Mutagenetix > Incidental Mutation

Incidental Mutation 'R2847:Zfp985'

251670

Institutional Source

Beutler Lab

Gene Symbol

Zfp985

Ensembl Gene

ENSMUSG00000065999

Gene Name

zinc finger protein 985

Synonyms

Gm13154

MMRRC Submission

040440-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.624) question?

Stock #

R2847 (G1)

Quality Score

164

Status

Not validated

Chromosome

Chromosomal Location

147637734-147669655 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 147667468 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Stop codon at position 112 (W112*)

Ref Sequence

ENSEMBL: ENSMUSP00000123296 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081742] [ENSMUST00000139784] [ENSMUST00000143885]

AlphaFold

A2A7A5

Predicted Effect

probably null
Transcript: ENSMUST00000081742
AA Change: W112*

SMART Domains

Protein: ENSMUSP00000080438
Gene: ENSMUSG00000065999
AA Change: W112*

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART
ZnF_C2H2	238	260	8.34e-3	SMART
ZnF_C2H2	266	288	1.47e-3	SMART
ZnF_C2H2	294	316	2.36e-2	SMART
ZnF_C2H2	322	344	8.34e-3	SMART
ZnF_C2H2	350	372	7.67e-2	SMART
ZnF_C2H2	378	400	8.6e-5	SMART
ZnF_C2H2	406	428	8.6e-5	SMART
ZnF_C2H2	434	456	7.9e-4	SMART
ZnF_C2H2	462	484	1.95e-3	SMART
ZnF_C2H2	490	512	2.09e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000139784
AA Change: W112*

SMART Domains

Protein: ENSMUSP00000123296
Gene: ENSMUSG00000065999
AA Change: W112*

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000143885

SMART Domains

Protein: ENSMUSP00000121177
Gene: ENSMUSG00000065999

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930510E17Rik	T	C	9: 53,176,089 (GRCm39)		noncoding transcript	Het
Abca13	T	C	11: 9,244,584 (GRCm39)	V2149A	possibly damaging	Het
Abca8a	T	C	11: 109,932,931 (GRCm39)	D1231G	probably damaging	Het
Adgrf5	C	A	17: 43,733,531 (GRCm39)	N118K	possibly damaging	Het
Atxn1	A	T	13: 45,720,175 (GRCm39)	D573E	probably damaging	Het
Bub3	C	T	7: 131,172,613 (GRCm39)	T326M	possibly damaging	Het
Cd151	T	C	7: 141,049,463 (GRCm39)	Y57H	probably damaging	Het
Cib4	T	C	5: 30,645,932 (GRCm39)	N112S	probably damaging	Het
Cntnap5c	A	T	17: 58,183,387 (GRCm39)	D31V	probably damaging	Het
Cobl	A	G	11: 12,328,342 (GRCm39)	L81P	probably damaging	Het
Cpsf1	A	T	15: 76,487,051 (GRCm39)	L209Q	probably damaging	Het
Crocc	G	A	4: 140,746,067 (GRCm39)	A1684V	probably damaging	Het
Cyp4f37	T	A	17: 32,848,099 (GRCm39)	C206S	probably damaging	Het
Defb39	C	T	8: 19,102,909 (GRCm39)	R62H	possibly damaging	Het
Dennd2b	T	C	7: 109,124,544 (GRCm39)	Q1099R	probably damaging	Het
Dnah6	T	C	6: 73,106,314 (GRCm39)	K1756E	probably benign	Het
Efcab12	A	G	6: 115,788,072 (GRCm39)	I630T	probably damaging	Het
Erc2	T	C	14: 27,762,445 (GRCm39)	V736A	probably damaging	Het
Fbf1	C	T	11: 116,048,514 (GRCm39)		probably null	Het
Fndc9	C	T	11: 46,128,868 (GRCm39)	A129V	probably damaging	Het
Foxk2	CGGGGGG	CGGGGGGGGG	11: 121,151,317 (GRCm39)		probably benign	Het
Gba2	T	C	4: 43,568,000 (GRCm39)		probably null	Het
Gna12	T	A	5: 140,771,348 (GRCm39)	D61V	probably damaging	Het
Gpr37	C	T	6: 25,666,945 (GRCm39)		probably benign	Het
Grin2a	A	G	16: 9,579,829 (GRCm39)	F145L	possibly damaging	Het
Grin2b	C	A	6: 135,717,951 (GRCm39)	V714L	probably damaging	Het
Grm7	G	T	6: 110,623,309 (GRCm39)	V161F	probably damaging	Het
Hmcn1	G	T	1: 150,439,350 (GRCm39)	Y5494*	probably null	Het
Htr4	T	C	18: 62,561,197 (GRCm39)	S153P	probably damaging	Het
Igkv9-120	T	A	6: 68,027,128 (GRCm39)		probably benign	Het
Itgb6	T	C	2: 60,430,879 (GRCm39)	T772A	probably damaging	Het
Mgam	C	A	6: 40,629,649 (GRCm39)	A86E	possibly damaging	Het
Mme	T	A	3: 63,252,620 (GRCm39)	N421K	possibly damaging	Het
Mmp1b	C	T	9: 7,370,763 (GRCm39)	V331I	probably benign	Het
Naa16	A	G	14: 79,573,323 (GRCm39)	C816R	probably damaging	Het
Nav1	G	C	1: 135,378,382 (GRCm39)		probably null	Het
Nln	A	G	13: 104,161,533 (GRCm39)	M679T	probably damaging	Het
Or1n1b	A	G	2: 36,780,536 (GRCm39)	L108P	probably damaging	Het
Or8b53	C	T	9: 38,667,332 (GRCm39)	T116I	possibly damaging	Het
Osbpl8	T	A	10: 111,105,297 (GRCm39)	S251T	probably benign	Het
Otop3	T	C	11: 115,235,384 (GRCm39)	F339L	probably damaging	Het
Pax7	T	C	4: 139,506,954 (GRCm39)	D361G	possibly damaging	Het
Peg10	C	T	6: 4,756,912 (GRCm39)		probably benign	Het
Plekhh2	G	T	17: 84,905,394 (GRCm39)	R1096L	probably damaging	Het
Poteg	A	T	8: 27,971,704 (GRCm39)	N406I	probably benign	Het
Rnf43	T	A	11: 87,623,093 (GRCm39)	N731K	probably benign	Het
Robo4	C	T	9: 37,315,772 (GRCm39)	R342*	probably null	Het
Sec23ip	G	A	7: 128,355,797 (GRCm39)	V307I	probably benign	Het
Slc2a4	T	A	11: 69,836,997 (GRCm39)	N116Y	probably damaging	Het
Tas1r3	T	A	4: 155,944,659 (GRCm39)	Q854L	probably benign	Het
Tox3	G	A	8: 90,975,018 (GRCm39)	Q538*	probably null	Het
Trpm4	A	G	7: 44,960,022 (GRCm39)	F771S	probably damaging	Het
Tstd3	A	T	4: 21,759,375 (GRCm39)	F132L	possibly damaging	Het
Ulk2	T	C	11: 61,715,555 (GRCm39)		probably null	Het
Unc13b	T	C	4: 43,180,404 (GRCm39)	Y3080H	probably benign	Het
Utp25	A	T	1: 192,810,759 (GRCm39)	N81K	probably benign	Het
Vmn1r181	G	T	7: 23,683,943 (GRCm39)	S136I	possibly damaging	Het
Vmn2r114	A	T	17: 23,509,948 (GRCm39)	M844K	probably benign	Het
Vmn2r60	A	T	7: 41,785,857 (GRCm39)	H220L	probably benign	Het
Vps13a	A	G	19: 16,680,963 (GRCm39)	S1078P	probably damaging	Het
Vwa8	G	T	14: 79,184,582 (GRCm39)	R360L	probably benign	Het
Xlr4b	A	T	X: 72,258,938 (GRCm39)	Q25L	probably null	Het
Zdhhc22	T	A	12: 87,035,336 (GRCm39)	T39S	probably benign	Het
Zfp532	T	A	18: 65,789,697 (GRCm39)	H1045Q	possibly damaging	Het
Zfp773	AGCTGCTGCTGCTGCTGCTGCTGCTGC	AGCTGCTGCTGCTGCTGCTGCTGC	7: 7,136,092 (GRCm39)		probably benign	Het
Zfp964	G	C	8: 70,116,504 (GRCm39)	C368S	unknown	Het

Other mutations in Zfp985

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0098:Zfp985	UTSW	4	147,661,566 (GRCm39)	missense	probably damaging	0.97
R0324:Zfp985	UTSW	4	147,667,314 (GRCm39)	missense	probably benign	0.00
R1307:Zfp985	UTSW	4	147,667,704 (GRCm39)	missense	probably benign
R1594:Zfp985	UTSW	4	147,667,537 (GRCm39)	missense	probably benign	0.05
R1657:Zfp985	UTSW	4	147,668,567 (GRCm39)	missense	probably benign	0.01
R1667:Zfp985	UTSW	4	147,668,407 (GRCm39)	missense	possibly damaging	0.84
R1761:Zfp985	UTSW	4	147,668,502 (GRCm39)	missense	probably benign	0.00
R1858:Zfp985	UTSW	4	147,667,315 (GRCm39)	missense	probably benign	0.29
R2509:Zfp985	UTSW	4	147,667,443 (GRCm39)	missense	possibly damaging	0.75
R2510:Zfp985	UTSW	4	147,667,443 (GRCm39)	missense	possibly damaging	0.75
R2848:Zfp985	UTSW	4	147,667,468 (GRCm39)	nonsense	probably null
R4245:Zfp985	UTSW	4	147,667,396 (GRCm39)	missense	probably damaging	0.96
R4260:Zfp985	UTSW	4	147,668,029 (GRCm39)	missense	probably damaging	1.00
R4434:Zfp985	UTSW	4	147,668,368 (GRCm39)	missense	probably benign	0.37
R4480:Zfp985	UTSW	4	147,668,536 (GRCm39)	missense	probably benign	0.07
R4512:Zfp985	UTSW	4	147,668,020 (GRCm39)	missense	probably damaging	1.00
R4514:Zfp985	UTSW	4	147,668,020 (GRCm39)	missense	probably damaging	1.00
R4528:Zfp985	UTSW	4	147,667,347 (GRCm39)	missense	possibly damaging	0.49
R4836:Zfp985	UTSW	4	147,668,612 (GRCm39)	missense	probably damaging	0.97
R4884:Zfp985	UTSW	4	147,667,801 (GRCm39)	missense	probably benign	0.04
R5054:Zfp985	UTSW	4	147,667,438 (GRCm39)	missense	probably damaging	0.98
R5106:Zfp985	UTSW	4	147,668,612 (GRCm39)	missense	probably damaging	0.97
R5205:Zfp985	UTSW	4	147,667,368 (GRCm39)	missense	probably damaging	1.00
R5266:Zfp985	UTSW	4	147,667,289 (GRCm39)	critical splice acceptor site	probably null
R5468:Zfp985	UTSW	4	147,667,702 (GRCm39)	missense	probably benign
R5533:Zfp985	UTSW	4	147,667,440 (GRCm39)	nonsense	probably null
R6282:Zfp985	UTSW	4	147,667,805 (GRCm39)	missense	probably benign	0.00
R6303:Zfp985	UTSW	4	147,668,232 (GRCm39)	missense	probably benign	0.01
R6609:Zfp985	UTSW	4	147,668,124 (GRCm39)	missense	probably damaging	1.00
R6609:Zfp985	UTSW	4	147,667,578 (GRCm39)	missense	probably benign
R6722:Zfp985	UTSW	4	147,667,528 (GRCm39)	missense	probably benign	0.26
R6858:Zfp985	UTSW	4	147,667,764 (GRCm39)	nonsense	probably null
R7064:Zfp985	UTSW	4	147,667,573 (GRCm39)	missense	probably benign	0.20
R7216:Zfp985	UTSW	4	147,667,913 (GRCm39)	missense	probably damaging	1.00
R7471:Zfp985	UTSW	4	147,667,388 (GRCm39)	missense	possibly damaging	0.75
R7583:Zfp985	UTSW	4	147,667,946 (GRCm39)	nonsense	probably null
R7685:Zfp985	UTSW	4	147,667,331 (GRCm39)	missense	probably benign	0.00
R8242:Zfp985	UTSW	4	147,668,639 (GRCm39)	missense	possibly damaging	0.52
R8504:Zfp985	UTSW	4	147,667,883 (GRCm39)	missense	possibly damaging	0.70
R8780:Zfp985	UTSW	4	147,668,412 (GRCm39)	missense	possibly damaging	0.79
R8785:Zfp985	UTSW	4	147,668,080 (GRCm39)	missense	probably damaging	1.00
R9485:Zfp985	UTSW	4	147,668,280 (GRCm39)	missense	probably damaging	1.00
R9513:Zfp985	UTSW	4	147,667,999 (GRCm39)	missense	probably damaging	1.00
R9631:Zfp985	UTSW	4	147,665,742 (GRCm39)	missense	probably damaging	1.00
R9722:Zfp985	UTSW	4	147,667,618 (GRCm39)	missense	possibly damaging	0.63
R9786:Zfp985	UTSW	4	147,668,047 (GRCm39)	missense	probably benign
X0050:Zfp985	UTSW	4	147,667,728 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- AGTAATTCATGTCATCTCTAATTGCC -3'
(R):5'- TGTTTCACCATGTCTTTATGTTTAGA -3'

Sequencing Primer
(F):5'- TCAGAAAATCATTGCATACGTGG -3'
(R):5'- GAAACAAAAACCTTCTCAAATTCTGC -3'

Posted On

2014-12-04