Mutagenetix > Incidental Mutation

Incidental Mutation 'R2442:Vmn2r82'

249827

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r82

Ensembl Gene

ENSMUSG00000091468

Gene Name

vomeronasal 2, receptor 82

Synonyms

EG624845

MMRRC Submission

040400-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R2442 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

79192425-79232600 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 79221210 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 524 (S524G)

Ref Sequence

ENSEMBL: ENSMUSP00000130114 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170596]

AlphaFold

G3UWA2

Predicted Effect

probably damaging
Transcript: ENSMUST00000170596
AA Change: S524G

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000130114
Gene: ENSMUSG00000091468
AA Change: S524G

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	6e-35	PFAM
Pfam:NCD3G	517	570	9.3e-22	PFAM
Pfam:7tm_3	603	838	6.5e-49	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	G	17: 24,547,606 (GRCm39)	V256A	probably benign	Het
Acap1	T	C	11: 69,780,317 (GRCm39)	N42S	possibly damaging	Het
Apoo-ps	C	A	13: 107,551,140 (GRCm39)		noncoding transcript	Het
Bri3	C	T	5: 144,181,411 (GRCm39)	T39I	probably benign	Het
Bsn	A	G	9: 107,984,119 (GRCm39)	S3312P	unknown	Het
Camkmt	C	A	17: 85,398,203 (GRCm39)	A17E	possibly damaging	Het
Cc2d2a	G	T	5: 43,828,647 (GRCm39)		probably null	Het
Ccdc122	G	T	14: 77,329,398 (GRCm39)	M150I	possibly damaging	Het
Celf4	A	G	18: 25,886,516 (GRCm39)	F57L	probably damaging	Het
Cep192	T	A	18: 67,957,759 (GRCm39)	F564Y	possibly damaging	Het
Ces1c	T	C	8: 93,849,840 (GRCm39)	D38G	probably damaging	Het
Cts7	C	A	13: 61,503,431 (GRCm39)	G178*	probably null	Het
Dcc	A	T	18: 71,589,954 (GRCm39)	Y681N	probably damaging	Het
Dhx29	A	G	13: 113,083,508 (GRCm39)	E521G	possibly damaging	Het
Dnase2a	T	C	8: 85,635,622 (GRCm39)	V35A	probably damaging	Het
Eif3l	A	G	15: 78,969,807 (GRCm39)	M268V	probably damaging	Het
Foxc1	A	G	13: 31,992,781 (GRCm39)	M531V	unknown	Het
Grin2c	A	G	11: 115,141,960 (GRCm39)	Y820H	probably damaging	Het
Ifit1bl1	G	A	19: 34,572,289 (GRCm39)	A56V	probably benign	Het
Iqsec1	T	C	6: 90,666,865 (GRCm39)	E524G	possibly damaging	Het
Kcnt2	A	G	1: 140,304,091 (GRCm39)	I154V	possibly damaging	Het
Kdm1b	T	A	13: 47,216,451 (GRCm39)	Y274N	probably benign	Het
Kntc1	T	A	5: 123,948,922 (GRCm39)	L1889Q	probably damaging	Het
Lama1	A	G	17: 68,075,312 (GRCm39)	T1010A	probably benign	Het
Mmp11	T	C	10: 75,763,079 (GRCm39)	N171S	probably benign	Het
Myom1	A	G	17: 71,417,730 (GRCm39)	E1409G	probably damaging	Het
N4bp1	A	G	8: 87,588,668 (GRCm39)	I90T	probably damaging	Het
Or4c52	T	C	2: 89,845,685 (GRCm39)	V137A	probably benign	Het
Plcb4	T	C	2: 135,792,302 (GRCm39)	S342P	probably damaging	Het
Plg	A	G	17: 12,629,847 (GRCm39)	E627G	probably benign	Het
Rsf1	CG	CGACGGCGGAG	7: 97,229,115 (GRCm39)		probably benign	Het
Sla2	G	A	2: 156,717,862 (GRCm39)	R137C	probably damaging	Het
Slc7a14	A	G	3: 31,284,469 (GRCm39)	I289T	probably damaging	Het
Srpra	G	A	9: 35,123,297 (GRCm39)	G43S	possibly damaging	Het
Tbc1d9	T	C	8: 83,892,705 (GRCm39)	M1T	probably null	Het
Tcte2	A	T	17: 13,934,339 (GRCm39)	I90N	possibly damaging	Het
Trim30c	G	A	7: 104,031,481 (GRCm39)	P445S	probably damaging	Het
Trrap	A	G	5: 144,754,776 (GRCm39)	Q1984R	probably damaging	Het
Ubn2	T	C	6: 38,467,940 (GRCm39)	S885P	probably benign	Het
Unc45a	A	G	7: 79,989,417 (GRCm39)	F17S	probably damaging	Het
Uts2b	C	T	16: 27,179,782 (GRCm39)	V75I	probably benign	Het
Vmn2r103	A	G	17: 19,993,793 (GRCm39)	K57E	probably benign	Het

Other mutations in Vmn2r82

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Vmn2r82	APN	10	79,192,581 (GRCm39)	missense	probably benign	0.03
IGL01860:Vmn2r82	APN	10	79,214,691 (GRCm39)	missense	probably benign	0.18
IGL01927:Vmn2r82	APN	10	79,213,906 (GRCm39)	missense	probably damaging	1.00
IGL01929:Vmn2r82	APN	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
IGL02028:Vmn2r82	APN	10	79,215,057 (GRCm39)	missense	probably benign
IGL02112:Vmn2r82	APN	10	79,231,833 (GRCm39)	missense	probably benign	0.19
IGL02632:Vmn2r82	APN	10	79,192,542 (GRCm39)	missense	probably benign	0.45
IGL02665:Vmn2r82	APN	10	79,215,205 (GRCm39)	missense	probably damaging	0.99
IGL02716:Vmn2r82	APN	10	79,213,678 (GRCm39)	missense	probably benign	0.20
IGL03030:Vmn2r82	APN	10	79,217,149 (GRCm39)	missense	possibly damaging	0.85
IGL03190:Vmn2r82	APN	10	79,192,643 (GRCm39)	splice site	probably null
IGL03349:Vmn2r82	APN	10	79,213,703 (GRCm39)	missense	probably benign	0.25
IGL03048:Vmn2r82	UTSW	10	79,232,460 (GRCm39)	missense	probably damaging	0.98
R0080:Vmn2r82	UTSW	10	79,232,339 (GRCm39)	missense	probably benign	0.00
R0193:Vmn2r82	UTSW	10	79,217,129 (GRCm39)	missense	probably damaging	1.00
R0217:Vmn2r82	UTSW	10	79,214,634 (GRCm39)	missense	possibly damaging	0.46
R0285:Vmn2r82	UTSW	10	79,232,391 (GRCm39)	missense	probably damaging	1.00
R1193:Vmn2r82	UTSW	10	79,213,739 (GRCm39)	nonsense	probably null
R1385:Vmn2r82	UTSW	10	79,232,325 (GRCm39)	nonsense	probably null
R1386:Vmn2r82	UTSW	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
R1442:Vmn2r82	UTSW	10	79,215,201 (GRCm39)	missense	probably benign	0.03
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1518:Vmn2r82	UTSW	10	79,214,702 (GRCm39)	missense	probably damaging	1.00
R1538:Vmn2r82	UTSW	10	79,192,578 (GRCm39)	missense	possibly damaging	0.92
R1607:Vmn2r82	UTSW	10	79,215,253 (GRCm39)	missense	possibly damaging	0.67
R1812:Vmn2r82	UTSW	10	79,215,046 (GRCm39)	missense	probably benign	0.33
R1906:Vmn2r82	UTSW	10	79,232,344 (GRCm39)	missense	probably damaging	1.00
R1954:Vmn2r82	UTSW	10	79,231,890 (GRCm39)	missense	probably damaging	1.00
R1972:Vmn2r82	UTSW	10	79,214,680 (GRCm39)	missense	probably damaging	1.00
R2093:Vmn2r82	UTSW	10	79,231,813 (GRCm39)	missense	probably benign	0.30
R2156:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R2202:Vmn2r82	UTSW	10	79,192,519 (GRCm39)	missense	probably benign
R2444:Vmn2r82	UTSW	10	79,213,702 (GRCm39)	missense	possibly damaging	0.65
R2857:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2858:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2884:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R2886:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R4369:Vmn2r82	UTSW	10	79,231,914 (GRCm39)	missense	probably benign	0.01
R4445:Vmn2r82	UTSW	10	79,214,874 (GRCm39)	missense	possibly damaging	0.87
R4589:Vmn2r82	UTSW	10	79,192,548 (GRCm39)	missense	probably damaging	1.00
R4703:Vmn2r82	UTSW	10	79,214,641 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r82	UTSW	10	79,214,589 (GRCm39)	missense	probably benign	0.00
R4937:Vmn2r82	UTSW	10	79,215,010 (GRCm39)	missense	probably benign	0.01
R5199:Vmn2r82	UTSW	10	79,231,921 (GRCm39)	missense	probably damaging	1.00
R5391:Vmn2r82	UTSW	10	79,192,491 (GRCm39)	missense	probably null	0.01
R5601:Vmn2r82	UTSW	10	79,232,025 (GRCm39)	missense	probably damaging	1.00
R5635:Vmn2r82	UTSW	10	79,214,652 (GRCm39)	missense	probably benign	0.33
R6065:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R6074:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R6340:Vmn2r82	UTSW	10	79,231,727 (GRCm39)	missense	probably benign	0.00
R6474:Vmn2r82	UTSW	10	79,214,871 (GRCm39)	missense	possibly damaging	0.55
R6995:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R7111:Vmn2r82	UTSW	10	79,214,605 (GRCm39)	missense	probably benign	0.22
R7212:Vmn2r82	UTSW	10	79,215,268 (GRCm39)	missense	probably benign	0.00
R7335:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R7353:Vmn2r82	UTSW	10	79,232,452 (GRCm39)	missense	probably benign	0.11
R7354:Vmn2r82	UTSW	10	79,192,464 (GRCm39)	missense	probably benign	0.00
R7362:Vmn2r82	UTSW	10	79,232,451 (GRCm39)	missense	probably benign	0.00
R7378:Vmn2r82	UTSW	10	79,232,276 (GRCm39)	nonsense	probably null
R7430:Vmn2r82	UTSW	10	79,217,087 (GRCm39)	missense	probably damaging	1.00
R7509:Vmn2r82	UTSW	10	79,231,842 (GRCm39)	missense	possibly damaging	0.82
R7874:Vmn2r82	UTSW	10	79,232,345 (GRCm39)	missense	probably damaging	1.00
R7943:Vmn2r82	UTSW	10	79,232,079 (GRCm39)	missense	possibly damaging	0.74
R8158:Vmn2r82	UTSW	10	79,213,636 (GRCm39)	missense	probably benign	0.12
R8324:Vmn2r82	UTSW	10	79,214,727 (GRCm39)	nonsense	probably null
R8340:Vmn2r82	UTSW	10	79,217,036 (GRCm39)	missense	probably benign	0.00
R8787:Vmn2r82	UTSW	10	79,213,894 (GRCm39)	missense	probably damaging	1.00
R8929:Vmn2r82	UTSW	10	79,232,541 (GRCm39)	missense	probably benign	0.00
R9018:Vmn2r82	UTSW	10	79,232,539 (GRCm39)	missense	probably damaging	1.00
R9399:Vmn2r82	UTSW	10	79,214,768 (GRCm39)	nonsense	probably null
R9517:Vmn2r82	UTSW	10	79,213,641 (GRCm39)	nonsense	probably null
R9587:Vmn2r82	UTSW	10	79,214,936 (GRCm39)	missense	possibly damaging	0.70
R9602:Vmn2r82	UTSW	10	79,214,880 (GRCm39)	missense	probably benign	0.07
Z1088:Vmn2r82	UTSW	10	79,192,456 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,232,369 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,192,429 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- ATGGCCGTTGCTGACCAAAG -3'
(R):5'- ATGGCCATGTGTTTCCCTTG -3'

Sequencing Primer
(F):5'- CCACTTTCATTCAATGGCCAATGG -3'
(R):5'- TCCTCTGAGAAACTTGTCC -3'

Posted On

2014-11-12