Mutagenetix > Incidental Mutation

Incidental Mutation 'R2866:Muc21'

253289

Institutional Source

Beutler Lab

Gene Symbol

Muc21

Ensembl Gene

ENSMUSG00000090588

Gene Name

mucin 21

Synonyms

epiglycanin, Gm9573

MMRRC Submission

040455-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R2866 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

35928815-35937529 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

T to C at 35930599 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134221 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164502] [ENSMUST00000174521]

AlphaFold

F7C950

Predicted Effect

unknown
Transcript: ENSMUST00000164502
AA Change: T1196A

SMART Domains

Protein: ENSMUSP00000130987
Gene: ENSMUSG00000090588
AA Change: T1196A

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
low complexity region	26	39	N/A	INTRINSIC
low complexity region	42	59	N/A	INTRINSIC
low complexity region	76	144	N/A	INTRINSIC
low complexity region	149	578	N/A	INTRINSIC
low complexity region	580	653	N/A	INTRINSIC
low complexity region	655	1179	N/A	INTRINSIC
low complexity region	1183	1373	N/A	INTRINSIC
low complexity region	1383	1436	N/A	INTRINSIC
low complexity region	1438	1479	N/A	INTRINSIC
Pfam:Epiglycanin_C	1518	1605	3.8e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173759

Predicted Effect

probably benign
Transcript: ENSMUST00000174521

SMART Domains

Protein: ENSMUSP00000134221
Gene: ENSMUSG00000090509

Domain	Start	End	E-Value	Type
Pfam:SFTA2	80	117	9.2e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174534

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atmin	T	A	8: 117,683,112 (GRCm39)	D257E	probably benign	Het
Best1	T	C	19: 9,963,585 (GRCm39)	E532G	probably benign	Het
Cenpj	T	C	14: 56,789,637 (GRCm39)	H804R	probably benign	Het
Clec2g	T	C	6: 128,925,719 (GRCm39)	S43P	probably benign	Het
Col8a2	A	G	4: 126,204,992 (GRCm39)		probably benign	Het
Cpz	T	C	5: 35,659,705 (GRCm39)	K647E	probably benign	Het
Csmd2	T	C	4: 128,308,185 (GRCm39)		probably null	Het
Ctss	A	G	3: 95,452,717 (GRCm39)	K166R	probably benign	Het
Cyp2c23	T	C	19: 43,993,885 (GRCm39)	R494G	probably damaging	Het
Cyp2c68	A	G	19: 39,677,589 (GRCm39)	I467T	probably damaging	Het
Dcaf11	A	T	14: 55,803,202 (GRCm39)	T299S	possibly damaging	Het
Dennd1b	A	G	1: 139,098,019 (GRCm39)	S762G	possibly damaging	Het
Epb42	C	T	2: 120,856,402 (GRCm39)	A381T	possibly damaging	Het
Fhad1	A	G	4: 141,648,099 (GRCm39)	Y256H	probably benign	Het
Gfra1	C	T	19: 58,227,739 (GRCm39)	A395T	possibly damaging	Het
Gm10323	C	A	13: 67,002,574 (GRCm39)	C55F	probably benign	Het
Greb1	T	C	12: 16,749,551 (GRCm39)	S1092G	probably damaging	Het
Grid1	A	T	14: 35,284,516 (GRCm39)	D753V	probably damaging	Het
Grin2b	A	G	6: 135,710,637 (GRCm39)	F970L	probably damaging	Het
Kcnma1	A	T	14: 23,423,275 (GRCm39)	N682K	probably benign	Het
Lat2	T	A	5: 134,634,798 (GRCm39)	D114V	probably damaging	Het
Lcat	C	T	8: 106,666,511 (GRCm39)	C337Y	probably damaging	Het
Mapk10	C	T	5: 103,186,548 (GRCm39)	D25N	probably benign	Het
Mroh7	C	T	4: 106,548,287 (GRCm39)	G1064R	probably damaging	Het
Or10h5	T	A	17: 33,435,252 (GRCm39)	H22L	probably benign	Het
Or51ah3	A	T	7: 103,210,064 (GRCm39)	I127F	probably damaging	Het
Or5p5	T	A	7: 107,414,126 (GRCm39)	C112S	probably benign	Het
Or8k39	A	T	2: 86,563,773 (GRCm39)	F61Y	possibly damaging	Het
Polr1has	A	T	17: 37,276,052 (GRCm39)	R211S	possibly damaging	Het
Psg27	T	A	7: 18,295,818 (GRCm39)	D209V	probably benign	Het
Ptgir	A	G	7: 16,640,794 (GRCm39)	M29V	possibly damaging	Het
Pzp	A	G	6: 128,502,227 (GRCm39)	S41P	possibly damaging	Het
Rab23	A	T	1: 33,777,376 (GRCm39)	K163N	possibly damaging	Het
Rilpl2	T	C	5: 124,615,898 (GRCm39)	D84G	probably damaging	Het
Sorl1	A	G	9: 41,881,077 (GRCm39)	I2148T	probably benign	Het
Tead1	A	G	7: 112,358,694 (GRCm39)	E2G	probably damaging	Het
Tigd4	A	G	3: 84,501,259 (GRCm39)	N59D	possibly damaging	Het
Tmprss15	T	A	16: 78,832,121 (GRCm39)	D345V	possibly damaging	Het
Togaram2	T	C	17: 72,016,592 (GRCm39)	S649P	probably benign	Het
Ucp2	T	C	7: 100,146,459 (GRCm39)	V95A	probably benign	Het
Usp17lb	T	C	7: 104,489,955 (GRCm39)	D323G	probably damaging	Het
Zfp677	A	T	17: 21,617,518 (GRCm39)	K192*	probably null	Het
Zmym2	T	A	14: 57,165,705 (GRCm39)	I676K	probably damaging	Het

Other mutations in Muc21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
BB009:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
FR4304:Muc21	UTSW	17	35,933,013 (GRCm39)	intron	probably benign
R0334:Muc21	UTSW	17	35,933,614 (GRCm39)	intron	probably benign
R0946:Muc21	UTSW	17	35,929,105 (GRCm39)	missense	probably benign	0.32
R1117:Muc21	UTSW	17	35,930,920 (GRCm39)	intron	probably benign
R1345:Muc21	UTSW	17	35,932,489 (GRCm39)	intron	probably benign
R1697:Muc21	UTSW	17	35,931,540 (GRCm39)	intron	probably benign
R1750:Muc21	UTSW	17	35,931,940 (GRCm39)	intron	probably benign
R1756:Muc21	UTSW	17	35,930,131 (GRCm39)	intron	probably benign
R1946:Muc21	UTSW	17	35,933,416 (GRCm39)	intron	probably benign
R1978:Muc21	UTSW	17	35,933,857 (GRCm39)	intron	probably benign
R1991:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R1992:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R2063:Muc21	UTSW	17	35,932,297 (GRCm39)	intron	probably benign
R2356:Muc21	UTSW	17	35,932,563 (GRCm39)	intron	probably benign
R3826:Muc21	UTSW	17	35,932,504 (GRCm39)	intron	probably benign
R4020:Muc21	UTSW	17	35,930,953 (GRCm39)	intron	probably benign
R4474:Muc21	UTSW	17	35,931,496 (GRCm39)	intron	probably benign
R4677:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R4786:Muc21	UTSW	17	35,930,221 (GRCm39)	intron	probably benign
R5071:Muc21	UTSW	17	35,931,444 (GRCm39)	intron	probably benign
R5173:Muc21	UTSW	17	35,931,633 (GRCm39)	intron	probably benign
R5283:Muc21	UTSW	17	35,932,224 (GRCm39)	intron	probably benign
R5446:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5542:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5716:Muc21	UTSW	17	35,931,675 (GRCm39)	intron	probably benign
R5913:Muc21	UTSW	17	35,934,123 (GRCm39)	intron	probably benign
R6011:Muc21	UTSW	17	35,933,074 (GRCm39)	intron	probably benign
R6198:Muc21	UTSW	17	35,931,808 (GRCm39)	intron	probably benign
R6394:Muc21	UTSW	17	35,931,058 (GRCm39)	intron	probably benign
R6786:Muc21	UTSW	17	35,934,057 (GRCm39)	intron	probably benign
R6940:Muc21	UTSW	17	35,934,118 (GRCm39)	intron	probably benign
R7082:Muc21	UTSW	17	35,932,093 (GRCm39)	missense	unknown
R7103:Muc21	UTSW	17	35,932,432 (GRCm39)	missense	unknown
R7110:Muc21	UTSW	17	35,933,510 (GRCm39)	intron	probably benign
R7139:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7165:Muc21	UTSW	17	35,932,870 (GRCm39)	missense	unknown
R7200:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7204:Muc21	UTSW	17	35,932,105 (GRCm39)	intron	probably benign
R7289:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7290:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7295:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7319:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R7462:Muc21	UTSW	17	35,931,568 (GRCm39)	missense	unknown
R7529:Muc21	UTSW	17	35,930,123 (GRCm39)	missense	unknown
R7718:Muc21	UTSW	17	35,933,728 (GRCm39)	missense	unknown
R7762:Muc21	UTSW	17	35,932,977 (GRCm39)	missense	unknown
R7788:Muc21	UTSW	17	35,929,798 (GRCm39)	missense	unknown
R7798:Muc21	UTSW	17	35,932,146 (GRCm39)	missense	unknown
R7831:Muc21	UTSW	17	35,929,651 (GRCm39)	missense	unknown
R7896:Muc21	UTSW	17	35,930,917 (GRCm39)	missense	unknown
R7899:Muc21	UTSW	17	35,931,493 (GRCm39)	intron	probably benign
R7932:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R8025:Muc21	UTSW	17	35,931,879 (GRCm39)	intron	probably benign
R8077:Muc21	UTSW	17	35,930,628 (GRCm39)	intron	probably benign
R8090:Muc21	UTSW	17	35,932,617 (GRCm39)	missense	unknown
R8169:Muc21	UTSW	17	35,932,072 (GRCm39)	missense	unknown
R8184:Muc21	UTSW	17	35,933,722 (GRCm39)	missense	unknown
R8209:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8226:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8464:Muc21	UTSW	17	35,933,098 (GRCm39)	intron	probably benign
R8670:Muc21	UTSW	17	35,932,540 (GRCm39)	missense	unknown
R8783:Muc21	UTSW	17	35,930,875 (GRCm39)	missense	unknown
R8856:Muc21	UTSW	17	35,931,865 (GRCm39)	missense	unknown
R9155:Muc21	UTSW	17	35,932,131 (GRCm39)	missense	unknown
R9214:Muc21	UTSW	17	35,931,838 (GRCm39)	missense	unknown
R9353:Muc21	UTSW	17	35,930,545 (GRCm39)	missense	unknown
R9618:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R9621:Muc21	UTSW	17	35,932,720 (GRCm39)	missense	unknown
R9679:Muc21	UTSW	17	35,930,491 (GRCm39)	missense	unknown
RF025:Muc21	UTSW	17	35,933,771 (GRCm39)	intron	probably benign
Z1176:Muc21	UTSW	17	35,932,137 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,951 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,817 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CCTGAGGCAGTGCTAGATTC -3'
(R):5'- ACTGAATCCAGCACTGCCTC -3'

Sequencing Primer
(F):5'- AGGCAGTGCTAGATTCAGTGG -3'
(R):5'- TCAGGCTCTACACCTACCCGG -3'

Posted On

2014-12-04