Incidental Mutation 'R2412:Zfp85'
| ID |
250042 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp85
|
| Ensembl Gene |
ENSMUSG00000058331 |
| Gene Name |
zinc finger protein 85 |
| Synonyms |
Zfp85-rs1, KRAB19, Zfp71 |
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.079)
|
| Stock # |
R2412 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
13 |
| Chromosomal Location |
67895919-67903347 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 67897765 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Methionine
at position 102
(I102M)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000121202
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000091520]
[ENSMUST00000144183]
|
| AlphaFold |
Q7TMC9 |
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000049518
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000091520
|
| SMART Domains |
Protein: ENSMUSP00000089105
Gene: ENSMUSG00000058331
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
5.78e-29 |
SMART |
|
ZnF_C2H2
|
81 |
103 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
109 |
131 |
1.22e-4 |
SMART |
|
ZnF_C2H2
|
137 |
159 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
165 |
187 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
193 |
215 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
221 |
243 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
249 |
271 |
1.1e-2 |
SMART |
|
ZnF_C2H2
|
277 |
299 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
305 |
327 |
2.49e-1 |
SMART |
|
ZnF_C2H2
|
333 |
355 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
361 |
383 |
4.17e-3 |
SMART |
|
ZnF_C2H2
|
389 |
411 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
417 |
439 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
445 |
467 |
1.04e-3 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000143812
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000144183
AA Change: I102M
PolyPhen 2
Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000121202
Gene: ENSMUSG00000058331
AA Change: I102M
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
45 |
105 |
5.78e-29 |
SMART |
|
ZnF_C2H2
|
121 |
143 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
149 |
171 |
1.22e-4 |
SMART |
|
ZnF_C2H2
|
177 |
199 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
205 |
227 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
233 |
255 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
261 |
283 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
289 |
311 |
1.1e-2 |
SMART |
|
ZnF_C2H2
|
317 |
339 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
345 |
367 |
2.49e-1 |
SMART |
|
ZnF_C2H2
|
373 |
395 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
401 |
423 |
4.17e-3 |
SMART |
|
ZnF_C2H2
|
429 |
451 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
457 |
479 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
485 |
507 |
1.04e-3 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000181158
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.5%
- 10x: 97.1%
- 20x: 94.6%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 21 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acad9 |
A |
T |
3: 36,127,740 (GRCm39) |
Q114L |
probably benign |
Het |
| Adamts3 |
A |
G |
5: 89,849,630 (GRCm39) |
Y597H |
probably damaging |
Het |
| Bmi1 |
A |
G |
2: 18,688,525 (GRCm39) |
H172R |
probably damaging |
Het |
| Cyp3a44 |
A |
T |
5: 145,716,389 (GRCm39) |
Y400* |
probably null |
Het |
| Dennd5b |
T |
C |
6: 148,906,736 (GRCm39) |
T1062A |
possibly damaging |
Het |
| Dync2h1 |
T |
C |
9: 7,144,246 (GRCm39) |
D1065G |
probably benign |
Het |
| Ephb1 |
G |
A |
9: 101,879,015 (GRCm39) |
R492C |
possibly damaging |
Het |
| Fat4 |
A |
T |
3: 39,011,221 (GRCm39) |
H2107L |
probably benign |
Het |
| Itga8 |
A |
G |
2: 12,306,526 (GRCm39) |
V23A |
probably benign |
Het |
| Lrba |
A |
G |
3: 86,235,007 (GRCm39) |
N851D |
probably damaging |
Het |
| Ltbp3 |
A |
G |
19: 5,796,673 (GRCm39) |
T322A |
probably benign |
Het |
| Ntpcr |
T |
C |
8: 126,472,144 (GRCm39) |
V151A |
probably damaging |
Het |
| Or8h7 |
T |
C |
2: 86,721,178 (GRCm39) |
I114V |
probably benign |
Het |
| Or9g3 |
C |
A |
2: 85,590,024 (GRCm39) |
G232V |
probably damaging |
Het |
| Pramel51 |
A |
G |
12: 88,143,880 (GRCm39) |
V311A |
probably damaging |
Het |
| Rps6ka4 |
A |
T |
19: 6,807,309 (GRCm39) |
*774K |
probably null |
Het |
| Slc12a5 |
T |
C |
2: 164,818,382 (GRCm39) |
|
probably null |
Het |
| Tmem219 |
T |
C |
7: 126,495,939 (GRCm39) |
S37G |
probably damaging |
Het |
| Ttn |
A |
T |
2: 76,641,587 (GRCm39) |
L5176Q |
possibly damaging |
Het |
| Tubb1 |
A |
G |
2: 174,298,903 (GRCm39) |
N195S |
possibly damaging |
Het |
| Zan |
A |
G |
5: 137,412,425 (GRCm39) |
|
probably null |
Het |
|
| Other mutations in Zfp85 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
R0383:Zfp85
|
UTSW |
13 |
67,896,791 (GRCm39) |
missense |
probably benign |
0.05 |
|
R1187:Zfp85
|
UTSW |
13 |
67,897,835 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1510:Zfp85
|
UTSW |
13 |
67,903,084 (GRCm39) |
intron |
probably benign |
|
|
R1775:Zfp85
|
UTSW |
13 |
67,897,823 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1803:Zfp85
|
UTSW |
13 |
67,899,747 (GRCm39) |
missense |
probably benign |
0.15 |
|
R2108:Zfp85
|
UTSW |
13 |
67,897,003 (GRCm39) |
missense |
probably benign |
0.08 |
|
R3147:Zfp85
|
UTSW |
13 |
67,900,612 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R4811:Zfp85
|
UTSW |
13 |
67,897,745 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5308:Zfp85
|
UTSW |
13 |
67,896,974 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5334:Zfp85
|
UTSW |
13 |
67,899,803 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5373:Zfp85
|
UTSW |
13 |
67,897,577 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7045:Zfp85
|
UTSW |
13 |
67,897,712 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7391:Zfp85
|
UTSW |
13 |
67,897,410 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7438:Zfp85
|
UTSW |
13 |
67,897,064 (GRCm39) |
missense |
probably benign |
0.09 |
|
R7544:Zfp85
|
UTSW |
13 |
67,897,184 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8046:Zfp85
|
UTSW |
13 |
67,897,098 (GRCm39) |
nonsense |
probably null |
|
|
R8112:Zfp85
|
UTSW |
13 |
67,896,893 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R9533:Zfp85
|
UTSW |
13 |
67,897,722 (GRCm39) |
missense |
probably benign |
0.08 |
|
R9615:Zfp85
|
UTSW |
13 |
67,897,326 (GRCm39) |
missense |
probably damaging |
0.98 |
|
| Predicted Primers |
PCR Primer
(F):5'- ATTTGTAGCGTTTCTCTCCAGT -3'
(R):5'- GTGGCTCACAACCATCCATAA -3'
Sequencing Primer
(F):5'- CAGTGTGGATTCTTTGATGCTTACCC -3'
(R):5'- CCCCATAAATGTAAAGAGTGTG -3'
|
| Posted On |
2014-11-12 |
Incidental Mutation