Mutagenetix > Incidental Mutation

Incidental Mutation 'R0309:Akap9'

25046

Institutional Source

Beutler Lab

Gene Symbol

Akap9

Ensembl Gene

ENSMUSG00000040407

Gene Name

A kinase anchor protein 9

Synonyms

mei2-5, repro12, AKAP450, G1-448-15, 5730481H23Rik

MMRRC Submission

038519-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.351) question?

Stock #

R0309 (G1)

Quality Score

191

Status

Validated

Chromosome

Chromosomal Location

3977410-4130204 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 4119038 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 3515 (D3515G)

Ref Sequence

ENSEMBL: ENSMUSP00000046129 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044492] [ENSMUST00000176863]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000044492
AA Change: D3515G

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000046129
Gene: ENSMUSG00000040407
AA Change: D3515G

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	98	115	N/A	INTRINSIC
Blast:HPT	126	197	6e-21	BLAST
low complexity region	237	249	N/A	INTRINSIC
low complexity region	297	315	N/A	INTRINSIC
coiled coil region	404	593	N/A	INTRINSIC
coiled coil region	622	756	N/A	INTRINSIC
coiled coil region	777	843	N/A	INTRINSIC
coiled coil region	888	958	N/A	INTRINSIC
low complexity region	982	997	N/A	INTRINSIC
coiled coil region	1037	1065	N/A	INTRINSIC
low complexity region	1233	1246	N/A	INTRINSIC
internal_repeat_2	1247	1312	7.75e-5	PROSPERO
internal_repeat_1	1377	1485	2.63e-5	PROSPERO
coiled coil region	1522	1589	N/A	INTRINSIC
coiled coil region	1789	2107	N/A	INTRINSIC
coiled coil region	2132	2318	N/A	INTRINSIC
internal_repeat_1	2322	2445	2.63e-5	PROSPERO
coiled coil region	2455	2494	N/A	INTRINSIC
low complexity region	2587	2598	N/A	INTRINSIC
low complexity region	2627	2640	N/A	INTRINSIC
internal_repeat_2	2934	2997	7.75e-5	PROSPERO
low complexity region	3000	3016	N/A	INTRINSIC
coiled coil region	3109	3307	N/A	INTRINSIC
coiled coil region	3455	3493	N/A	INTRINSIC
coiled coil region	3521	3556	N/A	INTRINSIC
Pfam:PACT_coil_coil	3576	3657	1.2e-27	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000147629

Predicted Effect

probably benign
Transcript: ENSMUST00000176863

SMART Domains

Protein: ENSMUSP00000135836
Gene: ENSMUSG00000040407

Domain	Start	End	E-Value	Type
coiled coil region	1	90	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196837

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197366

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000198500

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 98.7%
3x: 97.6%
10x: 94.3%
20x: 86.4%

Validation Efficiency

98% (125/127)

MGI Phenotype

PHENOTYPE: Mice homozygous for a chemically induced allele exhibit male infertily with abnormal spermatogenesis and Sertoli maturation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 126 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402F06Rik	T	C	2: 35,266,271 (GRCm39)	D133G	possibly damaging	Het
Abcb4	A	C	5: 8,989,835 (GRCm39)	D796A	probably damaging	Het
Actg2	A	T	6: 83,496,896 (GRCm39)	V147E	probably damaging	Het
Adamts13	A	C	2: 26,877,001 (GRCm39)	T534P	probably damaging	Het
Ago1	T	C	4: 126,336,959 (GRCm39)	T249A	probably benign	Het
Ahnak	T	A	19: 8,979,859 (GRCm39)	I381N	probably damaging	Het
Angptl3	T	C	4: 98,922,706 (GRCm39)	V249A	probably benign	Het
Ank	A	G	15: 27,567,658 (GRCm39)	T294A	possibly damaging	Het
Ank1	A	T	8: 23,594,825 (GRCm39)	H204L	probably damaging	Het
Apbb2	A	G	5: 66,468,331 (GRCm39)		probably benign	Het
Arhgap28	A	T	17: 68,208,424 (GRCm39)	S15T	probably benign	Het
Aspm	T	C	1: 139,410,249 (GRCm39)		probably benign	Het
Atp1a4	T	C	1: 172,062,554 (GRCm39)	E651G	probably damaging	Het
B3gnt2	A	T	11: 22,786,860 (GRCm39)	F109L	probably damaging	Het
Bpifb4	T	C	2: 153,801,603 (GRCm39)	F575L	probably damaging	Het
Calhm4	A	G	10: 33,920,043 (GRCm39)	W75R	probably damaging	Het
Calr	C	A	8: 85,569,660 (GRCm39)	K322N	probably benign	Het
Ccdc188	T	C	16: 18,037,169 (GRCm39)	S247P	possibly damaging	Het
Cdr1	T	A	X: 60,228,908 (GRCm39)	D86V	unknown	Het
Cep97	C	T	16: 55,745,421 (GRCm39)	V48I	probably damaging	Het
Chaf1b	T	A	16: 93,681,399 (GRCm39)	C6S	probably damaging	Het
Chd3	C	T	11: 69,247,844 (GRCm39)	D920N	probably damaging	Het
Clk1	T	C	1: 58,452,192 (GRCm39)		probably benign	Het
Cntnap3	T	A	13: 64,905,250 (GRCm39)		probably benign	Het
Col12a1	T	A	9: 79,507,293 (GRCm39)		probably null	Het
Col17a1	G	T	19: 47,659,801 (GRCm39)		probably benign	Het
Coq7	T	A	7: 118,128,940 (GRCm39)	I32F	possibly damaging	Het
Cox6a2	A	T	7: 127,805,107 (GRCm39)	F59I	probably damaging	Het
Cpq	A	G	15: 33,594,297 (GRCm39)	D436G	probably damaging	Het
Ctso	G	A	3: 81,852,168 (GRCm39)		probably null	Het
Cxadr	A	T	16: 78,131,836 (GRCm39)	H274L	probably benign	Het
Cyp2c40	A	T	19: 39,766,495 (GRCm39)	C367S	possibly damaging	Het
Cyp2c70	T	G	19: 40,149,115 (GRCm39)	M344L	possibly damaging	Het
Defa35	G	A	8: 21,555,871 (GRCm39)	V77I	probably benign	Het
Dhx57	A	G	17: 80,582,310 (GRCm39)	Y432H	probably damaging	Het
Dhx9	A	T	1: 153,341,441 (GRCm39)	D601E	probably benign	Het
Dnah7a	C	G	1: 53,444,849 (GRCm39)	D3952H	probably damaging	Het
Dnah9	C	A	11: 65,917,798 (GRCm39)		probably benign	Het
Dstyk	C	A	1: 132,384,602 (GRCm39)		probably benign	Het
Efcab2	T	A	1: 178,303,469 (GRCm39)		probably benign	Het
Ehbp1l1	T	C	19: 5,770,598 (GRCm39)	E287G	possibly damaging	Het
Epgn	A	G	5: 91,180,073 (GRCm39)	T87A	probably benign	Het
Erc2	A	C	14: 27,863,182 (GRCm39)	E803A	probably damaging	Het
Fer	A	G	17: 64,446,011 (GRCm39)	*454W	probably null	Het
Glyr1	T	C	16: 4,849,836 (GRCm39)	D179G	probably damaging	Het
Gm12830	T	A	4: 114,702,173 (GRCm39)		probably benign	Het
Gm9922	C	A	14: 101,967,129 (GRCm39)		probably benign	Het
Gsta3	C	T	1: 21,335,118 (GRCm39)	P200S	possibly damaging	Het
Hmgxb3	G	A	18: 61,288,200 (GRCm39)		probably benign	Het
Hsh2d	G	A	8: 72,954,304 (GRCm39)	D229N	probably benign	Het
Il16	T	C	7: 83,371,762 (GRCm39)	K15E	probably damaging	Het
Kcnip2	T	A	19: 45,782,514 (GRCm39)		probably benign	Het
Kdm4c	T	C	4: 74,263,804 (GRCm39)	V696A	probably benign	Het
Kdr	A	G	5: 76,107,587 (GRCm39)		probably benign	Het
Klhl33	T	G	14: 51,128,868 (GRCm39)	H787P	probably damaging	Het
Klk14	A	T	7: 43,343,769 (GRCm39)	T159S	probably benign	Het
Lancl2	A	G	6: 57,680,117 (GRCm39)	N16D	probably damaging	Het
Lemd3	T	C	10: 120,773,015 (GRCm39)	N583S	possibly damaging	Het
Map3k4	TGCTGGCTTCAGGGCCACAGTCCGCTG	TGCTG	17: 12,489,902 (GRCm39)		probably null	Het
Mpl	T	G	4: 118,303,235 (GRCm39)		probably benign	Het
Myh7b	T	C	2: 155,472,592 (GRCm39)		probably benign	Het
Mylk	A	C	16: 34,732,667 (GRCm39)		probably benign	Het
Myof	A	T	19: 37,969,714 (GRCm39)	M316K	probably benign	Het
Nfib	T	A	4: 82,214,974 (GRCm39)	N543I	probably damaging	Het
Nfix	A	G	8: 85,448,403 (GRCm39)	S375P	probably damaging	Het
Nkrf	T	C	X: 36,153,769 (GRCm39)	Q171R	probably damaging	Het
Nmnat2	T	A	1: 152,952,747 (GRCm39)		probably benign	Het
Npffr2	G	A	5: 89,731,206 (GRCm39)	E379K	probably benign	Het
Npr2	T	C	4: 43,640,904 (GRCm39)		probably benign	Het
Nup98	A	C	7: 101,801,635 (GRCm39)	D212E	probably null	Het
Nwd2	T	C	5: 63,964,561 (GRCm39)	Y1382H	probably damaging	Het
Ocstamp	T	C	2: 165,237,912 (GRCm39)	R451G	possibly damaging	Het
Or52s1	T	A	7: 102,861,928 (GRCm39)	I287K	probably damaging	Het
Or6c6c	A	G	10: 129,541,008 (GRCm39)	D87G	probably benign	Het
Pabpc1	C	T	15: 36,597,737 (GRCm39)	A551T	possibly damaging	Het
Pard3	A	T	8: 128,103,378 (GRCm39)		probably benign	Het
Pcdhb12	G	T	18: 37,569,174 (GRCm39)	V107L	probably benign	Het
Pik3cd	A	T	4: 149,747,677 (GRCm39)	V22D	probably damaging	Het
Pkd1l2	A	G	8: 117,724,315 (GRCm39)	V2396A	probably damaging	Het
Pnpla7	T	C	2: 24,877,207 (GRCm39)	I167T	probably damaging	Het
Pphln1	A	T	15: 93,339,588 (GRCm39)	H114L	possibly damaging	Het
Ppm1h	A	G	10: 122,756,687 (GRCm39)	N444S	probably damaging	Het
Prdm9	G	A	17: 15,777,646 (GRCm39)	T146I	probably damaging	Het
Prrc2a	A	G	17: 35,369,891 (GRCm39)		probably benign	Het
Prrx1	T	C	1: 163,140,128 (GRCm39)	D26G	possibly damaging	Het
Ptpn5	T	C	7: 46,729,042 (GRCm39)	E495G	probably damaging	Het
Rab23	A	C	1: 33,773,942 (GRCm39)		probably null	Het
Ralgps1	C	T	2: 33,047,935 (GRCm39)	M348I	probably benign	Het
Ranbp2	A	G	10: 58,315,690 (GRCm39)	T2137A	probably benign	Het
Rapgef4	G	T	2: 72,056,374 (GRCm39)	G654V	probably benign	Het
Rc3h2	A	T	2: 37,269,020 (GRCm39)		probably benign	Het
Reg2	G	A	6: 78,383,169 (GRCm39)	A39T	possibly damaging	Het
Sema4d	C	A	13: 51,879,347 (GRCm39)	V7F	probably benign	Het
Sgip1	T	C	4: 102,772,354 (GRCm39)		probably benign	Het
Sgpl1	C	T	10: 60,949,216 (GRCm39)		probably null	Het
Shisa9	G	A	16: 11,814,987 (GRCm39)	V212M	probably damaging	Het
Shq1	G	A	6: 100,550,588 (GRCm39)	P450L	probably benign	Het
Sin3a	A	G	9: 57,018,196 (GRCm39)	T872A	probably benign	Het
Sipa1l3	C	T	7: 29,047,775 (GRCm39)	R1371Q	probably benign	Het
Skint8	T	C	4: 111,796,064 (GRCm39)	V246A	probably benign	Het
Slc22a20	A	T	19: 6,022,985 (GRCm39)	V386D	probably damaging	Het
Slc28a2b	A	T	2: 122,348,034 (GRCm39)	T253S	probably benign	Het
Slc2a7	G	A	4: 150,242,528 (GRCm39)		probably benign	Het
Slc35a2	T	A	X: 7,755,901 (GRCm39)	Y48N	probably damaging	Het
Slc4a2	G	T	5: 24,639,344 (GRCm39)	S413I	probably damaging	Het
Sntg2	T	C	12: 30,276,772 (GRCm39)	T427A	probably benign	Het
Soat1	T	C	1: 156,270,023 (GRCm39)	Y132C	probably damaging	Het
Stn1	G	T	19: 47,490,112 (GRCm39)	H342N	probably benign	Het
Tarbp1	T	A	8: 127,165,667 (GRCm39)		probably benign	Het
Tas2r113	A	C	6: 132,870,341 (GRCm39)	K123T	probably damaging	Het
Tbck	C	T	3: 132,440,168 (GRCm39)	Q504*	probably null	Het
Tenm3	C	T	8: 48,794,069 (GRCm39)	C380Y	probably damaging	Het
Tent4a	A	T	13: 69,648,051 (GRCm39)	V781E	possibly damaging	Het
Triobp	A	G	15: 78,860,740 (GRCm39)	D1389G	probably damaging	Het
Trpm4	A	T	7: 44,958,130 (GRCm39)	F780I	probably damaging	Het
Tubb4a	G	T	17: 57,388,182 (GRCm39)	Y281*	probably null	Het
Txndc15	T	C	13: 55,872,395 (GRCm39)	F261S	probably damaging	Het
Ube3b	T	C	5: 114,557,530 (GRCm39)		probably benign	Het
Unc5c	G	C	3: 141,439,694 (GRCm39)	V196L	probably benign	Het
Upf3a	G	A	8: 13,845,500 (GRCm39)		probably null	Het
Vmn2r20	T	C	6: 123,363,063 (GRCm39)	K574E	probably benign	Het
Vps50	A	G	6: 3,536,853 (GRCm39)	M275V	possibly damaging	Het
Xrcc5	A	G	1: 72,346,735 (GRCm39)		probably benign	Het
Zbtb18	T	C	1: 177,276,182 (GRCm39)	L505S	probably damaging	Het
Zbtb41	T	C	1: 139,366,722 (GRCm39)	I567T	probably damaging	Het
Zfp598	T	C	17: 24,897,558 (GRCm39)		probably benign	Het

Other mutations in Akap9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00478:Akap9	APN	5	4,096,639 (GRCm39)	missense	probably damaging	0.97
IGL00642:Akap9	APN	5	4,010,842 (GRCm39)	missense	probably damaging	0.99
IGL00786:Akap9	APN	5	4,120,522 (GRCm39)	missense	probably damaging	1.00
IGL00788:Akap9	APN	5	4,110,480 (GRCm39)	missense	probably damaging	1.00
IGL00969:Akap9	APN	5	4,051,550 (GRCm39)	missense	probably benign
IGL01014:Akap9	APN	5	4,018,683 (GRCm39)	missense	probably benign	0.41
IGL01302:Akap9	APN	5	4,020,711 (GRCm39)	missense	probably benign	0.27
IGL01610:Akap9	APN	5	4,082,839 (GRCm39)	missense	possibly damaging	0.95
IGL01620:Akap9	APN	5	4,010,218 (GRCm39)	missense	probably benign	0.11
IGL01862:Akap9	APN	5	4,115,856 (GRCm39)	missense	probably damaging	0.99
IGL01862:Akap9	APN	5	4,001,705 (GRCm39)	missense	probably damaging	0.99
IGL02151:Akap9	APN	5	4,082,728 (GRCm39)	nonsense	probably null
IGL02635:Akap9	APN	5	4,120,500 (GRCm39)	missense	possibly damaging	0.59
IGL02858:Akap9	APN	5	4,119,130 (GRCm39)	missense	possibly damaging	0.88
IGL02967:Akap9	APN	5	4,026,164 (GRCm39)	missense	probably benign	0.07
IGL03064:Akap9	APN	5	4,018,755 (GRCm39)	missense	probably damaging	1.00
IGL03289:Akap9	APN	5	4,127,261 (GRCm39)	missense	probably damaging	1.00
Andy	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
blimey	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
hoarder	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
marinarum	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
miser	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
naviculus	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
thrifty	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
wee_one	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
FR4449:Akap9	UTSW	5	4,031,214 (GRCm39)	unclassified	probably benign
PIT1430001:Akap9	UTSW	5	4,079,849 (GRCm39)	missense	probably damaging	1.00
PIT4366001:Akap9	UTSW	5	4,096,221 (GRCm39)	missense	probably benign	0.24
R0088:Akap9	UTSW	5	4,011,946 (GRCm39)	missense	probably benign	0.22
R0387:Akap9	UTSW	5	4,001,678 (GRCm39)	splice site	probably benign
R0440:Akap9	UTSW	5	4,114,569 (GRCm39)	missense	probably damaging	0.99
R0441:Akap9	UTSW	5	4,011,714 (GRCm39)	missense	probably benign	0.15
R0491:Akap9	UTSW	5	4,022,851 (GRCm39)	unclassified	probably benign
R0501:Akap9	UTSW	5	4,020,685 (GRCm39)	missense	probably damaging	1.00
R0507:Akap9	UTSW	5	4,119,043 (GRCm39)	missense	probably benign	0.41
R0544:Akap9	UTSW	5	4,119,185 (GRCm39)	missense	probably benign	0.22
R0581:Akap9	UTSW	5	4,100,620 (GRCm39)	missense	probably benign	0.03
R0611:Akap9	UTSW	5	4,004,870 (GRCm39)	missense	probably benign	0.00
R0620:Akap9	UTSW	5	4,114,136 (GRCm39)	missense	probably damaging	0.98
R0639:Akap9	UTSW	5	4,110,318 (GRCm39)	missense	probably damaging	1.00
R0932:Akap9	UTSW	5	4,096,492 (GRCm39)	missense	possibly damaging	0.77
R0944:Akap9	UTSW	5	4,114,742 (GRCm39)	splice site	probably null
R1101:Akap9	UTSW	5	4,096,205 (GRCm39)	missense	probably benign	0.00
R1159:Akap9	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
R1170:Akap9	UTSW	5	4,105,671 (GRCm39)	missense	probably benign
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1453:Akap9	UTSW	5	4,025,614 (GRCm39)	splice site	probably null
R1551:Akap9	UTSW	5	4,119,174 (GRCm39)	missense	probably benign	0.02
R1608:Akap9	UTSW	5	4,011,783 (GRCm39)	missense	probably damaging	1.00
R1652:Akap9	UTSW	5	4,127,210 (GRCm39)	missense	probably damaging	1.00
R1659:Akap9	UTSW	5	4,114,633 (GRCm39)	missense	probably damaging	1.00
R1713:Akap9	UTSW	5	4,089,345 (GRCm39)	critical splice donor site	probably null
R1719:Akap9	UTSW	5	4,007,645 (GRCm39)	nonsense	probably null
R1720:Akap9	UTSW	5	4,022,791 (GRCm39)	missense	possibly damaging	0.63
R1757:Akap9	UTSW	5	4,051,667 (GRCm39)	missense	probably benign	0.41
R1872:Akap9	UTSW	5	4,051,406 (GRCm39)	missense	probably damaging	1.00
R1876:Akap9	UTSW	5	4,011,809 (GRCm39)	missense	probably benign	0.28
R1881:Akap9	UTSW	5	4,100,173 (GRCm39)	missense	probably benign
R1950:Akap9	UTSW	5	4,010,677 (GRCm39)	missense	probably damaging	1.00
R1980:Akap9	UTSW	5	4,022,771 (GRCm39)	missense	probably damaging	0.99
R1993:Akap9	UTSW	5	4,088,520 (GRCm39)	splice site	probably null
R2008:Akap9	UTSW	5	4,010,131 (GRCm39)	missense	possibly damaging	0.47
R2020:Akap9	UTSW	5	4,011,967 (GRCm39)	missense	probably damaging	1.00
R2051:Akap9	UTSW	5	4,025,685 (GRCm39)	nonsense	probably null
R2061:Akap9	UTSW	5	4,011,010 (GRCm39)	missense	probably damaging	1.00
R2109:Akap9	UTSW	5	4,094,847 (GRCm39)	missense	possibly damaging	0.47
R2135:Akap9	UTSW	5	4,114,509 (GRCm39)	missense	probably damaging	1.00
R2225:Akap9	UTSW	5	4,127,271 (GRCm39)	missense	probably damaging	0.96
R2232:Akap9	UTSW	5	4,096,603 (GRCm39)	missense	probably damaging	1.00
R2424:Akap9	UTSW	5	4,115,279 (GRCm39)	missense	probably damaging	0.97
R2483:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R2879:Akap9	UTSW	5	4,026,353 (GRCm39)	intron	probably benign
R3622:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3623:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3624:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3722:Akap9	UTSW	5	4,120,351 (GRCm39)	missense	probably damaging	1.00
R3806:Akap9	UTSW	5	4,004,410 (GRCm39)	missense	probably benign	0.00
R3919:Akap9	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
R4023:Akap9	UTSW	5	4,042,077 (GRCm39)	missense	possibly damaging	0.66
R4093:Akap9	UTSW	5	4,093,996 (GRCm39)	missense	probably damaging	0.99
R4434:Akap9	UTSW	5	4,082,708 (GRCm39)	missense	probably damaging	0.99
R4529:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4530:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4532:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4533:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4585:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4586:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4655:Akap9	UTSW	5	4,096,403 (GRCm39)	missense	probably benign	0.14
R4676:Akap9	UTSW	5	4,114,515 (GRCm39)	nonsense	probably null
R4676:Akap9	UTSW	5	4,082,774 (GRCm39)	missense	probably damaging	1.00
R4724:Akap9	UTSW	5	4,105,339 (GRCm39)	missense	probably benign
R4731:Akap9	UTSW	5	4,012,266 (GRCm39)	missense	possibly damaging	0.54
R4732:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4733:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4743:Akap9	UTSW	5	4,011,013 (GRCm39)	missense	probably damaging	1.00
R4749:Akap9	UTSW	5	4,018,737 (GRCm39)	missense	probably benign	0.41
R4756:Akap9	UTSW	5	4,051,418 (GRCm39)	missense	probably damaging	0.99
R4757:Akap9	UTSW	5	4,058,382 (GRCm39)	missense	probably damaging	1.00
R4860:Akap9	UTSW	5	4,084,916 (GRCm39)	intron	probably benign
R4937:Akap9	UTSW	5	4,100,145 (GRCm39)	splice site	probably null
R4960:Akap9	UTSW	5	4,007,664 (GRCm39)	missense	probably benign	0.15
R4974:Akap9	UTSW	5	4,011,466 (GRCm39)	missense	possibly damaging	0.81
R5101:Akap9	UTSW	5	4,051,748 (GRCm39)	missense	probably damaging	0.96
R5160:Akap9	UTSW	5	4,080,007 (GRCm39)	missense	probably damaging	1.00
R5200:Akap9	UTSW	5	4,010,734 (GRCm39)	missense	probably benign	0.00
R5245:Akap9	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
R5293:Akap9	UTSW	5	3,998,687 (GRCm39)	missense	probably damaging	0.99
R5408:Akap9	UTSW	5	4,108,458 (GRCm39)	missense	possibly damaging	0.84
R5507:Akap9	UTSW	5	4,018,683 (GRCm39)	missense	probably benign	0.41
R5517:Akap9	UTSW	5	4,051,665 (GRCm39)	missense	possibly damaging	0.76
R5579:Akap9	UTSW	5	4,114,714 (GRCm39)	missense	possibly damaging	0.93
R5619:Akap9	UTSW	5	4,004,760 (GRCm39)	intron	probably benign
R5645:Akap9	UTSW	5	4,100,590 (GRCm39)	missense	probably benign	0.09
R5669:Akap9	UTSW	5	4,100,540 (GRCm39)	nonsense	probably null
R5686:Akap9	UTSW	5	4,021,926 (GRCm39)	missense	probably benign	0.00
R5697:Akap9	UTSW	5	4,010,170 (GRCm39)	missense	possibly damaging	0.92
R5821:Akap9	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
R5875:Akap9	UTSW	5	4,127,285 (GRCm39)	missense	probably benign	0.01
R5897:Akap9	UTSW	5	4,127,904 (GRCm39)	missense	probably benign	0.23
R5999:Akap9	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
R6025:Akap9	UTSW	5	4,082,801 (GRCm39)	missense	probably damaging	1.00
R6078:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6138:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6225:Akap9	UTSW	5	4,012,105 (GRCm39)	missense	probably damaging	1.00
R6243:Akap9	UTSW	5	4,115,000 (GRCm39)	splice site	probably null
R6326:Akap9	UTSW	5	4,012,061 (GRCm39)	missense	probably damaging	1.00
R6564:Akap9	UTSW	5	4,078,491 (GRCm39)	missense	probably damaging	0.98
R6617:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6625:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6632:Akap9	UTSW	5	4,063,842 (GRCm39)	splice site	probably null
R6677:Akap9	UTSW	5	4,079,869 (GRCm39)	missense	probably benign	0.21
R6717:Akap9	UTSW	5	4,114,086 (GRCm39)	missense	probably damaging	1.00
R6893:Akap9	UTSW	5	4,011,709 (GRCm39)	missense	probably benign	0.32
R6915:Akap9	UTSW	5	4,010,551 (GRCm39)	missense	probably benign	0.03
R6938:Akap9	UTSW	5	4,096,628 (GRCm39)	missense	possibly damaging	0.91
R6972:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6973:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6993:Akap9	UTSW	5	4,115,866 (GRCm39)	missense	possibly damaging	0.65
R7032:Akap9	UTSW	5	4,004,896 (GRCm39)	missense	probably benign
R7164:Akap9	UTSW	5	4,110,364 (GRCm39)	missense	probably damaging	0.96
R7170:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7192:Akap9	UTSW	5	4,055,723 (GRCm39)	splice site	probably null
R7284:Akap9	UTSW	5	4,006,246 (GRCm39)	missense	probably damaging	1.00
R7299:Akap9	UTSW	5	4,082,696 (GRCm39)	missense	probably damaging	1.00
R7313:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7326:Akap9	UTSW	5	4,095,930 (GRCm39)	missense	possibly damaging	0.47
R7343:Akap9	UTSW	5	4,096,364 (GRCm39)	missense	probably damaging	0.99
R7455:Akap9	UTSW	5	4,022,792 (GRCm39)	missense	probably benign	0.03
R7482:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7489:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7525:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7528:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7576:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7577:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7578:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7610:Akap9	UTSW	5	4,007,677 (GRCm39)	missense	possibly damaging	0.95
R7658:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7754:Akap9	UTSW	5	4,096,736 (GRCm39)	missense	probably benign	0.03
R7818:Akap9	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
R7979:Akap9	UTSW	5	4,100,381 (GRCm39)	missense	probably benign
R7991:Akap9	UTSW	5	4,114,949 (GRCm39)	splice site	probably null
R8036:Akap9	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
R8054:Akap9	UTSW	5	4,088,707 (GRCm39)	critical splice donor site	probably null
R8116:Akap9	UTSW	5	4,111,183 (GRCm39)	missense	probably benign	0.04
R8150:Akap9	UTSW	5	4,011,982 (GRCm39)	missense	probably damaging	1.00
R8234:Akap9	UTSW	5	4,094,845 (GRCm39)	missense	probably benign	0.18
R8348:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8365:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8366:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8448:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8466:Akap9	UTSW	5	4,088,659 (GRCm39)	missense	probably damaging	1.00
R8772:Akap9	UTSW	5	4,096,255 (GRCm39)	missense	probably damaging	1.00
R8881:Akap9	UTSW	5	4,011,279 (GRCm39)	missense
R8937:Akap9	UTSW	5	4,094,048 (GRCm39)	missense	possibly damaging	0.78
R8956:Akap9	UTSW	5	3,998,805 (GRCm39)	missense	possibly damaging	0.79
R9000:Akap9	UTSW	5	4,105,650 (GRCm39)	missense	probably benign
R9049:Akap9	UTSW	5	4,114,597 (GRCm39)	missense
R9074:Akap9	UTSW	5	4,127,959 (GRCm39)	missense	probably benign	0.40
R9124:Akap9	UTSW	5	4,111,284 (GRCm39)	missense	probably damaging	0.99
R9129:Akap9	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
R9371:Akap9	UTSW	5	4,011,852 (GRCm39)	missense	possibly damaging	0.83
R9424:Akap9	UTSW	5	4,012,224 (GRCm39)	nonsense	probably null
R9424:Akap9	UTSW	5	4,012,223 (GRCm39)	nonsense	probably null
R9509:Akap9	UTSW	5	4,096,349 (GRCm39)	missense	probably benign
R9515:Akap9	UTSW	5	4,105,709 (GRCm39)	missense	probably damaging	1.00
R9567:Akap9	UTSW	5	4,127,311 (GRCm39)	missense	possibly damaging	0.89
R9587:Akap9	UTSW	5	4,119,149 (GRCm39)	missense	probably damaging	1.00
R9619:Akap9	UTSW	5	4,094,833 (GRCm39)	missense	probably damaging	1.00
R9635:Akap9	UTSW	5	4,100,545 (GRCm39)	missense	probably benign	0.20
R9680:Akap9	UTSW	5	4,011,587 (GRCm39)	missense	probably benign	0.03
R9691:Akap9	UTSW	5	4,010,491 (GRCm39)	missense	probably damaging	1.00
R9726:Akap9	UTSW	5	4,053,757 (GRCm39)	missense	probably benign	0.39
U15987:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
X0026:Akap9	UTSW	5	4,064,039 (GRCm39)	missense	probably damaging	1.00
X0057:Akap9	UTSW	5	4,025,598 (GRCm39)	critical splice acceptor site	probably null
Z1176:Akap9	UTSW	5	4,012,251 (GRCm39)	missense	probably damaging	0.96
Z1177:Akap9	UTSW	5	4,096,189 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAACACCTAGACCCAGACAGTGTG -3'
(R):5'- CAACTGTGCCAGACTGAAAAGATGC -3'

Sequencing Primer
(F):5'- ACCCAGACAGTGTGATTGTAAC -3'
(R):5'- AGCAAGCACTTAGCGTTCTG -3'

Posted On

2013-04-16