Mutagenetix > Incidental Mutation

Incidental Mutation 'R2866:Mroh7'

253218

Institutional Source

Beutler Lab

Gene Symbol

Mroh7

Ensembl Gene

ENSMUSG00000047502

Gene Name

maestro heat-like repeat family member 7

Synonyms

Heatr8, Gm1027, LOC381538

MMRRC Submission

040455-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2866 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

106537614-106588122 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 106548287 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 1064 (G1064R)

Ref Sequence

ENSEMBL: ENSMUSP00000102382 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106770]

AlphaFold

A2AVR2

Predicted Effect

probably damaging
Transcript: ENSMUST00000106770
AA Change: G1064R

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000102382
Gene: ENSMUSG00000047502
AA Change: G1064R

Domain	Start	End	E-Value	Type
low complexity region	39	61	N/A	INTRINSIC
low complexity region	318	332	N/A	INTRINSIC
low complexity region	563	573	N/A	INTRINSIC
SCOP:d1b3ua_	634	1218	6e-9	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134160

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135000

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142954

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145374

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atmin	T	A	8: 117,683,112 (GRCm39)	D257E	probably benign	Het
Best1	T	C	19: 9,963,585 (GRCm39)	E532G	probably benign	Het
Cenpj	T	C	14: 56,789,637 (GRCm39)	H804R	probably benign	Het
Clec2g	T	C	6: 128,925,719 (GRCm39)	S43P	probably benign	Het
Col8a2	A	G	4: 126,204,992 (GRCm39)		probably benign	Het
Cpz	T	C	5: 35,659,705 (GRCm39)	K647E	probably benign	Het
Csmd2	T	C	4: 128,308,185 (GRCm39)		probably null	Het
Ctss	A	G	3: 95,452,717 (GRCm39)	K166R	probably benign	Het
Cyp2c23	T	C	19: 43,993,885 (GRCm39)	R494G	probably damaging	Het
Cyp2c68	A	G	19: 39,677,589 (GRCm39)	I467T	probably damaging	Het
Dcaf11	A	T	14: 55,803,202 (GRCm39)	T299S	possibly damaging	Het
Dennd1b	A	G	1: 139,098,019 (GRCm39)	S762G	possibly damaging	Het
Epb42	C	T	2: 120,856,402 (GRCm39)	A381T	possibly damaging	Het
Fhad1	A	G	4: 141,648,099 (GRCm39)	Y256H	probably benign	Het
Gfra1	C	T	19: 58,227,739 (GRCm39)	A395T	possibly damaging	Het
Gm10323	C	A	13: 67,002,574 (GRCm39)	C55F	probably benign	Het
Greb1	T	C	12: 16,749,551 (GRCm39)	S1092G	probably damaging	Het
Grid1	A	T	14: 35,284,516 (GRCm39)	D753V	probably damaging	Het
Grin2b	A	G	6: 135,710,637 (GRCm39)	F970L	probably damaging	Het
Kcnma1	A	T	14: 23,423,275 (GRCm39)	N682K	probably benign	Het
Lat2	T	A	5: 134,634,798 (GRCm39)	D114V	probably damaging	Het
Lcat	C	T	8: 106,666,511 (GRCm39)	C337Y	probably damaging	Het
Mapk10	C	T	5: 103,186,548 (GRCm39)	D25N	probably benign	Het
Muc21	T	C	17: 35,930,599 (GRCm39)		probably benign	Het
Or10h5	T	A	17: 33,435,252 (GRCm39)	H22L	probably benign	Het
Or51ah3	A	T	7: 103,210,064 (GRCm39)	I127F	probably damaging	Het
Or5p5	T	A	7: 107,414,126 (GRCm39)	C112S	probably benign	Het
Or8k39	A	T	2: 86,563,773 (GRCm39)	F61Y	possibly damaging	Het
Polr1has	A	T	17: 37,276,052 (GRCm39)	R211S	possibly damaging	Het
Psg27	T	A	7: 18,295,818 (GRCm39)	D209V	probably benign	Het
Ptgir	A	G	7: 16,640,794 (GRCm39)	M29V	possibly damaging	Het
Pzp	A	G	6: 128,502,227 (GRCm39)	S41P	possibly damaging	Het
Rab23	A	T	1: 33,777,376 (GRCm39)	K163N	possibly damaging	Het
Rilpl2	T	C	5: 124,615,898 (GRCm39)	D84G	probably damaging	Het
Sorl1	A	G	9: 41,881,077 (GRCm39)	I2148T	probably benign	Het
Tead1	A	G	7: 112,358,694 (GRCm39)	E2G	probably damaging	Het
Tigd4	A	G	3: 84,501,259 (GRCm39)	N59D	possibly damaging	Het
Tmprss15	T	A	16: 78,832,121 (GRCm39)	D345V	possibly damaging	Het
Togaram2	T	C	17: 72,016,592 (GRCm39)	S649P	probably benign	Het
Ucp2	T	C	7: 100,146,459 (GRCm39)	V95A	probably benign	Het
Usp17lb	T	C	7: 104,489,955 (GRCm39)	D323G	probably damaging	Het
Zfp677	A	T	17: 21,617,518 (GRCm39)	K192*	probably null	Het
Zmym2	T	A	14: 57,165,705 (GRCm39)	I676K	probably damaging	Het

Other mutations in Mroh7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01722:Mroh7	APN	4	106,560,358 (GRCm39)	missense	probably benign	0.00
IGL01729:Mroh7	APN	4	106,561,402 (GRCm39)	missense	possibly damaging	0.66
IGL01834:Mroh7	APN	4	106,538,071 (GRCm39)	missense	probably benign	0.00
IGL02003:Mroh7	APN	4	106,559,726 (GRCm39)	missense	probably damaging	0.96
IGL02135:Mroh7	APN	4	106,559,707 (GRCm39)	missense	probably damaging	1.00
IGL02335:Mroh7	APN	4	106,564,979 (GRCm39)	missense	probably damaging	1.00
IGL02532:Mroh7	APN	4	106,577,788 (GRCm39)	missense	probably benign	0.04
IGL02896:Mroh7	APN	4	106,557,013 (GRCm39)	missense	possibly damaging	0.94
IGL03066:Mroh7	APN	4	106,549,595 (GRCm39)	missense	possibly damaging	0.85
IGL03298:Mroh7	APN	4	106,571,288 (GRCm39)	nonsense	probably null
holy	UTSW	4	106,567,152 (GRCm39)	splice site	probably null
moley	UTSW	4	106,551,509 (GRCm39)	splice site	probably null
P0016:Mroh7	UTSW	4	106,565,054 (GRCm39)	critical splice acceptor site	probably null
R0019:Mroh7	UTSW	4	106,578,623 (GRCm39)	missense	probably benign	0.07
R0094:Mroh7	UTSW	4	106,560,381 (GRCm39)	missense	probably damaging	0.98
R0105:Mroh7	UTSW	4	106,568,467 (GRCm39)	missense	possibly damaging	0.49
R0105:Mroh7	UTSW	4	106,568,467 (GRCm39)	missense	possibly damaging	0.49
R0515:Mroh7	UTSW	4	106,548,861 (GRCm39)	missense	probably benign	0.01
R0828:Mroh7	UTSW	4	106,557,073 (GRCm39)	missense	probably damaging	0.99
R0831:Mroh7	UTSW	4	106,537,990 (GRCm39)	missense	possibly damaging	0.92
R1107:Mroh7	UTSW	4	106,564,791 (GRCm39)	splice site	probably null
R1301:Mroh7	UTSW	4	106,577,692 (GRCm39)	missense	probably damaging	0.99
R1456:Mroh7	UTSW	4	106,552,338 (GRCm39)	splice site	probably benign
R1491:Mroh7	UTSW	4	106,560,255 (GRCm39)	missense	probably benign	0.11
R1540:Mroh7	UTSW	4	106,560,273 (GRCm39)	missense	probably benign	0.11
R1560:Mroh7	UTSW	4	106,568,451 (GRCm39)	missense	possibly damaging	0.78
R1645:Mroh7	UTSW	4	106,577,865 (GRCm39)	missense	probably benign	0.19
R1804:Mroh7	UTSW	4	106,551,589 (GRCm39)	missense	possibly damaging	0.76
R2162:Mroh7	UTSW	4	106,557,378 (GRCm39)	missense	probably damaging	0.96
R2265:Mroh7	UTSW	4	106,578,124 (GRCm39)	missense	probably benign	0.01
R3716:Mroh7	UTSW	4	106,561,407 (GRCm39)	missense	probably benign	0.25
R3718:Mroh7	UTSW	4	106,561,407 (GRCm39)	missense	probably benign	0.25
R4530:Mroh7	UTSW	4	106,577,634 (GRCm39)	missense	possibly damaging	0.71
R4661:Mroh7	UTSW	4	106,548,710 (GRCm39)	critical splice donor site	probably null
R4706:Mroh7	UTSW	4	106,548,821 (GRCm39)	missense	possibly damaging	0.86
R4910:Mroh7	UTSW	4	106,567,152 (GRCm39)	splice site	probably null
R4965:Mroh7	UTSW	4	106,548,184 (GRCm39)	missense	possibly damaging	0.77
R4969:Mroh7	UTSW	4	106,538,070 (GRCm39)	missense	probably benign
R4971:Mroh7	UTSW	4	106,548,749 (GRCm39)	missense	probably benign	0.04
R5083:Mroh7	UTSW	4	106,547,515 (GRCm39)	missense	probably benign	0.03
R5207:Mroh7	UTSW	4	106,578,583 (GRCm39)	missense	probably damaging	0.97
R5364:Mroh7	UTSW	4	106,548,840 (GRCm39)	missense	probably benign	0.10
R5392:Mroh7	UTSW	4	106,568,448 (GRCm39)	critical splice donor site	probably null
R5630:Mroh7	UTSW	4	106,577,764 (GRCm39)	missense	possibly damaging	0.71
R5691:Mroh7	UTSW	4	106,559,815 (GRCm39)	missense	probably damaging	0.96
R5703:Mroh7	UTSW	4	106,565,757 (GRCm39)	missense	possibly damaging	0.77
R5707:Mroh7	UTSW	4	106,539,082 (GRCm39)	missense	possibly damaging	0.73
R5919:Mroh7	UTSW	4	106,551,509 (GRCm39)	splice site	probably null
R5979:Mroh7	UTSW	4	106,578,123 (GRCm39)	missense	probably benign	0.00
R6479:Mroh7	UTSW	4	106,560,385 (GRCm39)	missense	possibly damaging	0.75
R6520:Mroh7	UTSW	4	106,578,460 (GRCm39)	missense	probably benign	0.00
R6657:Mroh7	UTSW	4	106,559,697 (GRCm39)	nonsense	probably null
R6732:Mroh7	UTSW	4	106,537,910 (GRCm39)	frame shift	probably null
R6817:Mroh7	UTSW	4	106,571,312 (GRCm39)	missense	probably benign	0.00
R6980:Mroh7	UTSW	4	106,557,434 (GRCm39)	missense	probably benign	0.05
R7062:Mroh7	UTSW	4	106,541,177 (GRCm39)	missense	probably damaging	1.00
R7116:Mroh7	UTSW	4	106,568,517 (GRCm39)	missense	probably benign	0.07
R7134:Mroh7	UTSW	4	106,577,791 (GRCm39)	missense	probably damaging	0.99
R7169:Mroh7	UTSW	4	106,548,836 (GRCm39)	missense	probably damaging	0.99
R7419:Mroh7	UTSW	4	106,541,115 (GRCm39)	missense	probably benign
R7516:Mroh7	UTSW	4	106,548,316 (GRCm39)	missense	probably benign	0.00
R7525:Mroh7	UTSW	4	106,566,899 (GRCm39)	missense	probably benign	0.22
R7540:Mroh7	UTSW	4	106,577,595 (GRCm39)	missense	possibly damaging	0.85
R7849:Mroh7	UTSW	4	106,578,287 (GRCm39)	missense	probably benign
R7920:Mroh7	UTSW	4	106,564,773 (GRCm39)	missense	probably benign
R7998:Mroh7	UTSW	4	106,568,478 (GRCm39)	missense	probably benign	0.02
R8026:Mroh7	UTSW	4	106,578,634 (GRCm39)	missense	probably benign	0.01
R8122:Mroh7	UTSW	4	106,559,726 (GRCm39)	missense	probably damaging	0.96
R8249:Mroh7	UTSW	4	106,578,409 (GRCm39)	missense	probably benign	0.00
R9188:Mroh7	UTSW	4	106,566,789 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CAGTCCTGGCTTGGTCCTATAAAG -3'
(R):5'- GGTAGCCTAATGGTGAGCAC -3'

Sequencing Primer
(F):5'- CTATAAAGGAGGTGCCCTTCAG -3'
(R):5'- GTGAGCACCTAACTTAGACTCAGTG -3'

Posted On

2014-12-04