possibly damaging	0.86
R7059:Brd10	UTSW	19	29,696,945 (GRCm39)	missense	probably benign	0.33
R7128:Brd10	UTSW	19	29,693,881 (GRCm39)	missense	possibly damaging	0.53
R7211:Brd10	UTSW	19	29,763,712 (GRCm39)	missense
R7471:Brd10	UTSW	19	29,707,139 (GRCm39)	splice site	probably null
R7686:Brd10	UTSW	19	29,694,870 (GRCm39)	missense	probably benign	0.34
R8012:Brd10	UTSW	19	29,695,534 (GRCm39)	missense	possibly damaging	0.73
R8203:Brd10	UTSW	19	29,693,443 (GRCm39)	missense	probably benign	0.18
R8353:Brd10	UTSW	19	29,731,242 (GRCm39)	missense	possibly damaging	0.53
R8672:Brd10	UTSW	19	29,731,564 (GRCm39)	missense	probably benign
R8755:Brd10	UTSW	19	29,693,890 (GRCm39)	missense	probably benign	0.03
R8918:Brd10	UTSW	19	29,696,841 (GRCm39)	missense	possibly damaging	0.53
R8954:Brd10	UTSW	19	29,696,126 (GRCm39)	missense	possibly damaging	0.72
R9038:Brd10	UTSW	19	29,731,900 (GRCm39)	missense	possibly damaging	0.86
R9195:Brd10	UTSW	19	29,763,703 (GRCm39)	missense
R9204:Brd10	UTSW	19	29,696,938 (GRCm39)	missense	possibly damaging	0.73
R9518:Brd10	UTSW	19	29,731,541 (GRCm39)	missense	possibly damaging	0.53
R9743:Brd10	UTSW	19	29,694,261 (GRCm39)	missense	probably benign
R9747:Brd10	UTSW	19	29,731,911 (GRCm39)	missense	possibly damaging	0.73
RF011:Brd10	UTSW	19	29,721,009 (GRCm39)	missense	possibly damaging	0.53
X0027:Brd10	UTSW	19	29,712,599 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GAGCACCTATACTTGTTTTGTCCTTAG -3'
(R):5'- ACTGCCACAAAGTTGTAAGGG -3'

Sequencing Primer
(F):5'- CACCTGTTCTGGGTTTTTAAACATG -3'
(R):5'- GGGATATATTGTTAGAGCACTCACTG -3'

Posted On

2014-10-30