Mutagenetix > Incidental Mutation

Incidental Mutation 'R2406:Mapk15'

248027

Institutional Source

Beutler Lab

Gene Symbol

Mapk15

Ensembl Gene

ENSMUSG00000063704

Gene Name

mitogen-activated protein kinase 15

Synonyms

MMRRC Submission

040372-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2406 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

75865618-75871003 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 75870697 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 512 (S512P)

Ref Sequence

ENSEMBL: ENSMUSP00000087098 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060807] [ENSMUST00000089669] [ENSMUST00000170153]

AlphaFold

Q80Y86

Predicted Effect

probably benign
Transcript: ENSMUST00000060807

SMART Domains

Protein: ENSMUSP00000059839
Gene: ENSMUSG00000046761

Domain	Start	End	E-Value	Type
Pfam:DUF1669	12	283	3.4e-105	PFAM
low complexity region	304	317	N/A	INTRINSIC
low complexity region	562	578	N/A	INTRINSIC
low complexity region	629	652	N/A	INTRINSIC
low complexity region	671	679	N/A	INTRINSIC
low complexity region	685	697	N/A	INTRINSIC
low complexity region	736	747	N/A	INTRINSIC
internal_repeat_1	836	877	9.35e-9	PROSPERO
internal_repeat_1	877	921	9.35e-9	PROSPERO

Predicted Effect

possibly damaging
Transcript: ENSMUST00000089669
AA Change: S512P

PolyPhen 2 Score 0.753 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000087098
Gene: ENSMUSG00000063704
AA Change: S512P

Domain	Start	End	E-Value	Type
S_TKc	14	305	7.08e-97	SMART
low complexity region	391	404	N/A	INTRINSIC
low complexity region	424	434	N/A	INTRINSIC
low complexity region	475	505	N/A	INTRINSIC
low complexity region	513	525	N/A	INTRINSIC
low complexity region	538	548	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160092

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160637

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161961

Predicted Effect

probably benign
Transcript: ENSMUST00000170153

SMART Domains

Protein: ENSMUSP00000126453
Gene: ENSMUSG00000046761

Domain	Start	End	E-Value	Type
Pfam:DUF1669	4	284	2.1e-110	PFAM
low complexity region	304	317	N/A	INTRINSIC
low complexity region	562	578	N/A	INTRINSIC
low complexity region	629	652	N/A	INTRINSIC
low complexity region	671	679	N/A	INTRINSIC
low complexity region	685	697	N/A	INTRINSIC
low complexity region	736	747	N/A	INTRINSIC
internal_repeat_1	836	877	9.35e-9	PROSPERO
internal_repeat_1	877	921	9.35e-9	PROSPERO

Predicted Effect

unknown
Transcript: ENSMUST00000230929
AA Change: S107P

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.9%
20x: 93.9%

Validation Efficiency

95% (42/44)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2300002M23Rik	T	A	17: 35,879,352 (GRCm39)	V230E	probably damaging	Het
Adam22	A	T	5: 8,230,064 (GRCm39)		probably benign	Het
AW554918	A	G	18: 25,473,344 (GRCm39)	I180V	possibly damaging	Het
C9	T	C	15: 6,512,780 (GRCm39)	S301P	possibly damaging	Het
Cdc42bpb	T	C	12: 111,268,558 (GRCm39)	Q1214R	probably benign	Het
Chaf1b	T	C	16: 93,697,043 (GRCm39)	I351T	probably damaging	Het
Clip1	T	C	5: 123,741,723 (GRCm39)	E1177G	probably damaging	Het
Colgalt1	G	A	8: 72,070,312 (GRCm39)	C198Y	probably damaging	Het
Dennd4b	C	T	3: 90,182,795 (GRCm39)	R871C	probably damaging	Het
Efcab3	A	T	11: 104,611,457 (GRCm39)	D433V	probably benign	Het
Eif4ebp1	A	G	8: 27,763,362 (GRCm39)	I52V	probably damaging	Het
Fbxw5	T	C	2: 25,394,195 (GRCm39)	I86T	probably damaging	Het
Gm10650	A	G	3: 127,833,530 (GRCm39)		noncoding transcript	Het
Helz	G	A	11: 107,577,378 (GRCm39)	A1910T	unknown	Het
Hsf2	C	T	10: 57,373,642 (GRCm39)	T70I	probably damaging	Het
Ifih1	A	G	2: 62,437,447 (GRCm39)		probably benign	Het
Kdm2a	T	C	19: 4,372,546 (GRCm39)	D933G	probably damaging	Het
Macc1	T	C	12: 119,429,346 (GRCm39)	I832T	probably damaging	Het
Mybpc2	A	T	7: 44,171,149 (GRCm39)	I134N	possibly damaging	Het
Myh4	A	G	11: 67,150,000 (GRCm39)	E1853G	probably damaging	Het
Ncoa3	T	C	2: 165,897,279 (GRCm39)	V690A	probably damaging	Het
Nsf	C	T	11: 103,821,578 (GRCm39)	E26K	possibly damaging	Het
Or5b97	A	G	19: 12,878,991 (GRCm39)	V51A	probably benign	Het
Pacsin2	A	T	15: 83,269,313 (GRCm39)		probably benign	Het
Piezo2	A	T	18: 63,155,596 (GRCm39)	L2416Q	probably damaging	Het
Pip5k1c	T	A	10: 81,144,858 (GRCm39)	I233N	probably damaging	Het
Ptcd3	T	C	6: 71,865,631 (GRCm39)	D428G	probably damaging	Het
Ptprf	T	C	4: 118,126,501 (GRCm39)	D84G	possibly damaging	Het
Rasgrf2	T	C	13: 92,120,359 (GRCm39)	D798G	probably benign	Het
Setd7	A	G	3: 51,450,097 (GRCm39)	Y110H	probably damaging	Het
Sgta	T	C	10: 80,887,081 (GRCm39)	I61M	possibly damaging	Het
Shank1	A	G	7: 44,006,376 (GRCm39)	D2031G	possibly damaging	Het
Skint5	A	G	4: 113,799,864 (GRCm39)	Y88H	probably damaging	Het
Spata31f1a	A	T	4: 42,851,696 (GRCm39)	D153E	probably benign	Het
Stox1	T	C	10: 62,499,945 (GRCm39)	I872V	probably benign	Het
Tmc4	A	G	7: 3,674,025 (GRCm39)	F385L	probably benign	Het
Top3a	A	G	11: 60,646,838 (GRCm39)	F258L	probably damaging	Het
Ttn	T	C	2: 76,780,499 (GRCm39)	Y1084C	probably damaging	Het
Ubtf	A	T	11: 102,199,528 (GRCm39)	Y504*	probably null	Het
Wdfy3	T	C	5: 102,036,125 (GRCm39)	T2098A	probably damaging	Het
Wnk2	G	T	13: 49,214,964 (GRCm39)	T1194K	possibly damaging	Het
Zbtb40	T	A	4: 136,725,879 (GRCm39)	E560V	probably benign	Het

Other mutations in Mapk15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01595:Mapk15	APN	15	75,867,129 (GRCm39)	missense	probably benign	0.04
IGL02075:Mapk15	APN	15	75,866,737 (GRCm39)	missense	probably benign	0.35
IGL02395:Mapk15	APN	15	75,870,019 (GRCm39)	missense	probably benign
IGL03052:Mapk15	UTSW	15	75,865,731 (GRCm39)	missense	probably benign	0.01
R0008:Mapk15	UTSW	15	75,870,103 (GRCm39)	missense	probably benign	0.08
R0109:Mapk15	UTSW	15	75,867,926 (GRCm39)	nonsense	probably null
R0109:Mapk15	UTSW	15	75,867,926 (GRCm39)	nonsense	probably null
R1148:Mapk15	UTSW	15	75,870,004 (GRCm39)	missense	probably benign
R1148:Mapk15	UTSW	15	75,870,004 (GRCm39)	missense	probably benign
R4526:Mapk15	UTSW	15	75,867,104 (GRCm39)	missense	possibly damaging	0.83
R4572:Mapk15	UTSW	15	75,870,599 (GRCm39)	splice site	probably benign
R4613:Mapk15	UTSW	15	75,867,759 (GRCm39)	missense	probably damaging	0.98
R5861:Mapk15	UTSW	15	75,868,208 (GRCm39)	unclassified	probably benign
R6912:Mapk15	UTSW	15	75,865,747 (GRCm39)	missense	probably damaging	0.99
R7554:Mapk15	UTSW	15	75,867,745 (GRCm39)	missense	possibly damaging	0.63
R7620:Mapk15	UTSW	15	75,870,697 (GRCm39)	missense	probably benign	0.00
R7923:Mapk15	UTSW	15	75,868,295 (GRCm39)	missense	probably damaging	1.00
R9308:Mapk15	UTSW	15	75,865,714 (GRCm39)	nonsense	probably null
R9744:Mapk15	UTSW	15	75,869,912 (GRCm39)	missense	possibly damaging	0.78
Z1177:Mapk15	UTSW	15	75,870,310 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TTCCAGGTGAAGACTCAAGTG -3'
(R):5'- AGAAGATGCATTGGGGTCTG -3'

Sequencing Primer
(F):5'- AGGCTCTGATCCGCAGTGATC -3'
(R):5'- CATTGGGGTCTGGGGGAC -3'

Posted On

2014-11-11