Mutagenetix > Incidental Mutation

Incidental Mutation 'R2656:Or2ag17'

252752

Institutional Source

Beutler Lab

Gene Symbol

Or2ag17

Ensembl Gene

ENSMUSG00000096714

Gene Name

olfactory receptor family 2 subfamily AG member 17

Synonyms

MOR283-10P, GA_x6K02T2PBJ9-9168355-9167405, Olfr699

MMRRC Submission

040431-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.111) question?

Stock #

R2656 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

106389256-106390206 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 106389720 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 163 (T163S)

Ref Sequence

ENSEMBL: ENSMUSP00000149112 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065024] [ENSMUST00000215952] [ENSMUST00000216307]

AlphaFold

Q7TRN3

Predicted Effect

probably damaging
Transcript: ENSMUST00000065024
AA Change: T163S

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000068023
Gene: ENSMUSG00000096714
AA Change: T163S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.3e-46	PFAM
Pfam:7TM_GPCR_Srsx	35	303	2.1e-5	PFAM
Pfam:7tm_1	41	290	1.1e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215952
AA Change: T163S

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000216307
AA Change: T163S

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam22	A	G	5: 8,167,696 (GRCm39)	C655R	probably damaging	Het
Adgrf3	A	T	5: 30,401,436 (GRCm39)	V864D	possibly damaging	Het
Angptl3	A	G	4: 98,926,201 (GRCm39)	I444V	probably benign	Het
Atp8b2	C	T	3: 89,849,065 (GRCm39)	A1090T	probably benign	Het
Bcl2l2	C	T	14: 55,122,889 (GRCm39)	R47C	probably benign	Het
Ces2a	G	A	8: 105,462,766 (GRCm39)	M118I	probably benign	Het
Cyp4a29	T	G	4: 115,106,921 (GRCm39)	L193V	possibly damaging	Het
Dpp8	A	G	9: 64,988,086 (GRCm39)	Y877C	probably damaging	Het
Enpp6	C	T	8: 47,535,453 (GRCm39)	R33*	probably null	Het
Fsip2	G	A	2: 82,809,389 (GRCm39)	D1903N	possibly damaging	Het
Ggt1	T	A	10: 75,417,219 (GRCm39)	Y5*	probably null	Het
Gm14412	A	T	2: 177,006,993 (GRCm39)	C301S	unknown	Het
Gpatch2l	T	A	12: 86,335,584 (GRCm39)	D428E	probably damaging	Het
Grin2b	C	T	6: 135,710,427 (GRCm39)	G1040S	probably damaging	Het
Has2	T	C	15: 56,545,224 (GRCm39)	D126G	possibly damaging	Het
Itgam	T	G	7: 127,715,987 (GRCm39)	L1120R	probably null	Het
Kank4	T	C	4: 98,667,194 (GRCm39)	N418D	probably damaging	Het
Kcnk3	T	C	5: 30,780,015 (GRCm39)	V355A	possibly damaging	Het
Lonrf2	C	T	1: 38,855,041 (GRCm39)		probably null	Het
Lrp1b	A	T	2: 41,401,593 (GRCm39)	C240S	probably damaging	Het
Mapk8ip3	T	G	17: 25,131,781 (GRCm39)	E386A	probably damaging	Het
Mtmr14	A	G	6: 113,217,327 (GRCm39)	I80V	probably benign	Het
Nav2	A	C	7: 49,195,690 (GRCm39)	D961A	probably damaging	Het
Nmt2	A	G	2: 3,308,050 (GRCm39)	D107G	probably benign	Het
Nsd1	A	G	13: 55,394,681 (GRCm39)	K761E	probably damaging	Het
Ogdh	A	G	11: 6,298,678 (GRCm39)	T641A	probably benign	Het
Ogdhl	T	G	14: 32,054,783 (GRCm39)	F244V	possibly damaging	Het
Or51k1	G	A	7: 103,661,072 (GRCm39)	S279L	probably damaging	Het
Or5b102	A	T	19: 13,041,348 (GRCm39)	H191L	probably benign	Het
Or5b97	A	G	19: 12,879,030 (GRCm39)	I38T	probably benign	Het
Pfpl	T	C	19: 12,407,600 (GRCm39)	I617T	probably benign	Het
Ppfia2	T	G	10: 106,701,268 (GRCm39)		probably null	Het
Prickle1	T	C	15: 93,401,251 (GRCm39)	E411G	probably benign	Het
Prpsap2	C	A	11: 61,621,051 (GRCm39)	M343I	probably benign	Het
Prpsap2	A	G	11: 61,643,717 (GRCm39)	V72A	probably benign	Het
Ranbp17	A	T	11: 33,193,122 (GRCm39)	D977E	probably benign	Het
Rin1	T	C	19: 5,102,204 (GRCm39)	S238P	probably damaging	Het
Scap	C	T	9: 110,203,087 (GRCm39)	R254W	probably damaging	Het
Scn3a	T	C	2: 65,356,862 (GRCm39)	D194G	probably damaging	Het
Shisa7	T	C	7: 4,832,818 (GRCm39)	H481R	possibly damaging	Het
Slc6a6	T	C	6: 91,718,029 (GRCm39)	Y323H	probably damaging	Het
Thoc2l	A	G	5: 104,667,181 (GRCm39)	I568V	probably benign	Het
Tlr9	T	C	9: 106,101,140 (GRCm39)	S144P	probably benign	Het
Tmem69	T	A	4: 116,410,787 (GRCm39)	K61M	probably damaging	Het
Ttc21a	A	G	9: 119,770,331 (GRCm39)	D134G	probably damaging	Het
Ttn	A	G	2: 76,724,614 (GRCm39)		probably benign	Het
Vwf	A	G	6: 125,532,324 (GRCm39)	T26A	probably benign	Het
Zbtb16	A	T	9: 48,743,988 (GRCm39)	I108N	probably damaging	Het
Zc3h7b	T	A	15: 81,664,631 (GRCm39)	Y497N	probably damaging	Het
Zfp652	A	G	11: 95,640,155 (GRCm39)	S27G	probably damaging	Het
Zfp808	A	G	13: 62,320,666 (GRCm39)	T632A	possibly damaging	Het
Znhit6	T	C	3: 145,283,924 (GRCm39)		probably null	Het

Other mutations in Or2ag17

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Or2ag17	APN	7	106,389,796 (GRCm39)	missense	probably benign	0.12
IGL02093:Or2ag17	APN	7	106,390,030 (GRCm39)	missense	probably benign	0.12
IGL02404:Or2ag17	APN	7	106,389,566 (GRCm39)	missense	probably damaging	1.00
IGL03214:Or2ag17	APN	7	106,389,552 (GRCm39)	missense	probably benign
IGL03230:Or2ag17	APN	7	106,389,911 (GRCm39)	missense	probably damaging	1.00
R0194:Or2ag17	UTSW	7	106,390,030 (GRCm39)	missense	probably benign	0.12
R0523:Or2ag17	UTSW	7	106,389,533 (GRCm39)	missense	probably damaging	1.00
R1132:Or2ag17	UTSW	7	106,389,758 (GRCm39)	missense	possibly damaging	0.94
R1373:Or2ag17	UTSW	7	106,389,963 (GRCm39)	missense	probably benign	0.01
R1482:Or2ag17	UTSW	7	106,389,540 (GRCm39)	missense	probably benign	0.00
R1498:Or2ag17	UTSW	7	106,389,623 (GRCm39)	missense	possibly damaging	0.78
R1500:Or2ag17	UTSW	7	106,390,028 (GRCm39)	missense	probably damaging	1.00
R4163:Or2ag17	UTSW	7	106,389,486 (GRCm39)	missense	probably damaging	1.00
R4638:Or2ag17	UTSW	7	106,390,205 (GRCm39)	start codon destroyed	probably null	1.00
R5104:Or2ag17	UTSW	7	106,389,539 (GRCm39)	missense	possibly damaging	0.81
R6216:Or2ag17	UTSW	7	106,389,665 (GRCm39)	missense	probably benign	0.23
R6976:Or2ag17	UTSW	7	106,389,434 (GRCm39)	missense	probably damaging	0.99
R7129:Or2ag17	UTSW	7	106,389,690 (GRCm39)	missense	probably benign	0.00
R7130:Or2ag17	UTSW	7	106,389,389 (GRCm39)	missense	probably benign	0.35
R8104:Or2ag17	UTSW	7	106,390,338 (GRCm39)	start gained	probably benign
R8104:Or2ag17	UTSW	7	106,390,337 (GRCm39)	start gained	probably benign
R9057:Or2ag17	UTSW	7	106,389,296 (GRCm39)	missense	probably damaging	0.99
R9445:Or2ag17	UTSW	7	106,389,464 (GRCm39)	missense	probably damaging	1.00
Z1177:Or2ag17	UTSW	7	106,389,477 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGTGACAAGTGCTTTCTTCCTTC -3'
(R):5'- CACTTGGTGGTGCAGAAGAC -3'

Sequencing Primer
(F):5'- AAGTGCTTTCTTCCTTCCTTCATTTG -3'
(R):5'- GAAGACCTCCTTCTGGCCTTCATG -3'

Posted On

2014-12-04